• 한국응용과학기술학회지
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• 제33권4호
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• pp.741-750
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• 2016

The acai berry (Euterpe oleracea Mart.) contains vitamin B complex, vitamin C, anthocyanin and so on. Especially acai berry was seen as nutritionally comparable to blueberry and related berries. The acai berry has significant aging-reducing properties. Compounds have been found to have anti-aging and antioxidant components. Acai berry was extracted with 70% methanol, 70% ethanol and CM (chloroform:methanol=2:1, v/v). After sample and reagents of each experiment was reacted, DPPH radical scavenging activity and ABTS radical scavenging activity, ferric reducing antioxidant power, reducing power were measured to determine the antioxidant capacity, and as results of comparing each extract. Ethanol (70%) extraction was measured highest. Anthocyanin, total phenol, flavonoid also appeared similar to the results. In addition, the antioxidant activities of the extraction solvents were increased significantly with increasing concentrations, but showed lower antioxidant activity than the positive control (ascorbic acid). As a result, antioxidant activities of sample supposed to affect by the anthocyanin, phenol and flavonoid contents.

• Preventive Nutrition and Food Science
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• 제16권1호
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• pp.89-94
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• 2011

The potential biological activities of water and ethanol extracts from two varieties of Rubus coreanus Miquel fruits produced in the Gochang-gun (GR) and Hoengseong-gun (HR) regions of Korea were examined. The hydroxyl radical ($\cdot$OH) scavenging activity, reducing power, lipid peroxidation inhibitory activity, and antiproliferative activity on cancer cells by these extracts were examined, and $\alpha$-amylase and $\alpha$-glucosidase inhibition assays were also performed. The EtOH extract from GR showed high hydroxyl radical scavenging activity ($EC_{50}=119.47{\pm}5.13\;{\mu}g/mL$), lipid peroxidation inhibitory activity ($EC_{50}=213.45{\pm}3.14\;{\mu}g/mL$) and a concentration dependence, with OD values ranging from 0.15 to 0.56 (50 to 200 ${\mu}g/mL$), for reducing power. The EtOH extract from GR has the highest antiproliferative activities on MDA-MB-231 and HepG2 cancer cells among four extracts. Meanwhile, all extracts showed certain $\alpha$-amylase and $\alpha$-glucosidase inhibition activities. These results indicate that extracts from two varieties of R. coreanus fruits have significant antioxidant, anti-diabetic and anti-tumorigenic activities, which suggest these extracts could be a potential source for pharmaceutical.

• Fisheries and Aquatic Sciences
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• 제21권10호
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• pp.33.1-33.11
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• 2018

As a process for commercial application, production of reducing sugar, antioxidant, and DNA protective compounds from shrimp-shell powder was investigated in a fed-batch biodegradation using Bacillus cereus EW5. The fed-batch biodegradation was operated in a 5-L bioreactor for 96 h according to three times pulse-feeding strategy. On the basis of the equal working volume (3 L), the fed-batch biodegradation showed a better production of the target compounds than the batch biodegradation, with higher cell density and shortened biodegradation period. The maximum values of the target compounds were 0.297 mg/mL of reducing sugar, 92.35% DPPH radical scavenging activity, 98.16% ABTS radical scavenging activity, and 1.55 reducing power at $A_{700}$, which were approximately 12.1, 3.4, 5.2, and 8.4% enhanced, respectively, compared with those obtained from the batch biodegradation. The fed-batch culture supernatant also showed the enhanced DNA damage inhibition activity than the batch culture supernatant. As a result, the fed-batch biodegradation accompanied by high cell density could produce more useful compounds, enabling an increase in the reutilization value of shrimp-shell waste.

• 한국응용과학기술학회지
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• 제32권4호
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• pp.631-638
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• 2015

In Korea, yam with thermal treatment is widely used to produce tea and beverage, which appeals to all age groups. To better understand the antioxidative activity of thermal treatment yam (Dioscorea batatas $D_{ECNE.}$), n-butanol and ethyl acetate extracts were evaluated with different antioxidant testing system. Their antioxidant activities, including ABTS [2,2' -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] radical scavenging activity, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, and ferric reducing antioxidant power as well as total phenol contents were assessed in vitro. Results showed ethyl acetate extract ($51.63{\pm}2.51mg\;CAE/g$) possessed similar total phenol content compared with n-butanol extract ($53.93{\pm}1.00mg\;CAE/g$). Ethyl acetate extract also exhibited similarly stronger antioxidant activity compared with n-butanol extract apart from ABTS radical scavenging activity assay.

• Preventive Nutrition and Food Science
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• 제14권4호
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• pp.277-282
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• 2009

The antioxidant potency of methanolic extract of Duchesnea indica (MDI; Indian strawberry) was investigated by employing various established in vitro systems, such as total phenolic content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, reducing power assay, metal chelating assay, superoxide radical scavenging activity and protective ability of DNA damage and protein oxidation. MDI inhibited metal chelating by 75.57% at 2 mg/mL, scavenged 50% DPPH free radical at 29.13 ${\mu}$g/mL, and eliminated approximately 46.21% superoxide radical at the concentration of 1 mg/mL. In addition, MDI showed strong ability on reducing power, DNA damage protection and protein oxidation protection. Overall, results suggested that MDI might be beneficial as a potent antioxidant and effectively employed as an ingredient in food applications.

• 한국자원식물학회지
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• 제23권3호
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• pp.207-213
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• 2010

Hovenia dulcis Thunb fruits were successively extracted with hot water, water, methanol, ethyl acetate, and chloroform. The crude extracts were investigated for potential antioxidant by measuring scavenging against DPPH free radicals, reducing power, superoxide radicals, and protection of protein damage and cultured cells from a lethal dose of hydrogen peroxide ($H_2O_2$). In all chemical assays used, the hot water extract of H. dulcis fruits, which contained $61.14{\pm}2.57$ (Tannic acid mg/g extract, n=3) of total phenolic compounds contents exhibited highest activity in in vitro models of DPPH free radical scavenging activity, reducing power assay, superoxide radical scavenging activity and protection of protein damage. In addition, the hot water extract protected cultured RAW 264.7 macrophages from a lethal dose of $H_2O_2$ and reduced reactive oxygen species level in RAW 264.7 cells.

• KSBB Journal
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• 제25권1호
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• pp.55-61
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• 2010

To investigate antioxidant activity of the culture broth from submerged culture of Tremella fuciformis, we preferentially analyzed the chemical composition of culture broth, which was mainly composed of carbohydrate (296.39 mg/g) and protein (9.24 mg/g), respectively. Also, contents of polyphenols, flavonoids and flavonols were 16.63 mg/g, 9.19 mg/g and 83.74 ${\mu}/g$, respectively. Next, we examined the scavenging abilities on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical, the reducing power, and chelating ability on ferrous ions. All antioxidant activities of the culture broth were increased in proportion to its concentrations. The $IC_{50}$ values were in order as follows ABTS radical scavenging activity < OPPH radical scavenging activity < chelating power. Accordingly, these results suggest that pharmacological function of T. fuciformis might be due to, at least partially, their protective effects against oxidation and the culture broth of T. fuciformis was free radical inhibitors or scavengers at low concentration, involving possibly in termination of free radical reaction as primary antioxidants.

• 동아시아식생활학회지
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• 제21권6호
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• pp.882-887
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• 2011

민들레 뿌리를 이용한 기능성 약주 개발의 기초 자료로 활용하고자 민들레 뿌리를 각각 0, 5, 10, 15%로 첨가하여 민들레 약주(기본적특성: pH 4.0~4.2, 산도4.6~4.8%, 알코올14.92~15.78%, 민들레 뿌리 5% 첨가한 약주를 가장 선호함)의 총 플라보노이드와 총 폴리페놀 함량 및 DPPH 라디칼 소거 활성을 이용한 항산화력을 측정하였으며, 활성 산소종 및 유리기에 전자를 공여하는 환원력을 조사하였다. 총 플라보노이드와 총 폴리페놀 함량은 민들레 뿌리 분말 첨가 비율이 증가할수록 높아졌다. 또한, DPPH 라디칼 소거 활성과 환원력에서도 민들레 뿌리 가루의 첨가비율이 높을수록 증가하였다. 이상의 결과로 보아 민들레 뿌리 가루를 첨가한 약주는 높은 생리활성물질과 항산화 활성을 가지고 있어 기능성 약주로서 개발이 가능하리라 판단된다.

• 한국식품영양과학회지
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• 제33권9호
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• pp.1419-1425
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• 2004

본 연구는 이팝나무 잎의 용매분획물을 이용하여 DPPH 소거 작용, 환원력과 같은 항산화 활성과 tyrosinase 저해 활성을 실험한 결과 에틸 아세테이트 분획물에서 높은 항산화 및 tyrosinase 저해 활성을 나타내었다. 따라서 silica gel column chromatography와 MPLC를 이용하여 그 활성물질을 분리하였으며, 분리한 화합물은 $^1H$, $^{13}C$-NMR 및 2DNMR을 이용하여 luteolin-4'-O-glucoside로 구조동정을 하였다. 분리한 luteolin-4'-O-glucoside와 기존 항산화제와의 비교실험에서 DPPH 라디칼 소거 활성과 환원력은 시료의 농도가 증가함에 따라 활성이 증가하였다. 순수하게 분리한 luteolin-4'-O-glucoside를 이용하여 tyrosinase 저해 활성도 농도 의존적이었으며, $IC_{50}$ 값은 23.2 ${\mu}g/mL$로 나타났다. 그러므로 luteolin-4'-O-glucoside는 식품첨가물로 항산화제로서의 활용도 및 미백효과를 지니는 기능성 화장품원료로서 이용가치가 높을 것으로 생각된다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2003년도 하계종합학술대회 논문집 II
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• pp.1153-1156
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• 2003

In this paper, we present a reducing power consumption of a scheduling for module selection under the time constraint. Traditional high-level synthesis do not allow reuse of complex, realistic datapath component during the task of scheduling. On the other hand, the proposed scheduling of reducing power consumption is able to approach a productivity of the design the low power to reuse which given a library of user-defined datapath component and to share of resource sharing on the switching activity in a shared resource. Also, we are obtainable the optimal the scheduling result in experimental results of our approach various HLS benchmark environment using chaining and multi-cycling in the scheduling techniques..