• 한국수산과학회지
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• 제49권5호
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• pp.620-627
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• 2016

A 60-day experiment was conducted to determine the effect of feeding and starvation on the survival, growth, and blood parameters of red sea bream Pagrus major. The starved group was not fed during the first 32 days of the experiment and was then fed for 28 days. The growth rate of the starved fish group was significantly lower than that of the fed fish group. Starvation resulted in growth retardation and reductions in final body weight, growth rate, specific growth rate, and condition factor, whereas the fed fish group grew well and maintained a good condition. The growth rate of the starved group that was refed was higher than that of the fed group. Red blood cells, hematocrit, and hemoglobin showed no significant differences between the fed and starved groups. The cortisol and glucose levels of the fed group of juveniles were higher than those of the starved group. The cortisol levels of the starved group of adults were higher than those of the fed group, whereas the glucose levels of the starved group were lower than those of the fed group. These results suggest that the survival, growth, and hematological parameters used to reflect starvation and feeding in this study provide a useful index of physiological response and survival rate in red sea bream.

• 한국어병학회지
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• 제20권3호
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• pp.211-220
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• 2007

Red sea bream iridovius (RSIV)는 우리나라와 일본의 참돔 (Pagrus major), 돌돔(Oplegnathus fasciatus) 등 주로 돔류에 질병을 유발하는 어류 병원바이러스로 알려져 왔으나, 돔류 이외에도 감수성을 나타내는 어종이 매우 다양하며 아시아를 중심으로 고수온기에 대량 폐사를 유발하는 중요한 원인체로 보고되고 있다. 본 연구는 RSIV에 대한 자연산어류에서 검출동향의 모니터링을 통해 금후 red sea bream iridoviral disease (RSIVD)에 대한 예방학적 접근의 일환으로서 자연산 어류로부터의 RSIV 검출 및 지리적 분포, 보균 어종에 대한 유전학적인 조사를 실시하였다. 조사는 2003년과, 2005년도에 동중국해역 3지점, 서해안 5지점 그리고 남해안 5지점에서 어류를 채집하여 PCR을 이용한 검출결과, 자연산 해산어류 160마리 중 39마리에서 RSIV가 검출되어 24.3%의 높은 검출율을 보였으며 특히 조사된 어류 중 상어목에서 가장 높은 검출율을 보였다. 자연산 어류에서 분리한 RSIV 분리주는 양식산어류에서 분리된 RSIV-K strain과 염기서열 분석에서 97.2%～100%, 아미노산 분석에서 94.7～100% 유사성을 나타내었으며 megalocytivirus Subgroup Ⅲ에 속함을 확인하였다.

• 한국어병학회지
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• 제32권2호
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• pp.49-57
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• 2019

본 연구에서는 2012년부터 2018년 1월까지 국내 어류에서 참돔이리도바이러스병 (red sea bream iridoviral disease; RSIVD) 으로 확정 진단된 주요 분리주에 대하여 major capsid protein (MCP) 유전자의 염기서열을 바탕으로 Megalocytivirus의 유전적 관계를 명확히 했다. 또한, Megalocytivirus와 숙주 세포간 상호작용에 영향을 줄 수 있는 특이 유전자 2종, Vascular endothelial growth factor (VEGF) 및 Histidine triad motif인 HIT-like 단백질 (HIT)에 대한 염기서열 분석을 통해 유전적 상관관계를 고찰하였다. 국내 어류에서 분리된 39개의 megalocytiviruses는 2가지 유전형인 red sea bream iridovirus (RSIV)형과 turbot reddish body iridovirus (TRBIV)형으로 분류되었으며, RSIV형의 megalocytiviruses는 다시 유전아형인 RSIV-subgroup 1형과 2형으로 분류되었다. 그리고 VEGF 유전자 염기서열 분석 결과에서는 RSIV형의 바이러스에서 변이가 발생되었음을 확인할 수 있었으며, HIT-like 단백질 유전자는 RSIV형의 Megalocytivi rus에서만 발견되는 것을 알 수 있었다.

• 한국식품조리과학회지
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• 제16권4호
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• pp.352-357
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• 2000

This study was conducted to investigate the effect of the lipid and collagen content and drip volume on the hardness of fish meat. Red sea bream (cultured and wild) and flounder (cultured, cultured with obosan and wild) were used for this study. Textural differences between cultured and wild meats were determined by the measurements of hardness, lipid and collagen content, and drip volume. Lipid content of the dorsal muscle was higher especially in cultured red sea bream (3.32%) than in wild one. Cultured and wild flounder contained lower content of lipid than red sea bream. The content of collagen was higher in cultured flounder fed with obosan (8.37 mg/g muscle) and wild flounder (8.02 mg/g muscle) than others. Drip volume was the highest in cultured flounder fed with obosan (8.67%). The hardness of raw meat was correlated with the contents of lipid (r= -0.7063) and collagen (r= 0.8307), significantly. Cultured fish contained more lipid and less collagen than wild one. So, the hardness of these fish meats was lower than wild one. However, cultured flounder fed with obosan showed no difference in hardness compared with wild one. In the cooked meat, there was no relationship between the hardness of fish meat and the contents of lipid and collagen. But, the drip volume was significantly related with the hardness (r= 0.6870). From these results, the factors contributing the textural difference between wild and cultured fish meat would be the lipid and collagen contents, and two ways to improve the texture of cultured fish meat could be suggested. One is to lower the lipid content by feed control, and the other is to raise the collagen content by inducing more fish movement.

• 한국식품조리과학회지
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• 제17권5호
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• pp.517-522
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• 2001

Changes of nucleotides and their related compounds in raw, cooked and frozen fish muscle were studied with HPLC. Red sea bream(cultured and wild) and flounder(cultured, cultured with Obosan(equation omitted) and wild) were used for this study. In nucleotides, contents of ATP was similar to that of IMP and some of H$\times$R(inosine) and H$\times$(hypoxanthine) were existed in fresh muscle. ATP was decomposed rapidly and contents of IMP became different between cultured and wild fish after 6 hours. The content of IMP was lower in the cultured red sea bream(3.39$\mu$ mole/g) and flounder(3.17$\mu$ mole/g) than in the wi1d red sea bream(7.31$\mu$ mole/g) and flounder(5.03$\mu$ mole/g). But, the flounder cultured with Obosan contained the largest amounts of IMP After 24 hours, K values of cultured fish muscle(27.7%, 28.2%) were higher than that of wild ones(22.8%, 24.3%). The K value of cultured flounder fed with 0.3% Obosan(equation omitted)(25.7%) was between cultured and wild flounder. IMP was the one which existed the most in cooked and frozen muscle. Amounts of H$\times$R and H$\times$ were more in cooked and frozen muscle. than in raw muscle. From these results, we could suggest that the wild one was more palatable and fresher than the cultured one and the palatability of cultured one seemed to be improved depanding on the feed.

• Fisheries and Aquatic Sciences
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• 제24권11호
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• pp.351-359
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• 2021

The red sea bream iridovirus (RSIV) belonging to genus Megalocytivirus is responsible for red sea bream iridoviral disease (RSIVD) in marine and freshwater fishes. Although several diagnostic assays for RSIV have been developed, diagnostic sensitivity (DSe) and specificity (DSp) of real-time polymerase chain reaction (PCR) assays are not yet evaluated. In this study, we developed a TaqMan probe-based real-time PCR method and evaluated its DSe and DSp. To detect RSIV, the probe and primers were designed based on consensus sequences of the major capsid protein (MCP) genes from megalocytiviruses including RSIV, infectious spleen and kidney necrosis virus (ISKNV), and turbot reddish body iridovirus (TRBIV). The probe and primers were shown to be specific for RSIV, ISKNV, and TRBIV-types megalocytiviruses. A 95% limit of detection (LOD_95%) was determined to be 5.3 viral genome copies/µL of plasmid DNA containing the MCP gene from RSIV. The DSe and DSp of the developed real-time PCR assay for field samples (n = 112) were compared with those of conventional PCR assays and found to be 100% and 95.2%, respectively. The quantitative results for SYBR Green and TaqMan probe-based real-time PCR were not significantly different. The TaqMan probe-based real-time PCR assay for RSIV may be used as an appropriate diagnostic tool for qualitative and quantitative analysis.

• 한국어병학회지
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• 제25권3호
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• pp.263-270
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• 2012

본 연구는 자연산 어류와 양식어류 사이의 병원체 존재에 대한 상관관계구명을 위한 기초 연구의 일환으로서, 우리나라의 자연산과 양식산 넙치, 참돔, 감성돔, 조피볼락의 병원체 감염 현황을 조사하고 그 결과를 비교하였다. 기생충성 질병의 조사 결과, 자연산 넙치와 참돔에서 가장 높게 검출 된 Anisakis sp. (각 58.6%, 41.7%) 는 양식 넙치와 참돔에서 검출되지 않아 자연산과 양식산 어류에서 아주 큰 차이를 보였다. 이는 배합 사료를 공급하는 양식산 어류에 비해, 자연산 어류는 아니사키스에 오염된 갑각류나 작은 물고기 등을 먹이원으로 섭취하기 때문인 것으로 생각되었다. 세균성 질병의 조사 결과, 자연산 어류에 비해 양식산 어류에서 세균이 높은 비율로 검출되었으며, 양식 넙치에서 Vibrio sp.가 18%로 높은 비율로 검출되었다. 비브리오병은 주로 스트레스 등에 의한 2차적 세균성 질병으로 알려져 있는 것으로부터, 양식 넙치가 다른 병원체의 감염, 이동이나 선별, 빈번한 항생제 사용 등 스트레스 환경에 노출된 것으로 판단되었다. 바이러스의 조사 결과, 자연산 어류와 양식어에서 다른 양상을 보였으며, 양식 넙치에서 LCDV (3.9%) 가 가장 높은 검출률을 나타내었다. 본 연구의 결과로부터 자연산과 양식산에서 동시에 검출되는 병원체도 있었으나, 동일 수역에 존재하는 같은 어종이라도, 먹이나 환경에 따라 분리되는 병원체가 다른 것을 확인하였다.

• 한국어병학회지
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• 제25권3호
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• pp.221-229
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• 2012

교잡종, 참돔 및 긴키돔은 수온이 $19.7^{\circ}C$에서 $24.0^{\circ}C$로 상승하는 7월부터 8월에 가장 높은 성장률을 나타낸 반면, 감성돔은 수온이 $23.0^{\circ}C$에서 $19.9^{\circ}C$로 하강하는 9~10월에 가장 높은 성장률을 나타내었다. 생존율은 조사기간 동안 교잡종이 가장 높은 것으로 조사되었지만 이러한 높은 생존율이 잡종 강세의 특성에 의한 것인지에 다른 요인에 의한 결과인지는 추후 자세한 연구가 필요할 것으로 생각된다. 감성돔과 긴키돔에서 녹간증이 관찰되었으나, 교잡종에서 녹간증은 나타내지 않았다. 아가미흡충 감염은 교잡종 0~90%, 참돔 20~100%, 감성돔 10% (10월), 긴키돔 20~100%로 유의적인 차이는 없었지만 교잡종에서 다소 낮은 편이었다.

• 한국식품조리과학회지
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• 제17권6호
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• pp.549-554
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• 2001

Thermal measurements were made for connective tissues of 5 different fish muscles by using a differential scanning calorimeter(DSC), and connective tissues between muscle fibers and the cross sections of muscle fibers were observed by a light microscope. Red sea bream(cultured and wild) and flounder(cultured, cultured with obosan and wild) were used in this study. It was found that the connective tissues of cultured and frozen fish muscle required less endothermic enthalpy and the endothermic peak temperature was lower than those of wild and fresh ones when they were shrunken and denatured. Therefore, it is likely that the former are more unstable to heat than the latter. The cultured flounder fed with obosan and wild flounder which contained more collagen than cultured flounder and the wild red sea bream showed clear connective tissues between fibers. The cross-section of cultured fish muscle fiber was larger than that of wild one. From these results, collagen content and thermal properties of collagen, cross section of muscle fibers seemed to contribute to the textural difference between wild and cultured fish.

• 대한수의학회지
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• 제48권2호
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• pp.175-179
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• 2008

Parallux, a solid-phase fluorescence immunoassay (SPFIA) developed for detection antibiotics residue in milk, was applied for analysis of antibiotics in muscle tissue of olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red sea bream (Pagrus major). Fishes were dipped in neomycin 140 mg/ton water, the recommended therapeutic dose, for 24 h. Muscle samples were obtained on 1st, 2nd, 3rd, 4th and 5th day after drug treatment. The concentration of neomycin in muscle was determined using an internal standard (100 ppb as neomycin). The absorbance ratio of sample to internal standard (S/C) was employed as an index to determine the muscle residues in fishes. To investigate the recovery rate, the standard solutions were added to muscle samples to give final concentrations in muscle of 0.2 and 0.5 mg/ml. The recovery rates of all spiked samples were > 85% of the spiked value. Neomycin was detected in muscles of fishes treated after the 1st day of withdrawal period. On the 2nd day after drug treatment, all muscle samples showed negative reaction (S/C ration ${\leq}$ 1.0). The present study showed that the SPFIA can be applied for predicting residues of neomycin in muscle tissues of farmed fishes.