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Growth differentiation factor 9 and cumulus cell supplementation in in vitro maturation culture media enhances the viability of human blastocysts

  • Chatroudi, Mahla Honari;Khalili, Mohammad Ali;Ashourzadeh, Sareh;Anbari, Fatemeh;Shahedi, Abbas;Safari, Somayyeh
    • Clinical and Experimental Reproductive Medicine
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    • v.46 no.4
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    • pp.166-172
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    • 2019
  • Objective: In vitro maturation (IVM) of immature oocytes can be useful for some infertile patients. In IVM programs, the rates of embryo formation and pregnancy are low. Therefore, it is essential to recognize the main factors involved in regulating oocyte maturation in vitro. The purpose of this study was to investigate the effects of growth differentiation factor 9 (GDF9) and cumulus cell (CC) supplementation in IVM medium on the rates of embryo formation and viability of human blastocysts. Methods: A total of 80 germinal vesicle oocytes from stimulated cycles underwent an IVM program. The oocytes were divided into four groups, where group I consisted of IVM media only and served as the control, group II consisted of IVM+CCs, group III consisted of IVM+GDF9 (200 ng/mL), and group IV consisted of IVM+CCs+GDF9 (200 ng/mL). Intracytoplasmic sperm injection was performed on the IVM oocytes, and the cleavage embryos that were generated were vitrified. Following thawing, the embryos were cultured for 3 additional days, and the viability rates of the developed blastocysts were determined. Results: The maturation rate of the oocytes did not differ significantly across the four groups. The fertilization rate in group II was significantly higher than that in the control group (76.5% vs. 46.2%). Embryo formation was significantly more frequent in all experimental groups than in the control group, while blastocyst formation did not show significant differences in the three experimental groups compared to the control. The mean viability rates in groups II, III, and IV were 58.16%, 55.91%, and 55.95%, respectively, versus 37.78% in the control group (p< 0.05). Conclusion: Supplementation of IVM culture media with GDF9 and CCs enhanced the fertilization, embryo formation, and viability rates of blastocysts generated from vitrified cleavage embryos.

A Study on the Types and Usages of PDA Users (PDA사용자의 유형 및 사용방법에 관한 연구)

  • Shon Young-Woo;Kim Jeong-Han
    • Journal of Digital Contents Society
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    • v.3 no.2
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    • pp.211-220
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    • 2002
  • It has not been long since the current form of PDA began to be used widely. The PDA, which first came out in a recognizable form in the market a decade ago, multiplying the number of users, has recently been developed in various forms. It began to make cutting-edge technology available in PDA, and to be subdivided into various forms appropriate for special purposes, which reflected the recent intense competition in the market. It included the function of mobile phone, in tandem with the original function of PDA, by which it brought about other synergy effects and corresponded with the purpose more solidly. Due to these recent changes, PDA has had a dramatically increasing number of users, as well as the wider scope of usage and practical application by introducing new technology. However, research is required in the fields of basic usage and interface. It is important to recognize that PDA is an apparatus that stores for the users contents and information suitable for them, and that responds to them in case of need. PDA therefore needs to convey what the users require more easily and speedily without errors, and to provide them with appropriate information and contents. This paper examined the extent to which the interface of PDA including the input of contents required by the user would be efficient by questionnaires. In addition, alternatives concerning a general application environment of PDA were suggested based on the result of this survey.

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Electronic-Composit Consumer Sentiment Index(CCSI) development by Social Bigdata Analysis (소셜빅데이터를 이용한 온라인 소비자감성지수(e-CCSI) 개발)

  • Kim, Yoosin;Hong, Sung-Gwan;Kang, Hee-Joo;Jeong, Seung-Ryul
    • Journal of Internet Computing and Services
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    • v.18 no.4
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    • pp.121-131
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    • 2017
  • With emergence of Internet, social media, and mobile service, the consumers have actively presented their opinions and sentiment, and then it is spreading out real time as well. The user-generated text data on the Internet and social media is not only the communication text among the users but also the valuable resource to be analyzed for knowing the users' intent and sentiment. In special, economic participants have strongly asked that the social big data and its' analytics supports to recognize and forecast the economic trend in future. In this regard, the governments and the businesses are trying to apply the social big data into making the social and economic solutions. Therefore, this study aims to reveal the capability of social big data analysis for the economic use. The research proposed a social big data analysis model and an online consumer sentiment index. To test the model and index, the researchers developed an economic survey ontology, defined a sentiment dictionary for sentiment analysis, conducted classification and sentiment analysis, and calculated the online consumer sentiment index. In addition, the online consumer sentiment index was compared and validated with the composite consumer survey index of the Bank of Korea.

From 'Medicalization' to 'Biomedicalization': the Case of Mental Disorder ('의료화'에서 '생의료화'로: 정신장애의 사례)

  • Kim, Hwan-Suk
    • Journal of Science and Technology Studies
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    • v.14 no.1
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    • pp.3-33
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    • 2014
  • Over the last forty years, the dominant perspective of social science on medicine has been the medicalization theory. It indicates the social process of expanding power of medical professionals by (re)defining the problems which were treated as non-medical phenomena(e.g. homosexuality, alcoholism, obesity, etc.) into "diseases" and thus the spheres of medical intervention. Meanwhile, rapid technoscientific changes in the medical field owing to the diffusion of biological sciences and information technologies since the mid-1980s and the accompanying emergence of new social arrangements such as bioeconomy and biological citizenship have led to the rise of a new social scientific perspective called the biomedicalization theory. This paper attempts to compare the two theories and assess their merits and demerits as a basic work to deepen the understandings of sociology and STS on contemporary medicine. And it also attempts to analyze their relative relevance through the case of mental disorder. The analysis on the case of mental disorder clearly shows that the medicalization in that area seems to have continuously proceeded since the early 19th centiry to the present. Furthermore, it also seems true that the five central processes of biomedicalization(except for risk surveillance technologies of mental disorder) have been observed and realized since the late 20th century. These results indicate that although medicalization has consistently proceeded, it has not been limited to the quantitative expansion of the medical field but been extended to the qualitative transformation asserted by the biomedicalization theory. Therefore, while the concept of medicalization is valid and significant even today, we can recognize that the concept of biomedicalization allow us to capture the new phenomena which cannot be properly and sufficiently captured by that of medicalization.

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A Phenomenological Study for the Inquiry into Long Life Factors in Persons of One Hundred and Above on Cheju Island (제주도 100세이상 노인의 장수요인 규명을 위한 현상학적 연구)

  • Kim, Seong-Hyuk
    • Research in Community and Public Health Nursing
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    • v.9 no.1
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    • pp.40-63
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    • 1998
  • A long life is the desire of many people. The purpose of this study was to describe long life factors for people aged one hundred and above on Chju island. The research was conducted using a phenomenological method to add understanding to this subject. The field work for this study was done from July, 1997 to February, 1998 on Cheju island. The sample consisted of 10 persons of one hundred or above and 12 families. They were audio-recorded and analyzed using Colaizzi's (1978) method. The results of the present study shows the following: The thoughts of the persons one hundred and above showed ten important concepts; 'dietary regimen', 'mental regimen', 'sleep regimen', 'residence regimen', 'health regimen', 'labor regimen', 'exercise regimen', 'being born', 'filial piety and posterity', 'attachment to life'. Concerning 'Dietary Regimen': Dietary regulation, Preferential food intake, Living on vegetables, Light eating, and concern for longevity in food intake were important. Concerning 'Mental Regimen' : Harmony, Clearheadedness, Mildness, Abstinence, Generosity, Relaxation, Gaiety, Inspiration and Strength were important. Concerning 'Sleep Regimen'; Enough sleeping hours, Sound sleep, Curved sleeping posture, and Comfortable bedding were important. Concerning 'Residence Regimen' ; Clean dwelling, Pleasant surroundings, Thatched house life, Living in the village with good-hearted people, and Regular life were important. Concerning 'Health Regimen'; Temperance of drug, use Proper temperature, Proper humidity, and a Clean body were important. Concerning 'Labor Regimen'; Agricultural labor, Diligence, and Domestic labor were important. Concerning Exercise Regimen': Proper exercise, and Sunbathing were important. Concerning 'Being Born' ; Strong physical constitution, Longevity lineage, and Destiny were important. Concerning 'Filial Piety and Posterity' : Showing respect to a long-lived elder and Reducing anxiety were important. Concerning 'Attachment to Life' ; The desire to live long was important. The following conclusion was made from the above results. Human beings cannot avoid death, but the span of life can be prolonged to the maximum span of human life by wisdom, health care, and proper environmental conditions. As a result, a health regimen must be imposed for longevity such as controling food intake, having positive thoughts, being relaxed, sleeping enough, clean dwelling, taking care of oneself which can improve immunity and resistance to decrease, as well as proper labor and exercise. In addition, when filial piety is served, the desire of longevity and retaining one's youthfulness for a long time can be achieved. These should be done to live long and lead a qualitative life. By the results of this research, the following is suggesed for nursing. To satisfy the basic nursing principle that nursing must be practiced to help people live long, education must be spread to people who are nursed and their family so that they can lead a healthy life. And in nursing elders, nurses must recognize that elders have a strong desire for life, even though they may have negative thoughts about life at times. Therefore nurses have to respect the elders' value of life, and help them improve their self-esteem and self -fulfilment.

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A Study on the Development of Ability Women Specialist -Focused on the nursing specialist- (여성전문인의 능력발전에 관한 연구 - 전문간호사를 중심으로 -)

  • Kwon Ill Zoo
    • Journal of Korean Public Health Nursing
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    • v.3 no.1
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    • pp.101-119
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    • 1989
  • In the present, since a five-year plan for economic development which was started in the early 60's has been successfully promoted for a quarter century, with consolidation in a department of social welfare in our country the participation. in economic society for women is more required than any other times. As a professional occupation for women is incereaing through a high-standard specialization, I think the upbringing for productive woman expert who has a strong motive of accomplishment as a developed person as well as a technical and skilful capacity which can be contributed to the growth of organization is very important. So in this study, I am evaluating the technical disposition of character of professional nurses working with hospital and also trying to supply the basic data being served to th extension of a skillful ability as a nurse, understanding the important factor related to it. The research method applied here is that we used 527 of formed questionnaires which were distributed to 7 University and General Hospitals, somewhat large in a scale, located in Seoul as an analytical material. It was performed between October 11, 1988 and October 18, 1988. An implement which was invented by Cho Moo-Sung is used after being amended and supplemented, which can measure the disposition of professional character. The formation of questionnaires of the disposition of character is 26 totally, 10 for Open-disposition, 11 for Active-disposition, 5 for wise-disposition, and it was measured. 'Ye', or 'No' through an one-half-standard and the environment of hospital organization is composed 12 questions from one point of 'Very Good' to 5 points of 'Very Bad.' Collected materials were analysed through an electronic calculation into the average value, the standard deflection, percentage, person correlative number, $X^2-test, m$ stepwise multiple regression. Summarizing the result from this research is as follows; 1. The average age of the subjective person of this investigation is 28.6 and the average career as a nurse is 6.0 years. 2. The Open-disposition that technical nurses showed is mostly half and half. 3. The Active-disposition of professional nurses was discovered affirmative largely and what they said in their questionnaires describes that they are very active answering $88.2\%$ for the disposition of self-control, $87.3\%$ for the people who think the training more seriously. 4. It was found out that the wise·disposition of technical nurse showed $90.7\%$ of 'Yes' about a new alternative of inquisitive question and we can see a progressive and profound aspect here. 5. As technical character of nurses, mutual relations between Active-disposition, Active-disposition, and wise­disposition were very profitably revealed as 0.42 in justice relations and also suggested that relations between Open-disposition, Active-disposition, and wise-disposition are 0.27 and 0.20 respectively. 6. What nurses recognize about the environment of hospital organization is reasonably acceptable while they feel very bad about rewards and punishments showing average 3.1 comparing to average 2.2 about time­control each other. Considering the prosperity of Active-disposition upon the result what I mentioned above, th possibility which is contributed to the productive improvement of hospital organization is very great and I think it can be more developed as a professional woman who has a strong motive of accomplishment, in advance.

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Contribution of Gerard Mercator's Map of 1569 for the History of Navigation (메르카토르 해도의 항해사적 공헌)

  • Kim, Sung-June;Luc, Cuyvers
    • Journal of Navigation and Port Research
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    • v.38 no.2
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    • pp.185-191
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    • 2014
  • With the 500th anniversary commemoration of Gerard Mercator's birth in 2012 now passed, there is the possibility that his name will fade back into obscurity. This would be both unfair and pitiful, because Gerard Mercator's name should be highly regarded as one of the principal contributors to navigational science and the promotion of marine safety. An accomplished cartographer, in 1569 Mercator published a remarkable 18-folio world map, depicting the then-known world in a new format with straight rhumb lines. While this distorted the size of land masses, particularly in higher latitudes, this new projection made navigation much easier for now all sailors had to do was to draw a straight line between two points to plot their course. Mercator clearly had this navigational benefit in mind, though his contemporaries did not immediately recognize its value. It wasn't until after Mercator's death, when Edward Wright (1599) and Henry Bond (1645) used and explained the new projection and demonstrated the use of straight rhumb lines in navigation that the Mercator projection became the standard for sea charts. Today, 450 years later of his death, electronic charts still rely on the projection Mercator invented and developed, confirming his position as a giant in the history of navigation. This paper introduces his life and work, detailing the importance of the 1569 world map and its contribution to navigational science and safety.

The Recognition of Occluded 2-D Objects Using the String Matching and Hash Retrieval Algorithm (스트링 매칭과 해시 검색을 이용한 겹쳐진 이차원 물체의 인식)

  • Kim, Kwan-Dong;Lee, Ji-Yong;Lee, Byeong-Gon;Ahn, Jae-Hyeong
    • The Transactions of the Korea Information Processing Society
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    • v.5 no.7
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    • pp.1923-1932
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    • 1998
  • This paper deals with a 2-D objects recognition algorithm. And in this paper, we present an algorithm which can reduce the computation time in model retrieval by means of hashing technique instead of using the binary~tree method. In this paper, we treat an object boundary as a string of structural units and use an attributed string matching algorithm to compute similarity measure between two strings. We select from the privileged strings a privileged string wIth mmimal eccentricity. This privileged string is treated as the reference string. And thell we wllstructed hash table using the distance between privileged string and the reference string as a key value. Once the database of all model strings is built, the recognition proceeds by segmenting the scene into a polygonal approximation. The distance between privileged string extracted from the scene and the reference string is used for model hypothesis rerieval from the table. As a result of the computer simulation, the proposed method can recognize objects only computing, the distance 2-3tiems, while previous method should compute the distance 8-10 times for model retrieval.

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Exploring Small Group Argumentation and Epistemological Framing of Gifted Science Students as Revealed by the Analysis of Their Responses to Anomalous Data (변칙 사례에 대한 과학 영재 학생들의 반응에서 드러난 인식론적 프레이밍과 소집단 논변활동 탐색)

  • Lee, Eun Ju;Yun, Sun Mi;Kim, Heui-Baik
    • Journal of The Korean Association For Science Education
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    • v.35 no.3
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    • pp.419-429
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    • 2015
  • In this study, we explored students' epistemological framing during scientific argumentation and how interactions among group members influenced group argumentation. Twenty-one gifted science students divided into groups of three or four participated in this study. Students' discussions related to data interpretation concerning the rate of photosynthesis were analyzed. Students' activities were videotaped in groups so the discourse could be transcribed and students' behavioral cues analyzed. Students' epistemological framing has been identified through analysis of their speech and behavioral responses to the anomalous data from the inquiry process. Subsequently, their sources of warrant and group argumentation levels were explored. We found out that group members framed the inquiry in two ways: "understanding phenomena" and "classroom game." Group members whose framing was "understanding phenomena" required other members to justify the anomalous data by examining its validity and reliability, which conclusively demonstrated a high level of argumentation. On the other hand, when group members used "classroom game" to frame their argumentation, they did not recognize the necessity of explaining the anomalous data; rather, these students used simple empirical justification to explain the data, reflecting a low level of argumentation. When students using different epistemological framing disagreed over interpretations of anomalous data throughout the discussion, clashes ensued that resulted in emotional conflict and a lack of discussion. Students' framing shifts were observed during the discussion on which group leaders seemed to have a huge influence. This study lays the foundation for future work on establishing productive framing to prompt scientific argumentation in science classrooms.

Science Education Experts' Perceptions about Necessity and Elements of Character Education in Science Education (과학교육에서 인성교육의 필요성 및 인성의 구인에 대한 전문가의 인식)

  • Kang, Eugene;Jeon, Ranyeong;Kim, Jina;Kim, Heehwa;Park, Jongseok;Son, Jeongwoo;Cho, Hyesook;Nam, Jeonghee
    • Journal of The Korean Association For Science Education
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    • v.38 no.4
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    • pp.555-563
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    • 2018
  • Recently it has been claimed that character education should be applied not only in ethics but also in other disciplines including science education. With several attempts at character education in previous research on science education, the examination of its requisite and components is needed. This research investigates the necessity and elements of character education to be improved upon in science classes through questionnaires and focus interviews of experts in the field of science education such as secondary school teachers, professors, and researchers. They recognize that character education should be adopted in science education with three backgrounds: scientist activities, the field of secondary school, and inquiry education. Based on moral education and citizenship, elements of character education in science class are comprised of four essential character elements including honesty, sympathy, tolerance, and self-esteem, and seven practical character elements that are divided into personal qualities: sincerity, responsibility, and self-control and social qualities: consideration, openness, communication, and cooperation. Their validity and importance are accepted by experts.