• Biomolecules & Therapeutics
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• v.26 no.5
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• pp.474-480
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• 2018

Most angiogenesis assays are performed using endothelial cells. However, blood vessels are composed of two cell types: endothelial cells and pericytes. Thus, co-culture of two vascular cells should be employed to evaluate angiogenic properties. Here, we developed an in vitro 3-dimensional angiogenesis assay system using spheroids formed by two human vascular precursors: endothelial colony forming cells (ECFCs) and mesenchymal stem cells (MSCs). ECFCs, MSCs, or ECFCs+MSCs were cultured to form spheroids. Sprout formation from each spheroid was observed for 24 h by real-time cell recorder. Sprout number and length were higher in ECFC+MSC spheroids than ECFC-only spheroids. No sprouts were observed in MSC-only spheroids. Sprout formation by ECFC spheroids was increased by treatment with vascular endothelial growth factor (VEGF) or combination of VEGF and fibroblast growth factor-2 (FGF-2). Interestingly, there was no further increase in sprout formation by ECFC+MSC spheroids in response to VEGF or VEGF+FGF-2, suggesting that MSCs stimulate sprout formation by ECFCs. Immuno-fluorescent labeling technique revealed that MSCs surrounded ECFC-mediated sprout structures. We tested vatalanib, VEGF inhibitor, using ECFC and ECFC+MSC spheroids. Vatalanib significantly inhibited sprout formation in both spheroids. Of note, the $IC_{50}$ of vatalanib in ECFC+MSC spheroids at 24 h was $4.0{\pm}0.40{\mu}M$, which are more correlated with the data of previous animal studies when compared with ECFC spheroids ($0.2{\pm}0.03{\mu}M$). These results suggest that ECFC+MSC spheroids generate physiologically relevant sprout structures composed of two types of vascular cells, and will be an effective pre-clinical in vitro assay model to evaluate pro- or anti-angiogenic property.

• Korean Journal of Veterinary Research
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• v.52 no.3
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• pp.163-167
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• 2012

Mosapride stimulated dietary motility was introduced because of the arrhythmogenic effect of cisapride. Cisapride, 5-HT receptor agonist, induces prolongation of QT interval. Additionally, this condition can raise the possibility of acute, "malignant" arrhythmias such as torsade de pointes. It is hard to find any reports about effects of mosapride on cardiac parameters in dogs. By confirming electrocardiogram (ECG) parameters, the surface extremity leads ECG that was obtained from the four-limb electrodes and which was recorded by an ECG recorder after administration of mosapride 3 mg/kg PO b.i.d, and mosapride 3 mg/kg with itraconazole 5 mg/kg PO b.i.d, respectively. QT interval was shortened on the days of 3, 5, and post-day 1 in both mosapride 3 mg/kg administrated group and mosapride with itraconazole group. Heart rate increased significantly. QTc was slightly prolonged in mosapride administration group and mosapride with itraconazole group. However, all dogs of QTc were in normal variation (150~250 msec). Besides, the dogs showed no side effects reported in human medicine during the administration with these drugs. Although mosapride can increase the heart rate, this study suggest that mosapride may be useful for the dogs with disorders of gastrointestinal motility because of no fatal arrhythmogenic effect inspite of administration with itraconazole in dogs.

• Journal of Chest Surgery
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• v.14 no.3
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• pp.228-234
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• 1981

Measurement of cardiac output by thermodilution method is easy to perform and can be repeated at very short intervals and offers a number of theoretical and practical advantages. The purpose of this paper is to reevaluate the accuracy and reproducibility of the thermodilution cardiac output by comparing the results with those obtained by the direct Fick technique. From January through April 1981 [4 months], 26 simultaneous cardiac output measurements were made using thermodilution and direct Fick methods in 12 patients in the intensive care unit of Severance Hospital following open heart surgery. The average age of the patients was $34.8{\pm}12.0$ [SD] years [range; 17-57 years]. For cardiac output determination by thermodilution technique [$CO_TD$], a Swan-Ganz Thermodilution Catheter, Model 93A-131-7F [Edwards Laboratories], a Cardiac Output Computer, Model 9520A, and Strip Chart Recorder, Model 9810 [Edwards Laboratories] were used. For the determination of cardiac output by direct Fick principle [$CO_Fick$], a Van Slyke Manometric Apparatus [Thomas] for the measurements of arteriovenous oxygen content difference and a Collin`s Respirometer with Kymograph for the measurement of oxygen consumption were used. The correlation between the simultaneously measured $CO_TD$ and $CO_Fick$ was found to be $CO_Fick$ = 0.95.$CO_TD$ + 0.1073, [r= 0.9777, P < 0.001 ]. For 26 double or triple determinations of $CO_TD$, the values ranged from 1.77 L/min to 6.66 L/min, with a reproducibility of 2.18%. The maximum difference of $CO_TD$ for $CO_Fick$ was between +11.99% and -12,99% [$Mean{\pm}SD$ = $6.07{\pm}3.97$%]. Measurement of cardiac output by thermodilution method provides a simple, rapid, reproducible and highly accurate method for multiple cardiac output measurements suitable for use at the bedside. Comparison of Cardiac Output Determination by Direct Fick and Thermodilution Method in Man.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.16 no.2
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• pp.183-192
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• 2006

Predicting energy expenditure (EE) is important to prevent work-related musculoskeletal disorders (WMSDs). The problem to predict EE is that the standard of EE is based on western data. The authors checked average EE by job categories to provide basic data for suggesting proper work intensity for Korean workers. This study was conducted from 2003 to 2005. Study subjects were recruited from 4 car parts assembly plant, 2 car assembly plant, 2 Heavy machine manufacturing plant and 2 shipyards. Total study subjects were 515 male workers. To estimate VO2max, sub-maximal test was conducted to measure VO275%max by bicycle ergometer (Combi Co, Aerobike 75XL II). Heartbeats were recorded with heartbeat recorder (Polar Electro Co, Finland, S810) during work. EE of work was calculated by recorded heartbeat and individual regression equation which was derived from sub-maximal test. Subjects were classified into 4 industry and 8 work posture, 23 job task categories. Mean EEs (S.D.) according to industry classification (kcal/min) were 4.9 (0.7), 4.8 (0.7), 4.9 (0.7), 5.0 (0.9), and 4.0 (0.5) for Car Part manufacture, Car Assembly, Ship Building, Heavy Machinery Manufacture, and Hospital Office, respectively. The results suggest that Korean male workers of exceeding to the NIOSH criteria will be needed to plan for job rescheduling to maintain $worker^{\circ}$Øs health. Further study to establish Korean work intensity standard would be needed.

• Journal of Biosystems Engineering
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• v.39 no.1
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• pp.34-38
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• 2014

Purpose: In this study, the performance of cooling system with the water-water heat pump system of 100kW scale made for cooling agricultural facilities, especially for horticultural facilities, was analyzed. It was intended to suggest performance criteria and performance improvement for the effective cooling system. Methods: The measuring instruments consisted of two flow meters, a power meter and thermocouples. An ultrasonic and a magnetic flow meter measured the flow rate of the water, which was equivalent to heat transfer fluid. The power meter measured electric power in kW consumed by the heat pump system. T-type thermocouples measured the temperature of each part of the heat pump system. All of measuring instruments were connected to the recorder to store all the data. Results: When the water temperature supplied into the evaporator of the heat pump system was over $20^{\circ}C$, the cooling Coefficient Of Performance(COP) of the system was higher than 3.0. As the water temperature supplied into the evaporator, gradually, lowered, the cooling COP, also, decreased, linearly. Especially, when the water temperature supplied into the evaporator was lower than $15^{\circ}C$, the cooling COP was lower below 2.5. Conclusions: In order to maintain the cooling COP higher than 3.0, we suggest that the water temperature supplied into evaporator from the thermal storage tank should be maintained above $20^{\circ}C$. Also, stratification in the thermal storage tank should be formed well and the circulating pumps and the pipe lines should be arranged in order for the relative low-temperature water to be stored in the lower part of the thermal storage tank.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.16 no.1
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• pp.1-15
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• 1980

An underwater recording system was designed to measure the sound spectra of the underwater noises produced by fishing gears in operation. Recorded were noi~es from three types of fishing gears: an anchovy set net, three anchovy boat seine net and a stern trawlnet. Acoustic analysis were made using a heterodyne analyzer, a digital frequency analyzer and a level recorder. The no;'e produced by the anchovy set net was found in the high frequency region of the onset of ambient noise spectrum with a slope of - 6 dB/octave. Here the ambient noise spectrum is higher, though similar in shape, than Knudsen spectrum, and is attributed to the breaking action of the coastal wave. Measured noise spectra during the fishing operations of the anchovy boat seine nets are attributed to the background noise of the sea in the presence of the fishing vessels. The frequency distribution of the noise was 20~5, 000 Hz in the case of two steel anchovy boat seiners, and 20-3,000 Hz in the case of the wooden anchovy boat seiner. The predominant frequency range was 250~350 Hz and maximum sound pressure level was 122 dB (re $1\muPa$) in the case of the steel boat and ] 17 dB in the case of the wooden boat. The noises produced by the trawl fishing gears are remarkably higher than the background noi~e in the presence of the fishing vessel. The frequency distribution of the noi~e was 20-6,300 Hz. The predominant frequency range was 100~200 Hz and maximum sound pressure level was 137 dB ( re $1\muPa$) . The noise spectra were not so much different from that caused by vibrations of the towing cable and the structure of the ground rope of the trawl net towed in an experimental tank.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.26 no.2
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• pp.104-111
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• 2015

Background and Objectives : Acoustic analysis of voice could be influenced so much by the quality of voice files which were recorded by recording device. In clinical practice, voice files that were recorded by analysis program directly or portable digital recording device were analyzed mostly. This study examined the feasibility of using Galaxy smartphone recordings for acoustic analysis of voice. Materials and Methods : Acoustic measures were compared between voice signals recorded from 30 normal speakers (15 males and 15 females) through Galaxy smartphone, portable digital recording device and CSL. Fo, jitter, shimmer, NHR (Noise-Harmony ratio) and Formant frequencies were analyzed by MDVP. Results : Fo, Jitter, Shimmer, NHR and formant frequencies from 3 devices were no significantly difference. The intraclass correlation coefficient (ICC) was higher between each of the voice perturbation measures. Conclusion : The findings indicated that Galaxy smartphone recording system was useful device for acoustic analysis of voice. Furthermore, Galaxy smartphone can be applied widely in various way for acoustic analysis of voice.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.14 no.4
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• pp.260-264
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• 1981

The purpose of the present study is to measure the sound spectrum of the underwater noise generated by a stern trawler M/S Saebada (2,275 GT, 3600 ps) in the various operational conditions. Underwater noises were recorded by a hydrophone (B & K 8100) and analyses were made rising a digital frequency analyzer (B & K 2131) and level recorder (B & K 2370). The predominant frequency range was 100-500 Hz, and maximum sound pressure level was 121 dB(re. $1{\mu}Pa$). Underwater noise level increased with the increased speed of the vessel. Sound pressure level measured in the course of astern cruising was higher than that measured in the course of ahead cruising and also the noise spectrums were different in these two cases. At the time of cruising the underwater noise was higher than 10 dB compared to those values measured at the time at rest with only engine operation. The underwater noise of the vessel was mainly due to the main engine revolution of the propeller and the vibration of hull.

• Aerospace Engineering and Technology
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• v.3 no.1
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• pp.170-178
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• 2004

This paper deals with six degree-of-freedom HILS(hardware-in-the-loop-simulation) of KSR-III rocket using a TAFMS(three axis flight motion simulator). This TAFMS HILS test is accomplished before main HILS tests in order to verify the control stability in the presence of TAFMS dynamic effects. The TAFMS HILS test includes initial attitude holding tests for INS initial alignment procedures, timer synchronization tests with an auxiliary lift-off signal, real-time calibration tests using an external thermal recorder, open-loop TAFMS operating tests, and final closed-loop TAFMS HILS tests using the TAFMS attitude measurements as inputs to the closed control loop. The HILS tests are accomplished for several flight conditions composed with nominal flight condition, TWD effect added condition, slosh modes and/or bending modes existing condition, and windy condition, etc.

• Physical Therapy Rehabilitation Science
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• v.6 no.2
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• pp.65-70
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• 2017

Objective: The purpose of this study was to examine the intertester reliability and validity of four nonradiologic measurements of thoracic spine rotation in healthy adults. Design: Descriptive laboratory study. Methods: This study was conducted on 20 male and 20 female university students aged between 19 and 26. To measure thoracic rotation, a goniometer, a bubble inclinometer, a dual inclinometer, and a smartphone application-clinometer were used. The measurement was performed twice for each device and the same measurement was performed by two examiners. The measurements were performed in the lumbar locked position. The arm in the direction of rotation was taken back and placed onto the back of the lumbar region. With right and left trunk rotation, the head was rotated together but remained in the center line so that the axial rotation was maintained. Both examiners performed the measuring procedures and directly handled the measuring instrument. All measurement results were recorded by the recorder. Results: The range of motion (ROM) of thoracic rotation in lumbar locked position for all four devices was 47 degrees. The intra-rater reliability estimates ranged from 0.738 to 0.906 (p<0.05). The inter-rater reliability estimates ranged from 0.736 to 0.853 (p<0.05). The goniometer, bubble inclinometer, dual inclinometer, and smartphone clinometer showed high validity (p<0.05). This result indicates that all four devices may be used by the same examiner and by other examiners obtaining follow-up measurement. Conclusions: The use of the goniometer, bubble inclinometer, dual inclinometer, and smartphone clinometer for measurements in the lumbar locked posture are reliable and valid nonradiologic measures of thoracic rotational ROM in healthy adults.