• Archives of Pharmacal Research
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• v.21 no.4
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• pp.378-384
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• 1998

The processing pathway of G-proteins and Ras family proteins includes the isoprenylation of the cysteine residue, followed by proteolysis of three terminal residues and .alpha.-carboxyl methyl esterification of the cysteine residue. Farnesylcysteine methyltransferase (FCMT) activity is responsible for the methylation reaction which play a role in the membrane attachment of a variety of cellular proteins. Four kinds of Ras protein (c-Ha-ras, c-N-Ras, c-Ki-Ras, pan-Ras) expression were detected in adenocarcinoma of human tissue by immunohistochemical method, and hematoxylin and eosin staining. The level of Ras protein in human stomach tumor tissues was much higher than in normal and peritumoral regions of the same biopsy samples. The FCMT activities of each cellular fractions were high in mitochondrial fraction followed by microsomal fraction, whole homogenate and cytosolic fraction. The inhibitory effect on FCMT activity on stomach tumor tissue was determined after treatment with 0.25 $\mu\textrm{M}$ of S-adenosyl-$_L$-homocysteine. S-adenosyl-$_L$-homocysteine inhibited FCMT activity from 11.2% to 30.5%. These results suggested that FCMT might be involved in Ras proteins activity.

• Journal of the Korean Chemical Society
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• v.66 no.5
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• pp.349-361
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• 2022

Because hydrotreating (HDT) of FCC gasoline is one of the important processes used to prepare such gasoline for blending, the development of a catalyst for this process is of great interest. Currently, the industrial HDT of FCC gasoline consists of two stages and the creation of a new catalyst for one-stage HDT will make this process more efficient. Recently, our group has developed the CoMo/Al₂O₃-ASA catalyst and studied the influence of Si/Al ratio on the target reactions of HDT process. Despite the high selectivity and activity, the catalyst with ASA is not applicable in industry because of its low strength. The present work moves forward to study the influence of the ASA content in the catalyst support and clarify the possibility to develop the catalyst that combines high activity and selectivity in HDT reactions with successful performance. Here we show that the CoMo catalyst with ASA/Al₂O₃ molar ratio 1/1 in the support is the best combination for FCC gasoline hydrotreatment due to exceptional properties of the catalyst composition.

• Molecules and Cells
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• v.43 no.10
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• pp.889-897
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• 2020

K-RAS is frequently mutated in human lung adenocarcinomas (ADCs), and the p53 pathway plays a central role in cellular defense against oncogenic K-RAS mutation. However, in mouse lung cancer models, oncogenic K-Ras mutation alone can induce ADCs without p53 mutation, and loss of p53 does not have a significant impact on early K-Ras-induced lung tumorigenesis. These results raise the question of how K-Ras-activated cells evade oncogene surveillance mechanisms and develop into lung ADCs. RUNX3 plays a key role at the restriction (R)-point, which governs multiple tumor suppressor pathways including the p14^ARF-p53 pathway. In this study, we found that K-Ras activation in a very limited number of cells, alone or in combination with p53 inactivation, failed to induce any pathologic lesions for up to 1 year. By contrast, when Runx3 was inactivated and K-Ras was activated by the same targeting method, lung ADCs and other tumors were rapidly induced. In a urethane-induced mouse lung tumor model that recapitulates the features of K-RAS-driven human lung tumors, Runx3 was inactivated in both adenomas (ADs) and ADCs, whereas K-Ras was activated only in ADCs. Together, these results demonstrate that the R-point-associated oncogene surveillance mechanism is abrogated by Runx3 inactivation in AD cells and these cells cannot defend against K-Ras activation, resulting in the transition from AD to ADC. Therefore, K-Ras-activated lung epithelial cells do not evade oncogene surveillance mechanisms; instead, they are selected if they occur in AD cells in which Runx3 has been inactivated.

• Journal of the Korean GEO-environmental Society
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• v.17 no.9
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• pp.21-28
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• 2016

The purpose of this study is to compare the three areas that each estimated by using three different river topographic maps. For construction of river topographic maps, the data used in this study are ASTER, SRTM and a 1:5,000 scale digital map data sets in 14 streams of the Cheongdo-gun and Uiseong-gun. HEC-GeoRAS, RAS Mapper, and RiverCAD model are applied for the flooding area analysis using observed data and design rainfalls. The result of analysis is to compare observed flooding area based on the flood plain maps with estimated inundation area by hydraulic models and constructed river topographic maps. The results of this study are as follows; Flooding area by HEC-GeoRAS model is similar to the inundation area of flood plain map and appears in order of RAS Mapper, and RiverCAD model in all watersheds. Flood inundation area by SRTM DEM is similar to the result of 1:5,000 scale digital map in all watersheds and all analysis models. The SRTM DEM shows the most similarity to the digital map than ASTER DEM in all of the watershed scale and analysis models. HEC-GeoRAS and RiverCAD model are efficient models for flood inundation analysis in small watershed and HEC-GeoRAS and Ras Mapper model are efficient in medium to large watershed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.4
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• pp.726-733
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• 2016

Recently, the flood frequency and magnitude have increased due to heavy rainfall. Considering the present condition, a flood risk map has been published in many countries to raise awareness about flood damage to people. A flood inundation analysis model, which is used to publish the flood risk map, can be classified as river and inland inundation models according to the inundation cause. Although a variety of flood inundation analysis models are utilized both domestically and overseas, their usability is limited by the expensive price, except for the HEC-RAS model developed by U.S. Army Corps of Engineers (USACE). In the situation, the USACE has developed a 2-D HEC-RAS model that can be linked to the existing 1-D model. This model has been released as a beta version under the name, HEC-RAS 5.0. In this study, the HEC-RAS 5.0 model's features, usability, applicability, and accuracy were evaluated by comparing the performances on Gokgyo-cheon with the FLUMEN model, which is used for domestic flood risk mapping. The results of this study will contribute to river inundation analysis in many different ways after the HEC-RAS 5.0 model is stabilized.

• Journal of Music and Human Behavior
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• v.12 no.2
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• pp.19-36
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• 2015

The purpose of this study was to examine the effects of rhythmic auditory stimulation (RAS) on gait parameters, with and without the presence of a melody, for adolescents with traumatic brain injury (TBI). Three adolescents with TBI received a total of ten individual RAS training sessions. At pre and posttest, spatiotemporal parameters including cadence, velocity and kinematic parameters were measured using the VICON 370 Motion Analysis System. The results showed no significant difference in gait velocity between the two conditions, thus the presence of the melody condition did not impact the outcome of RAS gait training. On the other hand, all participants showed improvement in gait function after RAS training. The cadence, velocity, stride length, and symmetry were increased and the stride time was reduced after training. The motion analysis demonstrated that the movement patterns of hip and knee joints improved, as they were more similar to normal gait, which indicates that the walkings tance became more stable. The research findings indicate that rhythm is the primary factor in mediating gait functions via RAS training. This study also supports that RAS training can effectively improve the gait function for adolescents with TBI.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• v.28 no.1
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• pp.245-259
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• 1998

Cellular transforming genes have been identified in a number of different tumor cell lines and tumor types. A significant number of these oncogenes belong to the ras gene family. The ras gene family consists of three closely related genes:H-ras, K-ras and N-ras which code for a related 21 kDa protein. Mutations in codon 12, 13 and 61 of one of the three ras genes convert these genes into acute oncogenes. The presence of H-ras gene mutations has important prognostic implications in various tumors. Each genomic DNA was isolated from tumors induced by implantation with DMBA, or by treatment with DMBA -implantation/irradiation. When genome DNA was transfected into NIH 3T3 cells and investigated by two-step PCR-RFLP, the fOllowing results were concluded: 1. Transformation foci developed in two groups when the genome DNA of two experimental groups were transfected into NIH 3T3 cells. 2. Transformation efficiency was 0.01-0.02 foci/㎍DNA in the experimental group with the DMBA-implantation, 0.01-0.03 foci/㎍lgDNA in the experimental group with the DMBA-implantation/irradiation according to results of transfection assay. 3. When the point mutation of H-ras gene was investigated by a two-step PCR-RFLP, there was 13.9％ (5/36) in the experimental group with the DMBA implantation, 15.4 ％ (6/39) in the experimental group with the DMBA -implantation/irradiation. 4. The point mutation in codon 12 and 61 of H-ras was 5.6％(2/36) and 8.3％(3/36) in the experimental group with the DMBA implantation. 5. The point mutation in codon 12 and 61 of H-ras gene was 7.7％(3/39) in the experimental group with the DMBA -implantation/irradiation.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.1
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• pp.119-124
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• 1994

Experiments were performed to determine age-associated changes in ornithine decarboxylase (ODC) gene and Ha-ras cellular oncogene expression in tissues of female rats. In the kidney, ODC mRNA levels did not show age-associated changes, while ODC enzyme activities were decreased with advancing age from 3 to 10 months. These results suggest that post-transcriptional mechanism (s) are involved in the age-dependent decrease in renal ODC enzyme activity. In addition, we found no correlation between testosterone-induced renal ODC expression and DNA methylation pattern. Ha-ras mRNA levels in brain decreased as animals aged from 3 to 6 months, while renal Ha-ras mRNA levels were not influenced by age. Results demonstrate the age-dependent expression of Ha-ras in a tissue-specific manner.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2001.10a
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• pp.314-318
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• 2001

The analysis of the spatial extent of flood inundation is important for flood mitigation. Geographic Information System (GIS) has advantage of analyzing spatial distributed data. Hydrologic Engineering Center's River Analsysis System(HEC-RAS) with HEC-GeoRAS was used to analyze flood inundation. HEC-GeoRAS, which is an ArcView GIS extension designed to process geospatial data for HEC-RAS, is a useful tool for storing, managing, analyzing, and displaying spatially distributed data. Rational formula and 24-hr duration probability precipitation data of Suwon meteorological station were used to estimate the flood runoff. And water profiles were calculated using the HEC-RAS model with HEC-GeoRAS. The flooded region is 8.24ha when 50-yr probability precipitation was applied and 8.8ha when 100-yr was applied to Bahlan study watershed which is located in Whasung county, Kyunggi province, having an area of $29.79km^{2}$.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.2699-2701
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• 2013

The RAS family genes encode small GTP-binding cytoplasmic proteins. Activated KRAS engages multiple effector pathways, notably the RAF-mitogen-activated protein kinase, phosphoinositide-3-kinase (PI3K) and RalGDS pathways. In the clinical field, K-ras oncogene activation is frequently found in human cancers and thus may serve as a potential diagnostic marker for cancer cells in circulation. This mini-review aims to summarise information on Ras-induced oncogenesis and the clinical significance of K-ras.