• Title/Summary/Keyword: Raphanus sativus L. var. sativus

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Comparison of Glucosinolate Contents in Leaves and Roots of Radish (Raphanus spp.)

  • Ko, Ho-Cheol;Sung, Jung-Sook;Hur, On-Sook;Baek, Hyung-Jin;Jeon, Young-ah;Luitel, Binod Prasad;Ryu, Kyoung-Yul;Kim, Jung-Bong;Rhee, Ju-Hee
    • Korean Journal of Plant Resources
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    • v.30 no.6
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    • pp.579-589
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    • 2017
  • Glucosinolates (GSLs), beneficial secondary metabolites for human health are abundantly present in radish vegetable. Radish is a member of Brassicaceae family and its seed, leaf and root contain very important GSLs. The objective of this study was to determine the variation of individual and total GSL contents in leaves and roots of 44 radish (Raphanus spp.) germplasm (26 R. sativus L., 3 R. raphanistrum, and 15 R. sativus L. var. raphanistroides Makino), and compare the GSL contents between leaves and roots among three Raphanus species. Thirteen GSLs were identified, being the glucoraphasatin (GRS) and glucobrassicin (GBS) the most abundant aliphatic and indolyl GSLs in both the leaves and roots. Variation in individual and total GSL contents was found among the germplasm of three Raphanus species. The GRS content was higher in roots than that of leaves in all three Raphanus species but the GBS content was higher in leaves than roots. GRS was represented 87.0%, 92.7% and 94.7% of the total GSL in roots of R. sativus L., R. raphanistrum and R. sativus L. var. raphanistroides (Makino) germplasm, respectively. Germplasm of R. raphanistrum exhibited the highest (average, $79.5{\mu}mol/g\;dw$) total GSL with a ranged from 62.7 to $92.9{\mu}mol/g\;dw$. The germplasm IT119288, Joseonmu and IT119262 from R. sativus L., RA 504 and K046542 from R. raphanistrum, and Gyeongju-2003-32 (G2003-32) and IT302373 from R. sativus L. var. raphanistroides (Makino) had high total GSL contents and these could be good candidates for developing the functional compounds-rich varieties in radish breeding program.

Radish(Raphanus sativus L. var. sativus) 추출물의 피부노화 저해효과

  • Kim, Ji-Eun;Hwang, Hyeon-Ik;Lee, In-Sun;Mun, Hye-Yeon
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.863-866
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    • 2001
  • Antioxidative activites and anti skin aging effects of radish(Raphanus sativus L. var. sativus) extracts were studied. The DPPH(1,1-Diphenyl-2-picrylhydrazal) inhibitory effects and CAT(Catalase) activities of hydrophilic radish extracts(DPPH 81.0%, CAT 175units/mg) were significantly higher than lipophilic radish extracts(DPPH 50.0%, CAT 45unitg/mg). But, SOD(superoxide dismutase) activities of hydrophilic radish extracts(SOD 81.3%) were lower than lipophilic radish extracts(SOD 92.3%) In the Bovine Achilles Tendon collagen treated with hydrophilic radish extracts and lipophilic radish extracts, the pyridinoline concentration of collagen were decreased, respectively.

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Genetic Variation in Korean Populations of Wild Radish, Raphanus sativus var.hortensis f. raphanistroides (Brassicaceae)

  • Hur, Man Kyu
    • Journal of Plant Biology
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    • v.38 no.4
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    • pp.329-336
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    • 1995
  • Raphanus sativus L. var. hortensis f. raphanistroides (wild radish: Brassicaceae), a herbaceous perennial, occurs only on beaches in East Asia. Genetic diversity and population structure of seven Korean populations were investigated using starch gel electrophoresis. Although the Korean populatins are small, isolated with patchy distribution, the population maintain a moderate level of genetic diversity; the mean percentage fo polymorphic loci was 51.4%, mean number of alleles per locus was 1.84, and mean expected heterozygosity was 0.116. A combination of animal-outcrossing breeding system, wide geographical distribution, restricted ecological distribution, and a propensity for high fecundity may in part be explanatory factors contributing the moderate level of genetic diversity within populations. An overall excess of homozygotes relative to Hardy-Weinberg expetations (mean FISa=0.116) indicates that consanguineous mating occur within wild radish populations, leading to a family structure within a circumscribed area. Although population of wild radish experience a limited gene flow, only 5% of the total genetic variation found in Korean wild radish populations examined is due to differences among populations (mean GST=0.052). This value is considerably lower than the mean values of species with similar life history and ecological characteristics. However, significant differences were found in allele frequencies between populations for all polymorphic loci (P<0.01). It is supposed that directional selection toward genetic uniformity (similar gene frequencies) in a relatively homogenous habitat is thought to be operated among Korean wild radish populations.

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Selection of Nutrient Solutions and Substrates for Radish (Raphanus sativus L. var. sativus) Growth (20일 무(Raphanus sativus L. var. sativus)의 수경재배에 적합한 양액 및 배지의 선발)

  • Park, K.W.;Hong, H.Y.
    • Journal of Bio-Environment Control
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    • v.5 no.2
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    • pp.236-247
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    • 1996
  • The main objective of this study was to evaluate the effectiveness of nutrient solutions, substrates, and nutrient solution concentrations in substrate culture of radish(Raphanus sativus L. var. sativus). Cooper's, Hoagland & Arnon' 5, and Yamazaki's solution were used to determine the most suitable nutrient solution in deep flow culture(DFC). In result, Yamazaki's solution treatment showed better results than Hoagland's and Cooper's solution treatments in leaf length, leaf number, shoot and root fresh weights. Cooper's solution was much worse than others. Root shape index were low as 0.6 in all treatments. The selection of suitable was conducted among 14 kinds of substrates which were used commercially, such as sand, perlite and peatmoss, in substrates culture. Sand was the most proper in radish growth and shortened the growth periods. Sand also showed better results then others in leaf length, leaf number, shoot and root fresh weight. On the contrary, radish growth in peatmoss was the worst. Generally, root shape index was higher in substrate than in DFC. In order to investigate the suitable ionic strength in radish, Yamazaki's solution was treated with EC of 0.5, 1.0, 1.5, and 2.0 mS/cm. Generally radish growth above 1.0 mS/cm concentration was good, and the best result was shown in 1.5 mS/cm. Vitamin C contents were not significantly different in the roots of radish grown under 1.0 mS/cm or more. The highest vitamin C content was shown in 0.5 mS/cm, and so was thiocyanate content. Anthocyanin contents increased with the increase of the ionic strength in nutrient solution. Mineral nutrient contents had no significant statistical differences between the treatments, but potassium content was remarkably high in 1.5 mS/cm.

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Differential Expression of Three Catalase Genes in the Small Radish (Rhaphanus sativus L. var. sativus)

  • Kwon, Soon Il;Lee, Hyoungseok;An, Chung Sun
    • Molecules and Cells
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    • v.24 no.1
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    • pp.37-44
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    • 2007
  • Three catalase cDNA clones were isolated from the small radish (Raphanus sativus L.). Their nucleotide and deduced amino acid sequences showed the greatest homology to those of Arabidopsis. Genomic Southern blot analysis, using RsCat1 cDNA as a probe, showed that catalases are encoded by small multigene family in the small radish. Nondenaturing polyacrylamide gels revealed the presence of several catalase isozymes, the levels of which varied among the organs examined. The isozyme activities were assigned the individual catalase genes by Northern analysis using total RNA from different organs. The three catalase genes were differentially expressed in response to treatments such as white light, xenobiotics, osmoticum, and UV. Their expression in seedlings was controlled by the circadian clock under a light/dark cycle and/or in constant light. Interestingly, RsCat1 transcripts peaked in the morning, while those of RsCat2 and RsCat3 peaked in the early evening. Our results suggest that the RsCat enzymes are involved in defense against the oxidative stress induced by environmental changes.

A Practical Application on the External Treatment of Acne (A Focus on the Medical Herbs Published in the Woman's Monthly Magazines) (여드름 외용약의 실제적 응용에 관한 고찰(여성 월간지에 여드름 미용도포제로 소개된 본초를 중심으로))

  • Yu Hyun-jung;Choi In-hwa
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.17 no.1
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    • pp.34-44
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    • 2004
  • Objectives: The objective of this study was to teach patients clinically useful external application of acne. Methods: We investigated the effects and manufacturing methods of the medical herbs published in the woman's monthly magazines according to "Zhong-Yao-Da-Ci-Dian(中藥大辭典)". Results: We investigated that Saururus chinensis Baill.(三白草), Houttuynia cordata Thunb.(魚腥草), Coix lachrymajobi L.(薏苡仁) were discovered 3 times, Brassica oleracea L.(甘藍), Raphanus sativus L.(래복), Daucus carota L. var. Sativa DC.(홍래복), Oryza sativa L.(米皮糠), Spinacia oleracea L.(파래), Cucumis sativus L.(黃爪) 2 times, and Glycyrrhiza uralensis Fisch.(甘草), Gallus gallus domesticus Braisson.(?子白), Aloe vera L.(노회), Phascolus radiatus L.(綠豆), Prunus persica Batsch.(桃葉), Lycopersicon esculentum Mill.(番茄), Taraxacum mongolicum Hand.-Mazz.(蒲公英), Prunus armeniaca L.(杏仁), Musa paradisiaca L. var. Sapientum O. ktze.(香蕉) and Malus pumila Mill.(평과) were discovered once in woman's monthly magazine during last two years. According to the effects, we classificated four groups which were defervescence(淸熱), subsidence of edema(消腫), pyorrhea(排膿) and gloss(滋潤) group. Conclusion & discussion : We suggested the use of each medical herbs on the Consensus conference on acne classification. We needed the further study on the manufacturing method, the time of use and the dose

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A study of dietetic therapy on the edema (부종(浮腫)의 식요방법(食療方法)에 관한 연구)

  • Baek, Tae-Hyeun
    • The Korea Journal of Herbology
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    • v.25 no.4
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    • pp.151-160
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    • 2010
  • Objectives : The purpose of this research was to examine dietary therapy on the edema based on the literary findings, for clinical treatment and prevention of the edema. Methods : This Research was examined on histories, compositions, applications, and effects of dietetic treatment about 100 cases of dietary therapy of the edema from Chinese literatures. Results : 1. Various vegetables and animals including herbs, grains, vegetables, fruits, food and drink were used for the dietary therapy. 2. Methods of the preparation for use as therapeutics were decoction, pulvis, gruel, cake, tea, paste, gelatin and etc. 3. Frequently used materials were Cyprinus carpio Linnaeus, Cucumis sativus L. Coix lachrymajobi var. mayuen, Zea mays, Maydis Stigma, Citrullus vulgaris, Zingiber officinale, Cinnamomum cassia Blume, Allium scorodorpasum var. viviparum Regel, Camellia sinensis, Armeniacae amarum Semen, Phyllostachys bambusoides, Luffa cylindrica, Dioscorea batatas Decne, Panax ginseng, Brassica oleracea and Raphanus sativus. Conclusion : Though dietary therapy for the edema is not based on clinical or experimental data, but through experience. It is mostly based on Yin-Yang and five elements, visceral manifestation, channels and their collateral channels and Oriental herbal medicine theories. If we use them properly according to oriental medicine method, it will be effective on treating and preventing the edema.