• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.897-899
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• 2012

In this study, spatial analyses of chemical properties were studied to find inter-relation among these properties from 5 year old general paddy field after arable land rearrangement and remodeled paddy field near 4 river project. In addition, comparison of spatial variations between two paddy fields was performed to characterize paddy fields by different formation and provide interpretation of these variations and parameters (Semivariogram and Kriging) from spatial analyses. Total of 400 ($20{\times}20$) soil samples were taken at 5 m interval from 1 ha of 5 year old general paddy field and analyzed. Total number of 54 ($6{\times}9$) soil samples were taken from remodeled paddy fields at 10m interval for the analyses. The results of pH, available Phosphate and organic matter among the analyzed results were used for interpretation. The pH values were relatively high from Gumi region. The values of available Phosphate and organic matter showed greater variant coefficients and this represented that there were greater heterogeneity in available phosphate and organic matter distributions across one paddy field. The values of skewness and kurtosis as absolute values, showed almost normal distributions. The paddy field in Ansung had available Phosphate (72.8) ${\fallingdotseq}$ pH (73.8) and greater values of organic matter (159.3), while upland in Gumi had the range value of organic (6.5) < available Phosphate (33.5) < pH (46.6). Based on these results, younger soils (0 year old) require more sampling to characterize the whole field than 5 year old soils.

• Applied Biological Chemistry
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• v.48 no.4
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• pp.339-344
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• 2005

Rice blast, which is caused by the fungus Magnaporthe grisea, is one of the most destructive diseases of rice. To identify genes involving in the signal transduction pathways that mediate rice blast resistance, we screened over 2,000 mutant lines of a highly resistant variety RIL260 that were generated by using a DEB (1, 3-Butadiene diepoxide) treatment method. In the mutant population, the frequency of albino plants was 6.7%, indicating that this population has a high frequency of mutations in the genome. The primary screening identified 29 mutant plants that exhibit a complete or partial loss of the resistance to rice blast. Among them, M5465, the most susceptible line, was subsequently examined by DNA gel-blot experiments using DNA molecular markers of Pi5(t) that has been previously identified as a durable resistance locus in RIL260. The result revealed that a large deletion and rearrangement of genomic DNA occurred in the Pi5(t) locus. The results suggest that DEB can be used as an efficient mutagen to induce large scale mutations in the rice genome. The isolated mutants should be useful for elucidating the Pi5(t)-mediated signaling pathways of rice blast resistance.

• Journal of Distribution Science
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• v.14 no.5
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• pp.107-114
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• 2016

Purpose - Loading can decrease the productivity due to the possibility of carrying out with the opposite order of storage in container terminal. When the container is to be taken out, it is needed to move the container stacked upon the container to be carried out to other place temporarily. It is called as rehandling. Remarshalling, with the loading plan, is the arranging the containers before the ship arrives in order to avoid the rehandling during the carrying out. The present study tried to find out the factors affecting the efficiency when building the remarshalling plan with the utilization of neighboring storage space as a outer slot, and analyzed the efficiency of several remarshalling algorithms with the combination of those factors. Research design, data and methodology - The present study used, when the remarshalling plan is prepared for utilizing the outer slot, the simulation methods in order to compare the efficiency of the remarshalling algorithms which made with the factors affecting the efficiency. The factors affecting the efficiency are the method of making the child node, method of arrangement, and possibility of application of FIX. In order to analyze the affecting factors on the efficiency, several algorithms are prepared with the combination of production of the child node and the arrangement method with the availability of FIX application. With this algorithm, the effect of the factors on the efficiency after building up of remarshalling plan with the target on the bay with 10 rows, 8 columns, and 10 indices. Results - The method of rearrangement and making of a child node as the factor affecting the efficiency of remarshalling utilization of the outer bay were studied. It is efficient to combine the method of making a child node with MCS in order to reduce the number of moving the containers. For reducing the time in carrying out, it was found that all arrangement methods should be combined with RCS for the efficiency. The result of experiment shows the application of FIX with good result in case of succession ratio. In addition, when FIX was not applied, all of the most combinations resulted in short time in remarshalling. As a result, it can be concluded that the algorithm with proper combination of making the child node and the arrangement can increase the job efficiency based on the importance. Conclusion - The present study suggested and analyzed the algorithms with the combination of the arrangement method, the making of child node, and FIX. It is needed to develop the algorithm to judge the possibility whether the best remarshalling plan can be built or not within the bay in order to find a better method between the two cases such as within the bay and outer the bay. As a method for extending the study on the factors affecting the efficiency, it is possible to find out the way to build the remarshalling plan within the permitted time under any storage situation.

• Journal of Korean Society of Environmental Engineers
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• v.38 no.3
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• pp.117-127
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• 2016

In this study, analyzed scenarios of the environmental instream flow for water quality improvement in Saemangeum watershed. In order to get an environmental instream flow, Methodology is selected for Retention-Basin, reservoir expansion, new dam construction, Modification of water intake and drainage system, Rearrangement of plan for system which Yongdam and Seomjin river dam have been used water supply. The study composed of diverse scenario of Environmental instream flow increasement and analyzed the effect of improving the water quality by the QUAL2K model and calculation of runoff for saemangeum watershed by SWAT model. The following water quality indicators have been simulated in irrigation and non-irrigation period for BOD and T-P. When scenarios applied to water quality model, Improvement rate in the water quality for Total Maximum Daily loads of Mankyung B unit watershed during irrigation and non-irrigation period is BOD (28.70%), T-P (17.09%) and BOD (28.51%), T-P (28.68%) respectively. Dongjin A unit watershed during irrigation and non-irrigation period is BOD (14.39%), T-P (14.59%) and BOD (15.54%), T-P (19.46%) similary. Simulation results is to quantify the constribution of the improvement in the water quality. In particular, It was demonstrative that improving effect for water quality was evaluated to be great in non-irrigation period.

• Journal of Chest Surgery
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• v.45 no.6
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• pp.380-389
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• 2012

Background: Bovine pericardium is one of the most widely used materials in bioprosthetic heart valves. Immunologic responses have been implicated as potential causes of limited durability of xenogenic valves. This study aimed to determine the effectiveness of decellularization and ${\alpha}$-galactosidase (${\alpha}$-gal) to remove major xenoreactive antigens from xenogenic tissues. Materials and Methods: Recombinant Bacteroides thetaiotaomicron (B. thetaiotaomicron) ${\alpha}$-gal or decellularization, or both were used to remove ${\alpha}$-gal from bovine pericardium. It was confirmed by ${\alpha}$-gal-bovine serum albumin-based enzyme-linked immunosorbent assay (ELISA), high-performance anion exchange chromatography, flow cytometry, 3,3'-diaminobenzidine-staining, and lectin-based ELISA. The mechanical properties of bovine pericardium after decellularization or ${\alpha}$-gal treatment were investigated by tests of tensile-strength, permeability, and compliance. Collagen fiber rearrangement was also evaluated by a 20,000${\times}$ transmission electron microscope (TEM). Results: Recombinant B. thetaiotaomicron ${\alpha}$-gal could effectively remove ${\alpha}$-gal from bovine pericardium B. thetaiotaomicron (0.1 U/mL, pH 7.2) while recombinant human ${\alpha}$-gal removed it recombinant human ${\alpha}$-gal (10 U/mL, pH 5.0). There was no difference in the mechanical properties of fresh and recombinant ${\alpha}$-gal-treated bovine pericardium. Furthermore, the TEM findings demonstrated that recombinant ${\alpha}$-gal made no difference in the arrangement of collagen fiber bundles with decellularization. Conclusion: Recombinant B. thetaiotaomicron ${\alpha}$-gal effectively removed ${\alpha}$-gal from bovine pericardium with a small amount under physiological conditions compared to human recombinant ${\alpha}$-gal, which may alleviate the harmful xenoreactive immunologic responses of ${\alpha}$-gal. Recombinant ${\alpha}$-gal treatment had no adverse effects on the mechanical properties of bovine pericardium.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.26 no.4
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• pp.355-362
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• 2000

Neoplastic growth is characterized by alterations of oncogenes and antioncogenes. The interaction between activated oncogenes and functional deletion of antioncogene appears to be the driving force directing normal cells to uncontrolled growth resulting in tumor. In addition to those genes mentioned, other genes controlling the entry of cells into the cell cycle have recently been implicated in cancer development. The overexpression of the cyclin D1 gene, which has been mapped to 11q13, either by gene rearrangement or amplification has been noted in various malignant tumors. The product of the cyclin D1 gene forms a complex with cyclin-dependent protein kinases(CDK4) that governs a key transition in the cell cycle. The relationships between the overexpression of cyclin D1 assessed by immunihistochemistry and the amplification of the cyclin D1 gene by differential polymerase chain reaction(DPCR) using primers for dopamin D2 receptor gene in 13 cases of squamous cell carcinomas of the oral cavity have been studied. The semiquantitative assay of cyclin D1 amplification has been made by cyclin D1/dopamin D2 receptor(CD/DR) ratio. The results were as follows; 1. In the normal tissue and the tumor, the CD/DR ratios were 0.82 and 1.36 respectively. This implicates 1.65-fold amplification of cyclin D1 gene in tumor compared to that in normal tissue. 2. The tumor tissue which showed overexpression of cyclin D1 by immunohistochemistry revealed 2-fold amplification of cyclin D1 compared to the normal tissue. 3. The tumor tissue which showed mild expression of cyclin D1 by immunihistochemistry revealed 1.7-fold amplification of cyclin D compared to the normal tissue. 4. The cyclin D1 was overexpressed in the tumor tissue at the rate of 38%. Above results suggest that cyclin D1 has close correlation with the development of carcinoma in the oral cavity. But further studies were needed to elucidate the carcinogeneic mechanisms by comparative studies among cyclin D1, pRb and p53.

• Tuberculosis and Respiratory Diseases
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• v.47 no.2
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• pp.272-278
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• 1999

The primary lymphoma in the lung is very rare. Moot of the primary pulmonary lymphomas, which represent 3-4 % of extra-nodal lymphomas, are low-grade B-cell lymphoma. The low-grade B-cell lymphomas progress slowly and the prognosis of these are more favorable than that of the nodal lymphomas. However, high-grade forms progress rapidly with more severe course. The diagnosis of primary pulmonary lymphomas generally relies on the histopathologic findings of lung specimens obtained by surgical excision of the lesions or open-lung biopsy. Recently, less aggressive biopsies(transbronchial, transthoracic) and/or immunocyto-chemical, immunochemical and gene rearrangement studies on materials obtained by bronchoalveolar lavage have been used occasionally. The treatment of the primary pulmonary lymphomas has not been precisely codified. Several clinical data suggest that limited surgery or non-aggressive chemotherapy can provide long-term survival in patients with such slowly developing neoplasm, and demonstrated the need for the development of noninvasive diagnootic methods. In this study, we report a case of high-grade B-cell lymphoma of the lung which was treated with combination chemotherapy.

• Genomics & Informatics
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• v.15 no.3
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• pp.87-97
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• 2017

Multiple myeloma (MM) is a malignant disease caused by an abnormal proliferation of plasma cells, of which the prognostic factors include chromosomal abnormality, ${\beta}$-2 microglobulin, and albumin. Recently, the term chromothripsis has emerged, which is the massive but highly localized chromosomal rearrangement in response to a one-step catastrophic event. Many studies have shown an association of chromothripsis with the prognosis in several cancers; however, few studies have investigated it in MM. Here, we studied the association between chromothripsis-like patterns and treatment resistance or prognosis. First, we analyzed nine MM cell lines (U266, MM.1S, RPMI8226, KMS-11, KMS-12-BM, KMS-12-PE, KMS-28-BM, KMS-28-PE, and NCI-H929) and bone marrow samples of four patients who were diagnosed with MM by next-generation sequencing-based copy number variation analysis. The frequency of the chromothripsis-like pattern was observed in seven cell lines. We analyzed the treatment-induced chromothripsis-like patterns in KMS-12-BM and KMS-12-PE cells. As a result, breakpoints and chromothripsis-like patterns were increased after drug treatment in the relatively resistant KMS-12-BM. We further analyzed the patients' results according to the therapeutic response, which was divided into sensitive and resistant, as suggested by the International Myeloma Working Group. The chromothripsis-like pattern was more frequently observed in the resistant group. In the sensitive group, the frequency of the chromothripsis-like pattern decreased after treatment, whereas the resistant group showed increased chromothripsis-like patterns after the treatment. These results suggest that the chromothripsis-like pattern is associated with treatment response in MM.

• Journal of the Korean Geographical Society
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• v.39 no.3
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• pp.321-343
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• 2004

In principal, the original form of Eupchi landscape in Naepo reflects a traditional idea regarding direction as a connection with one's fortune and naturalization strategy of power. In reality, the weight for the landscape inside the castle town was distinct by locality according to the conditions of natural geographies and main function of the castle town. In other words, the traditional Eupchi landscape was shaped under the fixed principles but it was simultaneously reflecting the local temporality and spatiality. As Chosun Dynasty went under the Japanese colonization, Eupchi in the traditional period started to evolve into a modem city. That is to say, the traditional Eupchi as a political place became to change into the center of capital accumulation, stronghold of economy and education, and center of town beyond the function as a place for government and administration. Therefore, the process of change from the landscape of Eupchi to a modem city was a kind of revolution in the form and function, and it was also a very rapid rearrangement of social space. The disparate element of landscape and double social space worked as a vital inertial element in the urban structure of Naepo area until the post independence and evolution of landscape.

• Journal of the Korean Geographical Society
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• v.50 no.1
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• pp.23-36
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• 2015

The temperature change patterns of climatic solar terms and their climatic fitness were analyzed. Harmonic analysis based on thirty-year(1981-2010) time-series data from sixty one weather stations across South Korea showed that the central peaks of the extreme heat had shifted toward start of autumn with increasing mean temperature. The overall climatic fitness of solar terms, such as major heat, frost descent, major snow, and major cold, was low, and it showed significant regional variations. The actual meteorological phenomenon representing each climatic solar term was observed much later than the day of the solar term at most weather stations. The number of observations, where an actual meteorological condition for each climatic solar term was recorded within ${\pm}1$ week from the day of that solar term, ranged only from 7.7% to 40.4% of the entire data. Study results also showed that the climatic fitness of major heat, frost descent, and major snow gradually changed in the east-west direction. Major cold, a solar term with higher climatic fitness, was influenced more strongly by latitude than longitude. Considering geographically uneven magnitude and trends in temperature changes, rearrangement and adjustment of time intervals between the solar terms may help us improve their applicability as realistic indicators of seasonal changes.