• Parasites, Hosts and Diseases
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• 제45권1호
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• pp.65-68
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• 2007

The application of Giemsa technique to stain compressed diaphragm samples obtained from rodents experimentally infected with Trichinella spiralis is described. Diaphragm samples from rats heavily infected with 20 muscle larvae per gram of body weight(20 ML/gbw) were cut into several pieces and stained with Giemsa; on the other hand, whole diaphragms from slightly infected mice(1 ML/gbw) were also stained with Giemsa. Besides, muscle samples were also stained with Giemsa. Observation at 10 $\times$ magnification revealed that both ML and nurse cells(NC) look as bluish structures clearly contrasting with the pinkish color of the non-infected muscle fibers. NC in the diaphragms of mice could be easily observed at naked eye as blue points contrasting with the pink surrounding areas formed by the non-infected muscle fibers. Among NC observed in the diaphragms of rats infected with 20 ML/gbw, 4.4% was multiple infection. These findings were confirmed in sectioned and hematoxylin-eosin stained specimens. This data could be usefulness for a rapid diagnosis of trichinellosis in post-mortem mammals without magnification procedures.

• Biomolecules & Therapeutics
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• 제26권4호
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• pp.368-373
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• 2018

Rapid eye movement (REM) sleep has an essential role in the process of learning and memory in the hippocampus. It has been reported that linalool, a major component of Lavandula angustifolia, has antioxidant, anti-inflammatory, and neuroprotective effects, along with other effects. However, the effect of linalool on the cognitive impairment and behavioral alterations that are induced by REM-sleep deprivation has not yet been elucidated. Several studies have reported that REM-sleep deprivation-induced memory deficits provide a well-known model of behavioral alterations. In the present study, we examined whether linalool elicited an anti-stress effect, reversing the behavioral alterations observed following REM-sleep deprivation in mice. Furthermore, we investigated the underlying mechanism of the effect of linalool. Spatial memory and learning memory were assessed through Y maze and passive avoidance tests, respectively, and the forced swimming test was used to evaluate anti-stress activity. The mechanisms through which linalool improves memory loss and behavioral alterations in sleep-deprived mice appeared to be through an increase in the serotonin levels. Linalool significantly ameliorated the spatial and learning memory deficits, and stress activity observed in sleep-deprived animals. Moreover, linalool led to serotonin release, and cortisol level reduction. Our findings suggest that linalool has beneficial effects on the memory loss and behavioral alterations induced by REM-sleep deprivation through the regulation of serotonin levels.

• 대한임상검사과학회지
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• 제39권3호
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• pp.264-270
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• 2007

Obstructive sleep apnea syndrome (OSAS) is defined by sleep apnea with decreased oxygen saturation, excessive snoring with daytime sleepiness, and frequent awakening during the night time sleep. The present study was performed to investigate how apnea-hypopnea, that possibly causes breathing disturbance during sleep, can affect sleep pattern in patients with OSAS. We included 115 patients (92 men, 23 women) who underwent a polysomnography from January 2006 to May 2007. As the frequency of sleep apnea-hypopnea increases, the proportion of non-rapid eye movement (REM) sleep (p<0.001), and stage I sleep (p<0.001) increased, while that of stage II sleep (p<0.001), stage III and IV sleep (p<0.01), and REM sleep (p<0.05) decreased. Furthermore, sleep apnea-hypopnea was closely correlated with REM sleep (r=0.314, p<0.001), stage I sleep (r=0.719, p<0.001), stage II sleep (p=-0.342, p<0.05), stage III and IV sleep (r=-0.414, p<0.001), and REM sleep (r=-0.342, p<0.05). Stage I sleep could account for the 51% of the variance of apnea-hyponea. Our study shows sleep apnea-hypopnea affects sleep pattern in pattern with OSAS significantly, and the change of stage I sleep is the most important factor in estimating the disturbance of sleep pattern.

• 한국측량학회지
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• 제33권6호
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• pp.547-555
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• 2015

The vegetation index is a crucial parameter in many biophysical studies of vegetation, and is also a valuable content in ecological processes researching. The OVIs (Optical Vegetation Index) that of using multispectral and hyperspectral data have been widely investigated in the literature, while the RVI (Radar Vegetation Index) that of considering volume scattering measurement has been paid relatively little attention. Also, there was only some efforts have been put to fuse the OVI with the RVI as an integrated vegetation index. To address this issue, this paper presents a novel FVI (Fusion Vegetation Index) that uses multispectral and full-PolSAR (Polarimetric Synthetic Aperture Radar) data. By fusing a NDVI (Normalized Difference Vegetation Index) of RapidEye and an RVI of C-band Radarsat-2, we demonstrated that the proposed FVI has higher separability in different vegetation types than only with OVI and RVI. Also, the experimental results show that the proposed index not only has information on the vegetation greenness of the NDVI, but also has information on the canopy structure of the RVI. Based on this preliminary result, since the vegetation monitoring is more detailed, it could be possible in various application fields; this synergistic FVI will be further developed in the future.

• Biomolecules & Therapeutics
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• 제25권4호
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• pp.390-395
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• 2017

The present study investigated the sedative effects of Sophora flavescens (SF) and its bioactive compound, matrine through performing locomotor activity test and the electroencephalography (EEG) analysis in the rat. The underlying neural mechanism of their beneficial effects was determined by assessing c-Fos immunoreactivity and serotonin (5-HT) in the brain utilizing immunohistochemical method and enzyme-linked immunosorbent assay. The results showed that SF and matrine administration had an effect on normalization of caffeine-induced hyperactivity and promoting a shift toward non-rapid eye movement (NREM) sleep. c-Fos-immunoreactivity and 5-HT level in the ventrolateral preoptic nucleus (VLPO), a sleep promoting region, were increased in the both SF and matrine-injected groups. In conclusion, SF and its bioactive compound, matrine alleviated caffeine-induced hyperactivity and promoted NREM sleep by activating VLPO neurons and modulating serotonergic transmission. It is suggested that SF might be a useful natural alternatives for hypnotic medicine.

• Journal of mucopolysaccharidosis and rare diseases
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• 제1권2호
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• pp.35-39
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• 2015

Sleep problems occur frequently among patients with Prader-Willi syndrome (PWS). The most common problem is excessive daytime sleepiness (EDS) that are closely related to of sleep-related breathing disorder (SRBD) such as obstructive sleep apnea (OSA) and congenital hypoventilation syndrome. Obesity, craniofacial dysmorphism and muscular hypotonia of patients with PWS may increase the risk of SRBD. Sleep apneas can interrupt the continuity of sleep, and these disruptions result in a decrease in both the quality and quantity of sleep. In addition to SRBD, other sleep disorders have been reported, such as hypersomnia, a primary abnormality of the rapid eye movement (REM) sleep and narcolepsy traits at sleep onset REM sleep. Patients with PWS have intrinsic abnormalities of sleep-wake cycles due to hypothalamic dysfunction. The treatment of EDS and other sleep disorders in PWS are similar to standard treatments. Correction of sleep hygiene such as sufficient amount of sleep, maintenance of regular sleep-wake rhythm, and planned naps are important. After comprehensive evaluation of sleep disturbances, CPAP or surgery should be recommended for treatment of SRBD. Remaining EDS or narcolepsy-like syndrome are controlled by stimulant medication. Bright light therapy might be beneficial for disturbed circadian sleep-wake rhythm caused by hypothalamic dysfunction.

• 전기전자학회논문지
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• 제6권1호
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• pp.10-20
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• 2002

눈의 홍채는 사람마다 독특한 문양을 갖고 있다. 홍채를 이용한 인식 시스템은 지문 인식 시스템보다 신뢰성이 더 높은 것으로 평가되고 있다. 본 논문은 비밀번호나 도장을 사용하지 않고도 간단히 눈동자만으로 신원을 확인할 수 있는 신분 인식 시스템을 개발하고자 하는 것이다. 홍채 인식 알고리즘은 우선 영상에서 홍채부분을 인식 분리한다. 홍채 영상이 구해지면 특이 성질들을 추출하게 된다. 특이 성질들은 웨이블렛 변환을 이용하여 구한다. 영상에서 대역별 공간별 특징이 추출되면 홍채 코드가 만들어진다. 만들어진 코드는 수정된 CPN 신경망에 입력되어 신원을 확인하게 된다. 웨이블렛 변환의 특성을 이용하여 대역별로 코드를 작성한다면 이를 이용한 인식도 저주파 대역에서 고주파 대역의 방향으로 계층적으로 수행할 수 있다. 이는 인식 시간을 최대한 단축할 수 있게 할 것이다.

• 농촌계획
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• 제10권2호
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• pp.51-60
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• 2004

Through the fast economic growth since the 1960s, Korea has experienced various problems on land such as urban sprawl, the rapid increase in land prices, land speculation, privatization of the betterment, and so on. To prevent such problems and to enhance harmonious development and conservation, the National Land Planning and Use Act(NLPUA) was established in 2003. The NLPUA which was revised and combined two existing planning laws i.e. the National Land Use Management Act and Urban Planning Act, has an eye to preventing disorderly spatial development, to pursuing environmentally friendly spatial planning, and to following up planned development in non-urbanized area like rural area. This study aims to discuss what should be considered the pending issues after the application of the NLPUA in rural area. On the basis of reviewing the NLPUA in a viewpoint of the rural planning, this study suggests some improvement policy such as considering various rural conditions, securing excellent agricultural land, applying the District Plan II system effectively, reorganizing the planning administrative, and so on.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2000년도 제15차 학술회의논문집
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• pp.493-493
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• 2000

Multi-valued decision making for transitional stochastic events was newly derived based on conditional probability of database. The two values (on-off) decision making method without transition had been proposed by one of the author in a previous work for a purpose of realizing human on-off decision making. The current method is an extension of the previous on-off decision making. By combining the conditional probability and the transitional probability, the closed form of the algorithm for the multi-valued transitional decision making was derived. The proposed multi-valued decision making was successfully applied to the determination of the five levels of the vigilance of a subject during the EEG recording; awake stage, drowsy stage and sleeping stages (stage 1, stage 2/3, REM (rapid eye movement)). The method for determining the vigilance level can be directly usable for the two purposes; selection of awake EEG segments for automatic EEG interpretation, and determination of sleep stages through sleep EEG. The proposed multi-valued decision making with a mathematical background of the probability can be applicable widely, in industries and in medical fields for purposes of the multi-valued decision making.

• 농촌계획
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• 제21권1호
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• pp.19-28
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• 2015

As environmental concerns including climate change drive the strong regulations for car exhaust emissions, electric vehicles attract the public eye. The purpose of this study is to identify rural areas vulnerable for charging infrastructures based on the spatial distributions of the current gas stations and provide the target dissemination rates for promoting electric cars. In addition, we develop various scenarios for finding optimal way to expand the charging infrastructures through the administrative districts data including 11,677 gas stations, the number of whole national gas stations. Gas stations for charging infrastructures are randomly selected using the Monte Carlo Simulation (MCS) method. Evaluation criteria for vulnerability assessment include five considering the characteristic of rural areas. The optimal penetration rate is determined to 21% in rural areas considering dissemination efficiency. To reduce the vulnerability, the charging systems should be strategically installed in rural areas considering geographical characteristics and regional EV demands.