• Title/Summary/Keyword: Rearrangement

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Inhibition of ERK1/2 Activation and Cytoskeleton Rearrangement by the Recombinant Protein of Plasminogen Kringle 5 (Plasminogen kringle 5 재조합 단백질에 의한 ERK1/2 활성화 및 세포골격 재배열 억제)

  • Ha, Jung-Min;Kim, Hyun-Kyung;Kim, Myoung-Rae;Joe, Young-Ae
    • Journal of Life Science
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    • v.16 no.7 s.80
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    • pp.1199-1206
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    • 2006

  • Plasminogen kringle 5 is a potent inhibitor of endothelial tell proliferation like an endogenous angiogenesis inhibitor, angiostatin consisting of plasminogen kringles 1-4. In this study, we produced the recombinant protein of plasminogen kringle 5 (PK5) employing an Pichia expression system and examined its. effect on~endothelial cell migration and its possible inhibitory mechanism. PK5 was expressed in Pichia pastoris GS115 by fusion of the cDNA spanning from Thr456 to Phe546 to the secretion signal sequence of a-factor prepro-peptide. After methanol induction, the secreted PK5 was purified by using S-spin column. SDS-PACE analysis of the purified protein showed one major band of approximately 10kDa. In in vitro migration assays, the purified protein inhibited dose-dependently the migration of human umbilical endothelial cells (HUVECs) induced by basic fibroblast growth factor (bFGF) or vascular endothelial growth factor (VEGF) with an $IC_{50}$ of approximately 500nM. Accordingly, it inhibited bfGF-stimulated extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation in HUVECs at 500nM. In addition, it also potently inhibited bFGF-induced cytoskeletal rearrangement of HUVECs. Thus, these results suggest that Pichia-produced PK5 effectively inhibits endothelial cell migration, in part by suppression of ERK1/2 activation and blocking cytoskeleton rearrangement.

  • https://doi.org/10.5352/JLS.2006.16.7.1199 인용 PDF KSCI

RIEMANN-STIELTJES INTEGRATION OF FUNCTIONS OF $\textsc{k}{\phi}-BOUNDED$ VARIATIONS

  • JAEKEUN PARK
    • Journal of applied mathematics & informatics
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    • v.4 no.2
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    • pp.545-553
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    • 1997

  • By using the decreasing rearrangement of nonoverlapping subintervals of a closed bounded intervals and L.C.young's series for two ø-sequences we obtain some results concerning Riemann-Stieltijes integrals of functions of $\textsc{k}{\phi}-bounded$ variations.

Synthesis and Cytotoxic Activity of Flavone Derivatives

  • 안병준
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1993.04a
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    • pp.40-40
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    • 1993

  • 2-Benzoyloxyacetophenones were prepared by reaction of benzooic acids and 2-hydroxyacetophenones in the presence of dicyclohexylcarbodiimide and 4-dimethylaminopyridine. The rearrangement of 2-Benzoyloxy acetophenones to 2-Dibenzoylmethans has been carried out in the presence of tetrabutylammoniumfl uoride( a phase transfer catalyst ). Both methods have been applied first for the synthesis of flavones and gave better yields of products and the reaction ran in shorter reaction time.

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