• Title/Summary/Keyword: Reaction monitoring

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Development and Evaluation of a Carbon Dioxide Diffusive Sampling Method using Barium Hydroxide (수산화바륨을 이용한 이산화탄소 확산측정법의 개발 및 평가)

  • Yim, Bongbeen
    • Journal of Korean Society for Atmospheric Environment
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    • v.29 no.1
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    • pp.56-63
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    • 2013
  • This study was aimed at developing and evaluating a diffusive sampling method using a barium hydroxide solution as an absorbent for measuring carbon dioxide ($CO_2$) in ambient air. The collected $CO_2$ concentration was calculated by the change of conductivity resulted in the reaction of $CO_3{^{2-}}$ and $Ba^{2+}$ in aqueous solution. The sampling rate for the diffusive sampler was determined 0.218 mL/min, as obtained from the slope of the linear correlation between the $CO_2$ mass collected by the diffusive sampler and the time-weighted $CO_2$ concentration with the active sampling method. The unexposed blank sampler sealed in aluminium foil-polyethylene laminated packets has remained stable during at least one-month storage period. A good correlation was observed between the diffusive sampler and active sampler with a coefficient of determination of 0.956. This diffusive sampler would be suitable for the indoor $CO_2$ concentration monitoring.

Quantitative Real-Time PCR Assay for Detection of Paenibacillus polymyxa Using Membrane-Fusion Protein-Based Primers

  • Cho, Min Seok;Park, Dong Suk;Lee, Jung Won;Chi, Hee Youn;Sohn, Soo-In;Jeon, Bong-Kyun;Ma, Jong-Beom
    • Journal of Microbiology and Biotechnology
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    • v.22 no.11
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    • pp.1575-1579
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    • 2012
  • Paenibacillus polymyxa is known to be a plant-growth-promoting rhizobacterium. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection and quantitation of P. polymyxa using a primer pair based on the sequence of a membrane-fusion protein for the amplification of a 268 bp DNA fragment. This study reports that the qPCR-based method is applicable for the rapid and sensitive detection of P. polymyxa and can be used as an alternative method for agricultural soil monitoring.

Control System of Service Robot for Hospital (병원용 서비스 로봇의 제어시스템)

  • 박태호;최경현;이석희
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2001.04a
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    • pp.540-544
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    • 2001
  • This paper addresses a hybrid control architecture for the hospital service robot, SmartHelper. In hybrid architecture, the deliberation takes place at planning layer while the reaction is dealt through the parallel execution of operations. Hence, the system presents both a hierarchical and an heterarchical decomposition, being able to show a predictable response while keeping rapid reactivity to the dynamic environment. The deliberative controller accomplishes four functions which are path generation, selection of navigation way, command and monitoring. The reactive controller uses fuzzy and potential field method for robot navigation. Through simulation under a virtual environment IGRIP, the effectiveness of the hybrid architecture is verified.

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MEASUREMENT OF SYNTHESIS RATE OF LONG-CHAIN ACYL-COENZYME A ESTER IN BOVINE LIVER BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

  • Mitsuhashi, T.;Mitsumoto, M.;Yamashita, Y.;Ozawa, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.1 no.2
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    • pp.99-106
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    • 1988
  • A high performance liquid chromatographic procedure is described for the direct determination of the picomole amount of palmitoyl-Coenzyme A and stearoyl-Coenzyme A, using a stainless steel column packed with C-18 derivatized porous silica ($5{\mu}m$), an isocratic elution with a mixture of 33 mM $KH_2PO_4$/acetonitrile as a mobile phase and a UV detector. The long-chain acyl-Coenzyme A esters were determined in incubated microsomal fractions of a bovine liver to demonstrate the utility of this method for monitoring acyl-CoA synthesis in biological samples. The reaction rate of palmitate was higher than that of stearate. After a 60 minute incubation period, the generated amount of palmitoyl-Coenzyme A and stearoyl-Coenzyme A were approximately 70 and 20 n mol/mg micresomal protein, respectively. The advantage of this method are in that no decomposition of the CoA esters is involved, while the constituent molecular species is detected.

A Study on a Passive Sampler for Indoor Formaldehyde Using Methyl Yellow and Methyl Orange Indicator (Methyl yellow와 Methyl orange 지시약을 이용한 실내공기중의 포름알데히드 간이측정기에 대한 연구)

  • Jeong Sang Jin;Jang Jae Chul
    • Journal of Korean Society for Atmospheric Environment
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    • v.20 no.6
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    • pp.793-801
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    • 2004
  • A passive sampler has been developed for measuring formaldehyde in air using methyl yellow and methyl orange indicator. It is based on the color change of the TLC plate on reaction with formaldehyde. TLC plates impregnated with three kinds of solutions containing methyl orange, methyl yellow, mixing of methyl yellow and methyl orange were shown rectilinear response to formaldehyde concentration at constant exposure time. The color change of impregnated TLC plate with criteria air pollution, such as NO$_2$, SO$_2$, CO, $O_3$ was investigated. The color change of TLC plate with $O_3$ is larger than other gases.

Indirect Assessment on Helth affect of Air Pollutants Generated by Photo-Chemical Reaction (광화학반응에 의해 생성된 대기오염물질이 인체에 미치는 영향의 간접평가 (우리 나라 대기오존농도 추이와 문헌고찰을 중심으로))

  • 신찬기;김대선
    • Journal of environmental and Sanitary engineering
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    • v.9 no.2
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    • pp.32-40
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    • 1994
  • The concentration of ozone in 5 major cities in Korea( Seoul, Pusan, Taegu, Kwangjoo, Incheon ) has been shown increasing trend after 1984, while decreasing trend in Hsan. According to the data from 12 monitoring stations in 9 cities of metropolitan area from January 1994 to August 1994, ozone concentration exceeded short term standard 99 times and 87%(861imes) of those was occurred during July and Augusta while the maximum ozone concentration was appeared mainly between 14: 00 and 17: 00 daily. As the result of epidemiological survey, main substances which irritate eyes were identified to be PAN and formaldehyde rather than ozone while ozone was identified to be reachable to deep part of respiratory system main target organ of ozone.

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Waterborne Pathogens Identification in Public Bathroom by PCR-Reverse Blot Hybridization Assay

  • Choi, Seung-Gu;Song, Woon-Heung;Lee, Jae-Sang;Yang, Byoung-Seon;Choi, Myeong-Sik
    • Korean Journal of Clinical Laboratory Science
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    • v.43 no.3
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    • pp.120-123
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    • 2011
  • A total of 30 water samples were collected from 30 different public baths in Seoul, Korea. Contamination of public bath water by waterborne pathogens can cause disease outbreaks and contribute to increase background rates of disease. Pathogens in water was filtered by nitrocellulose membrane with $0.45{\mu}m$ pore size. The membrane filters were analyzed by both cultivation and polymerase chain reaction (PCR) amplification of partial 16S rRNA gene. Various microorganisms including 4 Escherichia coli/Shigella spp. 1 Salmonella spp. 3 Pseudomonas aeruginosa and 2 Mycobacterium spp. were identified by reverse blot hybridization assay (REBA). PCR-REBA was able to identify many bacterial genera in one assay. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bath houses.

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Fabrication of 1D Metal Oxide Nanostructures Using Glancing Angle Deposition for High Performance Gas Sensors

  • Suh, Jun Min;Jang, Ho Won
    • Journal of Sensor Science and Technology
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    • v.26 no.4
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    • pp.228-234
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    • 2017
  • Gas sensors based on metal-oxide-semiconductors are predominantly used in numerous applications including monitoring indoor air quality and detecting harmful substances such as volatile organic compounds. Nanostructures, e.g., nanoparticles, nanotubes, nanodomes, or nanofibers, have been widely utilized to improve the gas sensing properties of metal-oxide-semiconductors by increasing the effective surface area participating in the surface reaction with target gas molecules. Recently, 1-dimensional (1D) metal oxide nanostructures fabricated using glancing angle deposition (GAD) method with e-beam evaporation have been widely employed to increase the surface-to-volume ratio significantly with large-area uniformity and reproducibility, leading to promising gas sensing properties. Herein, we provide a brief overview of 1D metal oxide nanostructures fabricated using GAD and their gas sensing properties in terms of fabrication methods, morphologies, and additives. Moreover, the gas sensing mechanisms and perspectives are presented.

Review of Wheel and Lateral Force at Rail for Train running performance (열차 주행평가를 위한 윤중, 횡압 측정 방법의 검토)

  • Choi, Chan-Yong;Eum, Ki-Young;Bae, Jae-Hoon;Kim, Sang-So;Yoo, Chung-Hyun
    • Proceedings of the KSR Conference
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    • 2009.05a
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    • pp.113-118
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    • 2009
  • It is of utmost important that track loads at rail induced by running train was usually obtained for track performance evaluation. A reaction force of track was measured wheel loads and lateral force by strain gauges on the rail, and then it was obtained to derailment coefficient and variational ratio of wheel load from its relationship. In this study, a existing monitoring system methods with many manpower working were reviewed about measurements, process of testing, and how to obtain accurately measured data.

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A study of recognition system to the situation reaction (객체 정보에 대한 데이터베이스 구성 연구)

  • Park, Sangjoon;Lee, Jongchan
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2018.10a
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    • pp.161-162
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    • 2018
  • In this paper, we consider the development the database configuration to the search and management of the object information from GPS sensor and video sensor. Also, the design that the object trace of the video sensor to recognized object would be considered.

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