• Food Engineering Progress
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• v.13 no.2
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• pp.85-91
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• 2009

High hydrostatic pressure and enzymatic hydrolysis (HPEH) was applied to anchovy in order to produce a natural seasoning ingredient. Total soluble solid, amino nitrogen, total nitrogen and the degree of hydrolysis of anchovy hydrolysates were investigated depending on the process parameters such as temperature, pressure, enzyme concentration and enzyme type. The optimal condition for anchovy hydrolysis was confirmed as temperature 50$^{\circ}C$, reaction time 24 hrs, pressure 50 MPa and enzyme concentration 0.6% in HPEH treatment. HPEH treatment showed more effective in overall properties of anchovy hydrolysis than those of control. All anchovy hydrolysates produced by HPEH treatment were increased more 1.5-2.6 times of total free amino acid than that of control. From these results, the HPEH treatment appears to be an effective and economic process to produce a natural seasoning ingredients.

• Journal of Veterinary Science
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• v.22 no.5
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• pp.63.1-63.14
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• 2021

Background: Recently, mesenchymal stem cells therapy has been performed in dogs, although the outcome is not always favorable. Objectives: To investigate the therapeutic efficacy of mesenchymal stem cells (MSCs) using dog leukocyte antigen (DLA) matching between the donor and recipient in vitro. Methods: Canine adipose-derived MSCs (cA-MSCs) isolated from the subcutaneous tissue of Dog 1 underwent characterization. For major DLA genotyping (DQA1, DQB1, and DRB1), peripheral blood mononuclear cells (PBMCs) from two dogs (Dogs 1 and 2) were analyzed by direct sequencing of polymerase chain reaction (PCR) products. The cA-MSCs were co-cultured at a 1:10 ratio with activated PBMCs (DLA matching or mismatching) for 3 days and analyzed for immunosuppressive (IDO, PTGS2, and PTGES), inflammatory (IL6 and IL10), and apoptotic genes (CASP8, BAX, TP53, and BCL2) by quantitative real-time reverse transcriptase-PCR. Results: cA-MSCs were expressed cell surface markers such as CD90⁺/44⁺/29⁺/45^- and differentiated into osteocytes, chondrocytes, and adipocytes in vitro. According to the Immuno Polymorphism Database, DLA genotyping comparisons of Dogs 1 and 2 revealed complete differences in genes DQA1, DQB1, and DRB1. In the co-culturing of cA-MSCs and PBMCs, DLA mismatch between the two cell types induced a significant increase in the expression of immunosuppressive (IDO/PTGS2) and apoptotic (CASP8/BAX) genes. Conclusions: The administration of cA-MSCs matching the recipient DLA type can alleviate the need to regulate excessive immunosuppressive responses associated with genes, such as IDO and PTGES. Furthermore, easy and reliable DLA genotyping technology is required because of the high degree of genetic polymorphisms of DQA1, DQB1, and DRB1 and the low readability of DLA 88.

• Clean Technology
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• v.25 no.3
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• pp.238-244
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• 2019

In this study, the ignition characteristics of petroleum-based aviation fuel (Jet A-1), bio aviation fuel (Bio-6308), and blended aviation fuel (50:50, v:v) were analyzed in accordance with change of temperature and pressure. The ignition delay time of each aviation fuel was measured by combustion research unit (CRU) and the compositions of the fuels were analyzed by GC/MS and GC/FID for qualitative and quantitative results. From the results, it was confirmed that the ignition delay times of all aviation fuels were shortened with increasing temperature and pressure. In particular, the effect of temperature was larger than the effect of pressure. Also, the ignition delay time of Jet A-1 was the longest at all measurement conditions, and it was judged that this result is because of the structurally stable characteristics of the benzyl radical generated during the oxidation reaction of the aromatic compound (about 22.48%) in Jet A-1. Also, it was confirmed that Jet A-1 had no section where the degree of shortening of ignition delay time was decreased by increasing temperature, which was because the benzyl radical inhibits the response that can affect the negative temperature coefficient (NTC). The ignition characteristics of blended aviation fuel (50:50, v:v) showed a similar tendency to those of Jet A-1, rather than to those of Bio-6308, so that the blended aviation fuel (50:50, v:v) can be applied to the existing system without any change.

• Korean Chemical Engineering Research
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• v.59 no.1
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• pp.1-5
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• 2021

The PEM(Proton Exchange Membrane)water electrolysis uses the same PEM electrolyte membrane as the PEM fuel cell and proceeds by the same reaction but the opposite direction. The PEM fuel cell has many methods of degradation analysis since many studies have been conducted on the degradation and durability of the membrane and catalyst. We examined whether PEM fuel cell durability evaluation method can be applied to PEM electrolytic durability evaluation. During the PEM electrolytic degradation process, LSV(Linear sweep voltammetry), CV(Cyclic voltammetry), Impedance, SEM(Scanning Electron Microscope) and FT-IR(Fourier Transform Infrared spectroscopy) were analyzed and compared under the same conditions as the PEM fuel cell. As the PEM fuel cell, hydrogen passing through the membrane was oxidized at the Pt/C electrode, and the hydrogen permeation current density was measured to analyze the degree of degradation of the PEM membrane. Electrode degradation could be analyzed by measuring the electrode active area (ECSA) by CV under hydrogen/nitrogen flowing conditions. While supplying hydrogen and air to the Pt/C electrode and the IrO2 electrode, the impedance of each electrode was measured to evaluate the durability of the electrode and membrane.

• Korean Journal of Materials Research
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• v.33 no.8
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• pp.330-336
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• 2023

The effects of Ni²⁺ substitution for Mg²⁺-sites on the microwave dielectric properties of (Mg_1-xNi_x)(Ti_0.95(Mg_1/3Ta_2/3)_0.05)O₃ (0.01 ≤ x ≤ 0.05) (MNTMT) ceramics were investigated. MNTMT ceramics were prepared by conventional solid-state reaction. When the MgO / TiO₂ ratio was changed from 1.00 to 1.02, MgTi₂O₅ was detected as a secondary phase along with the MgTiO₃ main phase in the MNTMT specimens sintered at 1,400 ℃ for 4h. For the MNTMT specimens with MgO / TiO₂ = 1.07 sintered at 1,400 ℃ for 4h, a single phase of MgTiO₃ with an ilmenite structure was obtained from the entire range of compositions. The relative density of all the specimens sintered at 1,400 ℃ for 4h was higher than 95 %. The quality factor (Qf) of the sintered specimens depended strongly on the degree of covalency of the specimens, and the sintered specimens with x = 0.01 showed the maximum Qf value of 489,400 GHz. The dielectric constant (K) decreased with increasing Ni²⁺ content because Ni²⁺ had a lower dielectric polarizability (1.23ų) than Mg²⁺ (1.32ų). As Ni²⁺ content increased, the temperature coefficient of resonant frequency (TCF) improved, from -55.56 to -21.85 ppm/℃, due to the increase in tolerance factor (t) and the lower dielectric constant (K).

• Journal of Korea Entertainment Industry Association
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• v.13 no.4
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• pp.93-106
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• 2019

Persona is an actor's external ego constructed by playing various roles, and his/her another self-portrait in the eyes of the audience. This study was conducted to analyze persona identity containing core persona(CP) and to gain implications for the growth strategy of the actress Son Ye-jin called "melo queen" by verifying consistency between the CP and audience responses to her starring works of the past. According to the related theories and models, the persona was firstly set as image, visuality, personality and consistency, and it was used to extract and sort descriptive texts about Son related news articles in the last 5 years of the six major Korean newspapers using the content analysis method. After that, we analyzed the number of viewers of her movies and the audience share of her dramas by genre. As a result, Son's persona components were found to have a proportion for 54.2% images (34.0% for melo and romance images, 20.2% for non-melo and romance images), 25.6% for visibility, 13.8% for consistency, and 6.4% for personality. Her CP was derived from a melo and romance image. Comparing this with the audience reaction, the melo romance genre dominated and showed consistency in the drama, but in the case of the film, the non-melo romance was dominant and did not match each other. The results were attributed to a wide gap between dramas and movies in terms of key viewers, box office factors, degree of genre hybridity and experimentality. Therefore, Son should actively use her CP in the drama and challenge the various roles in order to expand her persona spectrum in the film.

• Nuclear Engineering and Technology
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• v.54 no.12
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• pp.4660-4670
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• 2022

Hydroxyurea (HU) is a novel salt-free reductant used potentially for the separation of U/Pu in the advanced PUREX process. In this work, the radiation stability of HU were systematically investigated in solution by examining the effects of the type of rays (α, β, and γ irradiations), the absorbed dose (10-50 kGy), and the HNO₃ concentration (0-3 mol L^-1). The influence degree on HU radiolysis rates followed the order of the absorbed dose > the ray type > the HNO₃ concentration, but the latter two had moderate effects on HU radiolysis products where NH₄⁺ and NO₂^- were found to be the most abundant ones, suggesting that the differences of α, β, and γ rays should be considered in the study of irradiation effects. The radiolysis mechanism was explored using density functional theory (DFT) calculations, and it proposed the dominant radiolysis paths of HU, indicating that the radiolysis of HU was mainly a free radical reaction among ·H, e_aq^-, H₂O, intermediates, and the radiolytic free radical fragments of HU. The results reported here provide valuable insights into the mechanistic understanding of HU radiolysis under α, β, and γ irradiations and reliable data support for the application of HU in the reprocessing of spent fuel.

• Journal of the Korean Society of Food Culture
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• v.23 no.4
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• pp.507-513
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• 2008

The development of soy cutlets containing textured soy protein (TSP) as a meat analog was studied. In order to decrease the beany flavor and to increase the texture, TSP was treated with 0.3% Flavourzyme or 0.1% Protamex for 10 or 20 min, respectively. The degree of hydrolysis for TSP treated with Protamex was higher than that treated with Flavourzyme. Hydrolysis was observed to increase as the reaction time was increased for both Flavourzyme and Protamex. The water holding capacity of TSP treated with Protamex for 10 min was the highest, and that treated with Flavourzyme for 20 min was similar to that of Protamex treatment for 20 min. The oil binding capacity of TSP treated with Protamex for 20 min was the highest. The hardness of the soy cutlets using TSP treated with Flavourzyme for 10 min was higher than that treated for 20 min, while that of Protamex treated for 20 min was higher than that treated for 10 min. The cohesiveness of the soy cutlets using TSP treated with Flavourzyme or Protamex for 10 min was higher than those treated for 20 min. The chewiness of the soy cutlets treated with Flavourzyme for 10 min was higher than for those treated for 20 min, while those treated with Protamex for 20 min was higher than those treated for 10 min. The springiness of TSP treated with Flavourzyme for 20 min was higher than those treated for 10 min, and higher than those treated with Protamex for 10 or 20 min. For sensory evaluation, the beany flavor of the soy cutlets treated with Protamex for 20 min was the weakest. The flavor and chewiness of both a pork cutlet and a soy cutlet treated with Protamex for 20 min were the best. In the overall quality, soy cutlets treated with Protamex for 20 min was the most desirable. In conclusion, soy cutlets treated with 0.1% Protamex for 20 min could be a reasonable substitute of pork cutlets.

• Journal of Food Hygiene and Safety
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• v.38 no.5
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• pp.381-389
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• 2023

Vibrio vulnificus, the most fatal waterborne and foodborne pathogens of 50% fatality rate in the world, is common in seawater and occurs particularly in warmer months. In this study, we investigated the toxin genes using reverse transcription-polymerase chain reaction (RT-PCR), antibiotic resistance status using Vitek, and genetic characteristics using pulsed-field gel electrophoresis (PFGE) of different V. vulnificus strains isolated from the Jeju Island seawater, distribution fishery products, and fish tanks. We examined a total of 487 samples and isolated a total of 46 strains (including overlapping strains) of V. vulnificus, 44 strains from seawater and 1 strain each from fishery products and fish tank. We detected toxin gene vvhA in all 46 strains and rtxA, viu in 8 strains (17.4%) and 9 strains (19.6%) strains, respectively. Antibiotic resistance tests indicated 100% resistance to cefoxitin antibiotics. The PFGE analysis of the 46 strains identified a total of 6 types showed 100% homology and the degree of similarity was 81.3-98.0%; however, there were no similarity between the regions and samples. These results indicate that V. vulnificus isolated from the seawater, fishery products, and fish tanks should be continuously monitored as cases of food poisoning caused by V. vulnificus with toxin genes have been reported in Jeju Island.

• Industry Promotion Research
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• v.9 no.2
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• pp.37-44
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• 2024

This study selected the worst-case scenario for fireball and vapor cloud explosion (VCE) of a styrene monomer storage tank installed in a petrochemical production plant and performed damage prediction and accident impact analysis. The range of influence of radiant heat and overpressure due to fireball and vapor VCE during the abnormal polymerization reaction of styrene monomer, the main component of the mixed residue oil storage tank, was quantitatively analyzed by applying the e-CA accident damage prediction program. The damage impact areas of radiant heat and explosion overpressure are analyzed to have a maximum radius of 1,150m and 626m, respectively. People within 1,150m of radiant heat of 4kW/m2 may have their skin swell when exposed to it for 20 seconds. In buildings within 626m, where an explosion overpressure of 21kPa is applied, steel structures may be damaged and separated from the foundation, and people may suffer physical injuries. In the event of a fire, explosion or leak, determine the risk standards such as the degree of risk and acceptability to workers in the work place, nearby residents, or surrounding facilities due to radiant heat or overpressure, identify the hazards and risks of the materials handled, and establish an emergency response system. It is expected that it will be helpful in establishing measures to minimize damage to workplaces through improvement and investment activities.