• Biomedical Science Letters
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• v.24 no.3
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• pp.196-205
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• 2018

Reactive oxygen species (ROS) generated from metabolic reactions cause oxidative DNA damage, which results in oxidative tissue injury. Therefore, there is an increasing demand in the intake of high antioxidant sources in order to maintain a healthy environment in cells. In this study, we investigated the antioxidant and anti-inflammatory activities of Malus domestica (apple), Pyrus communis L. (pear), Daucus carota L. (carrot), Brassica oleracea var. (broccoli), Brassica oleracea var. capitata (cabbage), and Raphanus sativus L. (radish) obtained from the local market. Since these are common fruits and vegetables that are widely consumed, we aimed to investigate their beneficial properties, placing particular emphasis on their antioxidant and anti-inflammatory properties. The samples were processed via an indirect heating method and their properties were compared to their raw forms. Based on DPPH and ABTS assays, processed samples showed better antioxidant activities when compared to raw samples and processed pear samples exhibited the best antioxidant activity. The anti-inflammatory activities of the samples were also investigated in LPS-treated RAW 264.7 cells. mRNA expression of pro-inflammatory mediators and cytokines (iNOS, COX-2, $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6) was assessed using RT-PCR. As expected, processed samples exhibited better iNOS inhibition when compared to their raw forms and processed broccoli and cabbage samples exhibited outstanding anti-inflammatory effects. The samples, up to 1 mg/mL concentration, did not exhibit cytotoxicity against RAW 264.7 cells as demonstrated by cell viability assays. Altogether, processed broccoli and cabbage samples exhibited the strongest anti-inflammatory properties.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.3
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• pp.242-250
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• 2016

Veterinary antibiotics (VAs) has been used to treat animal disease and to increase animal weight as growth promoter. However, abused usage of VAs can cause production of antibiotic resistance genes (ARGs) in the environment and additionally, residual of VAs in soil can be transferred into crops. Therefore, main objective of this research was to examine bioaccumulation of VAs in sprouts (red cabbage, Brassica Olearacea L. var. Capitata f. rubra and red radish, Raphanus sativus) with hydroponic method. Total of 7 VAs in 2 different classes of VAs (tetracyclcines: tetracycline, oxytetracycline, chlortetracycline, sulfonamides: sulfamethoxazole, sulfamethazine, sulfamethiazole, macrolides: tylosin) were evaluated and experiment was conducted with solid phase extraction (SPE)/high performance liquid chromatography tandem mass spectrometry (HPLC/MS/MS). Initial spiked concentration of 7 VAs was $5mg\;L^{-1}$ and cultivation period was 8 days. Result showed that growth of sprouts was inhibited about 23-27% when VAs was introduced. Amount of bioaccumulated VAs was also differed depending on class of VAs. The highest amount of bioaccmulated VAs was tetracycline and sulfamethoxazole in each class with a concentration of 4.05, $7.73mg\;kg^{-1}$ respectively. Calculated transfer ratio of VAs into crops was also ranged 0.38-54.27%. Overall, bioaccumulation of VAs in crops can be varied depending on crop species and class of VAs. However, further research should be conducted to verify bioaccumulation of VAs in crops in the soil environment.

• The Plant Pathology Journal
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• v.21 no.4
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• pp.369-376
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• 2005

Turnip mosaic virus (TuMV) is an infectious viral pathogen on the cruciferous crops, predominantly Chinese cabbage (Brassica campestris subsp. pekinensis) and radish (Raphanus sativus). On the basis of the symptom development in selective differential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines, the representative eight isolates of TuMV were divided into two major groups/or six types. Group I includes Th 1, Ca-ad7, and Cj-ca2-1 isolates, while group II includes the other isolates (rg-pfl, r 9-10, Rhcql-2, Stock and Mustard). According to the molecular phylogenetic analysis, these isolates, however, divided into two groups and two independent isolates. Phylogenetic analysis indicated that four isolates (Tu 1, r9-10, Stock and Rh-cql-2) formed a distinct phylogenetic group, and the other two isolates (Ca-ad7 and Cj-ca2-1) also formed another group. Mustard and rg-pfl isolates did not seem to have any relationship with these two groups. Taken together, these results indicated that virulence differentiation on host plants, molecular phylogenetic analysis of the nucleotide and the deduced amino acid of TuMV coat proteins did not show any relationship. The multi-resistant lines, Wonyae 20026 and BP058 in Chinese cabbage represent valuable genetic materials that can be used for crucifer breeding programs on TuMV resistance, but not in Korean radish.

• Parasites, Hosts and Diseases
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• v.21 no.1
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• pp.105-110
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• 1983

A survey on vegetables, which consisted of lettuce (Lactuca sativa) , young radish (Raphanus sapiwus), and chinese cabbage (Brassica pekinensis) collected from 6 markets in the Taegu city, was conducted for the discovery of helminth eggs and larvae, from July, 1982 to June, 1983. The results were compared with the data obtained from vegetables collected at the same markets by Lee (1969) and Choi and Lee (1972). Both sides of vegetable leaves were washed carefully with a hard brush, and the species of parasites and the mean number of parasites per 200g of vegetable were determined. When vegetable were examined, 4 species of parasite eggs (ascarid, Trichostrongylus, liver fluke, and hookworms) and 2 larvae (filariform and rhabditoid larvae) were found. Of the parasite discovered, ascarid egg was found to be highest (4.2%) , followed by hookworm egg (3.6%) . The mean number of ascarid egg per 200 grams of vegetable was 0.6 in young radishes, and 0.3 in both lettuces and and chinese cabbages. Similarly, the number of filariform larva of hookworm was 0.4 in young radishes, 0.3 in lettuces, and 0.1 in chinese cabbages, and the number of rhabditoid larva was 0.2 in young radishes, 0.1 in lettuces, and 0.05 in chinese cabbages. In the results obtained by Lee (1969) and Choi and Lee (1972) , the mean number of ascarid egg per 200 grams of vegetable was 7.5 in young radishes, 3.1 in lettuces, and 0.5 in chinese cabbages. By contrast, in the present study it was 0.6 in young radishes an 0.3 in both lettuces and chinese cabbages. These results suggest that there has been significant reduction in the incidence of parasite egg and larva on vegetable leaves during the past 10 years.

• Korean Journal of Plant Resources
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• v.14 no.2
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• pp.108-115
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• 2001

For determining the effect of extracts exudated from ten industrial plant resources on germination and early growth of shoot and root of radish the allelopathic compounds screened. Germination rate and seedling growth of radish differed depending on plant species and extract concentration. Lower levels of 1% extracts exudated from industrial plant resources promoted the rate of germination of radish while higher levels of 10% extract inhibited the rate of germination. Germination rate, shoot growth and root growth of radish in higher concentration of extract also differed depending on the plant species exudated and extract concentraton of industrial plant resources. Higher concentration of extracts from Xanthium strutnarium L. Angellica gigas Nakai, and Solanum nigrum L. were completely inhibited the germination while those from Cichorium intybus L., and Polygonatum officinale Allioni were not inhibited. Extracts exudated from Xanthium strutnarium L, Angellica gigas Nakai, and Solanum nigrum L. showed the strong inhibition of shoot and root growth of radish while those from Cichorium intybus L., and Polygonatum officinale Allioni did not show the bioactivity in shoot and root growth of radish..

• Korean Journal of Plant Resources
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• v.26 no.1
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• pp.19-25
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• 2013

This study focused on the usefulness of plant extracts for a city organic farm. Insecticidal activity of 8 plant species, plants crude extracts by ethanol and water, against third instar larvae (susceptible strain) of Plutella xylostella was evaluated in this study. Ethanol(25%) extracts of plant materials had better insecticidal activity than water extracts at the room temperature. Insecticidal activity were investigated by leaf disc spray method. Five plant extracts (Capsicum annuum, Chelidoniun majus, Leonurus sibiricus, Coptis japonica, Mentha piperascens) showed over 50% insecticidal activity to P. xylostella at the concentration of $1500mg{\cdot}L^{-1}$. Extract of C. annuum showed the highest insecticidal activity (83.3%) against P. xylostella. Phytotoxicity was not observed on Raphanus sativus and Brassica campestris with spray application. Seed germination and growth of two plants were not affected. Extract of C. japonica revealed 73.3% control efficacy against P. xylostella of R. sativus and showed 70% control efficacy against those of B. campestris, respectively, in plastic house. Insecticidal activity against various insects was observed in C. japonica extract as 87.4% for R. sativus and 74.3% for B. campestris in the field. These results suggested that extracts of C. annuum, C. japonica showed the highest insecticidal activity, and could be used as city farm insecticides for organic farming.

• Korean journal of applied entomology
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• v.23 no.1 s.58
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• pp.28-36
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• 1984

The relationship between ratio of virus diseases and the population of aphid vectors was studied by planting radishes(Rophanus sativus L. var. Chungsu gungjung) every 10 days and collecting aphids from April to November at Jeonju, Korea in 1982. Alate aphids were collected with yellow pan traps and virus infection ratios were checked with symptoms. The populations of flying aphids showed two peaks, one in the late May and one in the middle October in Jeonju and the population of aphid vectors also showed the same inclination. Of aphid vectors, Myzus persicae, Lipaphis erysimi, Brevicoryne brassicae, and Aphisgossypii were trapped and their percentages to total trapped aphids were $62.82\%(39,260/62,499)$ M. persicae was the most prevalent species and its percentages to aphid vectors were $82.53\%(32,401/39,260)$. High and sudden increase in virus infection ratio was found in spring while the trend in autumn was slow and low. A correlation coefficient between the population of virus vector aphid and virus infection ratio was $r=0.7414^*(\hat{Y}=8.1444+0.0551X)$ in spring and $r=0.9117^{**}(\hat{Y}=10.2590+0.463X)$ in autumn. The symptoms of radish virus diseases appeared approximately 15 days after virus vector aphids had attacked. Virus infection ratios were higher on plots where virus infected plants were reserved than on plots where they were removed.

• Korean Journal of Environmental Agriculture
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• v.9 no.2
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• pp.121-131
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• 1990

Four crop plants, such as green onion(Allium ascalonicum L.), radish(Raphanus sativus L.), potato(Solanum tubersum L.) and chinese cabbage(Brassica pekinensis Rupr), were grown in greenhouses to determine the accumulations of Cd and Zn absorbed by the plants grown on soils treated with municipal sewage sludge of different levels of 0, 22.5, 45.0 and 90.0 ton $ha^{-1}$ as soil dry weight. The result was analyzed to be comparable to the heavy metal content in the plant tissues among the crop species and the statistical characteristics were investigated to determin a possible relationship between causes of increased contents of Cd and Zn in plants and in soils. The results were as follows : 1. The Cd and Zn content in experimental crop plants increased with increase in quantity of treatment sludge. 2. The Cd and Zn content in roots of all experimental plants increased with sludge treatment amount in soil, in the following order : green onion>potato>chinese cabbage>radish. 3. The Cd content in leaves of crop plants were high in this order : green onion>radish>potato>chinese cabbage, and the Zn content in leaves of crop plants was high in this order. : potato>radish>chinese cabbage>green onion. 4. Concentrations of Cd in potato tubers, radish roots and chinese cabbage leaves ranged from 0.03 to 0.12 ppm. 5. Concentrations of Cd and Zn in plant tissues grown in sludge treated soil of 90.0 ton $ha^{-1}$ were 1.2 to 10 times and 0.4 to 2.3 times higher as compared with those absorbed from sludge-free soil, respectively. 6. The accumulation rates of Cd and Zn in sludge-treated plant tissue were almost constant except for green onion roots.

• Korean Journal of Environmental Agriculture
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• v.16 no.4
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• pp.347-355
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• 1997

For the selection of plants contained pesticidal active conpounds, 31 families 59 species of native plants were collected and biological activites of their crude extracts against brown planthopper (Nilaparvata lugens), Xanthomonas oryzae pv. oryzae and allelopathy were examined. Among the screened plants, the crude extracts from the leaves of Ricinus communis and Sophora angustifolia showed 100% and 82% of mortality on brown planthopper at the concentration of 1% (w/v) respectively. Mixed crude extracts of Sophora angustifolia root and Melia azedarach seed exhibited 128${\sim}$155% of synergistic effects on the mortality of brown planthopper. In case of fungicidal activity, the crude extracts from the leaves of 8 plants including Chrysanthemum indicum were inhibitory of the growth of Xanthomonas oryzae pv. oryzae at 1%(w/v) soluction. Seed germination of radish (Raphanus sativas) and barnyardgrass (Echinochloa crus-galli) was inhibited by 0.5%(w/v) leaf extracts of Pinus densiflora and Quercus acutissima. MeOH extracts of Pinus denislora leaves gave 100% inhibition in seed germintion of radish at 2%(w/v) soluction and showed a complete inhibition in seedling growth of barnyardgrass as well as radish at 5% solution.

• Korean Journal of Food Science and Technology
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• v.46 no.5
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• pp.531-537
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• 2014

Common cruciferous vegetables, kohlabi (Brassica oleracea) and radish (Raphanus sativus), contain phytochemicals such as glucosinolates and carotenoids. Therefore, this study investigated the antioxidant and anti-adipogenic effects of kohlrabi sprout extract (KSE) and radish sprout extract (RSE). The total carotenoid and glucosinolate contents of KSE and RSE were $39.50{\pm}0.67$ and $76.73{\pm}2.75mg/g$, respectively. The total glucosinolate contents of KSE and RSE were $2.65{\pm}0.02$ and $8.13{\pm}0.54mg/g$, respectively. The in vitro-antioxidative activities of KSE and RSE were significantly increased in a dose-dependent manner. Furthermore, ${\beta}$-carotene and glucosinolate-enriched KSE and RSE significantly inhibited lipid accumulation and reactive oxygen species production during the adipogenesis of 3T3-L1 preadipocytes. These results suggest that glucosinolate-enriched KSE and RSE, especially RSE, can be used in the treatment of obesity and as a natural source of antioxidants.