• Toxicological Research
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• v.23 no.2
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• pp.135-142
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• 2007

Formononetin is an isoflavonoid phytoestrogen found in certain foodstuffs such as soy and red clover. In this study, we examined the action of formononetin with the carcinogen activation pathway mediated through the aryl hydrocarbon receptor (AhR) in MCF-7 breast carcinoma cells. Treating the cells with formononetin alone caused the accumulation of CYP1A1 mRNA as well as elevation in CYP1A1-specific 7-ethoxyresorufin O-deethylase (EROD) activity in a dose dependent manner. However, a concomitant treatment with 7,12-dimethylbenz[a]anthracene (DMBA) and formononetin markedly reduced both the DMBA-inducible EROD activity and CYP1A1 mRNA level. Under the same conditions, formononetin inhibited the DMBA-induced AhR transactivation, as shown by reporter gene analysis using a xenobiotic responsive element (XRE). Additionally, formononetin inhibited both DMBA-inducible nuclear localization of the aryl hydrocarbon receptor (AhR) and metabolic activation of DMBA, as measured by the formation of the DMBA-DNA adducts. Furthermore, formononetin competed with the prototypical AhR ligand, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), for binding to the AhR in an isolated rat cytosol. These results suggest that formononetin might be considered as a natural ligand to bind on AhR and consequently produces a potent protective effect against DMBA-induced genotoxicity. Therefore, that's the potential to act as a chemopreventive agent that is related to its effect on AhR pathway as antagonist/agonist.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.4
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• pp.297-302
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• 1995

The objective of this experiment was to compare the dry matter intake, nutrients digestibility, nitrogen and energy utilization of hehage among the orchardgrass(OG) pure, orchardgrass(OG)-red clover(RC) mixture and complex rnixtures(C. mix) by Korean native goat. The results obtained were surnmerized as follows: 1. Crude protein(CP) content was higher in OG-RC and C. Mix diets than in OG pure diets(P<0.05). NDF content was higher in OG pure diets than other diets(P<0.05). But ADF, Lignin contents and gross energy did not differ among all diets. 2. Dry matter intake by Korean native goat tend to be high in OG-RC diets, and low in OG pure diets. But there was no significant difference statistically. Dry matter and cellular constituents digestibilities were higher for OG-RC and C. Mix diets than OG pure diet, but CP and NDF digestibilities were no significant difference among diets. 3. The amount of nitrogen consumed amount by Korean native goat in the OG-RC diet was higher than other diets. But, due to largely relative urinary and fecal nitrogen loss, Apparrently digested nitrogen and retained nitrogen percents were not significant difference among diets. 4. The amount of energy consumed by Korean native goat in OG-RC diet was higher than other diets. But, due to largely relative urinary and fecal energy loss, digestable energy percent was lower than C. Mix diets. But.metabolic energy percent was no significant difference among diets. Therefore, there was no significant differences among sward types for the utilization of nitrogen and energy by Korean native goats.

• Asian Journal of Turfgrass Science
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• v.26 no.1
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• pp.60-66
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• 2012

Crabgrass (Digitaria sanguinalis L.), Dandelion (Taraxacum platycarpum H. DAHLST.), Annual bluegrass (Poa annua L.), Gree kyllinga (Kyllinga brevifolia var.), Red clover (Trifolium pratense L.), Annual lespedeza (Kummerowia striata (Thunb.)Schindl.), Mugwort (Artemisia capillaris Thunb.), Horseweed (Erigeron canadensis L.), Field horsetail (Equisetum arvense L.) are major weeds in turf. In this study, 23 medicinal plant extracts were tested for herbicidal activities against crabgrass and dandelion in the course of major weed in turf. In pot experiment, we implemented pre-emergence application used medicinal plant extracts showed herbicidal activity in petridish experiment. In effect, inhibition rates of germination were 61.0% at Curcuma longa extract and 62.5% at Cnidium officinale extract in crabgrass pot experiment, and 77.8% at Ailanthus altissima extract in dandelion pot experiment.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.1
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• pp.1-10
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• 1997

Unreduced (2n) gametes are meiotic products (pollen or egg) having a sporophytic (somatic) chromosome number, resulting from abnormalities during either microsporogenesis or megasporogenesis. They occur naturally at a low frequency in many plant species. Unreduced (2n) gametes in plants can be identified for four possible ways as follow i) pollen size and/or shape differences between haploid (n) and diploid (2n) pollen, ii) ploidy analysis (chromosome number) of progeny or meiotic analysis (presence of dyads andlor triads at the microspore stage), iii) progeny performance and fertility and iv) dosage of isozyme and DNA markers. Unreduced (2n) gametes can be an effective breeding tool in synthesizing new cultivars, providing a unique method to maximizing heterozygosity, i.e., transferring a large proportion of the non-additive genetic effects (intra- and inter- locus interactions) h m parent to offspring and can also be used to overcome infertility of interploidy crosses. Sexual polyploidization through 2n gametes has been a major route to the formation of naturally occurring polyploids. The three mechanisms of 2n pollen formation in potato have been discovered as follow: i) parallel spindles (ps) or tripolar spindles (ts), ii) premature cytokinesis (pc-I, pc-2) and iii) synaptic mutants (sy-2, sy-3, sy-4). Genetic analysis indicated that the mechanisms of 2n gamete formation were controlled by single recessive gene in potato, alfalfa, red clover, etc., and by two recessive genes in wheat. The use of 2n gametes which can efficiently transfer germplasm fiom wild relatives to cultivated species, especially fiom diploid to tetraploid could make a contribution to the improvement of germplasm base in breeding programs.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.1
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• pp.32-38
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• 2005

A long-term study initiated in 1989 at San-born Field, Columbia, Missouri, was designed to evaluate the affect of environmental factors, nitrogen application, and crop rotation on soybean (Glycine max [L.] Merr.) seed composition. Soybeans were grown as part of a four- year rotation which included corn (Zea maize L.), wheat (Triticum aestivum L.), and red clover (Trifolium pratense L.). Results from soil tests made prior to initiation of the study and subsequently every five years, were used to calculate application rates of nitrogen, phosphorus, and potassium necessary for target yield of pursuant crops. In the experimental design, nitrogen was applied to one-half of the plot on which the non-leguminous crop, either corn or wheat was grown. Analysis of soybean seed by near infrared reflectance spectroscopy collected over an 11-year period revealed a linear increase in protein and decrease in oil content. Application of nitrogen fertilizer to non-leguminous crops did not have an apparent effect on total protein or oil content of subsequent soybean crop. Analysis of soybean seed proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis in conjunction with computer­assisted densitometry revealed subtle changes in the accumulation of seed proteins. Immunoblot analysis using antibodies raised against the $\beta-subunit$ of $\beta-conglycinin$ showed a gradual increase in the accumulation of the 7S components during successive years of the experiment. A linear increase in temperature and decrease in rainfall was observed from the onset of data· collection. Higher temperatures during the growing season have been linked to increased protein and diminished oil content of soybean, thus changes observed in this study are possibly related to climatic conditions. However, crop rotation and subsequent changes in soil ecology may contribute to these observed changes in the seed composition.

• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.3
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• pp.153-157
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• 1989

This experiment was carried out to investigate the effects of the first harvesting times and regrowth periods after the fist harvest on grass regrowth, dry matter (DM) yield and botanical composition in pasture mixtures during 1987 and 1988. For the test, a split plot design with 3 replications was treated with 3 different first harvesting times (vegetative, stem elongation and heading stage) and 3 different regrowth periods (20, 30 and 40 days) after first harvest. Although the total DM yield of grasses was decreased slightly with earlier cutting, the regrowth DM yield and the regrowth plant height was increased significantly with earlier cutting (p < 0.05). Also, the 30 days of regrowth periods was contributed greatly to the regrowth and the DM yield. The percentage of legumes Tmainly red clover) in botanical composition was increased with later initial harvest anrl longer regrowth period after first harvest. Based on the results, it is suggested that good grass regrowth and seasonal distribution could be achieved by the earlier harvest at fist and the regrowth period of 30 days after first harvest in spring.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.1
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• pp.27-33
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• 1994

This study was conducted to determine the nutritional value of native oriental white oak(Quercus aliena Blume) browse by Korean native goats. Oak browse were mixed with grass hay(orchardgrass 60% and red clover 40%), varing in oak browse content. Diets included 30% oak browse and 70% grass hay, 60% oak browse and 40 % grass hay, and a 100% grass hay(contro1). Oak browse diets were higher in NDF, ADF, lignin, and tannin contents, but lower in CP content than the control diet with increased oak browse levels. Gross energy levels showed no difference between the control and oak browse diets. Dry matter intake was the lowest on diet containing oak browse 60%. Digestibility for DM, cellular constituents, NDF, and ADF showed lower for oak browse diets compared to control diet(P<0.05). Oak browse diets furnished significantly lower dietary N than &d the control diet. Fecal N losses generally increased with higher levels of oak browse levels(P<0.05). Apparently digested N and retained N were lower in oak browse diets than in control diet. The higher retained N was obtained in control diet than in oak browse diets. DE and ME declined linearly as oak browse levels increased when compared to control diet(P

• Archives of Pharmacal Research
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• v.29 no.7
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• pp.570-576
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• 2006

Phytoestrogen biochanin A is an isoflavone derivative isolated from red clover Trifolium pratense with anticarcinogenic properties. This study examined the action of biochanin A with the carcinogen activation pathway that is mediated by the aryl hydrocarbon receptor (AhR) in MCF-7 breast carcinoma cells. Treating the cells with biochanin A alone caused the accumulation of CYP1A1 mRNA and an increase in CYP1A1-specific 7-ethoxyresorufin O-deethylase (EROD) activity in a dose dependent manner. A concomitant treatment with 7,12-dimethylbenz[a]anthracene (DMBA) and biochanin A markedly reduced the DMBA-inducible EROD activity and CYP1A1 mRNA level. In addition, the biochanin A treatment alone activated the DNA-binding capacity of the AhR for the dioxin-response element (DRE) of CYP1A1, as measured by the electrophoretic-mobility shift assay (EMSA). EMSA revealed that biochanin A reduced the level of the DMBA-inducible AhR-DRE binding complex. Furthermore, biochanin A competed with the prototypical AhR ligand, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), for binding to the AhR in an isolated rat cytosol. The biochanin A competitively inhibited the metabolic activation of DMBA, as measured by the formation of the DMBA-DNA adducts. These results suggest that biochanin A may thus be a natural ligand to bind on AhR. Therefore, biochanin A may be due to act an antagonist/agonist of the AhR pathway.

• International Journal of Oral Biology
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• v.44 no.3
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• pp.81-88
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• 2019

Trifolium pratense leaves (red clover) has been used in Oriental and European folk medicine for the treatment of whooping cough, asthma, and eczema, and is now being used to treat and alleviate the symptoms, such as hot flushes, cardiovascular health effects that occur in postmenopausal women. However, relatively little scientific data is available on the physiological activity of this plant. Therefore, in this study, we investigated the anti-cancer activity of T. pratense leaves using methanol extract of T. pratense leaves (MeTP) on human FaDu hypopharyngeal squamous carcinoma cells. MeTP inhibited the viability of FaDu cells by inducing apoptosis through the cleavage of procaspase-3, -7, and -9 and poly (adenosine diphosphate ribose-ribose) polymerase (PARP), downregulation of Bcl-2, and upregulation of Bax, as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Live & dead assay, 4'6-diamidino-2-phenylindole stain, fluorescence-activated cell sorting analysis, and Western blot analysis. In addition, colony formation was slightly inhibited when FaDu cells were treated with a non-cytotoxic concentration (0.125 mg/mL) of MeTP and almost completely inhibited when cells were treated with 0.25 mg/mL MeTP. Collectively, these results indicate that MeTP induced cell apoptosis via caspase- and mitochondrial-dependent apoptotic pathways, and inhibited colony formation of cancer cells in FaDu human hypopharyngeal squamous carcinoma cells. These findings suggest MeTP should be considered for clinical development as a chemotherapeutic option in oral cancer.

• Journal of Internet Computing and Services
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• v.5 no.5
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• pp.1-15
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• 2004

In this paper, we explain the design and implementation of GWB(Gene WorkBench), which is a web-based, integrated system for efficiently managing and analyzing genomic sequences, Most existing software systems handling genomic sequences rarely provide both managing facilities and analyzing facilities. The analysis programs also tend to be unit programs that include just single or some part of the required functions. Moreover, these programs are widely distributed over Internet and require different execution environments. As lots of manual and conversion works are required for using these programs together, many life science researchers suffer great inconveniences. in order to overcome the problems of existing systems and provide a more convenient one for helping genomic researches in effective ways, this paper integrates both managing facilities and analyzing facilities into a single system called GWB. Most important issues regarding the design of GWB are how to integrate many different analysis programs into a single software system, and how to provide data or databases of different formats required to run these programs. In order to address these issues, GWB integrates different analysis programs byusing common input/output interfaces called wrappers, suggests a common format of genomic sequence data, organizes local databases consisting of a relational database and an indexed sequential file, and provides facilities for converting data among several well-known different formats and exporting local databases into XML files.