• Journal of Microbiology and Biotechnology
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• 제26권1호
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• pp.89-98
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• 2016

Endophytes play an important role in the growth and development of the host. However, the study of endophytes is mostly focused on plants, and reports on bacteria associated with fungi are relatively rare. We studied the bacteria associated with fruiting bodies of Tricholoma matsutake picked from seven main T. matsutake-producing areas in Sichuan, China, by barcoded pyrosequencing. About 8,272 reads were obtained per sample, representing 40 phyla, 103 classes, and 495 genera of bacteria and archaea, and 361-797 operational taxonomic units were observed at a 97% similarity level. The bacterial community was always both more abundant and more diverse than the archaeal community. UniFrac analysis showed there were some difference of bacterial communities among the samples sites. Three bacterial phyla, Proteobacteria, Bacteroidetes, and Firmicutes, were dominant in all samples. Correlation analysis showed there was a significant correlation between some soil properties and bacterial community associated with T. matsutake. This study demonstrated that the bacteria associated with T. matsutake fruiting bodies were diversified. Among these bacteria, we may find some strains that can promote the growth of T. matsutake.

• 환경영향평가
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• 제14권4호
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• pp.165-177
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• 2005

This study was carried out to offer the basic methodology of the system and model to objectively assess the natural ecosystem for environmentally friendly land conservation and present the alternative plan on establishing the environmental policy. The results of this study were as follows. We selected four assessment factors associated with biotic, abiotic, qualitative, and functional factors. Also, there were extracted fifty-six indicators including density, total nitrogen, hemeroby degree, and goods production. The assessment factor showed that biotic one was very important. The importance of indicators were analyzed that rare and endangered plant was important in biotic factor, in case of abiotic, qualitative, and functional factors, organic matter, landscape diversity, and conservation of ecosystem were greatly important. The results of factor analysis on the characteristics of indicators, classified biotic factor into six factors including a structural one, abiotic factor as a soil and physical one, qualitative factor as five ones including hierarchical one, and functional factor as public and conservational one. In the results of analysis on assessment model, R-square of biotic factor was 51.7%, those of abiotic, qualitative, and functional one were each 58.4%, 44.2%, and 39.3%, and statistical problem was no existence. In future, to develop the assessement model and methodology of sustainable natural ecosystem, we will have to achieve the integrated model and grouping by assessment factor.

• The Korean Journal of Ecology
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• 제19권5호
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• pp.431-451
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• 1996

The present studwas undertaken to classify and describe the riverside vegetation around Hanchon (36°27’-36°51’N, 128°15’-128°37’E), a tributary in the upper stream area of Nak-tong River, South Korea by methods of the ZM school of phytosociology. As a result the vegetation was divided into twenty three associations and communities belonging to ten classes. Reflecting various human impacts in the past, the associations and communities of the Artemisietea principis were most plentiful there. The vegetation units obtained in the present study were as follows: A. forest vegetation A-1 Fagetea crenatae:Zelkova serrata community, Larix leptolepis community; B. Shrub vegetation B-1 Salicetea sachalinensis:Salicetum gracilistylae, Salicetum graciliglandis assoc. nov., B-2 Rosetea sachalinensis:Salicetum gracilistylae, Salicetum graciliglandis assoc. nov., B-2 Rosetea multiflorae:Stephanandra incisa community, Lycium chinense community; C. Grassland vegetation C-1 Lemnetea minoris:Spirodela polyrhiza-Lemna paucicostata community, C-2 Bidentetea tripartiti:Polygonetum thunbergii, Polygonetum sieboldii-hydropiperis, Panico Polygonetum hydropiperis, Pharagmitetea :Oenantho- Phalaridetum arundinaceae, Phragmitetum japonicae, Miscanthetum sacchariflori, Phragmites australis community, C-4 Artemisietea principis:Artemisia princeps community, Impatiens textori community, Lactuco indicae-Humuletum japonicae, Pueraria lobata-Humulus japonicus community, Pennisetum alopecuroides community, C-5 chenopodietea:Echinochloa crus-galli var. orizicola community, Digitaria adsecendens community, Polygonum nodosum community, C-6 Miscanthetea sinensis: Miscanthus sinensis community, C-7 Plantaginetea:Eragrotis multicaulis-Plantago asiatica community. It is inferred that the endemic vegetation units in the riverside vegetation of Korea are very rare, because most of the vegetation units obtained in the present study are common with those of Japan. Among the above vegetation units, the Salicetum graciliglandis was established as a new association. On the other hand, the associations and communities of classes characterizing the riverside vegetation increased with a decrease of human impacts from the downstream to the upstream in Hanchon, while the number of the naturalized plants was the reverse. Also based on the present phytosociological work, the relation between the vegetation units and their environmental conditions and the restoration ecology and nature conservation of the riverside vegetation were discussed here in detail.

• 한국환경복원기술학회지
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• 제23권3호
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• pp.45-62
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• 2020

This study is to present the basic data for the conservation and management of natural resources of National Park by understanding the ecological characteristics of special protected areas located in Tohamsan District, Gyeongju National Park, South Korea. The numbers of vascular plants were summarized as 106 taxa including 47 families, 79 genera, 96 species, 1 subspecies, 7 varieties and 2 forms. For status by sites, there were 59, 50 and 55 taxa in A area(Pedicularis ishidoyana), B area(Millettia japonica) and C area(Cypripedium macranthos), respectively. In the dominant species, the tree layer was Quercus serrata(A area), Platycarya strobilacea and Pinus koraiensis(B area) and Fraxinus rhynchophylla(C area), the subtree layer was Carpinus cordata and Quercus serrata(A area), Pinus koraiensis and Castanea crenata(B area) and Fraxinus rhynchophylla(C area), the 1^st shrub layer was Toxicodendron trichocarpum and Rhododendron schlippenbachii(A area), Lindera erythrocarpa and Styrax japonicus(B area) and Fraxinus rhynchophylla and Lindera erythrocarpa(C area), 2^nd shrub layer was Styrax obassis and Lespedeza maximowiczii(A area), Lespedeza maximowiczii and Rhododendron mucronulatum(B area) and Lindera erythrocarpa(C area), the herbaceous layer was Athyrium yokoscense, Dryopteris chinensis, Dryopteris lacera and Lindera obtusiloba(A area), Athyrium yokoscense, Millettia japonica, Carex humilis Leyss. var. nana and Carex ciliato-marginata(B area) and Hydrangea macrophylla (Thunb.) Ser. subsp. serrata, Ajuga spectabilis and Oplismenus undulatifolius(C area).

• 식물조직배양학회지
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• 제27권4호
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• pp.259-268
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• 2000

Senescence is a sequence of biochemical and physiological events that lead to death of a cell, organ, or whole organism. Senescence is now clearly regarded as a genetically determined and evolutionarilly acquired developmental process comprising the final stage of development. However, in spite of the biological and practical importance, genetic mechanism of senescence has been very limited. Through forward and reverse genetic approaches, we are trying to reveal the molecular and genetic mechanism of senescence in plants, employing leaf organs of Arabidopsis as a model system. Using forward genetic approach, we have initially isolated several delayed senescence mutants either from T-DNA insertional lines or chemical-mutagenized lines. In the case of ore 4 and ore 9 mutants, the mutated genes were identified. The recent progress on characterization of mutants and identification of the mutated genes will be reported. We are also screening mutations from other various sources of mutant pools, such as activation tagging lines and promoter trap lines. Two dominant senescence-delayed mutants were isolated from the activation tagging pool. Cloning of the genes responsible for this phenotype is in progress. For reverse genetic approach, the genes that induced during leaf senescence were first isolated by differential screening method. We are currently using PCR-based suppression subtractive hybridization, designed to enrich a cDNA library for rare differentially expressed transcripts. Using this method, we have identified over 35 new sequences that are upregulated at leaf senescence stage. We are investigating the function of these novel genes by systemically generating antisense lines.

• 한국물환경학회지
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• 제24권2호
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• pp.206-213
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• 2008

Fish fauna and community structure were investigated at 2003, 2004 and 2006 in Lake Paldang and its inflows. In this survey, we collected 54 species belong to 14 families. Family Cyprinidae take 51.9% (28 species), Cobitidae and Gobiidae occupied 7.4% (each 4 species), respectively. E. erythropterus (11.8%) was dominant species and L. macrochirus (10.2%), designated as an ecosystem-invasive alien fish species by Ministry of Environment, M. yaluensis (9.1%), Z. platypus (7.7%) and R. brunneus (6.7%) were dominated in turn. H. molitrix, C. lutheri, P. altivelis, S. microdorsalis, L. costata. S. gracilis majimae, A. rivularis, P. koreanus. S. scherzeri and O. platycephala were rare species (less than 0.1% in relative abundance). In-lake area, 36 species of fishes belonging to 11 families were collected, and 48 species of 13 families were from inflows. The number of Korean endemic species were 19 species (35.2%), and 4 kinds of exotic species (7.4%), that is, C. cuvieri, H. molitrix, L. macrochirus and M. salmoides were found at this survey area. It was revealed by the analysis of fish community that diversity and richness indices were prominent at tributaries, and dominance index was high at the main body of Lake Paldang. Fish fauna showed rapid decline after dam construction which make the mid-Han river systems to lentic ecosystem, so many of meander-riffle fishes were disappeared dramatically. But after 1990s when installation of small scale wastewater treatment plants started in watershed, the water quality of small tributaries was improved. And also, expansion of the natural macrophytic vegetation of littoral zone should contribute to the development of diverse fish fauna in Lake Paldang.

• 생약학회지
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• 제38권2호통권149호
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• pp.157-163
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• 2007

Trillium tschonoskii Maxim. is a kind of 64 endangered plant species designated by the Ministry of Environment in Korea. It is very a rare native plant throughout the country. Therefore, this study was conducted to investigate the native T. tschonoskii in Ulleung Island for their distribution and ecological characteristics. They were mostly distributed valley, mid-slope of the mountain from 186 to 458 m altitude. They had a little low light conditions as they faced west, north west, south west, south-south west, north-north east and east-east north. They were provided with slightly humid condition. Aerial humidities of these quadrates were variable as 21.5-71%. They were located on the variable slopes of 5 to 43 degrees. Soil humidity was slightly dry. Native sites were semi-shaded because tree layer such as Tilia insularis, Zelkova serrata, okamotoanum etc.and sub-tall tree layer such as Ligustrum foliosum, Comellia japonica, Tilia insularis, Callicarpa japonica and so on were grown near by. Woody plants such as A. Okamotoanum, Sorbus commixta, Fagus japonica var. multinervis etc. were frequently observed. These environmental conditions were suitable that T. tschonoskii grows up naturally because of high Percentage as 52.78% of blooming individuals in this study. These native sites were classified with three communities, T. tschonoskii-Majanthemum dilatatum community, T. tschonoskii-Anthriscus sylvestris community and T. tschonoskii-Hepatica maxima community in accordance with altitude and aerial humidity. The best way ffr the preservation of T. tschonoskii habitats and maintainance of its recent population is maintained in recent environmental condition and ecological condition without any management by human-being.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2004년도 춘계학술대회 논문집
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• pp.296-302
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• 2004

After the Rio declaration on environment and development in 1992, developed countries are undertaking 'environmentally sustainable transportation (EST)' projects. To meet the needs for EST, current transportation policies in Korea are rapidly reforming and one of its concerns is modernizing and upgrading railway freight system. Planning new railroad construction projects is increasing and subsequent environmental impact assessment (EIA) demands improvements, especially in both the EIA and decision making systems. In this paper, we discuss the present status of EIA for railroad construction projects, especially, by analyzing the EIA documents accumulated for last six years. The EIA for railroad construction projects .accounts for only $4.9\%$ of total project EIAs during 1998-2003. However, the portion is gradually increasing. Major environmental concerns for EIA in railroad construction projects were geomorphological and ecological changes, protection of rare organisms, air pollution, water quality, wast management, noise, etc. We also compared the characteristics of environmental impacts of railroad construction with those of vehicle road construction. The result shows that railroad construction usually requires 3${\~}$4 times longer tunnels and bridges for a given length than vehicle road construction. In addition, the amounts of geomorphological and ecological changes (road-cutting, embankment, devegetation, etc.) in railroad construction were generally less than $40\%$ of those in vehicle road construction. In order to develop environmentally friendly railway systems, monitoring studies for environmental impacts of railroads such as habitat fragmentation and road kills, dispersal of alien plants, tunnelling effects on groundwater and vegetation, and noise impacts are highly required.

• 수산해양교육연구
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• 제25권2호
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• pp.433-447
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• 2013

In around of Guryongpo Pohang Port, a Guamegi production special zone is being established as modernized processing plants, cold storages, Guamegi research centers, or publicity centers are being built in a row with forming the tourism and culture streets as a start. Regarding the special Guamegi production zone, the local autonomous entities are promoting various projects as investing approximately 40 billion won as its budget; however, it is still very rare to elevate the level of Guamegi, the special product, and make investment plans to create a brand for it. To make a special product brand cannot be realized just by building the Guamegi industrialization processing complex, and mere publicity also does not guarantee sustainable development. It can be realized only when the product's taste or hygiene satisfies the people's needs. Therefore, this study examines the changes of Guamegi-related business and the strategies to develop Guamegi industry in order to find ways to manufacture Guamegi and cultivate specialized manpower for processing to make a special brand with Guamegi. Regarding the Guamegi business and the cultivation of specialized manpower for Guamegi, this study conducted a survey to 377 persons including the business owners, employees, or civil servants related with Guamegi. It is needed to install educational institutions to cultivate specialized manpower for Guamegi and also operate various programs so as to conduct education to cultivate specialized manpower for Guamegi. And it is necessary to elevate the quality and sanitation of Guamegi and create a brand by inducing a specialized license system that can help those involved in Guamegi business have pride.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 제10차 국제학술회의 및 추계정기 학술발표회
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• pp.2-3
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• 2003

A method for rapid propagation of mature Jack fruit was developed. Four types of explants (mature embryos, apical meristems of young seedlings, apices from mature plants and nodal segments) were used. It has been found 88% of young apical meristems produced shoots in Campbell and Durzan (CD) medium compared to 60% in Murashige and Skoog (MS) medium. Only 1/3 of them produced multiple shoots. Shoot initiation from nodal segments was very rare. Mature apices produced callus. Although removal of the sheathing cover around mature buds enhanced the shoot initiation but success rate was low in growth regulator free medium. Embryos respond to the CD medium but not to the MS medium. Embryos from seeds soaked in water for 24 hours produced shoots after 8 weeks of incubation and the success rate was 70% while embryos from dry seeds only produced roots. There was no significant effect of cold storage (refrigeration) for 7 days on shoot initiation from mature embryos (65%) but the ability for shoot induction declines with storage time (55% after 21 days of cold storage). Mature axillary buds were established in Modified Campbell and Durzan (CD) medium supplemented with 0.5mg/1 and IBA. There was a significant difference in the growth performance of shoots according to the period of the year in which explants were collected. Highest (60%) was observed in November-January period. It was only 30% when the explants were collected in February-April or May-July and decreased to 20% in August-October. The shoots produced in November-January showed a higher vigor than those produced in other months. Since Jak fruit show seasonal changes in fruit bearing and shedding of leaves, it can be suggested that the difference in growth performances of tissues cultured in artificial culture media would have been affected by endogenous rhythms.