• Biotechnology and Bioprocess Engineering:BBE
• /
• v.8 no.2
• /
• pp.59-63
• /
• 2003

The protein disulfide isomerase (PDI) reaction kinetics has been studied to evaluate its effect on the monoclonal antibody (Mab) refolding and assembly which accompanies disulfide bend formation. The MAb in vitro assembly experiments showed that the assembly rate of heavy and light chains can be greatly enhanced in the presence of PDI as compared to the rate of assembly obtained by the air-oxidation. The reassembly patterns of MAb in-termediates were identical for both with and without PDI, suggesting that the PDI does not determine the MAb assembly pathway, but rather facilitates the rate of MAb assembly by promoting PDI catalyzed disulfide bond formation. The effect of growth rate on PDI activities for MAb production has also been examined by using continuous culture system. The specific MAb productivity of hybridoma cells decreased as the growth rate increased. However, PDI activities were nearly constant fur a wide range of growth rates except very high growth rate, indicating that no direct correlation between PDI activity and specific MAb productivity exists.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.1 no.1
• /
• pp.13-17
• /
• 1996

The protein disulfide isomerase(PDI) reaction kinetics has been studied to evaluate its effect on the monoclonal antibody(MAb) refolding and assembly which accompanies disulfide bond formation The MAb in vitro assembly experiments showed that the assembly rate of heavy and light chains can be greatly enhanced in the presence of PDI as compared to the rate of assembly obtained by the air-oxidation. The reassembly patterns of MAb intermediates were identical for both with and without PDI, suggesting that the PDI does not determine the MAb assembly pathway, but rather facilitates the rate of MAb assembly by promoting PDI catalyzed disulfide bond formation. The effect of growth rate on PDI activities for MAb production has also been examined by using continuous culture system. The specific MAb productivity of hybridoma cells decreased as the growth rate increased. However, PDI activities were nearly constant for a wide range of growth rates except very high growth rate, indicating that no direct correlation between PDI activity and specific MAb productivity exists.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.1 no.1
• /
• pp.22-25
• /
• 1996

An Exponetial feeding strategy has been frequently used in fed-batch fermentation of recombinant E. coli. In this feeding scheme, growth yield and initial cell concentration, which can be erroneously determined, are needed to calculate the feed rate for controlling specific growth rate at the set point. The effect of the incorrect growth yield and initial cell concentration on the control of the specific growth rate was theoretically analyzed. Insignificance of the correctness of those parameters for the control of the specific growth rate was shown theoretically and experimentally.

• Journal of the Korean Society of Combustion
• /
• v.19 no.4
• /
• pp.49-55
• /
• 2014

Design criterion for the size of micro Pt-catalytic combustor is investigated in terms of heat release rate. One-dimensional plug flow model is applied to determine the surface reaction constants using the experimental data at stoichiometric butane-air mixture. With these reaction constants, the mass fraction of butane and heat release rate predicted by the plug flow model are in good agreement with the experimental data at the combustor exit. The relationship between the size of micro catalytic combustor and mixture flowrate is introduced in the form of product of two terms-the effect of fuel conversion efficiency, and the effect of chemical reaction rate and mass transfer rate.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.11 no.10
• /
• pp.5080-5097
• /
• 2017

Channel characteristics-based secret key generation is an effective physical-layer security method. The issues of how to remove the effect of random noise and to balance the key generation rate (KGR) and the bit mismatch rate (BMR) are needed to be addressed. In this paper, to reduce the effect of random noise and extract more secret bits, a new quantization scheme with high key generation rate and low bit mismatch rate is proposed. In our proposed scheme, we try to use all measurements and correct the differences caused by noise at the boundary regions instead of simply dropping them. We evaluate and discuss the improvements of our proposed scheme. The results show that our proposed scheme achieves lower bit mismatch rate as well as remaining high key generation rate.

• Journal of the Korean Vacuum Society
• /
• v.5 no.3
• /
• pp.263-267
• /
• 1996

Reactive ion etching of Pt thinfilm was studied using $CCl_2F_2$, Ar, and $O_2$ . Etch rate of the Pt increased as the total pressure decreases and the RF power increased, while the flow rate of $CCl_2F_2$ had little effect on the Pt etch rate. Addition of $O_2$ had no effect on Pt etch rate up to 20% $O_2$ Selectivity between Pt and photoresist increased as the pressure decreased and the RF power increased, making it possible to pattern a thicker Pt layer with a thinner photoresist. A maximum etch rate of 300$\AA$/min was obtained at $CCl_2F_2$ flow rate of 20 sccm. RF power of 400 W, and the total pressure of 60mTorr.

• Journal of the Korean Society for Heat Treatment
• /
• v.16 no.6
• /
• pp.315-319
• /
• 2003

Effect of nitriding on fatigue crack initiation and growth rate has been studied in Ni-Cr-Mo steel. Specimens were nitrided at $860^{\circ}C$ for 15 hr. The fatigue limit of nitrided specimens were superior to those ofannea1ed($860^{\circ}C$, 15 hr) specimens. Based on detailed observations of slip band and micro crack initiation, it is concluded that the excellent fatigue limit of nitrided specimens is attributed to improved slip initiation resistance by nitriding. The characteristic of fatigue crack growth rate of nitrided specimens was investigated by comparing with those of annealed specimens. It was found that the crack growth rate was markedly decreased and the threshold stress intensity factor range was improved by nitriding. It is concluded that the excellent fatigue limit of nitrided specimens is also attributed to improved fatigue crack growth rate and threshold stress intensity factor range by nitriding.

• Journal of Korean Society on Water Environment
• /
• v.23 no.5
• /
• pp.577-580
• /
• 2007

The influence of flow rate has been investigated on the treatment efficiency of continuous cycling electrolytic process employing artificial and actual photographic wastewater which containing silver ion. For artificial wastewater, the treatment efficiency of process was found to rise ca. three times when the flow rate of wastewater was increased from 3 mL/min to 15 mL/min. The process efficiency was doubled under the same condition regarding actual wastewater. The effect of flow rate on the treatment efficiency was observed to be altered according to the metal ionic form and solution composition. The coefficient of mass transfer was estimated using model equation, which verified that the raised treatment efficiency at higher flow rate was due to the increased mobility of ionic species.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
• /
• 2001.04a
• /
• pp.95-100
• /
• 2001

Recently, coated milling cutters are widely used for improving the productivity of cutting processes through high speed cutting and longer tool life. In metal cutting, cutting force increase rate is important factor to diagnose the cutting conditions because the amount of tool wear directly influences the cutting forces. As the cutting length increases, the worn cutter increases the cutting forces. In this study, the effect of coating process of end milling cutter on the cutting performance, especially on the cutting force increase rate, is investigated. The results acquired through the cutting test measuring cutting force increase rate show that not only the injection quantity of $N_2$ and Ar but also mean temperature influence the cutting force increase rate during the end milling process.

• Journal of Biosystems Engineering
• /
• v.25 no.2
• /
• pp.89-96
• /
• 2000

In this study, experiments were performed for various drying air temperatures, air flow rates tray distance to analyze drying characteristics of batch type tunnel dryer. In comparison of tunnel drying with cabinet drying which is currently used in the farm, the results of drying simulation model of cabinet dryer was used and then the possibility of applying the drying simulation model of cabinet dryer to batch type tunnel dryer was investigated. The results showed that as the drying temperature increased, the drying rte and moisture difference in the direction of air flow increased and as the air flow rate increased, the drying rate increased and moisture differences decreased. In tunnel dryer, drying through bottom of the tray had large effect on drying rate and the effect was more significant when the drying temperature increased. As air flow rate increased, the difference of drying rates between tunnel and cabinet drying increased and drying rate of tunnel of drying was higher. The drying simulation model could estimate moisture content in tunnel more precisely by using modified effective moisture diffusion coefficient for air flow rate.