• Title/Summary/Keyword: Rapid methods

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Development of a ladder-shape melting temperature isothermal amplification (LMTIA) assay for detection of African swine fever virus (ASFV)

  • Wang, Yongzhen;Wang, Borui;Xu, Dandan;Zhang, Meng;Zhang, Xiaohua;Wang, Deguo
    • Journal of Veterinary Science
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    • v.23 no.4
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    • pp.51.1-51.10
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    • 2022
  • Background: Due to the unavailability of an effective vaccine or antiviral drug against the African swine fever virus (ASFV), rapid diagnosis methods are needed to prevent highly contagious African swine fever. Objectives: The objective of this study was to establish the ladder-shape melting temperature isothermal amplification (LMTIA) assay for the detection of ASFV. Methods: LMTIA primers were designed with the p72 gene of ASFV as the target, and plasmid pUC57 was used to clone the gene. The LMTIA reaction system was optimized with the plasmid as the positive control, and the performance of the LMTIA assay was compared with that of the commercial real-time polymerase chain reaction (PCR) kit in terms of sensitivity and detection rate using 200 serum samples. Results: Our results showed that the LMTIA assay could detect the 104 dilution of DNA extracted from the positive reference serum sample, which was the same as that of the commercial real-time PCR kit. The coincidence rate between the two assays was 100%. Conclusions: The LMTIA assay had high sensitivity, good detection, and simple operation. Thus, it is suitable for facilitating preliminary and cost-effective surveillance for the prevention and control of ASFV.

An Experimental Study on the Alkali-Silica Reaction of Crushed Stones (Part4: The Application of the JIS Rapid Test Method to the Several Domestic Reactive Aggregates) (쇄석골재의 알칼리-실리카 반응에 관한 실험적 연구 (제4보: 국내산 반응성 골재에 JIS 신속법 적용가능성))

  • 차태환;조원기;조일호;노재호;이양수;정재동;윤재환
    • Proceedings of the Korea Concrete Institute Conference
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    • 1994.10a
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    • pp.447-450
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    • 1994
  • The chemical method and mortar-bar method for identification of the susceptibility to Alkali-Aggregate Reaction (AAR) was established as KS method by referencing the ASTM methods. However, the chemical method requires skilled chemical engineers and aggregates are tested in very severe condition, and on the other hand, the mortar-bar method needs a long time of 3 or 6 months. Judging from this circumstance that the use of crushed stones are increased due to the shortage of natural aggregates, the development and standardization of a new rapid test method is considered essential. The purpose of this paper is to research for the possibility to apply the rapid method, instead of the chemical method and the mortar-bar method with using the several domestic crushed stones.

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Development of a Real-Time Control System for Rapid Prototyping (Rapid Prototyping을 위한 실시간 제어시스템 개발에 관한 연구)

  • Kang, Moon-Ho;Jeong, Kyung-Min;Park, Yoon-Chang
    • Proceedings of the KIEE Conference
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    • 1999.07b
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    • pp.927-929
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    • 1999
  • In this research a real-time control system was developed without program codings during control system designing procedures. On the Simulink window control system is designed in the form of block diagrams, program codes are produced automatically with the real time workshop package, then C-compiler compiles the program codes. With this automatic real-time program producing mechanism rapid prototyping is realized. To show effectiveness of the proposed system designing scheme a DSP-based DC motor speed control system was constructed and PI and Fuzzy control methods were implemented.

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A robust controller design for rapid thermal processing in semiconductor manufacturing

  • Choi, Byung-Wook;Choi, Seong-Gyu;Kim, Dong-Sung;Park, Jae-Hong
    • 제어로봇시스템학회:학술대회논문집
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    • 1995.10a
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    • pp.79-82
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    • 1995
  • The problem of temperature control for rapid thermal processing (RTP) in semiconductor manufacturing is discussed in this paper. Among sub=micron technologies for VLSI devices, reducing the junction depth of doped region is of great importance. This paper investigates existing methods for manufacturing wafers, focusing on the RPT which is considered to be good for formation of shallow junctions and performs the wafer fabrication operation in a single chamber of annealing, oxidation, chemical vapor deposition, etc., within a few minutes. In RTP for semiconductor manufacturing, accurate and uniform control of the wafer temperature is essential. In this paper, a robustr controller is designed using a recently developed optimization technique. The controller designed is then tested via computer simulation and compared with the other results.

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Effects of Re-vibration and Curing Temperature on the Physical Properties of Latex-Modified Concrete (진동가력과 양생온도가 라텍스개질 콘크리트에 미치는 영향)

  • 정원경;홍창우;이주형;윤경구
    • Proceedings of the Korea Concrete Institute Conference
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    • 2003.05a
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    • pp.799-804
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    • 2003
  • The purpose of this study was to investigate the effects of re-vibration and curing temperature onto the physical properties of latex-modified concrete with ordinary cement and rapid-setting cement, and thus to provide a guide line of re-vibration and curing conditions for good quality controls. The main experimental variables included two cement types(ordinary portland cement, rapid-setting cement), curing Temperature($10^{\circ}C$, $20^{\circ}C$, $30^{\circ}C$), re-vibration methods(continued, intermittent), and re-vibration times(initial setting, one day after mixing). The experimental results showed that the re-vibration affected little to the mechanical properties of LMC and RSLMC, while, the curing temperature a quite some. The early strength development was the highest at $20^{\circ}C$ curing temperature, and decreased at higher temperature. The permeability of concrete generally decreased with curing time. The rapid chloride permeability was a function of time and temperature. The chloride permeability of RSLMC was so small and negligible.

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Salvage rapid maxillary expansion for the relapse of maxillary transverse expansion after Le Fort I with parasagittal osteotomy

  • Lee, Hyun-Woo;Kim, Su-Jung;Kwon, Yong-Dae
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.41 no.2
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    • pp.97-101
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    • 2015
  • Maxillary transverse deficiency is one of the most common deformities among occlusal discrepancies. Typical surgical methods are segmental Le Fort I osteotomy and surgically-assisted rapid maxillary expansion (SARME). This patient underwent a parasagittal split with a Le Fort I osteotomy to correct transverse maxillary deficiency. During follow-up, early transverse relapse occurred and rapid maxillary expansion (RME) application with removal of the fixative plate on the constricted side was able to regain the dimension again. RME application may be appropriate salvage therapy for such a case.

MEDIUM-TERM LIVER BIOASSAY FOR RAPID DETECTION OF CARCINOGENS

  • Hasegawa, Ryohei;Shirai, Tomoyuki;Ito, Nobuyuki
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1993.04a
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    • pp.91-91
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    • 1993
  • The vast number of compounds which have been introduced into our environment in recent years is far beyond our capacity to assess for carcinogenicity using expensive, life-time carcinogenicity studies in rodents. Several in vitro short term assays which are very rapid and inexpensive have therefore been applied as mass screening methods. However, increasing evidence has indicated that mutagenicity results do not always correlate with long-term carcinogenicity. Therefore, the existence of discrepancies dictates the necessity of suitable in vivo rapid assay systems which could bridge the gap between mutagenicity and conventional long-term testing.

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A fast adaptive numerical solver for nonseparable elliptic partial differential equations

  • Lee, June-Yub
    • Journal of the Korean Society for Industrial and Applied Mathematics
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    • v.2 no.1
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    • pp.27-39
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    • 1998
  • We describe a fast numerical method for non-separable elliptic equations in self-adjoin form on irregular adaptive domains. One of the most successful results in numerical PDE is developing rapid elliptic solvers for separable EPDEs, for example, Fourier transformation methods for Poisson problem on a square, however, it is known that there is no rapid elliptic solvers capable of solving a general nonseparable problems. It is the purpose of this paper to present an iterative solver for linear EPDEs in self-adjoint form. The scheme discussed in this paper solves a given non-separable equation using a sequence of solutions of Poisson equations, therefore, the most important key for such a method is having a good Poison solver. High performance is achieved by using a fast high-order adaptive Poisson solver which requires only about 500 floating point operations per gridpoint in order to obtain machine precision for both the computed solution and its partial derivatives. A few numerical examples have been presented.

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Field Enhanced Rapid Thermal Process for Low Temperature Poly-Si TFTs Fabrications

  • Kim, Hyoung-June;Shin, Dong-Hoon
    • 한국정보디스플레이학회:학술대회논문집
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    • 2005.07a
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    • pp.665-667
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    • 2005
  • VIATRON TECHNOLOGIES has developed FE-RTP system that enables LTPS LCD and AMOLED manufacturers to produce poly-Si films at low cost, high throughput, and high yield. The system employs sequential heat treatment methods using temperature control and rapid thermal processor modules. The temperature control modules provide exceptionally uniform heating and cooling of the glass substrates to within ${\pm}2^a\;C$. The rapid thermal process that combines heating with field induction accelerates the treatment rates. The new FE-RTP system can process $730{\times}920mm$ glass substrates as thin as 0.4 mm. The uniform nature of poly-Si films produced by FE-RTP resulted in AMOLED panels with no laser-Muras. Furthermore, FE-RTP system also showed superior performances in other heat treatment processes involved in poly-Si TFT fabrications, such as dopant activation, gate oxide densification, hydrogenation, and pre-compaction.

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Current Status on Seismic Design/Evaluation of Metropolitan Rapid Transit System (도시철도 구조물의 내진설계 및 평가 현황조사)

  • Kim, Jong-Min;Kim, Jin-Ho;Lim, Nam-Hyoung;Kang, Young-Jong
    • Proceedings of the KSR Conference
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    • 2008.06a
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    • pp.220-223
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    • 2008
  • Many structural engineers believed that RC underground structures like subway system might not be seriously damaged during earthquakes, until the Great Hanshin earthquake(a.k.a Kobe earthquake). But there is only one domestic seismic code of rapid transit system that established by Ministry of Construction & Transportation in 2005. Therefore, to investigate of current status on seismic design and evaluation method of rapid transit system is essential to estimate seismic performance of subway structural systems. In this study, comparing domestic codes and seismic evaluation methods with foreign system is performed.

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