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Antioxidant, Antibacterial and Anti-inflammatory Effects of Stachys sieboldii Extract (초석잠 추출물의 항산화, 항균 및 항염 활성)

  • Lee, Jeong Ho
    • Korean Journal of Plant Resources
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    • v.34 no.5
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    • pp.420-432
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    • 2021
  • This study was conducted to evaluate antioxidant, antibacterial, anti-inflammatory, and digestive enzyme activity in water extract (SAW) and 60% ethanol extract (SAE) from Stachys sieboldii. As the treatment concentration of each extract S. sieboldii extract increased, antibacterial and antioxidant activity increased. The total polyphenol and total flavonoid contents of SAW were 106.25 ± 0.94 mgGAE/g, 24.4 ± 0.24 mgQE/g and SAE were 124.61 ± 1.11 mgGAE/g, 45.2 ± 3.52 mgQE/g, respectively. The 400 ㎍/mL of SAW and SAE performed more than 53% protective effects against oxidative stress in HepG2 cell lines. All extracts were not showed cytotoxicity to RAW 264.7 cell line at 100 ㎍/mL. NO production was reduced to 44.3 ± 1.4% for SAW and 45.1 ± 1.0% for SAE at a concentration of 100 ㎍/mL. The production of inflammatory cytokines each TNF-α, IL-1β, and IL-6 was inhibited in a concentration-dependent. S. sieboldii extract did not showed Caco-2 cells cytotoxicity and inhibited NO production in concentration-dependent. As the concentration of the S. sieboldii extract increased of α-amylase and protease enzymes activity, which are digestive enzyme. As a result of the experiment, it is judged that it can be used as basic data for the development of health food using S. sieboldii.

Effect of the Packaging Container on the Freshness of Raw Oysters Crassostrea gigas (생굴(Crassostrea gigas)의 선도 변화에 포장용기가 미치는 영향)

  • Yoon, Na Young;An, Byoung Kyu;In, Jung Jin;Han, Hyeong Gu;Lee, Woo Jin;Seo, Jeong-Hwa;Jeong, Sam Geun;Shim, Kil Bo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.1
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    • pp.73-77
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    • 2022
  • The shelf life of oysters Crassostrea gigas, in two different types of packaging containers, polyethylene (PE) and polyethylene terephthalate (PET), was determined by evaluating the pH, glycogen and soluble protein content, turbidity, and viable cell count. After 7 days of storage, the pH of the packing water in the PE container decreased to 5.88, while the pH in the PET container decreased to 6.03. In the PE container, the glycogen content of the oysters decreased by 0.85 g/100 g and the soluble protein content and turbidity of the packing seawater increased by 1,927.21 mg/100 g and 3.24 McF, respectively. In the PET container, the glycogen content of the oysters decreased by 0.96 g/100 g and the soluble protein content and turbidity of the packing seawater increased by 1,674.75 mg/100 g and 0.98 McF, respectively. The reaction rate constants (K) were as follows: glycogen content, -0.18 (PE) and -0.10 (PET); soluble protein content, 0.29 (PE) and 0.26 (PET); and turbidity, 0.41 (PE) and 0.06 (PET). These results suggested that PET can be used as a new packaging container material for raw oysters because the quality is maintained and it offers more convenient handling during distribution.

Protein Nutritional Qualities of Beef Patties Added with Crucian Carp Extraction Residue (붕어고음 잔사분말을 첨가한 쇠고기 Patty의 단백질 품질 평가)

  • 김지영;황은영;이진화;류홍수
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.3
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    • pp.488-493
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    • 2001
  • The know the possibility in development of the low-fat beef patty models using crucian carp9 extraction residues (CCER, freeze dried powder : 5%, 10%, 15%), those protein nutritional quality, texture, color and sensory properties were investigated. About 13∼23% (on dry basis) of lipid in control was reduced in cooked beef patties with the higher addition ratios of CCER. In vitro protein digestibility was not changed in raw patties before cooking but 2∼4% higher digestibility was revealed in cooked patties. Computed protein efficiency ratio (C-PER) and discriminant computed protein efficiency ratio (DC-PER) of beef patties containing CCER were almost same as control. Lightness and red color value of both (raw and cooked) beef patties were decreased with the higher CCER addition ratios but brown color value of cooked samples were similar to control. Stronger hardness was noted in all beef patties containing CCER significantly (p<0.05). Consumer's acceptability were generally decreased by addition of CCER, but 10% level could be recommendable in beef patty processing.

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A Study on the Utilization of Yun Chun Andalusite as a Raw Material of High Alumina Refractories (I) -On the Occurrence and General Characteristics- (고 알루미나질 내화물 원료로서 연천산 홍주석의 이용에 관한 연구 (I) -홍주석의 산출상태 및 일반적특성-)

  • Ahn, Young-Pil;Choi, Long
    • Journal of the Korean Ceramic Society
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    • v.11 no.1
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    • pp.19-22
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    • 1974
  • The andalusite that can be found in mica shist near YunChun deposits were studied by means of an optical microscope, X-ray diffraction, thermal analysis of DTA and TGA, chemical analysis and refractoriness test to find out an appropriate method of concentration and purification for the utilization as a raw material of high alumina refractories, with the results: 1. The andalusite are has chiefly contained andalusite, muscovite and biotitei in addition to small amounts of chlorite, kyanite, disapore, quartz, alumandite adn pyrite, which vary from sample to sample. 2. Most of andalusite have occurred as light-red colored columns which are estimated to be an average of 4 by 0.5m/m in length. 3. Samples of andalusite which were dressed by cobbing and hand picking in the deposits have contained an average of 42% andalusite.

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Effect of Sacchromyces cerevisiae-Fermented Sophorae Radix on Production of Hydrogen Peroxide and Nitric Oxide from Macrophage Treated with Nictoine (Nicotine으로 유발된 대식세포의 hydrogen peroxide와 Nitric Oxide 생성억제에 대한 효모균발효고삼 추출물의 영향)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.5
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    • pp.1049-1054
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    • 2009
  • The effect of Sacchromyces cerevisiae-Fermented Sophorae Radix water extract (SFS) on production of hydrogen peroxide and nitric oxide (NO) from mouse macrophage Raw 264.7 Cells treated with nicotine (1 mM) was investigated through this study. SFS (0, 25, 50, 100, 200, 400 ug/mL) was simultaneously treated with nicotine (1 mM) during culture of 4, 20, 24, 44, 48, 68, and 72 hr. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. NO production after 24 hr treatement was measured with Griess reagent assay. SFS restored the production of hydrogen peroxide and NO reduced by nicotine (1 mM) in Raw 264.7 Cells. These results suggests that SFS could be supposed to have the immunological activity concerned with macrophage's oxidative burst including hydrogen peroxide and NO.

Correlation Among Items of Mechanical Tests in Raw Silk (생사기계검사 항목간의 상관관계)

  • 김영진;정지현;조동옥;이덕노
    • Journal of Sericultural and Entomological Science
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    • no.11
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    • pp.73-80
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    • 1970
  • This study was carried out in order to investigate the correlation among test items of export raw silk. The results obtained from the study are summarized as follows. 1. The highest correlation was between evenness and low evenness in case of the filaments made of autumn cocoons and the coefficient of correlation was 0.854. 2. Among following items, viz.; evenness and cleanness, neatness and low neatness and elongation and cohesion, the correlation phisically was not acknowledged, but the coefficients of correlation were high figures. 3. Poor correlation existed between following items, viz.; size deviation and maximum deviation, evenness and low evenness, neatness and low neatness, cleanness and low neatness. 4. It is considered that the high correlation exists between tenacity and elongation, but in this study the correlation existed among some items. 5. In this study it is a new fact that the correlation between neatness and cohesion as well as low neatness and tenacity existed enough highly. 6. As a conclusion the independence of ten items tested at present was acknowledged.

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Preparation for Porous Ceramics Using Low Grade Clay (저급점토를 이용한 다공성 세라믹스 제조)

  • 한상목;신대용;강상규
    • Journal of the Korean Ceramic Society
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    • v.35 no.6
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    • pp.575-582
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    • 1998
  • Sutiability of Jungsan clay shale dolomite sludge Anyang feldspar and alumina as raw materials for light-weight porous ceramics was examined. In order to find optimum manufacturing conditions compositions heating temperatuers and heating times were varied and their effects on physical properties were measured and bloating mechanism was investigated. Jungsan clay seems suitable as raw material to make the light-weight constructional materials with 5wt% of ANyang feldspar and alumina added in calcined clay (800$^{\circ}C$) having bulk density of 0.45g/cm3 water absorption of 1.34% and compressive strength of 85kg/cm2 rapid-heated at 1200$^{\circ}C$ for 30min. It is suggested that bloating mechanism depends on the difference of tem-peratures between the inside and outside in specimen the remained gases in interstices can bloat by the li-quid phase of surface with high viscosity and gas pressure at elevated temperature.

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Effects of the Extruded Pellets and Raw Fish-Based Moist Pellet on Growth and Body Composition of Flounder, Paralichthys olivaceus L. for 10 Months (상업용 부상사료와 어분기초생사료가 넙치의 성장과 체조성에 미치는 영향)

  • Cho Sung Hwoan;Lee Sang-Min;Lee Jong Ha
    • Journal of Aquaculture
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    • v.18 no.1
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    • pp.60-65
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    • 2005
  • Effects of the commercially available or formulated extruded pellets (EP) and raw fish-based moist pellet (MP) on growth and body composition of flounder Paralichthys olivaceus L. were evaluated on commercial scale for 10 months. Survivals of flounder fed the MP, EP 1 and EP3 were not significantly different from those of fish fed the EP2 or EP5, but significantly (P<0.05) higher than that of fish fed the EP4. Weight gain of fish fed the MP was not significantly different from that of fish fed the EP3 and EP5, but significantly (P<0.05) higher than that of fish fed the EP1, EP2 or EP4. Improvement in weight gain of flounder fed the high lipid diets (EP1, EP2, EP3) compared to low lipid diet (EP4) in the $55\%$ protein level, and weight gain of fish fed the high lipid diet (MP) compared to low lipid diet (EP5) in the $61\%$ protein level indicated protein-sparing effect of lipid. FER for flounder fed the EP3. which was not significantly different from that for fish fed the EP1 or EP5 was significantly (P<0.05) higher thar. that for fish fed the EP2, EP4 and MP. FER significantly (P<0.05) changed over time. CF of flounder fed the MP was significantly (P<0.05) higher than that of fish fed EP1 or EP4. Moisture content of dorsal muscle in flounder fed the EP2 was significantly (P<0.05) higher than that in fish fed the EP1 or EP4. Crude protein content of dorsal. muscle in flounder fed the EP1, EP4 and EP5 was significantly (P<0.05) higher than that in fish fed the EP2. How- ever, crude lipid content of dorsal muscle, and moisture, crude protein and crude lipid content of liver in flounder was not significantly different among the diets. In considering performance of flounder, it can be concluded that the use of EP3 and EP5 is more recommendable than the MP, which commonly used in flounder farm.

MINERAL CHARACTERISTICS OF RAW GOAT MILK FROM THE REGION OF GOIÂNIA, BRAZIL.

  • Dias, M.J.;Tanezini, C.A.;D'Alessandro, W.T.;de Oliveria, A.B.C.;Rocha, J. de M.;Pontes, I. dos S.;de Carvalho, A.L.;de Sousa, J.T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.7 no.1
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    • pp.63-68
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    • 1994
  • 432 samples of raw goat milk were analyzed for sodium, calcium, potassium, chloride, and ashes content. The samples came from 16 pure breed goats, 2 to 4 years of age kept in semi-feedlots. They were basically fed soy bran, cotton bran, triturated Guandu hay and disintegrated corn with its straw and corncob (according to requirements for due preservation and production). The mean values found were: sodium, $45{\pm}10.2mg%$ (24.5-65.6 mg%); potassium, $206{\pm}44.7mg%$ (116.6-295 mg%); calcium, $111{\pm}22mg%$ (66.5-155.4 mg %); ashes, $0.82{\pm}0.06mg%$ (0.68-0.95%); chloride, $235{\pm}39mg%$ (157-313 mg%). Results were related to: a) breed, the Brown and the Saanen revealed superior values for calcium and ashes, b) climate, which in the rain season led to higher contents of sodium, potassium, and calcium when compared to the local dry season, c) time of lactation which increasingly affected the contents of calcium, sodium and ashes, and d) the time between milking, when higher concentration of chloride and ashes were mostly concentrated in the evening and calcium in the morning. These results have important implications in the handling and management of the goat herd.

TOXICOLOGICAL STUDIES ON RAW AND PROCESSED (PREBREWED) ACONITI TUBERS; ACUTE, SUBACUTE TOXICITY STUDIES AND ASSAY OF ACONITINE ALKALOIDS (生附子와 修治附子에 관한 毒性연구 : 급성 및 아급성 독성과 Aconitine 알칼로이드 함량분석)

  • Park, Han-Soo;Kim, Seung-Hee;Kim, Pu-Young;Chang, Il-Moo
    • Toxicological Research
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    • v.6 no.1
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    • pp.41-49
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    • 1990
  • Aconiti Tuber is the root of Aconitum sp (Ranunclaceae) which has been considered as one of the most important medicinal plant having cordiotonic, diuretic and analgesic effect. On the other hand, it has been known that Aconiti Tuber contained toxic agent, aconitine alkaloids so that only processed Aconiti Tubers have been used as herbal drug traditionally. For the safety evaluation of processed Aconiti Tuber, quantitative determination of aconitine and acute, subacute toxicity test were performed on 5 commercial processed Aconiti Tubers. Arapid and precise method using HPLC has been developed for the separation and determination of aconitine. Samples were extracted with hydrochloric acid (pH3) and hot water decoction. In case of d-HCL extracts, the contents of aconitine were from 0.08 mg/g to trace. But in case of hot water decoction extracts, the contents of aconitine were not detected. For the investigation of Aconiti Tuber toxicity in rats, hot water decoction samples and methanol extracts were tested. 1) Acute toxicity test Hot water decoction sample and methanol extracts from Aconiti Tuber did not show any toxic effects in rats by an oral administration. $LD_50values of 2 extracts were above 10.0 g/kg. 2) Subacute toxicity study In the repeated administration study, hot water decoction samples were given orally to Sprague-Dawlay rats for 2 week at daily doses of 5.0 g/kg. The results are as follows; No toxic manifestation, body weight changes and lethality were observed during wxperimental period. There were no significant changes in serum enzyme activities such as GOT, GPT, LDH, ALP between treated and control groups. However CPK values were decreased in the Subuja-treated group. (P<0.01). In addition, no gross and microscopic changes were noted in Aconiti Tuber-treated groups.

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