• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.595-602
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• 2005

A one-step real-time quantitative RT-PCR assay in combination with automated RNA extraction was evaluated for routine testing of HCV RNA in the laboratory. Specific primers and probes were developed to detect 302 bp on 5'-UTR of HCV RNA. The assay was able to quantitate a dynamic linear range of $10^7-10^1$ HCV RNA copies/reaction ($R^2=0.997$). The synthetic HCV RNA standard of $1.84{\pm}0.1\;(mean{\pm}SD)$ copies developed in this study corresponded to 1 international unit (IU) of WHO International Standard for HCV RNA (96/790 I). The detection limit of the assay was 3 RNA copies/reaction (81 IU/ml) in plasma samples. The assay was comparable to the Amplicor HCV Monitor (Monitor) assay with correlation coefficient r=0.985, but was more sensitive than the Monitor assay. The assay could be completed within 3 h from RNA extraction to detection and data analysis for up to 32 samples. It allowed rapid RNA extraction, detection, and quantitation of HCV RNA in plasma samples. The method provided sufficient sensitivity and reproducibility and proved to be fast and labor-saving, so that it was suitable for high throughput HCV RNA test.

• Journal of Welding and Joining
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• v.13 no.4
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• pp.103-112
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• 1995

This study was performed to optimize friction welding conditions of Cr-Mo steel bars used for hydraulic of pneumatic valve spools and to realize the real-time evaluation of weld quality by acoustic emission method. SNCM220, SCM435, SCAM645, and SCM415 steel bars were tested to find optimum conditions of friction welding. Auantitative equations which exhibit the relations of tensile strength, elongation, reduction of area and energy absorption with friction heating time were obtained by the experiment. Acoustic emission was also performed in the friction weldig process, and the real-time evaluation was enabled to find the optimum range of weld strength. Finally, the strength and toughness of welded joints were interpreted by the sem analysis of tensile fracture surfaces.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.133.3-133
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• 2001

This paper analyses the real-time performance of Ethernet based intranet whether it is applicable to the real-time network. Unpredictability of transmission delay by collision-delay-retransmission mechanism in CAMA/CD(Carrier Sense Multiple Access with Collision Detect) of Ethernet is the major reason making hard to apply to real-time system. Both retransmission mechanism of TCP(Transmission Control Protocol) for reliability and sliding windows algorithm for high utilization make hard to predict transmission delay. Because real-time control network require fast responsibility and bustle of short-periodic messages, global-clock for collision avoidance and UDP(User Datagram Protocol) for high utilization of network are used. The mathematical models for time-delay that can be occured between ...

• Korean Journal of Food Science and Technology
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• v.45 no.1
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• pp.133-136
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• 2013

The purpose of this study was to compare a conventional culture method and real-time PCR for the detection of Yersinia enterocolitica (Y. enterocolitica) in sausage and in vegetable salad. Food samples inoculated with Y. enterocolitica were enriched in peptone-sorbitol bile-broth, and swabs were then streaked onto cefsulodin-irgasan-novobiocin agar. Biochemical tests for suspected colonies were performed with an API 20E strip. In parallel, real-time PCR was performed, targeting the 16S rRNA gene using 1 mL of enrichment broth. In sausage, the number of positive samples detected by culture method (49 out of 60) was similar (p>0.05) with that of real-time PCR (50 out of 60). However, the number of positive samples of real-time PCR (26 out of 60) was significantly higher (p<0.05) than that of the conventional culture method (6 out of 60) in vegetable salad. Real-time PCR could be an effective screening tool for detecting Y. enterocolitica, particularly in food samples with high levels of background flora, such as a vegetable salad.

• Proceedings of the KIEE Conference
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• 1993.07a
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• pp.421-423
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• 1993

A real time simulation is an effective tool for use in design, performance evaluation, and testing of the vehicle dynamic system. An alternate approach is to use a computer system designed specifically to provide an integrated simulation environment in which all aspects of hardware-in-the-loop simulation task have been taken into account.

• The Journal of the Korea institute of electronic communication sciences
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• v.17 no.1
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• pp.85-90
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• 2022

This study presents a Real-Time Power Quality(:PQ) Evaluation methodology that reflects customer's load characteristics. Author determined PQ as [Ideal], [Measured], [Acceptable], and evaluated measured PQ between Ideal and Acceptable by using Ideal AHP. Author determined Load as Resistance, Electric Motor, and Electronics, and evaluated PQ reflect Load characteristics. As future studies, author has plan to study 1:1 matrix and overall PQ evaluation including year-based PQ.

• Journal of Ocean Engineering and Technology
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• v.13 no.3 s.33
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• pp.83-91
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• 1999

Taking a view of joining by welding the IN713C to SCM440 and SCM415 steel in production of turbochargers, the frictin welding process may be utilized as a new approach for joining them of other conventional welding processes. It is because the friction welding has more technical and technical and economic advantages than the other welding processes. As this welding process has the characteristics such as curtaliment of production time and materials and cost reduction, etc.. So, this paper deals with determining the preper friction welding condition and analyzing various mechanical properties of friction welded joints of the super heat resisting steel to alloy stee(IN713C to SCM440 and SCM415). And the in-process real-time weld quality evaluation technique by acoustic emission during friction welding of IN713C to SCM440 and SCM415 steels with higher confidence and relibility has been much required even though it might be the first trial approach for developing it. Then, this first study aimed to develop the optimization of dissimilar friction welding of heat resisting steels (INC713 to SCM440, SCM415) for turbine, considering on strength and toughness.

• Journal of Power System Engineering
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• v.6 no.2
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• pp.48-53
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• 2002

Plunger (piston rod) materials are used in high-pressure condition. So STD11 is the essential material to build this plunger. However, it costs more to make a plunger by using only STD11 than using the welding of STD11 to SCM440 and other dissimilar material. And it has been difficult to weld this sort of dissimilar materials. They could be unstable in the quality by the conventional arc welding. And also they have a lot of technical problems in manufacturing. But, by the friction welding technique, it will be able to be made without such problems. And then, on account of such reasons, we need a new approach of study on real-time quality evaluation by acoustic emission (AE) techniques as well as a domestic development of the plunger by friction welding. So that, the purpose of this study for such developments is the development of a plunger by optimizing of friction welding with more reliability and more applicability. Then, this study aimed not only to develop the optimization of friction welding of dissimilar plunger steels of STD11 to SCM440, but also to develop the application technique of the acoustic emission to accomplish an in-process real-time quality evaluation during friction welding of the plunger materials by the AE technique.

• The Journal of the Korea Contents Association
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• v.17 no.10
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• pp.587-597
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• 2017

Recently, the demand for real-time performance in mobile environment is increasing due to the improvement of hardware performance, however a GPOS(General-Purpose Operating System) such as Android and Linux do not provide real-time performance. We developed RTiK(Real-Time implant Kernel) for this problem, but it has the disadvantage of supporting only x86 Architecture. In this paper, we designed and implemented a RTiKA(Real-Time implanted Kernel for ARM) to support real-time in ARM Linux. We used MCT(Multi-Core Timer) timer which replaces Local APIC Timer for real-time support, and we measured the period of generated real-time task for performance verification and evaluation. As the recent the RTiKA can guarantee the operating of several real-time tasks based on the cycle of 1ms.

• Journal of Institute of Control, Robotics and Systems
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• v.6 no.6
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• pp.433-440
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• 2000

To flexibly evaluate performance and reliability of a real-time system which is intrinsically characterized by stringent timing constraints to generate correct responses, we propose fuzzyrandom variables and build a discrete event model embedded with fuzzy-random variables. Also, we adapt fuzzy-variables to a path-space approach, which derives the upper and lower bounds of reliability by using a semi-Markov model that explicitly contains the deadline information. Consequently, we propose certain formulas of state automata properly transformed by fuzzy-random variables, and present numerical examples applying the formulas to RTP(Rapid Thermal Process) to show that a complex system can be properly evaluated based on this model by computer simulation.