• 생약학회지
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• 제24권3호
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• pp.255-257
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• 1993

We investigated the effects of single administration of Korean earthworm(Lamnodrilus gotai Hatai) juice (KEJ) on blood pressure and spontaneous motor activity in spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats. We report that KEJ lowered blood pressure and spontaneous motor activity in SHR.

• 약학회지
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• 제38권1호
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• pp.57-66
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• 1994

We studied the effect of eicosanoids on the content of energy metabolites and the intrasynaptosomal $Ca^{++}([Ca^{++}]_i)$ concentration in cerebral ischemic rats. An ischemic model was made by bilateral carotid artery ligation (BCAL) and by incubation of synaptosomes under aglycemic and $N_2$ gas bubbling condition. The content of ATP, creatine phosphate and glucose decreased at 15 minutes after BCAL while that of lactate increased in male Wistar rats. Oral administration of EPA(100 mg/ml/Kg/day) or DHA(16 mg/ml/Kg/day) for 6 weeks improved both the decreases and the increase of the cerebral energy metabolites. In addition, the increase of $[Ca^{++}]_i$, under BACL was suppressed by EPA or DHA treatment. When the both Wistar rats and SHR were administered orally with EPA or DHA for 6 weeks, the effect on the increase of $[Ca^{++}]_i$ under ischemia by $N_2$ gas bubbling were protected. From these results, it may be that EPA or DHA treatment were greatly contributed to preservation of ischemic cerebral energy metabolism and $Ca^{++}$ concentration.

• Natural Product Sciences
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• 제16권3호
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• pp.159-163
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• 2010

A mixture of several detoxified bacterial strains ($Sterodin^{(R)}$) has been studied for anti-inflammatory effect in Wistar rats on carrageenin, dextran and prostaglandin $E_1$ ($PGE_1$) induced edema in acute model and cotton pellet and carrageenin induced sub-acute model, while, Freund's adjuvant induced chronic model. The bacterial strains showed strong inhibitory activity in acute, sub-acute and chronic models of inflammation. Further, it reduced ${\alpha}1$ acid glycoprotein and ${\alpha}2$ macroglobulin levels in serum and prostaglandin $E_2$ in inflamed paw. These results indicated that the bacterial strains probably act through prostaglandin mediatory pathways and may be useful in treatment of inflammation.

• Journal of Chest Surgery
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• 제32권3호
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• pp.215-223
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• 1999

배경: 여러 가지 다양한 원인에 의해 기도 협착이 초래된 경우, 단단문합이 가능한 병변을 제외하고는, 기관을 대치할 수 있는 여러 가지 대용물이 고려되고 임상에서도 이용되고 있으나 아직 만족스러운 결과는 보고되고 있지 않다. 이러한 이유로 비가역적 손상을 받은 다른 장기 \ulcorner마찬가지로 기관의 이식술이 고려되고 공여 기관의 자원의 제한으로 인하여 장기간 기관의 보관이 필요하게 되었다. 이에 쥐를 이용한 기관의 이소이식술을 통하여 기관 이식의 임상 적용 가능성 및 초냉동 보관을 통한 장기간 보관의 실험 모델의 정립 및 형태학적 측면에 대한 연구를 시행하였다. 대상 및 방법: 30마리의 Wistar쥐에서 60개의 기관 절편을 취득하여 20마리의 Wistar쥐와 40마리의 Sprague Dawley쥐들의 복부 복강내에 이소 이식하였다. 이식 동물 대상에 따라 실험군은 모두 6개의 군으로 나누었다. I, II, III군은 기관 절편을 취득후 곧바로 이식한 군들이며, IV, V, IV군은 기관 절편을 1개월간 -196$^{\circ}C$에서 초냉동 보관시킨 다음 이식한 군들이다. I군과 IV군은 동인자형 대조군으로 이식 대상 쥐는 공여 쥐와 같은 Wistar쥐였으며 면역억제제를 투여하지 않았다. II군과 V군은 이인자형 이식군으로 모두 Sprague-Dawley쥐로서 면역억제제를 투여하지 않았고, III군과 VI군도 마찬가지로 이인자형 이식군이었으나 면역억제제를 투여하였다. 모든 쥐는 이식술후 28일째 절명시키고 이소 이식된 기관 절편을 분리하여 조직학적 검사를 시행하였다. 결과: 기관 내강 및 상피 세포의 변화는 초냉동 보관의 시행 유무와는 차이가 없었으며, 면역억제제의 투여 유무에 따라 현저한 차이가 관찰되었다(p<0.01). 면역억제제를 투여하였던 III군과 VI군의 경우 기관 내강이 잘 유지되어 있었고 정상 상피 세포의 관찰이 가능하였으나, 나머지 군의 대부분의 기관절편에서 기관 내강이 섬유 조직의 증식에 의해 거의 폐쇄되어 있었고, 상피세포의 소실 및 상피하 조직의 관찰이 힘들었다. 이러한 기관 내강의 섬유 조직의 증식은 폐이식술과 관련된 폐색성 세지관지염의 병리 소견과 유사하였다. 결론: 쥐 기관의 초냉동 보관후의 이소 이식술시 적절한 면역억제제의 투여로 기관 내강 및 기관 상피세포가 잘 유지됨을 알 수 있어, 보다 장기간의 기관 보관이 가능할 것으로 생각된다. 그러나 초냉동 보관의 시행 여부와는 상관없이 면역억제제를 투여하지 않은 군에서 폐색성 모세기관지염과 비슷한 양상의 기관 내강의 섬유조직 과잉 증식 및 기관 상피세포의 소실 등이 관찰되었다. 쥐와 같은 소동물에서의 이러한 시도는 폐이식술과 관련된 어려운 문제중의 하나인 폐색성 모세기관지염의 병인, 예방 및 치료를 위한 연구에 있어 좋은 실험 모델이 될 수도 있을 것이다.

• Preventive Nutrition and Food Science
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• 제15권1호
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• pp.1-6
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• 2010

The effect on body fat accumulation on male Wistar rats undergoing continuous feeding with a histidine-enriched diet was investigated. Five-week-age rats were assigned to two groups and were fed either the control diet (purified diet AIN-$76^{TM}$) or the histidine-enriched diet containing 3% histidine for 28 days. It was observed that both adipose tissue masses in retroperitoneal and epididymal areas of rats fed histidine-enriched diet significantly decreased (p<0.05) compared to those of control rats, while there was no significant difference in the food efficiency ratio between them. The blood levels of histidine derivatives of 3-methylhistidine and carnosine were significantly (p<0.05) increased in the rats fed a histidine-enriched diet, whereas there were no significant different between the histidine-enriched diet and control groups in the general amino acid distribution. Our results demonstrate that a histidine-enriched diet suppresses body fat accumulation in rats.

• 셀메드
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• 제13권6호
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• pp.5.1-5.8
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• 2023

Objectives: Cassia occidentalis L. is a weed belonging to the Caesalpiniaceae family. The root of this medicinal plant is used for the treatment of various ailments, including kidney diseases. The present study was aimed at evaluating the nephroprotective effects of HAE of the roots of Cassia occidentalis L. against gentamicininduced renal toxicity in albino Wistar rats. Methods: The renal toxicity was induced by subcutaneous administration of gentamicin at 100 mg/kg in the rats belonging to the disease control and treatment groups from the 4^th to the 8^th day. The rats in the treatment group received HAE of the roots of Cassia occidentalis L. at 67 mg/kg b. w. orally for 8 days, while no treatment was given to the rats in the disease control and plain control groups. At the end of the experiment, renal biomarkers viz; s. creatinine, b. urea, and s. uric acid, were investigated. The histopathological examination of the kidney specimens was also carried out. Results: The results of the present study revealed that renal function biomarkers such as s. creatinine, b. urea, and s. uric acid were significantly reduced in the rats of the treatment group as compared to those of the disease control group. Moreover, the histoarchitecture reports of the treatment group's kidney specimens showed significant improvements. Conclusion: The results suggested that the HAE of Cassia occidentalis L. roots promisingly prevented kidney injury in gentamicin-induced nephrotoxic rats. This effect might be due to improved clearance of gentamicin from the renal tubule and decreased generation of reactive oxygen species (ROS).

• Natural Product Sciences
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• 제26권3호
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• pp.252-258
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• 2020

This study investigated the biochemical and histological changes associated with the co-administration of cisplatin and methanolic extract of Portulaca oleracea (MEPO) in adult Wistar rats. Twenty-four (24) adult female Wistar rats were randomly divided into six (6) groups (A-F) (n = 4). Group A served as the control group for the experiment and received no treatment. Group B was given a single dose of cisplatin and served as the cisplatin control group. Group C and D received 400 mg/kg and 800 mg/kg of MEPO 6 hours after a single dose cisplatin injection respectively. Group E and F received 400 mg/kg and 800 mg/kg of MEPO 6 hours before cisplatin injection. The cisplatin injection was 2 mL/kg given intraperitoneally for all groups. There was a significant increase in the serum levels of ALT, ALP, AST, total bilirubin, and conjugated bilirubin following cisplatin treatment (p = 0.000, 0.000, 0.039, 0.000, 0.004 respectively) with a consequent reversal due to MEPO administration across all treated groups (p = 0.000, 0.000, 0.000, and 0.000) in a dose-dependent fashion. Cisplatin caused the expansion of the red and white pulp in the spleen which was attenuated by MEPO. MEPO demonstrated a protective effect against cisplatin-induced liver and spleen toxicity.

• Clinical and Experimental Reproductive Medicine
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• 제47권3호
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• pp.168-179
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• 2020

Objective: This study investigated testicular oxidative stress status and physiomorphological function in Wistar rats fed with yaji and cadmium chloride (CdCl₂). Methods: Sixty male albino Wistar rats (12 per group) were randomly assigned to five groups: group I (control), group II (300 mg/kg.bw of yaji), group III (500 mg/kg.bw of yaji), group IV (2.5 mg/kg.bw of CdCl₂), and group V (2.5 mg/kg.bw of yaji+4 mg/kg.bw omega-3). Each group was evenly subdivided into two subgroups and treatment was administered for 14 days and 42 days, respectively. Semen quality (sperm count, progressive motility, normal morphology, and gonadosomatic index), hormones (testosterone, follicle-stimulating hormone, and luteinizing hormone), testicular oxidative stress markers (superoxide dismutase, catalase, glutathione peroxidase, and malonaldehyde) and testicular histomorphological features were examined. Results: Yaji caused significant (p< 0.05) dose- and duration-dependent reductions in semen quality, the gonadosomatic index, testosterone, follicle-stimulating hormone, and luteinizing hormone. Yaji also caused significant (p< 0.05) dose- and duration-dependent decreases in superoxide dismutase, catalase, and glutathione peroxidase activity, as well as increased testicular malonaldehyde levels. Yaji induced distortions in the testicular histological architecture. CdCl₂ damaged testicular function by significantly (p< 0.05) reducing semen quality, reproductive hormone levels, and oxidative stress markers in albino Wistar rats. CdCl₂ also altered the histology of the testis. Conclusion: This study shows that yaji sauce has similar anti-fertility effects to those of CdCl₂, as it adversely interferes with male reproduction by impairing oxidative stress markers and the function and morphological features of the testis.

• 셀메드
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• 제10권1호
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• pp.2.1-2.8
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• 2020

Uterotonics have the ability to contract uterus. Such plants might be useful in augmenting or inducing labour, expelling retained afterbirth and for abortifacient purposes. Limitations associated with conventional treatments have made herbal medicines a feasible alternative for the management of these conditions. The aim of this study was to evaluate the contractile effects of Uvariodendron kirkii extracts on isolated uterine strips of female Wistar rats. Isolated strips of Wistar rats' uteri were treated with 20, 40, 80 and 160 mg/ml concentrations of Uvariodendron kirkii aqueous extract. The plant extract was also tested against prostaglandin and oxytocin induced uterine contractions. Uvariodendron kirkii extract concentrations (20, 40, 80 and 160 mg/ml) increased the frequency of uterine contraction (16.53, 25.12, 33.48 and 56.39 percentages respectively) compared to the control. The graded extract concentrations caused a significant increase in amplitude (force) of uterine contractions by 2.87, 9.22, 16.37 and 24.32 percentages respectively. The concentrations significantly increased the frequency of oxytocin induced uterine contractions by 6.92; 28.31; 47.06, 58.78 percentages respectively. The graded extract concentrations also significantly increased the amplitude of oxytocin induced uterine contractions by 6.07; 9.40; 15.19 and 23.56 percentages respectively. Uvariodendron kirkii extract concentrations significantly increased the frequency and amplitude of prostaglandin induced contractions. The percentage increase in frequency was 11.44, 8.92, 20.65 and 35.71 at 20, 40, 80 and 160 mg/ml respectively. The mean amplitude of prostaglandin induced uterine contractions also increased (4.75, 3.89, 8.29 and 15.91% at 20. 40, 80 and 160 mg/ml respectively). The extract caused a dose dependent increase in uterine frequency and amplitude of contraction. The findings of thisstudy are useful in generating a novel uterotonic agent that will be useful in augmenting labour or in expelling retained after birth in cattle. More studies at molecular level will further elucidate the plant mechanism of action.

• Food Science and Biotechnology
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• 제18권6호
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• pp.1330-1335
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• 2009

Although beneficial effects of dietary plant proteins on lipid metabolism are well documented, not much information exists on the influence of different seafood proteins on the lipid metabolism. The present study evaluated the effect of 2 marine proteins (tuna protein and scallop ovary proteins) in comparison to casein and soy protein in male Wistar rats. The concentration of total lipids in the plasma of rats fed experimental diets was significantly lower from that of control (278.2 mg/dL) group (p<0.05); and, the liver lipid content was not significantly different (p>0.05). Fecal excretion of cholesterol and bile acids was significantly higher in marine proteins and soy protein fed groups compared to casein only fed control (6.1 and 6.4 mg/day, respectively) group (p<0.05). No significant differences were observed in the mRNA concentrations of different transcriptional factors (p>0.05).