• 대한미생물학회지
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• 제21권2호
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• pp.243-249
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• 1986

A study of the immunogenicity and reactogenicity of two doses of lot H(10, 20 mcg), two doses of lot L (20, 40 mcg) of the Smith Kline-RIT recombinant DNA yeast-derived hepatitis B vaccine and a 20-mcg dose of the Merck Sharp and Dohme plasma-derived hepatitis B vaccine was conducted in young adults under randomized, double-blind conditions. Immunization was carried out according to a 0-, 1-, and 6-month vaccination schedule. Results indicated that the yeast-derived hepatitis B vaccine was well tolerated and immunogenic. Reactogenicity to both yeast- and plasma-derived vaccines was mild in severity and low in incidence with no significant differences appearing between the study groups. One month after the third dose, the yeast-derived vaccines induced a high degree of soroconversion ranging between 95.0% and 100%. The response was not lot or dose-dependent. The administration of the plasma-derived vaccine resulted in anti-HBs geometric mean titres statistically signifirantly higher than those elicited by the different yeast-derived hepatitis B vaccines one month after the third dose of vaccine but the difference was not large enough to be of great clinical significance.

• 한국독성학회:학술대회논문집
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• 한국독성학회 1997년도 국제심포지움 및 추계학술대회
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• pp.38-42
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• 1997

인간성장 호르몬은 191개의 아미노산으로 구성된 펩타이드 호르몬의 일종으로 사람의 뇌하수체에서 분비된다. 본연구의 목적은 저렴한 가격의 재조합 성장호르몬을 안정적으로 공급함은 물론 부작용을 최대한으로 줄이는데 있다. 효모에서의 성장호르몬 재조합 과정은 유전자조작과 이에따른 정제기술의 확립을 시작으로 물리 화학적 또는 생물학적 물성 연구, 다양한 전임상 시험 및 임상 시험의 단계를 수행한 후, 인허가 과정을 거쳐서 완료되었다. 다양한 실험 결과에 의하면 당소에서 재조합 기술로 생산한 고순도의 인간성장 호르몬은 안정된 물성을 보유하고 있을 뿐만아니라 전임상 시험 결과 인체 투여용량의 수십배의 고용량을 투여하여도 별다른 독성 증상을 관찰할 수 없었으며, 임상 시험에서도 대상 환아의 신장이 지속적으로 성장하는 등 만족할 만한 결과를 보여 주었다.

• Journal of Microbiology and Biotechnology
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• 제1권3호
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• pp.157-162
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• 1991

Conditions for glutathione production in E. coli cells which possess pGH501 (2 gshI+gshII) were studied. In terms of ATP supply for the glutathione synthesis, two different systems have been constructed and compared. When the acetate kinase reaction of E. coli was used for ATP generation, 20 mM of L-cysteine was completely converted to glutathione by toluene-treated E. coli cells (100 mg/ml) harboring pGH501 within 2 h at $37^{\circ}C$. However, considering the economical aspects, the glycolytic pathway of yeast was chosen as a better system for ATP generation. The optimal concentrations of reactants for glutathione production were determined to be as follows; 80 mM L-glutamate, 20 mM L-cysteine, 20 mM glycine, 20 mM $MgCl_2$, 50 mM potassium phosphate buffer (pH 7.5), 400 mM glucose, polyoxyethylene stearylamine ($5\;\mul/ml$), toluene-treated E. coli HB101/pGH501 (100 mg/ml), and dried yeast cells (400 mg/ml). The conversion ratio of L-cysteine to glutathione was 80% (about 5 mg/ml) under optimal condition within 6 h at $37^{\circ}C$.