• Nutrition Research and Practice
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• v.17 no.4
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• pp.682-697
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• 2023

BACKGROUND/OBJECTIVES: Tibetan tea is a kind of dark tea, due to the inherent complexity of natural products, the chemical composition and beneficial effects of Tibetan tea are not fully understood. The objective of this study was to unravel the composition of Tibetan tea using knowledge-guided multilayer network (KGMN) techniques and explore its potential antioxidant and hypolipidemic mechanisms in mice. MATERIALS/METHODS: The C57BL/6J mice were continuously gavaged with Tibetan tea extract (T group), green tea extract (G group) and ddH2O (H group) for 15 days. The activity of total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) in mice was detected. Transcriptome sequencing technology was used to investigate the molecular mechanisms underlying the antioxidant and lipid-lowering effects of Tibetan tea in mice. Furthermore, the expression levels of liver antioxidant and lipid metabolism related genes in various groups were detected by the real-time quantitative polymerase chain reaction (qPCR) method. RESULTS: The results showed that a total of 42 flavonoids are provisionally annotated in Tibetan tea using KGMN strategies. Tibetan tea significantly reduced body weight gain and increased T-AOC and SOD activities in mice compared with the H group. Based on the results of transcriptome and qPCR, it was confirmed that Tibetan tea could play a key role in antioxidant and lipid lowering by regulating oxidative stress and lipid metabolism related pathways such as insulin resistance, P53 signaling pathway, insulin signaling pathway, fatty acid elongation and fatty acid metabolism. CONCLUSIONS: This study was the first to use computational tools to deeply explore the composition of Tibetan tea and revealed its potential antioxidant and hypolipidemic mechanisms, and it provides new insights into the composition and bioactivity of Tibetan tea.

• Journal of the Korea institute for structural maintenance and inspection
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• v.27 no.2
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• pp.67-76
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• 2023

In this study, in order to enhance the joint capacity between the existing reinforced concrete (R/C) frame and the reinforcement member, we proposed a novel concept of Internal Composite Seismic Strengthening Method (CSSM) for seismic retrofit of existing domestic medium-to-low-rise R/C buildings. The Internal CSSM rehabilitation system is a type of strength-enhancing reinforcement systems, to easily increase the ultimate horizontal shear capacity of R/C structures without seismic details in Korea, which show shear collapse mechanism. Two test specimens of full-size two-story R/C frame were fabricated based on an existing domestic R/C building without seismic details, and then retrofitted by using the proposed CSSM seismic system; therefore, one control test specimen and one test specimen reinforced with the CSSM system were used. Pseudo-dynamic testing was carried out to evaluate seismic strengthening effects, and the seismic response characteristics of the proposed system, in terms of the maximum shear force, response story drift, and seismic damage degree compared with the control specimen (R/C bare frame). Experiment results indicated that the proposed CSSM reinforcement system, internally installed to the existing R/C frame, effectively enhanced the horizontal shear force, resulting in reduced story drift of R/C buildings even under a massive earthquake.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.2B
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• pp.179-189
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• 2010

This paper presents a numerical investigation of vorticity generation in fully vegetated open-channel flows. The Reynolds stress model is used for the turbulence closure. Open-channel flows with rough bed-smooth sidewalls and smooth bed-rough sidewalls are simulated. The computed vectors show that in channel flows with rough bed and rough sidewalls, the free-surface secondary currents become relatively smaller and larger, respectively, compared with that of plain channel flows. Also, open-channel flows over vegetation are simulated. The computed bottom vortex occupies the entire water depth, while the free-surface vortex is reduced. The contours of turbulent anisotropy and Reynolds stress are presented with different density of vegetation. The budget analysis of vorticity equation is carried out to investigate the generation mechanism of secondary currents. The results of the budget analysis show that in plain open-channel flow, the production by anisotropy is important in the vicinity of the wall and free-surface boundaries, and the production by Reynolds stress is important in the region away from the boundaries. However, this rule is not effective in vegetated channel flows. Also, in plain channel flows, the vorticity is generated mainly in the vicinity of the free-surface and the bottom, while in vegetated channel flows, the regions of the bottom and vegetation height are important to generate the vorticity.

• Journal of Korea Entertainment Industry Association
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• v.13 no.8
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• pp.425-434
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• 2019

The purpose of this study is to improve the academy sports field for the establishment of advanced sports culture. This study used qualitative research. The research participants were baseball players for 11 years and have been professional baseball players. Another study participant has been a soccer player for eight years. And he has been a football coach for 13 years. The results of this study are as follows. First, the act of entertaining was the form in which parents served sports leaders meals and alcohol. Parents' student athletes had increased chances of playing in the game and were able to receive faithful guidance. Second, the student athletes whose parents did not act of entertaining to the sports leader were discriminated against. The student athletes were harassed by their leaders, their positions changed, and their chances of playing were reduced. Third, parents' student athletes who did not act of entertaining to their leaders either stopped exercising or moved to another school because of discrimination. Eventually, the entertainment culture of academy sports acted as a mechanism to induce sports de-socialization of student athletes, and made students experience a negative aspect of sports culture. As a result, the entertainment culture of academy sports caused discriminatory treatment due to economic inequality. Therefore, the negative entertainment culture of academy sports should be eradicated for the establishment of advanced sports culture.

• Korean Journal of Social Welfare Studies
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• v.42 no.2
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• pp.381-417
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• 2011

The primary goal of this study is to examine the factors that affect how satisfied sexual minorities are with their lives. This study focuses on how sexual minorities perceive social stigma, the social influences involved in its perception, and the effects this perception has. Using stress-coping theory, this study looks at how social stigma, as a perceived stress, affects social support and self-esteem, how the stigma influences the degree to which individuals "come out" in an effort to cope, and the resultant effects of this coping mechanism on life satisfaction. The data used if from a sample of 478 individuals who self identify as gay, lesbian or bisexual. It was collected through an online survey. Using SEM, the quality was evaluated by analyzing the measurement model, and the relationship of the variables included in the theoretical model was verified by analyzing the structural model. The results of this study show that social stigma directly affects the life satisfaction of Korean sexual minorities. It significantly influences the social support they receive(from family and sexual minority peers), and their self-esteem. The degree to which the individual "comes out" is shown to affect life satisfaction as well. In contrast, the mediating effect of heterosexual support is shown to be insignificant. Based on the analyses, practical strategies regarding social stigma, social support, self-esteem, and "coming out" are suggested for sexual minorities. The social stigma should be reduced and society-scaled campaigns promoted in order to improve their level of life satisfaction. In addition, institutional protection should be developed and specialized educational courses on human rights provided which will empower these minorities with self-help mechanisms. Furthermore, issues such as establishing support system and providing a social welfare system for the sexual minority community are discussed.

• Korean Journal of Culture and Social Issue
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• v.15 no.2
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• pp.251-271
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• 2009

This research measured a concept of mobility which is included objective travel behavior as well as psychological and physical mechanism that influenced travel behavior. And then, this research examined the relation between mobility and the quality of life in elderly drivers. In research 1, to evaluate the reliability and validity of "Mobility Questionnaire(MQ)", we tested "MQ" to 207 drivers aged 20 years and older. In research 2, we examined the mobility level of elderly drivers. Also we investigated the influence that mobility of elderly drivers impacts on their quaility of life. we used "MQ", "Psychological Well-Being Questionnaire(PWBQ)" to 91 drivers aged 65 years and older. To factor analysis, MQ consisted of 'Safe Driving Ability(ADA)', 'Travel Increase(TI)', 'Travel Decrease(TD)', 'Social Activity(SA)', and 'Driving Situational Adaptability(DSA)'. The amount and speed of driving of elderly drivers reduced driving behavior than other drivers. Also, elderly drivers negatively assessed their physical abilities for safe driving and felt a low confidence in specific driving situation. On the other hand, elderly drivers take parts in social activity constructively. A review of the relation of mobility and quality of life showed that mobility of elderly drivers had positive correlation with PWB. Specially, 'ADA', 'SA', and 'DSA' had significant correlations with PWB of elderly drivers. The mobility of elderly drivers had an effect on 'Autonomy', 'Environmental mastery', 'Self-acceptance', and 'Positive relations with others'.

• Biomolecules & Therapeutics
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• v.31 no.6
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• pp.599-610
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• 2023

According to recent evidence, ferroptosis is a major cell death mechanism in the pathogenesis of kidney injury and fibrosis. Despite the renoprotective effects of classical ferroptosis inhibitors, therapeutic approaches targeting kidney ferroptosis remain limited. In this study, we assessed the renoprotective effects of melatonin and zileuton as a novel therapeutic strategy against ferroptosis-mediated kidney injury and fibrosis. First, we identified RSL3-induced ferroptosis in renal tubular epithelial HK-2 and HKC-8 cells. Lipid peroxidation and cell death induced by RSL3 were synergistically mitigated by the combination of melatonin and zileuton. Combination treatment significantly downregulated the expression of ferroptosis-associated proteins, 4-HNE and HO-1, and upregulated the expression of GPX4. The expression levels of p-AKT and p-mTOR also increased, in addition to that of NRF2 in renal tubular epithelial cells. When melatonin (20 mg/kg) and zileuton (20 mg/kg) were administered to a unilateral ureteral obstruction (UUO) mouse model, the combination significantly reduced tubular injury and fibrosis by decreasing the expression of profibrotic markers, such as α-SMA and fibronectin. More importantly, the combination ameliorated the increase in 4-HNE levels and decreased GPX4 expression in UUO mice. Overall, the combination of melatonin and zileuton was found to effectively ameliorate ferroptosis-related kidney injury by upregulating the AKT/mTOR/ NRF2 signaling pathway, suggesting a promising therapeutic strategy for protection against ferroptosis-mediated kidney injury and fibrosis.

• Journal of Life Science
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• v.33 no.11
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• pp.851-858
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• 2023

Unique cartilage matrix-associated protein (UCMA) is an extrahepatic vitamin K-dependent protein rich in γ-carboxylated (Gla) residues. UCMA has been recognized for its ability to promote osteoblast differentiation and enhance bone formation; however, its impact on osteoblasts under hyperglycemic stress remains unknown. In this paper, we investigated the effect of UCMA on MC3T3-E1 osteoblastic cells under hyperglycemic conditions. After exposure to high glucose, the MC3T3-E1 cells were treated with recombinant UCMA proteins. CellROX and MitoSOX staining showed that the production of reactive oxygen species (ROS), which initially increased under high-glucose conditions in MC3T3-E1 cells, decreased after UCMA treatment. Additionally, quantitative polymerase chain reaction revealed increased expression of antioxidant genes, nuclear factor erythroid 2-related factor 2 and superoxide dismutase 1, in the MC3T3-E1 cells exposed to both high glucose and UCMA. UCMA treatment downregulated the expression of heme oxygenase-1, which reduced its translocation from the cytosol to the nucleus. Moreover, the expression of dynamin-related protein 1, a mitochondrial fission marker, was upregulated, and AKT signaling was inhibited after UCMA treatment. Overall, UCMA appears to mitigate ROS production, increase antioxidant gene expression, impact mitochondrial dynamics, and modulate AKT signaling in osteoblasts exposed to high-glucose conditions. This study advances our understanding of the cellular mechanism of UCMA and suggests its potential use as a novel therapeutic agent for bone complications related to metabolic disorders.

• Journal of Life Science
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• v.33 no.10
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• pp.776-782
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• 2023

Silymarin, which is derived from dried Silybum marianum (milk thistle) seeds and fruits, possesses various beneficial properties, such as hepatoprotective, antioxidative, anti-inflammatory, and anticancer activity. This research aimed to explore the antioxidative activity of silymarin against oxidative stress and understand its molecular mechanism in RAW 264.7 cells. The study employed cell viability and reactive oxygen species (ROS) formation assays and western blot analysis. The results demonstrated that silymarin effectively reduced intracellular ROS levels induced by lipopolysaccharide (LPS) in a dose-dependent manner without causing any cytotoxic effects. Moreover, silymarin treatment significantly upregulated the expression of heme oxygenase (HO)-1, a phase II enzyme known for its potent antioxidative activity. Additionally, silymarin treatment significantly induced the expression of nuclear factor-erythroid 2 p45-related factor (Nrf) 2, a transcription factor responsible for regulating antioxidative enzymes, which was consistent with the upregulated HO-1 expression. To investigate the involvement of key signaling pathways in maintaining cellular redox homeostasis against oxidative stress, the phosphorylation status of mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) was estimated by western blot analysis. The results showed that silymarin potently induced HO-1 expression, which was mediated by the phosphorylation of p38 MAPK. To further validate the antioxidative potential of silymarin-induced HO-1 expression, tert-butyl hydroperoxide (t-BHP)-induced oxidative damage was employed and attenuated by silymarin treatment, as identified by a selective inhibitor for each signaling molecule. In conclusion, silymarin robustly enhanced antioxidative activity by inducing HO-1 via the Nrf2/p38 MAPK signaling pathway in RAW 264.7 cells.

• Nutrition Research and Practice
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• v.17 no.6
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• pp.1084-1098
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• 2023

BACKGROUND/OBJECTIVES: Previous research has shown maternal betaine supplementation alleviates fetal-derived hepatic steatosis. Therefore, this study examined the anti-inflammatory effect of maternal betaine intake in offspring mice and its mechanism. MATERIALS/METHODS: Female C57BL/6J mice and their offspring were randomly divided into 3 groups according to the treatment received during gestation and lactation: control diet (CD), fatty liver disease (FLD), and fatty liver disease + 1% betaine (FLD-BET). The FLD group was given a high-fat diet and streptozotocin (HFD + STZ), and the FLD-BET group was treated with HFD + STZ + 1% betaine. After weaning, the offspring mice were given a normal diet for 5 weeks and then dissected to measure the relevant indexes. RESULTS: Compared to the CD group, the offspring mice in the FLD group revealed obvious hepatic steatosis and increased serum levels of alanine aminotransferase, interleukin (IL)-6, and tumor necrosis factor (TNF)-α; maternal betaine supplementation reversed these changes. The hepatic mRNA expression levels of IL-6, IL-18, and Caspase-1 were significantly higher in the FLD group than in the CD group. Maternal betaine supplementation reduced the expression of IL-1β, IL-6, IL-18, and apoptosis-associated speck-like protein containing C-terminal caspase recruitment domain (ASC). Maternal betaine supplementation also reversed the increasing protein expressions of nitric oxide dioxygenase-like receptor family pyrin domain containing 3 (NLRP3), ASC, Caspase-1, IL-1β, and IL-18 in offspring mice exposed to HFD + STZ. Maternal betaine supplementation decreased the homocysteine (Hcy) and s-adenosine homocysteine (SAH) levels significantly in the livers. Furthermore, the hepatic Hcy concentrations showed significant inverse relationships with the mRNA expression of TNF-α, NLRP3, ASC, and IL-18. The hepatic SAH concentration was inversely associated with the IL-1β mRNA expression. CONCLUSIONS: The lipotropic and anti-inflammatory effect of maternal betaine supplementation may be associated with the inhibition of NLRP3 inflammasome in the livers of the offspring mice.