• Title/Summary/Keyword: Reaction monitoring

Search Result 585, Processing Time 0.04 seconds

A study for object analysis based on context awareness scenario (상황인식 시나리오 기반 객체분석에 대한 연구)

  • Song, Jiyoung
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.15 no.5
    • /
    • pp.3153-3158
    • /
    • 2014
  • Children in schoolzone accidents occur frequently in order to actively respond to the situation on the module for automated recognition research. By the vehicle penetration such like schoolzone, child object recognition, and GPS coordination information, the monitoring scenario can be constructed, and if an event occurs corresponding to strategic scenario so that suitable reaction can be provided to increase safety level to the schoolzone. In this paper, a GPS sensor and the image sensor and the monitoring server on the network based on the integration of context-aware methods have been studied. The image sensor section and the GPS section through analysis of the situation analysis and recognition of the object based on the scenario can actively cope with the situation according to the methods proposed.

Determination of more than 500 Pesticide Residues in Hen Eggs by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and Gas Chromatography-Tandem Mass Spectrometry (GC/MS/MS)

  • Golge, Ozgur;Liman, Turan;Kabak, Bulent
    • Food Science of Animal Resources
    • /
    • v.41 no.5
    • /
    • pp.816-825
    • /
    • 2021
  • This study aims to validate a fast method of simultaneous analysis of 365 LCamenable and 142 GC-amenable pesticides in hen eggs by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS), respectively, operating in multiple reaction monitoring (MRM) acquisition modes. The sample preparation was based on quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction. Key method performance parameters investigated were specificity, linearity, limit of quantification (LOQ), accuracy, precision and measurement uncertainty. The method was validated at two spiking levels (10 and 50 ㎍/kg), and good recoveries (70%-120%) and relative standard deviations (RSDs) (≤20) were achieved for 92.9% of LC-amenable and 86.6% of GC-amenable pesticide residues. The LOQs were ≤10 ㎍/kg for 94.2% of LC-amenable and 92.3% of GC-amenable pesticides. The validated method was further applied to 100 egg samples from caged hens, and none of the pesticides was quantified.

Surveillance of African swine fever infection in wildlife and environmental samples in Gangwon-do

  • Ahn, Sangjin;Kim, Jong-Taek
    • Korean Journal of Veterinary Service
    • /
    • v.45 no.1
    • /
    • pp.13-18
    • /
    • 2022
  • African swine fever (ASF) is fatal to domestic pigs and wild boars (Sus scrofa) and affects the domestic pig industry. ASF is transmitted directly through the secretions of infected domestic pigs or wild boars, an essential source of infection in disease transmission. ASFV is also very stable in the environment. Thus, the virus is detected in the surrounding environment where ASF-infected carcasses are found. In this study, ASF infection monitoring was conducted on the swab and whole blood samples from wild animals, various hematopoietic arthropod samples that could access infected wild boar carcasses or habitats to cause maintenance and spread of disease, and soil samples of wild boar habitats. ASF viral DNA detection was confirmed negative in 317 wildlife and environmental samples through a real-time polymerase chain reaction. However, ASF occurs in the wild boars and spreads throughout the Korean peninsula. Therefore, it is necessary to trace the route of ASF virus infection by a continuous vector. Additional monitoring of various samples with potential ASF infection is needed to help the epidemiologic investigation and disease prevention.

A new cell-direct quantitative PCR based method to monitor viable genetically modified Escherichia coli

  • Yang Qin;Bo Qu;Bumkyu Lee
    • Korean Journal of Agricultural Science
    • /
    • v.49 no.4
    • /
    • pp.847-859
    • /
    • 2022
  • The development and commercialization of industrial genetically modified (GM) organisms is actively progressing worldwide, highlighting an increased need for improved safety management protocols. We sought to establish an environmental monitoring method, using real-time polymerase chain reaction (PCR) and propidium monoazide (PMA) treatment to develop a quantitative detection protocol for living GM microorganisms. We developed a duplex TaqMan quantitative PCR (qPCR) assay to simultaneously detect the selectable antibiotic gene, ampicillin (AmpR), and the single-copy Escherichia coli taxon-specific gene, D-1-deoxyxylulose 5-phosphate synthase (dxs), using a direct cell suspension culture. We identified viable engineered E. coli cells by performing qPCR on PMA-treated cells. The theoretical cell density (true copy numbers) calculated from mean quantification cycle (Cq) values of PMA-qPCR showed a bias of 7.71% from the colony-forming unit (CFU), which was within ±25% of the acceptance criteria of the European Network of GMO Laboratories (ENGL). PMA-qPCR to detect AmpR and dxs was highly sensitive and was able to detect target genes from a 10,000-fold (10-4) diluted cell suspension, with a limit of detection at 95% confidence (LOD95%) of 134 viable E. coli cells. Compared to DNA-based qPCR methods, the cell suspension direct PMA-qPCR analysis provides reliable results and is a quick and accurate method to monitor living GM E. coli cells that can potentially be released into the environment.

Depletion Kinetics of the Ground State CrO Generated from the Reaction of Unsaturated Cr(CO)x with O2 and N2O

  • Son, H.S.;Ku, J.K.
    • Bulletin of the Korean Chemical Society
    • /
    • v.23 no.2
    • /
    • pp.184-188
    • /
    • 2002
  • Unsaturated $Cr(CO)_x(1{\leq}x{\leq}5)$molecules were generated in the gas phase from photolysis of $Cr(CO)_6$vapor in He using an unfocussed weak UV laser pulse and their reactions with $O_2$ and $N_2O$ have been studied. The formation and disappearance of the ground state CrO molecules were identified by monitoring laser-induced fluorescence(LIF) intensities vs delay time between the photolysis and probe pulses. The photolysis laser power dependence as well as the delay time dependence of LIF intensities from the CrO orange system showed different behavior as those from ground state Cr atoms, suggesting that the ground state CrO molecules were generated from the reaction between $O_2/N_2O$ and photo-fragments of $Cr(CO)_6$ by one photon absorption. The depletion rate constants for the ground state CrO by $O_2$ and $N_2O$ are $5.4{\pm}0.2{\times}10^{-11}$ and $6.5{\pm}0.4{\times}10^{-12}cm^3molecule^{-1}s^{-1}$, respectively.

Mariner's Information Processing Characteristics in Ship-to-Ship Collision Situation (선박간 충돌 위험상황에서의 항해사 정보처리 특성에 관한 연구)

  • Kim, Bi-A;Oh, Jin-Seok;Lee, Se-Won;Lee, Jae-Sik
    • Journal of the Korean Society of Safety
    • /
    • v.23 no.1
    • /
    • pp.46-50
    • /
    • 2008
  • The purpose of the present study was to investigate the mariner's information characteristics in ship-ta-ship collision situation using the full mission ship-handling simulator. Risk levels of ship-to-ship collision were manipulated by whether the target ship complies with the naval regulations and by movement patterns of target ship. Dependent variables reflecting mariner's information characteristics in ship-ta-ship collision situation were measured in terms of radar detection reaction time, free recall performance of past navigation situation, and subjective ratings for the task difficulty. The results showed that, in general, the mariners appeared to be deteriorated in their radar detection reaction time and free recall performance as the risk of ship-ta-ship collision increased. Also, the mariners tended to rate required tasks more difficult in the high risk ship-ta-ship collision situation.

Radionuclides in Environmental Samples and Sample Concentration of Land in the Analysis in the Method of Direct (직접법에서 환경시료중 육상시료의 방사성 핵종 및 농도 분석)

  • Jang, Eun-Sung;Kim, Jin-Seop
    • Journal of Environmental Science International
    • /
    • v.24 no.3
    • /
    • pp.275-280
    • /
    • 2015
  • In order to measure the Radionuclides and Concentration, the directly grinded land samples (river soil, pine leaves and mugwort) among the environment samples around the nuclear power plant were filled in a 450 mL Marinelli beaker and weighed to obtain the dry mass ratio of the samples. Then the background and land samples were measured for 80,000 sec. The analysis of the collected land samples showed that most of them contained less radiation nuclide than the detection minimum limit in the 'Ministry of Education, Science and Technology Public Notice No. 2010-32.'In others, the natural radionuclides $^{40}K$ were detected. Of the products of nuclear reaction discharged by a nuclear reaction, $^{134}Cs$ and $^{137}Cs$ are more easily detected, and their discharge sources can be traced using the relative ratio. Although the radioactive concentration in the vicinity of Kori Nuclear Power Plant, which is more than 1,100km away from Fukushima, the Japanese nuclear accident site, continuous monitoring is needed as the radionuclides can still be accumulated in the soil or animals and plants.

Comparison of WHO-ART Versus MedDRA, Internationally Standardized Terminology of Adverse Drug Reaction Classification (의약품 부작용에 관한 국제 분류체계인 WHO-ART와 MedDRA의 비교분석)

  • Lim, Kyung-Hwa;Shin, Hyun-Taek;Sohn, Hyun-Soon;Jun, Hyo-Jung;Lee, Joo-Hyun;Lee, Yoo-Jung;Lee, Young-Sook;Song, In-Sook
    • Korean Journal of Clinical Pharmacy
    • /
    • v.17 no.1
    • /
    • pp.46-51
    • /
    • 2007
  • This study was aimed to provide the controlled terminology for adverse drug reactions by selecting an appropriate internationally standardized classifications (WHO-ART or MedDRA). We collected the relevant information on ADR terminology systems including WHO-ART and MedDRA by online searching and visiting pharmaceutical companies and WHO UMC (Uppsala Monitoring Centre, Uppsala, Sweden). For MedDRA, project leader directly communicated with the officer of MSSO (Maintenance and Support Services Organization). Collecting all the pertinent information, two possible terminology classifications or systems (WHO-ART and MedDRA) were compared in the views of acceptability, cost-effectiveness and international feasibility and reviewed by the consultation committee and finally WHO-ART was selected.

  • PDF

Fiber-Optic Sensor Simultaneously Detecting Localized Surface Plasmon Resonance and Surface-Enhanced Raman Scattering

  • Norov, Erdene;Jeong, Hyeon-Ho;Park, Jae-Hyoung;Lee, Seung-Ki;Jeong, Dae Hong
    • Rapid Communication in Photoscience
    • /
    • v.2 no.2
    • /
    • pp.46-51
    • /
    • 2013
  • This study reports a fiber-optic sensor detecting biomolecule by simultaneously monitoring localized surface plasmon resonance (LSPR) from gold nanoparticles (Au NPs) of ca. $50{\pm}5$ nm attached on one end of optical fiber and surface enhanced Raman scattering (SERS) of the reporter molecules adsorbed on the gold surfaces as an additional sensing tool. The sensor was fabricated by immobilizing Au NPs on one end of an optical fiber by chemical reaction. LSPR and SERS signals of the sensor were measured using various refractive indices solutions. Finally, the sensor was applied to observe real-time LSPR sensor-gram and SERS spectra of the reporter molecule of 4-aminothiphenol during the antibody-antigen reaction of interferon-gamma (IFN-${\gamma}$) as a proof-concept experiment of biological applications.

Label-Free Real-Time Monitoring of Reactions Between Internalin A and Its Antibody by an Oblique-Incidence Reflectivity-Difference Method

  • Wang, Xu;Malovichko, Galina;Mendonça, Marcelo;Conceição, Fabricio Rochedo;Aleixo, José AG;Zhu, Xiangdong
    • Journal of the Optical Society of Korea
    • /
    • v.20 no.1
    • /
    • pp.165-168
    • /
    • 2016
  • Surface protein internalin (InlA) is a major virulence factor of the food-borne pathogen L. monocytogenes. It plays an important role in bacteria crossing the host's barrier by specific interaction with the cell adhesion molecule E-cadherin. Study of this protein will help to find better ways to prevent listeriosis. In this study, a monoclonal antibody against InlA was used to detect InlA. The reaction was label-free and monitored in real time with an oblique-incidence reflectivity-difference (OI-RD) technique. The kinetic constants kon and koff and the equilibrium dissociation constant Kd for this reaction were also obtained. These parameters indicate that the antibody is capable of detecting InlA. Additionally, the results also demonstrate the feasibility of using OI-RD for proteomics research and bacteria detection.