• Korean Journal of Microbiology
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• 제44권3호
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• pp.169-177
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• 2008

Global gene expression of Deinococcus radiodurans, a highly radiation resistant bacterium, in response to excess copper was analyzed by using oligonucleotide microarray chip. Among 3,187 open reading frames of D. radiodurans, seventy genes showed a statistically significant expression ratio of at least 2-fold changes under growth conditions of excess copper; 64 genes were induced and 6 genes were reduced. Especially, two operons ($DRB0014{\sim}DRB0017$ and $DRB0125{\sim}DRB0121$) presumably involved in the iron transport and utilization were the most highly induced genes by excess copper. A quantitative real-time PCR assay revealed that DRB00l4 and DRB0125 are highly transcribed responding to excess copper and 2,2'-dipyridyl, an iron chelator. In addition, the transcription of both genes was not changed by excess iron and bathocuproine disulphonate, a copper chelator. These results suggested that the copper metabolism may be closely connected with the iron transport and utilization in D. radiodurans. However, the disruption of each gene, DRB00l4 and DRB0125, did not affect the copper and radiation resistance, the most well-known character of this organism.

• Journal of Life Science
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• 제23권4호
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• pp.510-517
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• 2013

This study was performed to evaluate the antiobesity effect of supercritical fluid extracts (SFC) and marc methanol extracts (SFM) from Cinnamomum verum in 3T3-L1 preadipocytes. In inducing the differentiation of 3T3-L1 preadipocytes in the presence of an adipogenic cocktail, iso-butylmethylanthine (IBMX), dexamathasone, and insulin, treatment with fraction residue SFC and SFM. SFC significantly reduced the mRNA expression of the transcription factor peroxisome proliferator-activate-dreceptor-${\gamma}$ ($PPAR{\gamma}$), the sterol regulatory-element-binding protein-1c (SREBP1c), and the CCAAT enhancer-binding-protein ${\alpha}$ ($C/EBP{\alpha}$) in a concentration-dependent manner. Moreover, SFC markedly down-regulated acyl-CoA synthetase-1 (ASC1), fatty acid synthesis (FAS), fatty acid transport-1 (FATP1), fatty acid binding protein 4 (FABP4), and perilipin. These findings suggest that SFC may be a potential therapeutic adjunct for obesity by targeting the differentiation of preadipocytes, as well as their functions.

• The Journal of Society for e-Business Studies
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• 제23권3호
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• pp.159-171
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• 2018

The imported foods, which are imported and sold domestically, are on the rise every year, and the scale is expected to be larger, including processing the imported raw materials. However, the origin of raw materials is indicated when declaring cargo for finished products of agricultural products, but the standardization of inspection information management system for raw materials is insufficient. In addition, there is a growing concern about the presence of residual pesticides or radioactivity in raw materials or products, and customer want to know production history information when purchasing agrifoods. It manages the hazard analysis of imported agricultural products, but most of them are global issues such as microorganisms, residual pesticides, food additives, and allergy components, etc. Therefore, it is necessary to share among the logistics entities in the entire transportation process the related data. Additionally, to do this, it needs to design an architecture and standardize business model. In this paper, it defines the architecture and the work-flow that occurs between the business process for collecting, processing, and processing information for tracking the status of imported agricultural products by steps, and develops XML message with UBL and the extracted conceptual information model. It will be easy to exchange and share information among the logistics entities through the defined standard model and it will be possible to establish visibility, reliability, safety, and freshness system for transportation of agricultural products requiring real-time management.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• 제18권5호
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• pp.91-99
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• 2019

Existing node-to-node based optimal path searching is built on the assumption that all destination nodes can be arrived at from an origin node. However, the recent appearance of the adaptive path search algorithm has meant that the optimal path solution cannot be derived in node-to-node path search. In order to reflect transportation data at the links in real-time, the necessity of the node-to-link (or link-to-node; NL) problem is being recognized. This research assumes existence of a network with link-label and non-additive path costs as a solution to the node-to-link optimal path problem. At the intersections in which the link-label has a turn penalty, the network retains its shape. Non-additive path cost requires that M-similar paths be enumerated so that the ideal path can be ascertained. In this, the research proposes direction deletion and turn restriction so that regulation of the loop in the link-label entry-link-based network transformation method will ensure that an optimal solution is derived up until the final link. Using this method on a case study shows that the proposed method derives the optimal solution through learning. The research concludes by bringing to light the necessity of verification in large-scale networks.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• 제21권3호
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• pp.30-41
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• 2022

With the advent of autonomous driving, personal mobility, drones, and smart roads, it is necessary to respond to changes in the road traffic environment in the road guidance system. However, the use of road signs to guide the road is decreasing compared to the past due to the advent of devices such as navigation and smartphones. Therefore, in this study, a large-scale survey was conducted to derive road sign issues and usage plans to respond to future changes. Based on this, this study presented a strategy to diversify road sign functions by analyzing the factors affecting the use of road signs by citizens. As a result, first, it is necessary to provide real-time variable road guidance information that reflects user needs such as traffic, weather, and local events. Second, it is necessary to informatize digital road signs such as reflecting maps with precision. Third, it is necessary to demonstrate road guidance in a virtual environment that reflects various future mobility and road environments.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• 제22권5호
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• pp.127-136
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• 2023

Gyeonggi Province has established a bus information system to provide real-time bus arrival information, aiming to make bus usage convenient for its residents. While the Gyeonggi bus information system is becoming more advanced through the application of IT technology, there are still information-vulnerable groups finding it difficult to use. In particular, the elderly have a low level of digital information literacy and habe difficulty using it. In this regard, this study aims to address the information usage disparity among the elderly in public transportation by utilizing expert in-depth survey methodology known as the Delphi technique. The study classified the policy initiatives that Gyeonggi Province should undertake into three categories: user education and expanded promotion, technological development and dissemination, and providing convenient usage environment. Through two rounds of surveys, the study assessed the priority of ten specific sub-tasks within these categories. Additionally, it gathered opinions on the effectiveness and feasibility of each item. The results yielded prioritization and evaluation of effectiveness and feasibility for nine sub-tasks. Based on these outcomes, the study proposed future projects that Gyeonggi Province should implement to address the information disparity among the elderly, offering a comprehensive approach to bridge the gap.

• Biomolecules & Therapeutics
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• 제32권1호
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• pp.154-161
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• 2024

Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disorder that causes progressive paralysis. L-Citrulline is a nonessential neutral amino acid produced by L-arginine via nitric oxide synthase (NOS). According to previous studies, the pathogenesis of ALS entails glutamate toxicity, oxidative stress, protein misfolding, and neurofilament disruption. In addition, L-citrulline prevents neuronal cell death in brain ischemia; therefore, we investigated the change in the transport of L-citrulline under various pathological conditions in a cell line model of ALS. We examined the uptake of [¹⁴C]L-citrulline in wild-type (hSOD1wt/WT) and mutant NSC-34/ SOD1^G93A (MT) cell lines. The cell viability was determined via MTT assay. A transport study was performed to determine the uptake of [¹⁴C]L-citrulline. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was performed to determine the expression levels of rat large neutral amino acid transported 1 (rLAT1) in ALS cell lines. Nitric oxide (NO) assay was performed using Griess reagent. L-Citrulline had a restorative effect on glutamate induced cell death, and increased [¹⁴C]L-citrulline uptake and mRNA levels of the large neutral amino acid transporter (LAT1) in the glutamate-treated ALS disease model (MT). NO levels increased significantly when MT cells were pretreated with glutamate for 24 h and restored by co-treatment with L-citrulline. Co-treatment of MT cells with L-arginine, an NO donor, increased NO levels. NSC-34 cells exposed to high glucose conditions showed a significant increase in [¹⁴C]L-citrulline uptake and LAT1 mRNA expression levels, which were restored to normal levels upon co-treatment with unlabeled L-citrulline. In contrast, exposure of the MT cell line to tumor necrosis factor alpha, lipopolysaccharides, and hypertonic condition decreased the uptake significantly which was restored to the normal level by co-treating with unlabeled L-citrulline. L-Citrulline can restore NO levels and cellular uptake in ALS-affected cells with glutamate cytotoxicity, pro-inflammatory cytokines, or other pathological states, suggesting that L-citrulline supplementation in ALS may play a key role in providing neuroprotection.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• 제23권2호
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• pp.119-131
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• 2024

Understanding accurate traffic performance is crucial for ensuring efficient highway operation and providing a sustainable mobility environment. On the other hand, an immediate and precise estimation of highway traffic performance faces challenges because of infrastructure and technological constraints, data processing complexities, and limitations in using integrated big data. This paper introduces a framework for estimating traffic performance by analyzing real-time data sourced from toll collection systems and dedicated short-range communications used on highways. In particular, this study addresses the data errors arising from segmented information in data, influencing the individual travel trajectories of vehicles and establishing a more reliable Origin-Destination (OD) framework. The study revealed the necessity of trip linkage for accurate estimations when consecutive segments of individual vehicle travel within the OD occur within a 20-minute window. By linking these trip ODs, the daily average highway traffic performance for South Korea was estimated to be248,624 thousand vehicle kilometers per day. This value shows an increase of approximately 458 thousand vehicle kilometers per day compared to the 248,166 thousand vehicle kilometers per day reported in the highway operations manual. This outcome highlights the potential for supplementing previously omitted traffic performance data through the methodology proposed in this study.

• Journal of Korean Society of Transportation
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• 제23권4호
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• pp.81-91
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• 2005

The Traveling Salesman Problem(TSP) is one of the NP-complete (None-deterministic Polynomial time complete) route optimization problems. Its calculation time increases very rapidly as the number of nodes does. Therefore, the near optimum solution has been searched by heuristic algorithms rather than the real optimum has. This paper reviews the Ant System Algorithm(ANS), an heuristic algorithm of TSP and its applicability in the parcel delivery service in Korea. ASA, which is an heuristic algorithm of NP-complete has been studied by M. Dorigo in the early 1990. ASA finds the optimum route by the probabilistic method based on the cumulated pheromone on the links by ants. ASA has been known as one of the efficient heuristic algorithms in terms of its calculation time and result. Its applications have been expanded to vehicle routing problems, network management and highway alignment planning. The precise criteria for vehicle routing has not been set up in the parcel delivery service of Korea. Vehicle routing has been determined by the vehicle deriver himself or herself. In this paper the applicability of ASA to the parcel delivery service has been reviewed. When the driver s vehicle routing is assumed to follow the Nearest Neighbor Algorithm (NNA) with 20 nodes (pick-up and drop-off places) in $10Km{\times}10Km$ service area, his or her decision was compared with ASA's one. Also, ASA showed better results than NNA as the number of nodes increases from 10 to 200. If ASA is applied, the transport cost savings could be expected in the parcel delivery service in Korea.

• The Journal of Korean Institute of Communications and Information Sciences
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• 제34권12A호
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• pp.925-932
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• 2009

Precise temperature monitoring is the major preconditioning to supervise quality losses within the transport chain for fresh products. ISO/IEC18000-6REV1 defines new protocols supporting BAP(Battery Assisted Passive) RFID tag which is completely compatible with EPCglobal Class1 Generation2 specification. In this paper, we designed a modem supporting BAP RFID tag with FPGA(Field Programmable Gate Array) and implemented sensor data processing function defined in ISO/IEC18000-6REV1. The transmit block of the modem supports pulse shaping filter and the output signal of the implemented RFID reader is satisfied with the spectrum mask defined in the standard. The receive block of the modem uses Gardner TED to synchronize timing of symbol. In this paper, we designed a modem supporting ISO/IEC18000-6REV1 standard and developed a RFID reader sndard. The developed RFID reader sndard can recognize sensor tag and passive tag in the wireless environment and supports real-time processing of the sensor data in the embedded linux platform.