• Journal of Microbiology and Biotechnology
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• 제18권4호
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• pp.624-630
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• 2008

Terminal restriction fragment length polymorphism (T-RFLP) is a method that has been frequently used to survey the microbial diversity of environmental samples and to monitor changes in microbial communities. T-RFLP is a highly sensitive and reproducible procedure that combines a PCR with a labeled primer, restriction digestion of the amplified DNA, and separation of the terminal restriction fragment (T-RF). The reliable identification of T-RF requires the information of nucleotide sequences as well as the size of T-RF. However, it is difficult to obtain the information of nucleotide sequences because the T-RFs are fragmented and lack a priming site of 3'-end for efficient cloning and sequence analysis. Here, we improved on the T-RFLP method in order to analyze the nucleotide sequences of the distinct T-RFs. The first method is to selectively amplify the portion of T-RF ligated with specific oligonucleotide adapters. In the second method, the termini of T-RFs were tailed with deoxynucleotides using terminal deoxynucleotidyl transferase (TdT) and amplified by a second round of PCR. The major T-RFs generated from reference strains and from T-RFLP profiles of activated sludge samples were efficiently isolated and identified by using two modified T-RFLP methods. These methods are less time consuming and labor-intensive when compared with other methods. The T-RFLP method using TdT has the advantages of being a simple process and having no limit of restriction enzymes. Our results suggest that these methods could be useful tools for the taxonomic interpretation of T-RFs.

• Journal of Periodontal and Implant Science
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• 제45권6호
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• pp.216-222
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• 2015

Purpose: After extraction, the alveolar bone tends to undergo atrophy in three-dimensions. The amount of alveolar bone loss in the horizontal dimension has been reported to be greater than the amount of bone loss in the vertical dimension, and is most pronounced in the buccal aspect. The aim of this study was to monitor the predictive alveolar bone level following the extraction of anterior teeth seriously involved with advanced chronic periodontitis. Methods: This study included 25 patients with advanced chronic periodontitis, whose maxillary anterior teeth had been extracted due to extensive attachment loss more than one year before the study. Periapical radiographs were analyzed to assess the vertical level of alveolar bone surrounding the edentulous area. An imaginary line connecting the mesial and the distal ends of the alveolar crest facing the adjacent tooth was arbitrarily created. Several representative coordinates were established in the horizontal direction, and the vertical distance from the imaginary line to the alveolar crest was measured at each coordinate for each patient using image analysis software. Regression functions predicting the vertical level of the alveolar bone in the maxillary anterior edentulous area were identified for each patient. Results: The regression functions demonstrated a tendency to converge to parabolic shapes. The predicted maximum distance between the imaginary line and the alveolar bone calculated using the regression function was $1.43{\pm}0.65mm$. No significant differences were found between the expected and actual maximum distances. Likewise, the predicted and actual maximum horizontal distances did not show any significant differences. The distance from the alveolar bone crest to the imaginary lines was not influenced by the mesio-distal spans of the edentulous area. Conclusions: After extraction, the vertical level of the alveolar ridge increased to become closer to the reference line connecting the mesial and distal alveolar crests.

• 한국측량학회지
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• 제31권6_2호
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• pp.567-576
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• 2013

The determination of the geoid in South Korea is a national imperative for the modernization of height datums, specifically the orthometric height and the dynamic height, that are used to monitor hydrological systems and environments with accuracy and easy revision, if necessary. The geometric heights above a reference ellipsoid, routinely obtained by GPS, lead immediately to vertical control with respect to the geoid for hydrological purposes if the geoid height above the ellipsoid is known accurately. The geoid height is determined from gravimetric data, traditionally ground data, but in recent times also from airborne data. This paper illustrates the basic concepts for combining these two types of data and gives a preliminary performance assessment of either set or their combination for the determination of the geoid in South Korea. It is shown that the most critical aspect of the combination is the gravitational effect of the topographic masses above the geoid, which, if not properly taken into account, introduces a significant bias of about 8 mgal in the gravity anomalies, and which can lead to geoid height bias errors of up to 10 cm. It is further confirmed and concluded that achieving better than 5 cm precision in geoid heights from gravimetry remains a challenge that can be surmounted only with the proper combination of terrestrial and airborne data, thus realizing higher data resolution over most of South Korea than currently available solely from the airborne data.

• International journal of advanced smart convergence
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• 제6권4호
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• pp.9-18
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• 2017

In order to respond to the ever-changing media environments in the era of smart and mobile technology, KBS has introduced and partially applied PIE-TV and PIE-nonTV modes that monitor the average number of viewers among the national population group by means of the sample household extraction method which is a traditional way of rating investigation. This study analyzes the correlation between the number of viewers of premiere, re-air broadcasting, and MPP channel programs and the number of OTT-based VOD viewers in reference to the data extracted from PIE-TV survey results. KBS conducted a survey for 3 months between June and August 2017 to measure the PIE-TV Index, based on which the above-mentioned correlation was analyzed with programs classified to entertainment, drama, and cultural programs. For data analysis, SPSS (Ver. 18.0 for Window, SPSS Inc, Chicago, IL, USA) was utilized. It was assumed that when p<0.05 in the confidence interval of 95%, statistically significance would be secured. Among the 30 subjects in the simple correlation analysis, the parameter was determined by the Person correlation coefficient and the non-parameter by the Spearman correlation coefficient. Analysis results are as below: (1) As the number of viewers of premier entertainment, drama, and cultural programs was larger, the number of VOD viewers was larger accordingly. (2) As for entertainment and drama programs, as the number of re-air broadcasting viewers was larger, the number of VOD viewers decreased accordingly. (2) As for entertainment and drama programs, as the number of MPP viewers was larger, the number of VOD viewers decreased accordingly. It is expected that this statistical data can be utilized for strategic planning of MPP channel lineups including terrestrial TV broadcasting, cable TV, etc.

• 대한원격탐사학회지
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• 제29권5호
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• pp.537-544
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• 2013

남극 세종 과학 기지가 위치하고 있는 바톤반도는 눈과 식생이 주를 이루고 있고, 기후변화와 같은 환경변화에 민감하게 반응한다. 극지역의 지표 모니터링은 기후변화 이해를 위해 중요하다. 그러나 극 지역은 접근성 및 공간규모로 인해 지속적으로 모니터링 하기에 어려움이 있다. 위성영상은 지속적으로 동일지역을 모니터링 할 수 있다는 장점과 함께 다중분광영역을 이용하여 지표의 상태를 파악하는데 효율적이다. 따라서 본 연구에서는 바톤반도의 지표의 상태를 지속적으로 모니터링하기 위한 기초자료로 KOMPSAT-2 다중 분광 위성영상을 이용하여 토지피복분류를 수행하였고, 나아가 분류된 토지피복 중 식생 종의 분포를 파악하였다. 다중분광영상인 KOMPSAT-2 위성영상과 현장관측자료를 이용하여 계층적 분류를 수행하였고 정확도를 평가하였다. 전반적으로 식생지역과 비식생 지역이 명확하게 분류되었으나 식생 종 분류에는 낮은 정확도를 보였다.

• 지능정보연구
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• 제9권1호
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• pp.53-69
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• 2003

현대의 생산공정에서는 많은 공정변수가 발생하고 있으며 복잡한 연관관계를 가지고 제품의 품질에 영향을 미치고 있다. 따라서 공정의 이상 유무 확인을 위해서는 많은 품질특성치를 동시에 관리하는 다변량 공정관리가 필요하다. 본 연구는 자기조직화 신경망(SOM)과 사례기반추론(CBR) 기법을 이용한 다변량 공정관리 방안을 제안한다. SOM을 이용하여 공정 데이터의 패턴을 생성하고 이상 유무 판단을 위해 기준패턴과 적합성 검정을 한다. 제안한 방법의 검증을 위해 공정에서 발생 가능한 패턴별로 데이터를 생성하여 실험하였고, 실험을 통해 이상패턴을 효과적으로 구별할 수 있음을 보였다. 또한 CBR 방법론을 적용하여 1종 오류는 줄이면서 2종 오류를 아주 작게 유지할 수 있음을 보임으로써, SOM과 CBR 이 결합된 절차가 다변량 공정관리를 위한 한 대안이 될 수 있음을 보였다.

• 한국전자거래학회지
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• 제9권1호
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• pp.39-59
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• 2004

e-비즈니스는 기업활동을 자동화하고 업무시스템 간에 정보소통을 가능하게 하였다. 이러한 자동화와 정보공유는 기업간 환경으로 확장되어, 거래 기업간에 비즈니스프로세스와 교환 메시지를 정의하여 거래과정을 자동화하려는 시도가 발생하였다. 본 연구에서 다루고 있는 기업간 워크플로우 통합은 이러한 시도 중의 하나로서, 기업의 개별적인 워크플로우를 중심으로 추가적인 기업간 활동을 정의함으로써 기업간 비즈니스 프로세스로 확장하는 시스템 통합 방법이다. 본 연구에서는 먼저 워크플로우 통합을 위한 요구사항 및 관련 표준과 통합 기술에 대하여 살펴보고, 이를 바탕으로 기업간 워크플로우 통합을 위한 참조모델을 제시한다. 그리고 관련 표준과 기술을 바탕으로 기업간 워크플로우 통합을 구현하기 위한 세 가지 접근 방법을 제시하였다. 세 가지 접근법은 워크플로우 시스템을 중심으로 상호운영하는 방법, 웹 서비스를 통해 공적 프로세스를 구현하는 방법, 그리고 내부 프로세스와 외부 프로세스를 독립적으로 운영하면서 다중 프로세스를 구현하는 방법이다. 또한, 참조 모델을 바탕으로 각 접근 방법의 구현을 위한 아키텍처를 제공한다. 이러한 연구는 기업간 워크플로우 통합에 대한 기업의 이해를 돕고 관련 표준 및 기술을 활용하여 시스템 통합을 구현하는 데 도움이 될 것이다.

• Journal of Astronomy and Space Sciences
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• 제24권4호
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• pp.269-274
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• 2007

강한 지자기 폭풍이 발생하였을 때 한반도 상공의 전리층내 총전자수(TEC, Total Electron Contents) 변화를 분석하기 위해 GPS 기준국망을 데이터를 이용한 지역적인 전리층 감시모델을 개발하였다. 전리층 총전자수 감시모델 개발을 위해 한국천문연구원에서 운용중인 대전 IGS(International GNSS Service) 기준국을 포함한 전국에 고르게 분포하고 있는 9개의 GPS 기준국 데이터를 이용하였다. 또한 순간적인 전리층 변화 특성을 분석하기 위해 CSS(Cubic Spline Smoothing)기법을 적용하였고, 그 결과 2003년 11월 20일 강한 지자기 폭풍이 발생했을 때 한반도 상공에서 총전자수의 순간적인 변화를 검출할 수 있었다. 이때에는 평일과 비교했을 때 특정시각의 약 1.5배 이상 총전자수가 증가함을 보였다. 마지막으로 지구 자기장 활동 정도를 나타내는 Kp 지수, Dst 지수 그리고 천문연 GPS 기준국망 데이터를 이용해 산출된 총전자수 변화와의 연관성을 제시했다.

• 대한방사선치료학회지
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• 제11권1호
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• pp.6-10
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• 1999

The field size can be beam output, therefore MonitorUnit can be varied due to field size dependence The purpose of this study is to evaluate and compare the dose variation according to exchange of collimator The measurements were perfomed with Wellhofer dosimetry system(water phantom. ion chamber. electrometer. system controller. build up cap. etc)and two types of linear accerlerator (Mevatron KD, MevatronMX) Scatter can be affected to field size dependence and scatter correction is separated into collimator and phantom components, scatter components can affect by exchanging of collimator Measurements of collimator scatter factor(Sc) was done in air with build up cap. 1)Square field (5cm2 to 40cm2) was measured 2)and then keeping the upper jaw constant at loom and varing lower jaw from 5cm to 40cm, 3)keeping the lower jaw constant at 10cm and varing upper jaw from 5cm to 40cm Measurements of total scatter factor(Scp) was done in water at Dmax as the procedure of collimator scatter factor measurements in water Dmax The total scatter factors were obtained to the following equation(Sp=Scp/Sc) The measured data is normalized to the data of reference field size($10{\times}10$), rectangular field is inverted to equivalent field to compare three field size data As the collimator setting is varied, the output was changed In conclusion, the error was obtained small but it must be eliminated if we intend to reach the common stated goal of $5\%$ overall uncertainty in dose determination

• 대한의용생체공학회:의공학회지
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• 제35권6호
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• pp.177-184
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• 2014

Continuous monitoring of heart rates and respiratory rates for newborns or infants is very important since the abnormal breathing and heart problems can threaten the life of newborns or infants. A noncontact baby monitoring system based on a Doppler radar and an air mattress was designed. The Doppler radar was used to acquire respiratory signals and the air mattress was employed to obtain heart rates. The performance of the designed system was evaluated using a commercialized infant simulator ($Simbaby^{TM}$) and a respiration belt transducer was used to measure respiration rates as a reference. Results for respiratory rates revealed that the correlation coefficients between I-and Q-channel and the respiration belt were 0.84 and 0.91 and the mean ${\pm}$ standard deviations of errors between them were $1.66{\pm}1.92$ (bpm) and $0.88{\pm}1.65$ (bpm). Heart rates showed that the correlation coefficient between air mattress and set value of the simulator was 0.73 and the mean ${\pm}$ standard deviation of errors between them was $1.09{\pm}3.45$ (bpm). These results indicate that the designed system holds the potential as an effective monitoring tool for continuous monitoring heart rates and respiratory rates of newborns or infants.