• The Journal of the Korea Contents Association
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• v.18 no.4
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• pp.254-267
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• 2018

The purpose of this study was to look into psychological mechanisms about the appellation of the stepparent by children of reconstituted(cohabited) family after parents' divorce in the child's perspective. In-depth interview was used for the qualitative research method. Research participants four children from reconstituted(cohabitated) families after their parents' divorces. According to the data analysis, the reason children hesitate to call their stepparents father or mother is that 'why do I need to call them my father/mother when I have my own?' 'why do I need them when I have been living just fine without them?' 'I don't call them father/mother because it is just uncomfortable!' Based on such research results, counseling guidelines were provided to arbitrate conflicts about appellations stepparent by children of reconstituted (cohabited) family after parents' divorce.

• Journal of the Korea Safety Management & Science
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• v.16 no.2
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• pp.101-109
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• 2014

This study is about safety education experience and practice of parents who are raising preschool children and the study results are as the following. For safety education experience state, parents had experience of safety education and mostly received it through communities, TV, Internet, etc. The most common educational content was the traffic safety education. For safety education practice degree according to number of children, safety education practice was mostly done well with 1~2 children. For safety education practice degree according to family type, safety education practice degree within the family was done most in nuclear families while healthy life habit safety education practice degree was done most in nuclear families and reconstituted families. Also, for traffic safety education practice degree, it was done well in nuclear families. For respiratory disease safety education practice degree, nuclear families and reconstituted families were the highest and for first aid safety education practice degree, single-parent families were the highest. For safety knowledge level of parents, safety knowledge of family was the highest, followed by safety knowledge of respiratory disease, and traffic. For safety education practice degree and safety knowledge according to safety education experience of parents, there was a significant difference in practice degree according to safety education experience of parents and there was also difference in practice knowledge according to experience. There was also education effect in healthy life habit safety knowledge and traffic safety knowledge.

• Journal of Families and Better Life
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• v.21 no.1
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• pp.25-36
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• 2003

This study was conducted to reconstitute a family life education program for caregivers and to analyze the system of implementation. Based largely on a pre-existing program for caregiving families, I developed a new educational program. Personal time management and personal needs management were added in the content of the program order to help enhance self-esteem and internal control. The reconstituted educational program, which consists of 6 sessions, was implemented on a small group of caregivers in Jeollabukdo Province who support their elderly parents. The effectiveness of the program was evaluated by a pre-test, a post-test, a follow-up test and open-questionnaire reports. The results from the post-test suggest that the program has been effective in improving the knowledge on aging process, internal control and self-esteem. But the follow-up test results show the program has been effective only in improving the knowledge on aging process. According to the open-questionnaire reports, the program seems to have been effective in improving interpersonal relationships and changing attitudes toward their elderly parents.

• Development and Reproduction
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• v.17 no.1
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• pp.9-16
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• 2013

Coactivator-associated arginine methyltransferase 1 (CARM1) is included in the protein arginine methyltransferase (PRMT) family, which methylates histone arginine residues through posttranslational modification. It has been proposed that CARM1 may up-regulate the expression of pluripotency-related genes through the alteration of the chromatin structure. Mouse embryonic stem cells (mESCs) are pluripotent and have the ability to self-renew. The cells are mainly used to study the genetic function of novel genes, because the cells facilitate the transmission of the manipulated genes into target mice. Since the up-regulated methylation levels of histone arginine residue lead to the maintenance of pluripotency in embryos and stem cells, it may be suggested that CARM1 overexpressing mESCs elevate the expression of pluripotency-related genes in reconstituted embryos for transgenic mice and may resist the differentiation into trophectoderm (TE). We constructed a fusion protein by connecting CARM1 and 7X-arginine (R7). As a cell-penetrating peptide (CPP), can translocate CARM1 protein into mESCs. CPP-CARM1 protein was detected in the nuclei of the mESCs after a treatment of 24 hours. Accordingly, the expression of pluripotency-related genes was up-regulated in CPP-CARM1-treated mESCs. In addition, CPP-CARM1-treated mESC-derived embryoid bodies (EBs) showed an elevated expression of pluripotency-related genes and delayed spontaneous differentiation. This result suggests that the treatment of recombinant CPP-CARM1 protein elevates the expression of pluripotency-related genes of mESCs by epigenetic modification, and this protein-delivery system could be used to modify embryonic fate in reconstituted embryos with mESCs.

• Journal of Life Science
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• v.22 no.11
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• pp.1456-1462
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• 2012

Sorafenib is a multikinase inhibitor and an oral anticancer drug approved for the treatment of patients with advanced renal cell carcinoma and those with unresectable hepatocellular carcinoma. The purpose of this study was to develop an efficient method of the determination of sorafenib in human plasma using tandem mass spectrometry coupled with liquid chromatography (LC/MS/MS) and validate the method by the guidelines of the Korean Food and Drug Administration (KFDA). Plasma samples ($100{\mu}l$) were added with chlorantraniliprole as an internal standard and then mixed with the 0.1% formic acid-containing extraction solution composed of isopropyl alcohol and ethyl acetate (1:4, v/v). After centrifugation, the supernatant was concentrated at $45^{\circ}C$ under negative pressure and centrifugal force. The residue was reconstituted with a mobile phase and injected into the HPLC instrument using a reverse phase Waters XTerra$^{TM}$ C18 column (particle size $3.5{\mu}m$). Liquid chromatography was carried out within the run time of 5 min using a mobile phase composed of buffer (0.1% formic acid and 10 mM ammonium formate), methanol, and acetonitrile (1:6:3, v/v/v). The analytes were monitored by tandem mass spectrometry in the multiple reaction monitoring method programmed to detect sorafenib at 'm/z 465.2 ${\rightarrow}$ 252.5' and chlorantraniliprole at 'm/z 484.4 ${\rightarrow}$ 286.2' with positive electrospray ionization mode ($ES^+$). The result showed the proper linearity ($r^2$ > 0.99) over the range of 2,000-5,000 ng/ml with good accuracy (90.7-103.9%) and precision (less than 10%). The newly developed method using LC/MS/MS was validated by the guideline of KFDA and identified as more sensitive compared to the previous methods.