• 한국신재생에너지학회:학술대회논문집
• /
• 2009.06a
• /
• pp.491-494
• /
• 2009

This paper introduces the design concepts and characteristics of WinDS3000$^{TM}$ which is a trade mark of Doosan's 3MW offshore/onshore wind turbine. WinDS3000$^{TM}$ has been designed in consideration of high RAMS (Reliability, Availability, Maintainability and Serviceability) and cost effectiveness for the TC Ia condition in GL guideline. An integrated drive train design with an innovative three-stage gearbox has been introduced to minimize nacelle weight of the wind turbine and to enhance a high reliability for transmission. A permanent magnet generator with full converter system has been introduced to get higher efficiency in part load operation, and grid friendliness use of 50 Hz and 60 Hz grid. A pitch regulated variable speed power control with individual pitch system has been introduced to regulate rotor torque while generator reaction torque can be adjusted almost instantaneously by the associated power electronics. An individual pitch control system has been introduced to reduce fatigue loads of blade and system. The wind turbine has been also equipped with condition monitoring and diagnostic systems in order to meet maintainability requirements. And internal maintenance crane in nacelle has been developed. As a result, the maintenance cost was dramatically reduced and maintenance convenience also enhanced in offshore condition.

• Horticultural Science & Technology
• /
• v.28 no.4
• /
• pp.672-677
• /
• 2010

A duplex PCR method was developed to detect a transformation vector pSPB130 used in the development of a genetically modified (GM) rose plant. To detect a GM rose plant, the anthocyanin synthase ($ANS$) was used as an endogenous reference gene of rose in PCR detection. The primer pair RHANS-KF/KR producing 107 bp amplicon was used to amplify the $ANS$ gene and no amplified product was observed in any of the 9 different plants used as a template. The primer pair GMRH-KF/KR was designed to amplify the junction sequence between 35S promoter and flavonoid 3',5'-hydroxylase ($F3^{\prime}5^{\prime}H$) gene in pSPB130. The detection limit of the duplex PCR method is approximately 0.5%. This result indicates that this duplex PCR method could be useful for monitoring unauthorized GM rose in Korea.

• Journal of Korean Society for Atmospheric Environment
• /
• v.24 no.6
• /
• pp.629-640
• /
• 2008

This study investigates long-term trends and characteristics of aerosol optical depth ($\tau_a$) and Angstrom exponent (${\AA}$) around Korea in order to understand aerosol effects on the regional climate change. The analysis period is mainly from 1999 to 2006, and the analysis sites are Anmyun and Gosan, the background monitoring sites in Korea, and two other sites of Xianghe in China and Shirahama in Japan. The annual variations of $\tau_a$ at Anmyun and Gosan have slightly systematic increasing and decreasing trends, respectively. $\tau_a$ at Anmyun shows more substantial variation, probably because of it's being closer and vulnerable to anthropogenic influence from China and/or domestic sources than Gosan. Both values at Gosan and Anmyun are approximately 1.5 times greater than those at Shirahama. The monthly variation of $\tau_a$ exhibits the highest values at late Spring and the lowest at late-Summer, which are thought to be associated with the accumulation of fine aerosol formed through the photochemical reaction before the Jangma period and the scavenging effect after the Jangma period, respectively. Meanwhile, the episode-average $\tau_a$ for the Yellow dust period increases 2 times greater than that for the non-Yellow dust period. A significant decrease in ${\AA}$ for the Yellow dust period is attributable to an increase in the loading of especially the coarse particles. Also we found no weekly periodicity of $\tau_a$'s, but distinct weekly cycle of $PM_{10}$ concentrations, such as an increase on weekdays and a decrease on weekends at Anmyun and Gosan. We expect these findings would help to initiate a study on aerosol-cloud interactions through the combination of surface aerosol and satellite remote sensing (MODIS, Calipso and CloudSat) in East Asia.

• Journal of Pharmaceutical Investigation
• /
• v.37 no.3
• /
• pp.179-186
• /
• 2007

A simple, sensitive and selective liquid chromatographic/tandem mass spectrometric method (LC-MS/MS) was developed and validated for doxifluridine and 5-fluorouracil (5-FU) quantification in dog heparinized plasma. Sample preparation was based on liquid-liquid extraction using a mixture of isopropanol/ethyl acetate (1/9 v/v) to extract doxifluridine, 5-FU and 5-chlorouracil (5-CU, an internal standard) from plasma. Chromatography was performed on a C-18 analytical column and the retention times were 2.7, 1.5 and 1.7 min for doxifluridine, 5-FU and 5-CU, respectively with shorter analysis time within 5 min than previously reported methods. The ionization was optimized using ESI negative mode and selectivity was achieved by tandem mass spectrometric analysis by multiple reaction monitoring (MRM) using the transformations of m/z 244.8>107.6, 129.0>42.0 and 144.9>42.1 for doxifluridine, 5-FU and 5-CU, respectively. The achieved low limit of quantification was 20.0 ng/mL and the assay exhibited linear range of 20-2000 ng/mL ($R^2>0.99957$ for doxifluridine and $R^2>0.99857$ for 5-FU), using $100{\mu}L$ of plasma. Accuracy and precision of quality control samples for both doxifluridine and 5-FU met KFDA and FDA Guidance criteria of 15% for accuracy with coefficients of variation less than 15%. This method demonstrated adequate sensitivity, specificity, accuracy, precision and stability to support the simultaneous analysis of doxifluridine and 5-FU in dog plasma samples in pharmacokinetic and bioequivalence studies.

• The Journal of Pediatrics of Korean Medicine
• /
• v.28 no.3
• /
• pp.1-16
• /
• 2014

Objectives The purpose of this study is to investigate the incidence and clinical features of adverse events associated with herbal medicine, and to analyze their causality and severity. Methods This study was carried out from 159 children and adolescents who took herbal medicine in the Department of Pediatrics, ${\bigcirc}{\bigcirc}$ Korean Medical Hospital from december, 2013 to april, 2014. The data was collected by survey in person or telephone. The World Health Organization (WHO)-Uppsala Monitoring Center (UMC) criteria was used to analyze causality for each adverse events. Results 1. 207 cases were surveyed from 159 children and adolescents who took one or more kinds of herbal medicine. 2. A total of 12 general adverse events (5.8%) were reported from the study. Among these adverse events, 8 cases (3.9%) were associated with herbal medicine. 3. Gastro-intestinal system disorders were most frequently reported (70%) as adverse events, which is followed by psychiatric disorders (15%), skin and appendages disorders (10%), urinary system disorders (5%). 4. The most common clinical symptom was abdominal pain (20%), followed by diarrhea (15%), loose stools (10%), vomiting (10%) and borborygmus (10%). 5. The severity of adverse drug reactions was mostly mild (87.5%), and moderate (12.5%). There was no severe case. Conclusions The adverse events from herbal medicine on children and adolescents were mostly minor, most of them could continue herbal medicines.

• Journal of Pharmaceutical Investigation
• /
• v.35 no.4
• /
• pp.287-293
• /
• 2005

The purpose of the present study was to evaluate the bioequivalence of two glimepiride tablets, Amaryl tablet (Handok & Aventis Korea, reference drug) and Mepiril tablet (Myungmoon Pharm. Co., Ltd., Korea, test drug), according to the guidelines of Korea Food and Drug Administration (KFDA). After adding an internal standard (glibenclamide) to human plasma, plasma samples were extracted using 1mL of methyl tertiary butyl ether. Compounds extracted were analyzed by reverse-phase HPLC with multiple reaction monitoring (MRM) mode analyte detection. This method for determination glimepiride proved accurate and reproducible, with a limit of quantitation of 2 ng/mL in human plasma. Twenty-four healthy male Korean volunteers received each medicine at the glimepiride dose of 2 mg in a $2{\times}2$ crossover study. There was a one-week washout period between the doses. Plasma concentrations of glimepiride were monitored by a LC-MS/MS for over a period of 12 hr after the administration. $AUC_t$ (the area under the plasma concentration-time curve from time zero to 12 hr) was calculated by the linear trapezoidal rule method. $C_{max}$ (maximum plasma drug concentration) and $T_{max}$ (time to reach $C_{max}$) were compiled from the plasma concentration-time data. Analysis of variance was carried out using logarithmically transformed $AUC_t$ and $C_{max}$. No significant sequence effect was found for all of the bioavailability parameters indicating that the crossover design was properly performed. The 90% confidence intervals of the $AUC_t$ ratio and the $C_{max}$ ratio for Amaryl/Mepiril were log 0.9583-log 1.1357 and log 1.0570-log 1.2376, respectively. These values were within the acceptable bioequivalence intervals of log 0.80-log 1.25. Taken together, our study demonstrated the bioequivalence of Amaryl and Mepiril with respect to the rate and extent of absorption.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.32 no.4
• /
• pp.95-100
• /
• 2004

This paper represents the development of a CLS(Control Loading System) for a target a airplane (KT-1) with mechanical linkage reversible flight control system. The system is composed of mechanical frame, controller, sensing part to measure the force from the stick, driving system generating the reaction forces. The DS1103 DSP(Digital Signal Processor) of the dSpace Corp. was used as the controller. The control algorithm of the CLS and the operational environment including monitoring software and evaluation tools are described. The evaluation of the system was conducted according to the requirement specification. The results of the test were analyzed by comparing with the actual data of the target airplane.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.50 no.5
• /
• pp.527-533
• /
• 2017

We investigated the infection of nervous necrosis virus (NNV) in seven band grouper Hyporthodus septemfasciatus broodstocks, which have been reared in aquaculture farms in South Korea during 2012-2014. To investigate the prevalence of NNV within the broodstock, egg, sperm, and blood were sampled in the spawning season. The egg and sperm samples were subjected to a nested reverse transcription (RT) polymerase chain reaction (PCR) assay to detect NNV and were inoculated on SSN-1 cells to culture the virus. Blood samples were used to detect antibodies against NNV using enzyme linked immunosorbent assay (ELSIA). Positive values from ELISA were found in 39 of 162 samples (24%) in 2012, and 13 of 28 samples (46%) in 2014. Additionally, 4 of 34 broodstocks (11%) investigated in 2013-2014 were determined to be carriers from the nested RT-PCR and in vitro cultivation. The broodstocks in which antibodies against NNV were detected by ELISA, or in which NNV was detected by the nested RT-PCR assay, posed a risk of vertical transmission of NNV. Therefore, it is necessary to select virus-free broodstocks in seed production to reduce the possibility of the vertical transmission of NNV.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.3
• /
• pp.1339-1344
• /
• 2014

Aims: Alterations in mitochondrial DNA (mtDNA) have been implicated in carcinogenesis and tumor progression. We here evaluated the diagnostic and prognostic potential of mtDNA as a biomarker for breast cancer. Methods: Using multiplex real-time polymerase chain reaction, nuclear DNA (nDNA) and mtDNA levels in serum, buffy coat, tumor, and tumor-adjacent tissue samples from 50 breast cancer patients were determined and assessed for associations with clinicopathological features. To evaluate mtDNA as a biomarker for distinguishing between the four sample types, we created receiver operating characteristic (ROC) curves. Results: The mtDNA levels in buffy coat were significantly lower than in other sample types. Relative to tumor-adjacent tissue, reduced levels of mtDNA were identified in buffy coat and tumor tissue but not in serum. According to ROC curve analysis, mtDNA levels could be used to distinguish between buffy coat and tumor-adjacent tissue samples with good sensitivity (77%) and specificity (83%). Moreover, mtDNA levels in serum and tumor tissue were positively associated with cancer TMN stage. Conclusions: The mtDNA levels in blood samples may represent a promising, non-invasive biomarker in breast cancer patients. Additional, large-scale validation studies are required to establish the potential use of mtDNA levels in the early diagnosis and monitoring of breast cancer.

• Journal of Pharmaceutical Investigation
• /
• v.41 no.5
• /
• pp.309-315
• /
• 2011

A sensitive and specific liquid chromatographic method coupled with tandem mass spectrometry (LC-MS/MS) was developed for the analysis of ambroxol (active moiety of acebrophylline). After acetonitrile precipitation of proteins from plasma samples, ambroxol and the domperidone (internal standard, IS) were eluted on a C18 column. The isocratic mobile phase was consisted of 10 mM ammonium acetate and methanol (10 : 90, v/v), with flow rate at 0.2 mL/min. A tandem mass spectrometer, as detector, was used for quantitative analysis in positive mode by a multiple reaction monitoring mode to monitor the m/z 379.2${\rightarrow}$264.0 and the m/z 426.2${\rightarrow}$175.1 transitions for ambroxol and the IS, respectively. Twenty four healthy Korean male subjects received two capsules (100 mg ${\times}$ 2) of either the test or the reference formulation of acebrophylline HCl in a 2 ${\times}$ 2 crossover study, this was followed by a 1week washout period between either formulation. $AUC_{0-t}$ (the area under the plasma concentration-time curve) was calculated by the linear trapezoidal rule. $C_{max}$ (maximum plasma drug concentration) and $T_{max}$ (time to reach $C_{max}$) were compiled from the plasma concentration-time data. The 90% confidence intervals for the log transformed data were acceptable range of log 0.8 to log 1.25 (e.g., log 0.8964 - log 0.9910 for $AUC_{0-t}$ log 0.8690 - log 1.0750 for $C_{max}$). The major parameters, $AUC_{0-t}$ and $C_{max}$ met the criteria of Korea Food and Drug Administration for bioequivalence indicating that Acephyll$^{(R)}$ capsule (test) is bioequivalent to Surfolase$^{(R)}$ capsule (reference).