• Title/Summary/Keyword: Reference Population

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Detection of Mendelian and Parent-of-origin Quantitative Trait Loci for Meat Quality in a Cross between Korean Native Pig and Landrace

  • Choi, B.H.;Lee, Y.M.;Alam, M.;Lee, J.H.;Kim, T.H.;Kim, K.S.;Kim, J.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.12
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    • pp.1644-1650
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    • 2011
  • This study was conducted to detect quantitative trait loci (QTL) affecting meat quality in an $F_2$ reference population of Korean native pig and Landrace crossbreds. The three-generation mapping population was generated with 411 progeny from 38 $F_2$ full-sib families, and 133 genetic markers were used to produce a sex-average map of the 17 autosomes. The data set was analyzed using least squares Mendelian and parent-of-origin interval-mapping models. Lack-of-fit tests between models were used to characterize the QTL for mode of gene expressions. A total of 10 (32) QTL were detected at the 5% genome (chromosome)-wise level for the analyzed traits. Of the 42 QTL detected, 13 QTL were classified as Mendelian, 10 as paternal, 14 as maternal, and 5 as partial expressed QTL, respectively. Among the QTL detected at 5% genome-wise level, four QTL had Mendelian mode of inheritance on SSCs 5, 10, 12, and 13 for cooking loss, drip loss, crude lipid and crude protein, respectively; two QTL maternal inheritance for pH at 24-h and shear force on SSC11; three QTL paternal inheritance for CIE b and Hunter b on SSC9 and for cooking loss on SSC15; and one QTL partial expression for crude ash on SSC13, respectively. Most of the Mendelian QTL (9 of 13) had a dominant mode of gene action, suggesting potential utilization of heterosis for genetic improvement of meat quality within the cross population via marker-assisted selection.

Effects of insect-resistant genetically modified rice (Bt-9) cultivation on non-target insect diversity

  • Oh, Sung-Dug;Lim, Myung-Ho;Lee, Bumkyu;Yun, Doh-Won;Sohn, Soo-In;Chang, Ancheol;Park, Soon Ki;Suh, Sang Jae
    • Korean Journal of Agricultural Science
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    • v.45 no.1
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    • pp.28-37
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    • 2018
  • This study was done to develop environmental risk assessments and a biosafety guide for insect-resistant genetically modified rice at a LMO (Living Modified Organism) isolation field. In the LMO quarantine area of Kyungpook National University, the species diversities and population densities of non-target insects found on insect-resistant genetically modified rice (Bt-9) resistant to Cnaphalocrocis medinalis and on non-GM rices (Dongjin and Ilmi) were investigated. The Bt-9 event was therefore evaluated under field conditions to detect possible impacts on the above ground insects and spiders. The study compared transgenic rice and two non-GM reference rices, Ilmi and Dongjin, at Gunwi in Southern Korea in 2016. Each rice was grown on three $18m^2$ plots with a randomized block design. A total of 4,243 individuals from 43 families and 9 orders were collected from the LMO isolation field. In the three types of rice fields, a total of 1,467 individuals from the insect-resistant genetically modified rice (Bt-9), 1,423 individuals from the Ilmi, and 1,353 individuals from the Dongjin were collected, respectively. There was no difference between the population densities of the non-target insect pests, natural enemies and other insects on the insect-resistant genetically modified rice (Bt-9) and non-GM rices. These results provide the diversity and population density of non-target insects for an environment risk assessment survey on insect-resistant genetically modified rice and could be used as a guideline to make a biosafety assessment method for genetically modified crops.

A comparative study on the estimation methods for the potential yield in the Korean waters of the East Sea (한국 동해 생태계의 잠재생산량 추정방법에 관한 비교 연구)

  • LIM, Jung-Hyun;SEO, Young-Il;ZHANG, Chang-Ik
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.54 no.2
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    • pp.124-137
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    • 2018
  • Due to the decrease in coastal productivity and deterioration in the quality of ecosystem which result from the excessive overfishing of fisheries resources and the environmental pollution, fisheries resources in the Korean waters hit the dangerous level in respect of quantity and quality. In order to manage sustainable and effective fisheries resources, it is necessary to suggest the potential yield (PY) for clarifying available fisheries resources in the Korean waters. So far, however, there have been few studies on the estimation methods for PY in Korea. In addition, there have been no studies on the comparative analysis of the estimation methods and the substantial estimation methods for PY targeted for large marine ecosystem (LME) For the reasonable management of fisheries resources, it is necessary to conduct a comprehensive study on the estimation methods for the PY which combines population dynamics and ecosystem dynamics. To reflect the research need, this study conducts a comparative analysis of estimation methods for the PY in the Korean waters of the East Sea to understand the advantages and disadvantages of each method, and suggests the estimation method which considered both population dynamics and ecosystem dynamics to supplement shortcomings of each method. In this study, the maximum entropy (ME) model of the holistic production method (HPM) is considered to be the most reasonable estimation method due to the high reliability of the estimated parameters. The results of this study are expected to be used as significant basic data to provide indicators and reference points for sustainable and reasonable management of fisheries resources.

A Study on the Foot and Ankle Disease of Shelter-based Homeless People (노숙인 거주 시설의 족부 족관절 실태에 대한 조사)

  • Min, Hak-Jin;Kim, Ki Chun;Kim, Jae Woo
    • Journal of Korean Foot and Ankle Society
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    • v.21 no.4
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    • pp.156-164
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    • 2017
  • Purpose: Foot and ankle disease (FAD) is a frequent cause of morbidity among the homeless population. Various conditions, exacerbated by malnutrition, poor lifestyle habits, psychiatric disorders, physical injuries, poor hygiene, and limited access to healthcare, have been described in this population. The purpose of this study was to investigate the cognition and management status of FAD in shelter based homeless people. Materials and Methods: Fifty-two male and twenty-five female volunteer homeless individuals were recruited from two homeless shelters. Each person completed a questionnaire assessing any presence of pain, pain management, as well as foot and ankle care status. A foot and ankle surgeon examined the physical status of the individual's foot and ankle, including tenderness and instability. A radiologic evaluation was done for 18 male and 11 female homeless people who agreed to participate in this test for the existence osteophyte or joint space narrowing representing osteoarthritis and some reference angles for hallux valgus, flatfoot and cavus foot. Results: Homeless people had higher prevalence of body mass index, diabetes, and smoking than the general population. The most prevalent infectious disease was fungal infection (male 78%, female 68%), with a low compliance of management for FAD. Conclusion: Although most of shelter-based homeless people showed an acceptable foot and ankle management status, the potential risk for FAD development and exacerbation of mild FAD was high.

Ecological Characteristics of Picea abies Plantation in Comparison with Fagus sylvatica Population (유럽너도밤나무 군락과 독일가문비 조림지의 생태특성 비교)

  • Lee, Young-Geun;Cho, Hyun-Je
    • The Korean Journal of Ecology
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    • v.23 no.5
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    • pp.377-382
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    • 2000
  • Picea abies plantation in Mt. Eifel, Germany was studied phytosociologically, with special reference to species composition, structure, ecology and dormancy form, comparing to those of Fagus sylvastica population that once dominated the P. abies plantation. Thirteen plant species appeared on average. According to their ecological distribution, they were classified into three groups, F sylvastica forests, natural coniferous forests and bare-land after clear-cutting. In Luzulo-Fagetum population, Luzula luzuloides was the dominated species while in P. abies plantation was dominated by Avenella flesuosa. In assessment of herbaceous plants by indicator species of Ellenberg, increasing number of shade-intolerant species and acid-tolerant species occurred. In analysis of plant dormancy form, species of Chamaephytes, Phanerophytes and Hemikryptophytes increased, while that of Geophytes decreased. As a result, we found P. abies plantation unstable in ecological aspects.

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Evaluation of Inbreeding and Genetic Variability of Five Pig Breeds in Czech Republic

  • Krupa, Emil;Zakova, E.;Krupova, Z.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.1
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    • pp.25-36
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    • 2015
  • The complex analysis of the pedigree records of Czech Landrace (CLA), Czech Large White-dam line (CLWd), Czech Large White-sire line (CLWs), Duroc (DC), and Pietrain (PN) was performed to determine trends of genetic diversity (GD), and to find the main sources of the GD loss. The total size of the pedigree was 132,365, 391,151, 32,913, 13,299, and 7,160 animals in CLA, CLWd, CLWs, DC, and PN, respectively. Animals born in the years 2011 through 2013 were assumed as the reference population. The average pedigree completeness index for one generation back was 95.9%, 97.4%, 91.2%, 89.8%, and 94.2% for appropriate breeds. Number of ancestors explaining 100% of gene pool was 186, 373, 125, 157, and 37 in CLA, CLWd, CLWs, DC, and PN, respectively. The relative proportion of inbred animals (58%, 58%, 54%, 47%, and 25%), the average inbreeding (2.7%, 1.4%, 2.5%, 3.6%, and 1.3%) and the average co-ancestry (3.1%, 1.6%, 3.3%, 4.2%, and 3.3%) were found over the past decade in analysed breeds. The expected inbreeding under random mating increased during the last 10 years in CLWs and PN and varied from 1.27% to 3.2%. The effective population size computed on the basis of inbreeding was 76, 74, 50, 35, and 83 in 2012 in CLA, CLWd, CLWs, DC, and PN, respectively. The shortest generation interval (1.45) was observed for CLWd in sire to son selection pathway. The longest generation interval obtained PN (1.95) in sire to daughter pathway. The average relative GD loss within last generation interval was 7.05%, 4.70%, 9.81%, 7.47%, and 10.46%, respectively. The relative proportion of GD loss due to genetic drift on total GD loss was 85.04%, 84.51%, 89.46%, 86.19%, and 83.68% in CLA, CLWd, CLWs, DC, and PN, respectively. All breeds were characterized by a high proportion of inbred animals, but the average inbreeding was low. The most vulnerable breeds to loss of GD are DC and PN. Therefore, a breeding program should be more oriented to prevent the increase of GD loss in these breeds.

Identification of Causal and/or Rare Genetic Variants for Complex Traits by Targeted Resequencing in Population-based Cohorts

  • Kim, Yun-Kyoung;Hong, Chang-Bum;Cho, Yoon-Shin
    • Genomics & Informatics
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    • v.8 no.3
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    • pp.131-137
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    • 2010
  • Genome-wide association studies (GWASs) have greatly contributed to the identification of common variants responsible for numerous complex traits. There are, however, unavoidable limitations in detecting causal and/or rare variants for traits in this approach, which depends on an LD-based tagging SNP microarray chip. In an effort to detect potential casual and/or rare variants for complex traits, such as type 2 diabetes (T2D) and triglycerides (TGs), we conducted a targeted resequencing of loci identified by the Korea Association REsource (KARE) GWAS. The target regions for resequencing comprised whole exons, exon-intron boundaries, and regulatory regions of genes that appeared within 1 Mb of the GWA signal boundary. From 124 individuals selected in population-based cohorts, a total of 0.7 Mb target regions were captured by the NimbleGen sequence capture 385K array. Subsequent sequencing, carried out by the Roche 454 Genome Sequencer FLX, generated about 110,000 sequence reads per individual. Mapping of sequence reads to the human reference genome was performed using the SSAHA2 program. An average of 62.2% of total reads was mapped to targets with an average 22X-fold coverage. A total of 5,983 SNPs (average 846 SNPs per individual) were called and annotated by GATK software, with 96.5% accuracy that was estimated by comparison with Affymetrix 5.0 genotyped data in identical individuals. About 51% of total SNPs were singletons that can be considered possible rare variants in the population. Among SNPs that appeared in exons, which occupies about 20% of total SNPs, 304 nonsynonymous singletons were tested with Polyphen to predict the protein damage caused by mutation. In total, we were able to detect 9 and 6 potentially functional rare SNPs for T2D and triglycerides, respectively, evoking a further step of replication genotyping in independent populations to prove their bona fide relevance to traits.

Evaluation of Total Fat and Fatty Acids Intakes in the Korean Adult Population using Data from the 2016-2017 Korea National Health and Nutrition Examination Surveys (우리나라 성인의 총 지방 및 지방산 섭취량 평가: 2016-2017년 국민건강영양조사 자료를 활용하여)

  • Song, SuJin;Shim, Jae Eun
    • Korean Journal of Community Nutrition
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    • v.24 no.3
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    • pp.223-231
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    • 2019
  • Objectives: This study evaluated dietary intakes of total fat and fatty acids among the Korean adult population. Methods: This cross-sectional study used the 2016-2017 Korea National Health and Nutrition Examination Survey data. A total of 10,772 subjects aged ${\geq}19y$ for which dietary data were available were selected. Data pertaining to energy and nutrient intakes were obtained by a 24-h recall method. Total fat and fatty acids intakes were evaluated based on the Acceptable Macronutrient Distribution Ranges (AMDR) of 2015 Dietary Reference Intakes for Koreans by sex and age groups. All statistical analyses accounted for the complex sampling design effect and sampling weights. Results: The mean intakes of energy and total fat were 1,952 kcal (95% CI: 1928-1977) and 46.1 g (45.2-47.1), respectively, and about 21% of the energy was obtained from fat in this study population (21.7% in men and 20.2% in women). The mean percentages of energy from saturated, monounsaturated, and polyunsaturated fatty acids were 6.4%, 6.7%, and 5.2%, respectively. About 18% of adults exceeded the AMDR for fat (30% of energy), whereas 37.6% exceeded the AMDR for saturated fatty acids (7% of energy). The proportions of subjects who consumed more than the AMDR for fat and saturated fatty acids decreased across age groups in both sexes. Among young adults (19-29 y), about 63% of the subjects obtained ${\geq}7%$ of their energy from saturated fatty acids. About 61% of older adults obtained less than 15% of their energy from total fat. Conclusions: Increased intake of fat energy was prominent in saturated fatty acids. Our findings suggest current information on total fat and fatty acids intakes in Korean adults and can be used to provide dietary guidelines for the improvement of public health.

intake of dietary sugar and its influence on chronic disease in the korean population (한국인의 당류 섭취현황과 만성질환에 미치는 영향)

  • Ha, Kyungho;Joung, Hyojee;Song, YoonJu
    • Food Science and Industry
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    • v.49 no.3
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    • pp.2-11
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    • 2016
  • As recently many studies on the relationship between excessive sugar intake and chronic disease have been reported across the world, more attention to sugar intake has been paid. Sugars, called simple sugars, is a kind of carbohydrate and sum of monosaccharides and disaccharides. Sugars that are particularly related to health are added sugars or free sugars, which are added to food by processing or cooking. It is generally not to give satiety and increases energy density without other nutrients. Especially, the major food source of added sugar is sugar-sweetened beverages(SSBs), from which sugars are closely linked with health outcome. The total sugar intake in Korea is 61.4 g per day and it is almost half of those in the western countries such as the U.S. or Australia and it is within the recommendation level by the Dietary Reference Intakes for Koreans. However, when it is classified by age groups or food sources, sugar intake in adolescents and young adults are high and the sugar from processed foods is also high that would lead to high intake levels of added sugar. Particularly, the frequency of SSBs, the major food source of added sugar, is the highest among 20's. While the studies of excessive sugar intake with chronic diseases including obesity, type 2 diabetes, metabolic disease, cardiovascular disease reported in the Western populations, those in the Korean populations have just started. Given our dietary practice is very different from those in the western population, more prospective studies would be necessary to evaluate sugar intake by type or food source exactly and examine its relationship with chronic diseases in the Korean population.

A Study on the Effect of Korean Medicine Health Promotion Project on Budgeting (한의약건강증진사업의 예산편성여부에 미치는 영향 연구)

  • Kim, Yun Hwan;Han, Hyosang
    • Journal of Digital Convergence
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    • v.20 no.4
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    • pp.699-704
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    • 2022
  • The purpose of this study is to determine the relating factors of the Budgeting for Korean Medicine health promotion in the Integrated Community Health Promotion Program as of 2020 targeting 226 basic local governments nationwide. Descriptive statistics, Difference tests, and Binary logistic regression analysis were conducted to analyze the factors relating Budgeting for Korean Medicine health promotion in the Integrated Community Health Promotion Program in local communities using regional budgets in 2020. As a result of the analysis, the difference in the Budgeting for Korean Medicine Infertility Treatment Support, Number of Korean Medicine Doctor, Number of Population people over 65 years old, and Aging Population Percentage. And there was a influencing factors, Budgeting for Korean Medicine Infertility Treatment Support, Aging Population Percentage, Whether to join a healthy city, so expected to be used as a reference in organizing policies and business budgets related to Korean Medicine in the future.