• Asian-Australasian Journal of Animal Sciences
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• v.26 no.12
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• pp.1665-1671
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• 2013

Real-time quantitative PCR (qRT-PCR) is one of the important methods for investigating the changes in mRNA expression levels in cells and tissues. Selection of the proper reference genes is very important when calibrating the results of real-time quantitative PCR. Studies on the selection of reference genes in goat tissues are limited, despite the economic importance of their meat and dairy products. We used real-time quantitative PCR to detect the expression levels of eight reference gene candidates (18S, TBP, HMBS, YWHAZ, ACTB, HPRT1, GAPDH and EEF1A2) in ten tissues types sourced from Boer goats. The optimal reference gene combination was selected according to the results determined by geNorm, NormFinder and Bestkeeper software packages. The analyses showed that tissue is an important variability factor in genes expression stability. When all tissues were considered, 18S, TBP and HMBS is the optimal reference combination for calibrating quantitative PCR analysis of gene expression from goat tissues. Dividing data set by tissues, ACTB was the most stable in stomach, small intestine and ovary, 18S in heart and spleen, HMBS in uterus and lung, TBP in liver, HPRT1 in kidney and GAPDH in muscle. Overall, this study provided valuable information about the goat reference genes that can be used in order to perform a proper normalisation when relative quantification by qRT-PCR studies is undertaken.

• Korean Journal of Veterinary Service
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• v.42 no.2
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• pp.121-126
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• 2019

Metabolic profile test is used to evaluate nutritional imbalance and metabolic disease in dairy cows. The reference intervals of metabolic parameters may change according to nation, region, decades, and maintenance system. Despite the need to be periodically updated for the reference intervals of metabolic parameters, it has rarely been investigated in Korea. Therefore, this aim of study was to provide the reference intervals of metabolic parameters using dairy cows surveyed in Korea during recent years. A metabolic profile test was conducted for 2,976 clinically healthy dairy cows in Korea. Blood samples were collected for the analysis of serum metabolites. This study provided reference intervals of thirteen metabolic parameters (${\beta}$-hydroxybutyrate [${\beta}-HB$], non-esterified fatty acids [NEFA], glucose, total cholesterol [T-COL], total protein, albumin, globulin, blood urea nitrogen [BUN], aspartate aminotransferase [AST], gamma-glutamyl transferase [GGT], calcium, phosphorus, and magnesium). BUN and AST values of the current study were higher than those of previous studies. In the present study, the other metabolic parameters showed low or similar value compared to previous results. Moreover, ${\beta}-HB$, NEFA, T-COL, ALB, BUN, AST, and GGT values were affected by lactation period. This study provided information on the reference intervals of metabolites in healthy dairy cows in Korea. The reference intervals from the present study would be useful in managing and diagnosing disease of dairy cows. However, careful attention should be given in interpreting disease condition for metabolites affected by lactation.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.23 no.3
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• pp.8-17
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• 2015

In this research, low fidelity air/heat load analysis was conducted for the intake of high speed vehicle. For air/heat load calculations, aerodynamic properties at the surface and the boundary layer edge were estimated using Taylor-Maccoll equation for conical flow, shockwave relation and Prandtl-Meyer expansion equation for internal and external flow. Couette flow assumption and Reynolds analogy were used in order to calculate convective heat transfer coefficient. In order to calculate skin friction coefficient for heat transfer coefficient analysis, Van Driest method II and Reference Enthalpy method were considered. An axis symmetric SCRAMJET model was selected as a reference configuration for verifying the proper implementation of the present method. Comparison of the results using the present method and Computational Fluid Dynamic analysis showed that the present method is valuable for efficiently providing pressure and heat loads for air-intake structure design of the high speed air vehicle.

• Journal of the Korea Computer Industry Society
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• v.4 no.4
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• pp.479-490
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• 2003

CBD is a software development approach based on reusable component and supports easy modification and evolution of software. For the success of this approach, a component must be developed with high cohesion and low coupling. In this paper, we propose the two types of clustering analysis technique based on affinity between use-cases and classes and propose component identification method applying to this technique. We also propose component reference model and CBD methodology framework and perform a ease study to demonstrate how the affinity-based clustering technique is used in component identification method. Component identification method contains three tasks such as component extraction, component specification and component architecting. This method uses object-oriented concept for identifying component, which improves traceability from analysis to implementation and can automatically extract component. This method reflects the low coupling-high cohesion principle for good modularization about reusable component.

• Journal of Applied Reliability
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• v.16 no.2
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• pp.147-154
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• 2016

Purpose: To evaluate statistical differences among three measurements of range of motion (ROM) with Rapael Smart Glove (RSG) group 1, 2 and manual goniometer group. To investigate reference value of the kinematic analysis for range of motion (ROM) of distal upper extremity with Rapael Smart Glove (RSG). Methods: Sixteen normal persons without limitation of motion (LOM) enrolled in the study. The study was performed at two separate times and by two investigators on 16 normal adults. We compared ROM with RSG for measuring joint angles. We compared degrees of forearm supination/pronation, wrist flexion/extension and radial deviation/ulnar deviation during ROM of 16 participants using RSG. After one week, degrees of each motion were measured in the same way by other investigator to evaluate the reliability. Results: Statistical differences among three groups were showed. Most results of paired t-test between two RSG groups were over 0.05 and exceptions are supination, extension, and finger %. Conclusion: Our findings demonstrate that ROM of normal persons obtained by kinematic analysis with RSG are not valid as normal reference value for distal upper extremity motion. But, the reliability of between two RSG groups was showed with paired t-test and Pearson's correlation except supination, extension and finger %.

• Research in Community and Public Health Nursing
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• v.28 no.1
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• pp.13-22
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• 2017

Purpose: The aim of this study is to examine the reciprocal causal relationship between social activities and health with reference to the cognitive function level among community-dwelling older adults. Methods: We conducted a longitudinal data analysis using the $4^{th}$ (Time 1) and $5^{th}$ (Time 2) waves from the Korean Longitudinal Study of Ageing adopting cross-lagged panel analysis (CLPA). Results: A total of 3,473 community-dwelling older adults were included in the analysis: 2,053 in the normal cognitive function group, 912 in the mild cognitive impairment group, and 508 in the moderate to severe cognitive impairment group. The CLPA revealed that higher levels of social activities at Time 1 significantly influenced better subjective health perception at Time 2 in all three groups. In addition, better subjective health perception at Time 1 significantly influenced higher levels of social activities at time 2 only in the normal cognitive function group. Conclusion: This study demonstrates that participating in social activities has positive effects on health regardless of the cognitive function level in community-dwelling older adults. This finding suggests that there is a need to develop strategies that can be used to encourage older adults with cognitive decline to maintain participation in social activities.

• Steel and Composite Structures
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• v.23 no.1
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• pp.31-40
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• 2017

This article aims at presenting multi objective optimization of parameters that affect crashworthiness characteristics of bi-tubular structures using Taguchi method with grey relational analysis. To design the experiments, the $L_9$ orthogonal array has been used and based on that, the inner tubes have been fabricated by varying the three influence factors such as reference diameter, length difference and numbers of sides of the polygon with three levels, but all the outer cylinders have the same diameter and length 90 mm and 135 mm respectively. Then, the tailor made bi-tubular steel structures were subjected into quasi static axial compression. From the test results it is found that the crushing behaviors of bi-tubular structures with different combinations were fairly significant. The important responses (crashworthiness indicators) specific energy absorption and crush force efficiency have been evaluated from load - displacement curve. Finally optimal levels of parameters were identified using grey relational analysis, and significance of parameters was determined by analysis of variance. The optimum crashworthiness parameters are reference diameter 80 mm, length difference 0 mm and number of sides of polygon is 3, i.e., triangle within the selected nine bi-tube combinations.

• Korean Journal of Veterinary Research
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• v.60 no.3
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• pp.123-131
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• 2020

Portable blood glucose meters (PBGMs) are widely used because of their practicality. However, the accuracy of PBGMs has frequently been questioned. The objectives of this study were to evaluate factors that might interfere with measurements made using PBGMs, and to assess the clinical utility of 6 PBGMs. The glucose concentrations measured using the PBGMs were compared with those obtained using a reference method. The agreement between the measured values was assessed using Spearman correlation analysis, Passing-Bablok regression analysis, Bland-Altman plots, and consensus error grid analysis. Mann-Whitney and Kruskal-Wallis tests were performed to identify the parameters affecting glucose measurement. The results indicated that all of the PBGMs tested perform adequately for use in veterinary practice. In most cases, measurements made using PBGM corresponded well with the blood glucose values obtained using the reference method. Error grid analysis revealed that most of the PBGM values fell within zones A and B. However, some measurements of blood glucose concentrations < 80 mg/dL fell into zone C. PCV, and triglyceride and total protein concentration, significantly affected the output of some of the PBGMs. Therefore, clinicians should be aware of the characteristics of the PBGM that they use.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.30 no.1
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• pp.20-27
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• 2002

Aircraft design requires the intergration of several disciplines, inculding aerodynamics, structures, controls. To achieves advances in performance, each technology, or discipline must be more accurate in analysis and must be more highly intergrated. One of the important interdisciplinary interactions in mordern aircraft design is that of aerodynamics and structures. In this study, for increasing accuracy in each discipline's analysis, CFD for aerodynamic analysis and FEM for structurral analysis was used and, for considering important interdisciplinary interactions, aeroelastic effect was considered. As optimization algorithm, PBIL algorithm was used for global optima and was parallelized to alleviate the computational burden. The efficiency and accuracy of the present method was assesed by range maximiziation of reference of reference wing.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.30 no.6
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• pp.546-553
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• 2008

Purpose: The purpose of this study was to estimate validity of posterior anterior cephalometric and 3D-CT for orbital canting analysis. Materials and methods: Three trained observers classified two patients group using standardized frontal photographs of facial asymmetry patients. Group A consisted of patients with facial asymmetry and orbital canting(n=19), and group B consisted of patients with only facial asymmetry(n=43). Orbital canting was measured with line of bilateral inferior orbitale. Orbital canting measurement was done with posterior anterior cephalometric and 3D-CT. Each horizontal reference line was established by bilateral GWSO(cephalometric), FZS(3D-CT). Maxillary canting and mandibular deviation angle were also measured and analyzed with orbital canting. Results: The mean orbital canting was $3.03{\pm}1.00^{\circ}$ in Group A and $1.11{\pm}0.76^{\circ}$ in Group B in frontal photograph. The mean orbital canting was $1.20{\pm}0.74^{\circ}$ in group A and $1.22{\pm}0.65^{\circ}$ in group B by cephalometric analysis(p>0.05). In 3D-CT, orbital canting was almost paralleled with horizontal reference line. The orbital canting, maxillay canting and mandibular deviation between two groups showed no significant differences except madibular deviation in 3D-CT. Conclusion: Common analysis of posterior anterior cephalometric and 3D-CT is not valide method to evaluate orbital canting for facial asymmetry patients with orbital canting.