• Journal of the Korea Institute of Building Construction
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• v.17 no.4
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• pp.305-312
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• 2017

To evaluate the reliability of fly ash quality supplied to ready-mixed concrete plant using mass cylinder and hydrometer, in this research, the correlationship between the fly ash properties provided from certification and density measurement with suspension was evaluated. As a result, the reliability of the certification, except fineness and loss on ignition, all properties had a discord. Additionally, in the case of density, fineness, and L.O.I, the relation with the density measured using hydrometer showed high correlation, especially fineness was strongly related with the density measured using hydrometer. Furthermore, according to the comparative analysis with previous research, the fly ash used in this research was similar measurement with raw powder without any refining process, it is considered that the constant error of blaine test or using raw ash sample as a fly ash. In current standard regarding fly ash, the fineness range of class 2 can be changed from $3,000-4,500cm^2/g$ to $3,500-4,500cm^2/g$ for improved quality of fly ash in fineness aspect.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.4105-4105
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• 2001

Near infrared spectroscopy (NIRS) was employed to qualify and quantify on survival, the injury rate and apoptosis of the human breast cancer cell line MCF-7 cells. MCF-7 cells were cultured in RPMI medium supplemented with 10% FCS in a 95% air and 5% CO2 atmosphere at 37$^{\circ}C$. For the viable cells preparation, cells were de-touched by 0.1% of trypsin treatment and washed with RPMI supplemented with 10% FCS medium by centrifugation at 1000 rpm for 3min. For the dead cells preparation, cells were de-touched by a cell scraper. The cells were counted by a hemacytometer, and the viability was estimated by the exclusion method with frypan blue dye. Each viable and dead cells were suspended in PBS (phosphate bufferred saline) or milk at the cell density desired. For the quantitative determination of cell death by measuring the LDH (lactate dehydrogenase) activity liberated from cells with cell membrane injuries, LDH-Cytotoxic Test Wako (Wako, Pure Pharmaceutical Co. Ltd., Japan) was used. We found that NIRS measurement of MCF-7 cells at the density range could evaluate and monitor the different characteristics of living cells and dead cells. The spectral analysis was performed in two wavelength ranges and with 1,4, 10 mm pathlength. Different spectral data pretreatment and chemometrics methods were used. We applied SIMCA classificator on spectral data of living and dead cells and obtained good accuracy when identifying each class. Bigger variation in the spectra of living cells with different concentrations was observed when compared to the same concentrations of dead cells. PLS was used to measure the number of cells in PBS. The best model for measurement of dead cells, as well as living cells, was developed when raw spectra in the 600-1098 nm region and 4 mm pathlength were used. Smoothing and second derivative spectral data pretreatment gave worst results. The analysis of PLS loading explained this result with the scatter effect found in the raw spectra and increased with the number of cells. Calibration for cell count in the 1100-2500 nm region showed to be very inaccurate.

• Journal of the Korean Applied Science and Technology
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• v.38 no.1
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• pp.217-227
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• 2021

In this study, Korean-cultivated Houttuynia cordata Thunb. (HC) was extracted anti-inflammatory effects of extracts were compared in order to confirm the possibility of natural cosmetics as a raw material. HPLC pattern analysis, cell viability assay, total phenolic contents, ABTS/DPPH radical scavenging analysis, measurement of ROS production, griess reagent assay, and luminex technique. The content of quercetin was higher in fresh Houttuynia cordata (HC-F) than in dried Houttuynia cordata (HC-D). Also, content of HC extracts shows a difference in the content of the two representative compounds (polyphenol and flavonoid) according to the stored differently. ROS production showed higher inhibitory rates at HC-F. The anti-inflammatory activity of HC-D was shown higher than that of HC-F. But, in the measurement of the reduction in inflammatory cytokines, IL-1β showed that HC-D had a relatively high reduction effect, and IL-6 and TNF-α showed a high reduction effect. And these results will be a basis for the development of cosmetology in skin care and improvement of skin problem.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.1A
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• pp.19-30
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• 2012

Recently, vision-based dynamic deflection measurement techniques have significant interests and are getting more popular owing to development of the high-quality and low-price camcorder and also image processing algorithm. However, there are still several research issues to be improved including the self-vibration of vision device, i.e. camcorder, and the image processing algorithm in device aspect, and also the application area should be extended to measure three dimensional movement of floating structures in application aspect. In this study, vision-based dynamic motion measurement technique using multiple targets is proposed to measure three dimensional dynamic motion of floating structures. And also a new scheme to select threshold value to discriminate the background from the raw image containing targets. The proposed method is applied to measure the dynamic motion of large concrete floating quay in open sea area under several wave conditions, and the results are compared with the measurement results from conventional RTK-GPS(Real Time Kinematics-Global Positioning System) and MRU(Motion Reference Unit).

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.9
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• pp.1256-1263
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• 2015

Citrus junos Sieb. ex Tanaka has been traditionally called Yuza in Korea and is used as a cuisine material or tea as well as medicinal herb. In this study, we evaluated the immune-enhancing effect of Citrus junos ethanol extract (CJE) on RAW264.7 mouse macrophage and primary immunocytes. CJE treatment showed increased macrophage activity in a dose-dependant manner. CJE also enhanced natural killer (NK) cell activity. We measured lactate dehydrogenase (LDH) level as a measurement of NK cell cytotoxicity against YAC-1 lymphoma cells. CJE treatment showed an increased LDH level in a dose-dependent manner. Finally, we evaluated the effect of CJE on mouse primary splenocyte proliferation. CJE treatment slightly increased splenocyte proliferation compared to the control. The results of this study suggest that CJE can help immune function via macrophage cytokine production, increased NK cell activity, and splenocyte proliferation.

• Journal of the Korean Applied Science and Technology
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• v.34 no.3
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• pp.515-524
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• 2017

This study was intended to test anti-inflammation and cytoprotective effect against UVB after Sorghum nervosum was extracted with 70% ethanol. The efficacy of Sorghum nervosum was assessed regarding cell viability analysis, reactive oxygen species measurement, anti-inflammation, a change in COX-2 protein, and cytoprotective effect against UVB. According to the results of experiment, the cell viability of 97% or higher was shown at all concentrations of Sorghum nervosum in RAW264.7 macrophage, HaCaT cell. And in anti-inflammatory NO inhibitory activity, a concentration-dependent inhibitory effect was shown. And COX-2 protein expression was also significantly (p<.001) inhibited at 25, $50{\mu}g/mL$. With regard to cytoprotective effect against UVB, in the quantitative analysis results of reactive oxygen species within the cell, it was verified that Sorghum nervosum extract had an effect on an decrease in the total amount of ROS. When the results of study are considered comprehensively, it is thought that there is possibility of Sorghum nervosum development as raw materials for cosmetics showing an anti-inflammation and cytoprotective function against UVB.

• Journal of the Korean Society for Railway
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• v.19 no.3
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• pp.314-323
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• 2016

Overhead line voltage of DC railway traction substations has rising or falling characteristics depending on the acceleration and regenerative braking of the subway train loads. The suppression of this irregular fluctuation of the line voltage gives rise to improved energy efficiency of both the railway substation and the trains. This paper presents parameter estimation schemes using the RC circuit model for an overhead line voltage at a 1500V DC electric railway traction substation. A linear artificial neural network with a back-propagation learning algorithm was trained using the measurement data for an overhead line voltage and four feeder currents. The least square estimation method was configured to implement batch processing of these measurement data. These estimation results have been presented and performance analysis has been achieved through raw data simulation.

• Journal of Korea Multimedia Society
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• v.8 no.10
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• pp.1322-1328
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• 2005

The circulation flows passing through the Ekman boundary layer on the rotating disk and transfer the angular momentum into the interior region of the container. Consequently, the circulation enhances the momentum transfer and the interior fluid is divided by a propagating shear front. This investigation focuses on computer vision and image processing technique for analysis of Non-Newtonian Fluids. To visualize marching velocity shear front for the transient flow, a particular shaped particles and light are used. To validate the proposed method, quantitative image are compared with the optical data acquired by a direct measurement of LDV (Laser Doppler Velocimetry).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.7
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• pp.374-379
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• 2016

On-line process monitoring schemes are designed to give early warnings of process faults. In the artificial intelligence and machine learning fields, reliable approaches have been utilized, such as kernel-based nonlinear techniques. This work presents a kernel-based empirical monitoring scheme with a small sample problem. The measurement data of normal operations are easy to collect, whereas special events or faults data are difficult to collect. In such situations, noise filtering techniques can be helpful in enhancing the process monitoring performance. This can be achieved by the preprocessing of raw process data and eliminating unwanted variations of data. In this work, the performance of several monitoring schemes was demonstrated using three-dimensional batch process data. The results showed that the monitoring performance was improved significantly in terms of the detection success rate.

• Korean Journal of Medicinal Crop Science
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• v.20 no.3
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• pp.159-164
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• 2012

In this study, we investigated the antibacterial activity, antioxidative activity and whitening effect of 75% ethanol extracts from different parts of Prunus sargentii. The total phenolic compound content of the branch extract was 277.92 mg/g as the highest level. In the measurement of DPPH radical scavenging ability, $SC_{50}$ values of the cork layer and branch extract were 26.79 ${\mu}g/m{\ell}$ and 30.13 ${\mu}g/m{\ell}$. In nitric oxide (NO) scavenging ability, $SC_{50}$ values of the branch and leaf extract were 49.19 ${\mu}g/m{\ell}$ and 55.55 ${\mu}g/m{\ell}$. All extracts exhibited higher NO scavenging ability than ascorbic acid used as positive control. On the other hand, in antibacterial activity against Staphylococcus epidermidis and Staphylococcus aureus by disc diffusion assay, the pure bark extract showed the highest activity. Moreover, tyrosinase inhibitory activity of cork layer, pure bark and branch extracts showed higher activity than arbutin used as positive control. In the cytotoxicity measurement by MTT assay, leaf extract was exhibited Raw 264.7 cell viabilities of 44.68~61.83% as cytotoxic result in tested concentration. In conclusion, the branch extract of Prunus sargentii will be a functional materials without damage compared to other parts such as pure bark or cork layer in the plant.