• Title/Summary/Keyword: Raphanus

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Radish(Raphanus sativus L. var. sativus) 추출물의 피부노화 저해효과

  • Kim, Ji-Eun;Hwang, Hyeon-Ik;Lee, In-Sun;Mun, Hye-Yeon
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.863-866
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    • 2001

  • Antioxidative activites and anti skin aging effects of radish(Raphanus sativus L. var. sativus) extracts were studied. The DPPH(1,1-Diphenyl-2-picrylhydrazal) inhibitory effects and CAT(Catalase) activities of hydrophilic radish extracts(DPPH 81.0%, CAT 175units/mg) were significantly higher than lipophilic radish extracts(DPPH 50.0%, CAT 45unitg/mg). But, SOD(superoxide dismutase) activities of hydrophilic radish extracts(SOD 81.3%) were lower than lipophilic radish extracts(SOD 92.3%) In the Bovine Achilles Tendon collagen treated with hydrophilic radish extracts and lipophilic radish extracts, the pyridinoline concentration of collagen were decreased, respectively.

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The Study on the Solubility of the Ingredients of the Bilestone In the Solution of the Traditional Oriental Medicines (담석 치료에 사용되는 단방요법에 대한 담석성분의 용해 실험)

  • Choi Sung Mo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.5
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    • pp.1387-1390
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    • 2004

  • This study was carried out to investigate the solubility of the ingredients of the bilestone in the solution of the traditional oriental medicines. The cholesterol and the calcium carbonate(CaCO₃) were chosen as the ingredients of the bilestone. Coicis Semen, Polygonum aviculare L, Maydis Stigmata, Allium tuberosum Rottler, and Raphanus sativa var. hortensis for. acanthiformis Makino were studied as the oriental medicines for the bilestone. The cholesterol had showed no solubility in all solutions of the traditional oriental medicines, but the calcium carbonate had showed the good solubility in all solutions of the traditional oriental medicines except Coicis Semen.

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Characteristics of a Low Molecular Weight Minor Anionic Isoperoxidase $A_{3n}$ from Radish

  • Lee, Mi-Young;Kim, Soung-Soo
    • BMB Reports
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    • v.31 no.6
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    • pp.548-553
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    • 1998

  • A minor anionic isoperoxidase named $A_{3n}$, was isolated from Korean radish (Raphanus sativus L.) root. Purification of the enzyme was accomplished by CMcellulose chromatography, DEAE-Sephacel chromatography, and Sephadex G-75 gel filtration. The enzyme was a glycoprotein with molecular weight of approximately 31,000 as determined by SDS-PAGE and 33,000 by Sepadex G-150 gel filtration, which is by far the smallest among the reported isoperoxidases. The pI value was 3.5. The optimum pH of the enzyme was 6.5 for guaiacol and $H_2O_2$, and the $K_m$ values for guaiacol and $H_2O_2$ were 13.3 mM and 1.5 mM, respectively. Kinetic studies with various substrates revealed that only A3n, unlike other isoperoxidases from radish, did not use scopoletin as a substrate and had very low $K_m$ value of 0.25 mM for ferolic acid among naturally occurring phenolic substrates.

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