• Asian-Australasian Journal of Animal Sciences
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• v.13 no.11
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• pp.1561-1567
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• 2000

An experiment was conducted to investigate the effect of dietary protein levels and supplementation of phytase and pentosanase in wheat-soybean meal diet on the performance and output of N and P in broilers. Addition of phytase alone or in combination with pentosanase to reduced or control protein diets did not affect average final body weight of mixed sexes. However, addition of phytase and pentosanase in combination to reduced protein diets in male broilers significantly depressed body weights. Intestinal viscosity of 21d broilers was significantly decreased by addition of phytase and pentosanase alone or in combination. Tibia ash content was significantly increased by phytase supplementation. Supplementation of phytase alone and in combination with pentosanase to reduced protein diets significantly decreased P in manure and daily output of P. Daily N output was lowest in the reduced protein diet supplemented with phytase and pentosanase combination. The retention of DM, N and P was highest in the reduced protein diet supplemented with phytase and pentosanase combination. In conclusion, supplementation of phytase alone or in combination with pentosanase to reduced protein diets can decrease output of N and P. But the combination of the enzymes has no beneficial effects on the performance of broilers, especially those on wheat-soybean meal diet with reduced protein level.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.12
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• pp.1743-1749
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• 2000

An experiment was conducted to investigate the effect of supplementation of commercial phytase and ${\beta}-glucanase$ to wheat-soybean meal based layer diets. Control (17% CP) and reduced protein (13.5% CP) diets were compared with and without phytase and/or ${\beta}-glucanase$. Reducing dietary crude protein levels reduced the amount of N excreted by laying hens with no adverse affect on egg production or overall feed conversion ratio. There was, however, a slight reduction in average egg weight. When phytase was added to the control protein diets it was possible to reduce the level of dicalcium phosphate in the diet without a loss in performance and daily P output was reduced significantly. When phytase was added to the reduced protein diets, however, there was a dramatic loss in performance in the last four weeks of the study. Supplementation of ${\beta}-glucanase$ to wheat based layer diet did not appear to have beneficial affects in terms of laying performance and reducing nitrogen or phosphorus excretion. Combination of phytase and ${\beta}-glucanase$ had no positive effects on laying performance or reduction of DM, N and P.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.8
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• pp.1158-1164
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• 2003

In two feeding experiments male and mixed-sex broiler chicks were offered diets based on sorghum and a wheatsorghum blend with two tiers of nutrient specifications, without and with microbial phytase (600 and 800 FTU/kg), from 7-25 and 1-42 days post-hatch, respectively. The nutrient specifications for protein, amino acids, energy density and phosphorus (P) of standard diets were reduced to formulate the modified diets on a least-cost basis. Calculated differences in nutrient specifications between standard and modified diets ranged from 14.3 to 17.1 g/kg crude protein, 0.24 to 0.40 MJ/kg apparent metabolisable energy (AME) and 1.06 to 1.20 g/kg available P. In both experiments, reduced nutrient specifications had a negative impact on growth rates and feed efficiency and phytase supplementation had a positive influence on growth performance and protein efficiency ratios (PER). Phytase addition to the less expensive, modified diets either partially or entirely compensated for reduced growth performance and, consequently, feed costs per kg of live weight gain were reduced. In Experiment 1, phytase increased (p<0.001) nitrogen-corrected AME (AMEn) from 15.39 to 15.89 MJ/kg dry matter. For nitrogen (N) retention there was an interaction (p<0.05) between diet type and phytase as the effects of phytase on N retention were more pronounced in the modified diets, with an increase from 0.512 to 0.561. These results demonstrate the positive effects of phytase on protein and energy utilisation, in addition to its established liberation of phytate-bound P and illustrate the feasibility of assigning nutrient replacement values to the feed enzyme for consideration in least-cost ration formulations. Further work is, however, required to define the most appropriate reductions in nutrient specifications in association with phytase supplementation.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.3
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• pp.406-409
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• 2002

The effect of refeeding with various single essential amino acids on the recovery of plasma insulin-like growth factor-I (IGF-I) concentration in fasted young chickens was examined. Young chickens (29 days of age) were divided into 15 experimental groups. Chickens in one group were fed on the commercial diet ad libitum for 4 days. The remaining 56 chickens in 14 experimental groups were fasted. After 2 days of fasting, 52 chicks in 13 fasted groups were refed with one of the following experimental diets for 2 days. Eleven experimental diets were protein-free diets supplemented with one of 11 essential amino acids (Arg, Gly, His, Ileu, Leu, Met, Phe, Lys, Thr, Trp, Val). The remaining 2 experimental diets were a protein-free diet containing 11 essential amino acids and a protein-free diet not supplemented with amino acids. Birds in the remaining fasted group continued to be fasted for 2 days. Fasting for 2 days markedly reduced plasma IGF-I concentration. When fasted chickens were refed the protein-free diet containing either Gly alone or all essential amino acids, plasma IGF-I concentration was recovered to the level similar to that of fed chickens. Protein-free diet alone, however, failed to restore the reduced IGF-I concentration in plasma. Body weight loss modulated by feeding with protein-free diets supplemented with various single essential amino acids was associated with changes in plasma IGF-I concentrations. We concluded that body weight loss by feeding with a protein-free diet was lower than that of fasted chickens and that body weight loss associated with the decrease in plasma IGF-I concentration was modulated by feeding with protein-free diets containing various single essential amino acids.

• Preventive Nutrition and Food Science
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• v.1 no.2
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• pp.239-243
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• 1996

Three hundred and twenty-four 1 day old chicks were used to determine the effects of fat source and energy to protein ratio on growth performance , carcass composition and the efficiency on nutrient utilization. Chicks were assigned. in a completely randomized design, to 3*3 factorial arrangement of treatments. Chicks received one of three fat sources (n0 fat, tallow, corn oil) and one of three energy to protein ratios(16, 14 and 12kcal ME/g CP). All diets were formulated to be isocaloric(3.2Mcal ME/kg diets) using published ME values for the diet ingredients. Addition of tat to the diet increased ADG, average daily feed intake, and gain to feed, Chicks fed diets containing fat had increased percentage body DM and ether extract(EE), but percentage CP was not different, Chicks fed diets containing fat had increased efficiency of protein and energy deposition. Addition of fat ad either fallow or corn oil yielded similar results. Reducing the energy to protein ratio of the diet did not affect ADG or gain to feed, but tended to decrease average daily feed intake(p=0.80), as well as resulting in linear(p<0.05) reductions in body percentage DM., EE and also total EE. Increasing the energy to protein ratio did not affect percentage or total body Cp. Adding fat to poultry diets improved growth performance and the efficiency of growth chicks. Decreasing the energy to protein ratio did not affect growth performance, but reduced EE in the body of Chicks.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.9
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• pp.1317-1326
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• 2015

This study investigated the metabolizable energy (ME) intake, net energy of production (NEp), heat production (HP), efficiencies of ME use for energy, lipid and protein retention as well as the performance of broiler chickens fed diets based on cassava chips or pellets with or without supplementation with an enzyme product containing xylanase, amylase, protease and phytase. The two products, cassava chips and pellets, were analysed for nutrient composition prior to feed formulation. The cassava chips and pellets contained 2.2% and 2.1% crude protein; 1.2% and 1.5% crude fat; and 75.1% and 67.8% starch, respectively. Lysine and methionine were 0.077%, 0.075%, and 0.017%, 0.020% protein material, respectively, while calculated ME was 12.6 and 11.7 MJ/kg, respectively. Feed intake to day 21 was lower (p<0.01) on the diet containing cassava chips compared to diets with cassava pellets. Enzyme supplementation increased (p<0.01) feed intake on all diets. Live weight at day 21 was significantly (p<0.01) reduced on the diet based on cassava chips compared to pellets, but an improvement (p<0.01) was noticed with the enzyme supplementation. Metabolizable energy intake was reduced (p<0.01) by both cassava chips and pellets, but was increased (p<0.01) on all diets by enzyme supplementation. The NEp was higher (p<0.01) in the maize-based diets than the diets containing cassava. Enzyme supplementation improved (p<0.01) NEp in all the diets. Heat production was highest (p<0.01) on diets containing cassava pellets than on cassava chips. It is possible to use cassava pellets in diets for broiler chickens at a level close to 50% of the diet to reduce cost of production, and the nutritive value of such diets can be improved through supplementation of enzyme products containing carbohydrases, protease, and phytase.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.9
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• pp.1367-1375
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• 2008

This study was conducted to investigate the effects of phytase in diets containing cottonseed and soybean meal (CS) on growth performance, feed utilization and digestibility of protein and phosphorus in juvenile olive flounder (initial body weight 2.5 g), Paralichthys olivaceus. Four experimental diets replacing 0%, 30%, 30% and 40% fish meal protein with CS in equal proportion were formulated to be isonitrogenous and isocaloric (designated as CS0, CS30, CS30+P, CS40+P, respectively). Phytase of 1,000 FTU/kg was supplemented in diets CS30+P and CS40+P. Three groups of fish (25 fish per group) were fed one of the experimental diets for 10 weeks. No significant differences were observed in growth performance of fish groups except for the CS40+P diet. Apparent digestibility coefficients of protein and phosphorus in fish fed phytase-containing diets were significantly higher than those of fish fed the CS0 diet. Serum cholesterol was significantly reduced in fish fed the CS-containing diets. Antioxidant activities in the diets and liver of fish were significantly increased with the increment of dietary CS. Gossypol was only detected and found in liver of the fish fed the CS-containing diets. The findings suggest that supplementation of microbial phytase could improve the apparent digestibility of protein and phosphorus in juvenile olive flounder fed the CS-containing diets.

• Journal of Nutrition and Health
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• v.15 no.2
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• pp.119-128
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• 1982

The effect of dietary protein on growth and lipid levels of plasma and liver was studied in weanling male rats fed diets differing protein sources and amino acid balance. Rats were devided into 9 experimental diets which were grouped into 3 categories ; 1) Simple protein category includes gluten-, soy protein isolate-, and casein-containing diet groups, 2) Supplemented category includes casein supplemented with methionine, soy protein isolate supplemented with methionine, and gluten supplemented with lysine and methionine, 3) Mixed protein category includes diet groups containing gluten (2/3), casein (1/3), soy protein isolate (2/3) and casein (1/3), and casein (1/3), soy protein isolate (1/3) and gluten (1/3). The experimental diets composed of 15% protein, 65.8% carbohydrate, 10% fat and 1% cholesterol. The body wt. gain and P.E.R. were greater in rats of supplemented and mixed protein groups than simple protein groups. No statistical differences were found in plasma cholesterol among gluten, soy protein isolate and casein groups. Consumption of diets supplemented with limiting amino acid to gluten or soy protein isolate reduced the plasma cholesterol level by 23.2% and 34.2% respectively. However there was no difference between casein and the supplemented casein groups. The mixed protein groups shows relatively high plasma cholesterol concentration and low liver cholesterol levels. On the other hand gluten group showed low plasma cholesterol and high liver cholesterol levels, which means body cholesterol pool may not have been changed by the dietary protein. Feeding soy protein meal and the supplemented soy protein isolate resulted in lower plasma cholesterol, plasma triglycerides, liver cholesterol and liver triglycerides levels. This hypolipidemic effect is considered to see unique to soy protein isolate. Rats in gluten and the supplemented gluten groups showed lower plasma protein levels and a tendency of fatty liver.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.9
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• pp.1205-1212
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• 2010

The aim of the present study was to evaluate the effects of replacing 70% of the protein from fish meal by protein from ensiled or dry cassava leaves and sweet potato vines on the performance and carcass characters of growing F1 (Large White${\times}$Mong Cai) pigs in Central Vietnam. Twenty-five crossbred pigs (Large White${\times}$Mong Cai) with an initial weight of 19.7 kg (SD = 0.84) were allocated randomly to five treatment groups with 5 animals per group (3 males and 2 females). Pigs were kept individually in pens ($2.0{\times}0.8\;m$) and fed one of five diets over 90 days. The control diet was formulated with fish meal (FM) as the protein source while the other four diets were formulated by replacing 70% of fish meal protein by protein from ensiled cassava leaves (ECL), dry cassava leaves (DCL), dry sweet potato vines (DSPV) or ensiled sweet potato vines (ESPV). Animals were fed their diets at 4% of BW. Results showed that final BW, ADG, DMI and feed conversion ratio (FCR) among the experimental treatments were not significantly different (p>0.05). ECL or DCL and ESPV reduced feed cost per unit gain by 8-17.5% compared to the fish meal diet. There were no significant differences in carcass characters among the diets (p>0.05). Lean meat percentages and protein deposition ranged 41.5-45.8% and 40.2-52.9 g/d, respectively. Using ensiled or dry cassava leaves and sweet potato vine can replace at least 70% of the protein from fish meal (or 35% of total diet CP) without significant effects on performance and carcass traits of growing (20-65 kg) pigs. Including cassava leaves and sweet potato vines could improve feed cost and therefore has economic benefits.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2001.11a
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• pp.58-60
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• 2001

Two experiments were conducted to study the effects of nutrient level and feeding method of split diets for a.m. and p.m. on laying hen performance. In both studies, ISA Brown layers were kept in laying hen cages and treatments were represented by three or four replicates each containing 25 or 34 birds. In Experiment 1, the control group(C) was fed a conventional single diet throughout the day and split diet groups(T1, T2 and T3) were offered high energy/protein - low Ca diet and low energy/protein - high Ca diets in a.m. and p.m., respectively. In split diet groups, ME and CP consumption, and feed cost required per day and per kg egg mass were significantly reduced(P＜0.05) compared to C group, while the hen-day egg production, average egg weight and daily feed intake were not different among treatments. In the second study, C and T1 groups were fed the diets same to the C and T2 groups in Experiment 1, respectively. And T2 group was fed the diet mixed with the split diets for a.m. in mash and p.m. in pellet used in T1 group. In T1 and T2 groups, daily feed intake and average egg weight were significantly reduced(P＜0.05), while the hen-day egg production was not influenced by the feeding system. Daily ME and CP consumption, and feed cost were reduced(P＜0.05) in T1 and T2, while the ME, CP and feed cost required per kg egg mass were not different to the C group. In both study, eggshell qualities were improved(P＜0.05) by split diet feeding. It was concluded the feed and nutrients consumption, feed cost per day or per kg egg mass could be spared by introducing split diets for a.m. and p.m., and the feeding method of mixed diet of split diets was also convenient and effective for sparing feed cost and improvement of eggshell quality.