• 식물병연구
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• 제26권3호
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• pp.144-158
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• 2020

정량적역전사중합효소연쇄반응(quantitative reverse transcription PCR)은 정확하고 민감한 방법으로 유전자 발현분석에 사용된다. 사과 식물에서 유전자 발현 변화의 정량적 변화를 분석하기 위해, 사과 잎검은점 바이러스(Apple stem grooving virus, ASGV)에 의한 감염 동안 발현의 안정성에 대해 10개 참조유전자들(ACT, CKL, EF-1α, GAPDH, MDH, PDI, THF, UBC, UBC10 및 WD40)을 평가하였다. AGSV 감염 또는 저온 처리된 사과 식물에서의 10개 참조유전자 발현의 안정은 5가지 프로그램을 사용하여 분석하였다. ASGV 감염 사과식물의 잎 조직에서는 CKL>THFs>GAPDH>ACT 순서로 가장 안정한 유전자로 분석되었으며 WD40CKL>UBC10이고 가장 안정하지 않은 유전자는 ACT

• 한국안전학회지
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• 제34권5호
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• pp.111-118
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• 2019

Recently, a variety of special concrete structures have been designed in domestic and overseas construction markets and more advanced construction technology is required. Therefore, it is necessary to secure quantitative construction technology. For this purpose, it is essential to develop a standard reference material having a constant flow performance and quality to evaluate quantitative performance. On the other hand, the flowability of the concrete is greatly influenced by the flowability of the cement paste. Also, in consideration of design strength and workability, mix design is carried out at various mixing ratios according to the purpose of the site. Therefore, in this study, based on the derived components of standard reference materials for cement paste, we suggested mixing ratio of standard reference materials that can uniformly simulate the flow characteristics of cement paste according to W/C. As a result, it was found that the yield stress was determined by the ratio of water and glycerol but plastic viscosity was controled by limestone content. Finally, the ratio of standard reference materials to simulate the rheological range of cement paste by W/C was suggested.

• International Journal of Naval Architecture and Ocean Engineering
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• 제13권1호
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• pp.379-395
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• 2021

For the complicated structural details in ships and offshore structures, the traditional hotspot stress approaches are known to be sensitive to the element variables of element topologies, sizes, and integration schemes. This motivated to develop a new approach for predicting reasonable hotspot stresses, which is less sensitive to the element variables and easy to be implemented the real marine structures. The three-point bending tests were conducted for the longitudinal attachments with the round and rectangular weld toes. The tests were reproduced in the numerical simulations using the solid and shell element models, and the simulation technique was validated by comparing the experimental stresses with the simulated ones. This paper considered three hotspot stress approaches: the ESM method based on surface stress extrapolation, the Dong's method based on nodal forces along a weld toe, and the proposed method based on nodal forces perpendicular to an imaginary vertical plane at a weld toe. In order to study the element sensitivities of each method, 16 solid element models and 8 shell element models were generated under the bending and tension loads, respectively. The element sensitivity was analyzed in terms of Stress Concentration Factors (SCFs) in viewpoints of two statistical quantities of mean and bias with respect to the reference SCFs. The average SCFs predicted by the proposed method were remarkably in good agreement with the reference SCFs based on the experiments and the ship rules. Negligibly small Coefficients of Variation (CVs) of the SCFs, which is measure of statistical bias, were drawn by the proposed method.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 추계학술대회
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• pp.31-36
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• 2004

Defective components of interest include not only homogeneous components, but also components with weldments where tensile properties vary across the weldment. Noting that the region near the weldment is the most vulnerable place for crack initiation and subsequent growth, defect assessment methods for homogeneous structure. Moreover, weldment width and crack location also affects the deformation and fracture behavior of the welded joints. These weld characteristics can evaluate using plastic limit load. So in this paper, evaluate plastic limit load both full circumference part-throughwall cracked pipes and circumference through-wall cracked pipes considering weld characteristics. And using evaluate results, proposed J-integral and crack opening displacement(COD) estimate method based on reference stress method.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회A
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• pp.1766-1771
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• 2007

This paper presents plastic limit loads and approximate J-integral estimates for circumferential part-through surface crack at the interface between elbows and pipes. Based on finite element limit analyses using elastic-perfectly plastic materials, plastic limit moments under in-plane bending are obtained and it is found that they are similar those for circumferential part-through surface cracks in the center of elbow. Based on present FE results, closed-form limit load solutions are proposed. Welds are not explicitly considered and all materials are assumed to be homogeneous. And the method to estimate the elastic-plastic J-integral for circumferential part-through surface cracks at the interface between elbows and straight pipes is proposed based on the reference stress approach, which was compared with corresponding solutions for straight pipes.

• Journal of Radiation Protection and Research
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• 제29권1호
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• pp.49-55
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• 2004

This paper presents the mechanical and thermal stress analysis of a disposal canister to provide basic information for dimensioning the canister and configuration of the canister components. The structural stress analysis is carried out using a finite element analysis code, NISA, and focused on the structural strength of the canister against the expected external pressures due to the swelling of the bentonite buffer and the hydrostatic head, and the thermal load build up in the container.

• 대한기계학회논문집A
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• 제32권5호
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• pp.411-418
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• 2008

In this work, the effect of strength mismatch on plastic limit loads is quantified for similar metal weld plates with cracks under tension load, via three-dimensional, small strain elastic-perfectly plastic finite element analyses. Relevant variables related to plate geometry and crack length are systematically varied, in addition to the weld width. An important finding is that mis-match limit loads can be uniquely quantified through strength mis-match ratio and one geometry-related parameter. Based on the proposed limit load solutions, reference stress based J-integral estimates is also investigated. When the reference stress is defined by the mis-match limit load, predicted J-integral values agree overall well with FE results.

• 대한조선학회논문집
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• 제49권6호
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• pp.469-477
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• 2012

This paper describes the characteristic analysis of ice impact force for the Arctic structure shape. In the present study an energy method has been used to predict the impact force during the ice-structure collision. This study also employs two concepts for reference contact area and normalized stress in analysis procedure. The influences of factors, such as impact velocity, full penetration depth, structure shape and ice floe size, are investigated. Full penetration occurs, particularly at lower impact velocity when ice thickness increase. But "typical size" ice floe does not expected ever to achieve full penetration during the impact procedure. The structure shape is the dominant factor in ice impact force characteristic. The results for various ice-structure collision scenarios are analyzed.

• Journal of the Korean Data and Information Science Society
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• 제20권3호
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• pp.601-614
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• 2009

This paper derives noninformative priors for scale parameter of exponential distribution when the data are collected in multiple step stress accelerated life tests. We nd the objective priors for this model and show that the reference prior satisfies first order matching criterion. Also, we show that there exists no second order matching prior. Some simulation results are given and using artificial data, we perform Bayesian analysis for proposed priors.

• 대한치과보철학회지
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• 제43권2호
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• pp.204-217
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• 2005

Purpose. The purpose of this study was to analyze stress distribution of all ceramic posterior fixed partial denture using a three dimensional finite element method. Material and method. A three dimensional finite element model was created to demonstrate all-ceramic posterior fixed partial denture and then this computer model measured the stress distribution of the all ceramic bridges which has a ceramic core materials such as Zirconia, IPS Empress. 2. In-Ceram zirconia, Metal-Ceramic. Also the stress distribution was examined according to loading sites when force was applied to sites such as the central area of second premolar the mesial connector of pontic, the central fossa of pontic, the distal connector of pontic, and the central fossa of second molar. Results. 1. In all the materials of the core in this study, von Mises stress indicated that the stress increased as force was applied to loaded sites, just at those points, on the connector, and the margin in the area adjacent to the connectors. 2. The maximum principal stress was much higher in the lower part of the connectors than in any other region. 3. As the load was applied to the different locations, the research showed a consistent increase of stress in the lower connectors. The maximum value of the von Mises stress was two or three times greater when the load was applied directly to the connectors rather than indirectly through another stressed region. 4. In the case of In-Ceram zirconia, the stress in lower connectors was the highest of all the reference points, the stress showed 75% of all the maximum stress. Ziconia showed 72%, Metal Ceramic 67% and IPS Empress 2 50%. 5. In the case of Ziconia, the stress was well dispersed in each reference point that the stress differences were smaller when compared to In-Cream ziconia.