• Journal of Microbiology and Biotechnology
• /
• 제15권1호
• /
• pp.216-220
• /
• 2005

Periconicin A and B, two new fusicoccane diterpenes originally isolated from the cultures of endophytic fungus Periconia sp. OBW-15, were tested by several biological assays. Periconicin A was consistently more active than periconicin B. In an antifungal activity assay, periconicin A showed potent inhibitory activity against the agents of human mycoses, including Candida albicans, Trichophyton mentagrophytes, and T. rubrum, with minimum inhibitory concentration (MIC) in the range of 3.12- 6.25 $\mug$ /ml. In a plant growth regulatory activity assay, periconicins inhibited hypocotyl elongation and root growth of Brassica campestris L. and Raphanus sativus L. At concentrations below 1 μg/ml, however, both compounds accelerated root growth by 110- 135%. From these results, it is apparent that a methyl group positioned in a cyclopentane ring may play an important role in plant and fungal growth inhibitory activity.

• 한국식품조리과학회지
• /
• 제31권3호
• /
• pp.318-327
• /
• 2015

This study was conducted to investigate the physical, textural and sensory properties of Seolgiddeok prepared with different amount of radish leaf powder, and stored for 3 days. The moisture content decreased gradually during storage and the decrease was less in the groups with radish leaf powder than in the control. The color L value decreased significantly as the amount of radish leaf powder increased, while the redness (a) and yellowness (b) both increased. Measurements with a texture analyzer, showed that the hardness, chewiness, gumminess and adhesiveness of Seolgiddeok tended to decrease in proportion to the amount of radish leaf powder in the formula. The gelatinization properties of Seolgiddeok were investigated by amylograph. Breakdown and setback in Seolgiddeok were low following the addition of radish leaf powder. In the sensory evaluation, Seolgiddeok with radish leaf powder was superior in flavor, chewiness, and softness than the control. Seolgiddeok with 3% radish leaf powder had the highest overall acceptability score. Therefore, we concluded that the addition of radish leaf powder to Seolgiddeok improves the sensory characteristics and delays retrogradation.

• 한국식물병리학회지
• /
• 제14권6호
• /
• pp.625-629
• /
• 1998

Turnip mosaic potyviruses (TuMV) were isolated from Rorippa indica and Armoracia lapathifolia showing mosaic symptoms in field. Identification of the TuMVs were carried out by host reactions of indicator plants, electron micrograph, serological properties and reverse transcription-poly-merase chain reaction (RT-PCR). Both viruses systemically infected Chenopodium quinoa, Nicotiana clevelandii, Brassica rapa, B. campestris subsp. pekinensis, B. juncea and Raphanus sativus, and developed local infection on inoculated leaves of C. quinoa, C. amaranticola, C. album, N. tabacum cv. Xanthi nc and Gomphrena grobosa. However, the viruses did not infect on N. glutinosa, Cucumis sativus and Vigna unguiculata. The filamentous particles, about 720 nm in length, and inclusion bodies were observed from the infected leaf tissues by dipping on electron microscopy. Crude sap of leaf infected with the viruses was reacted positively with an antiserum of TuMV in agar gel double diffusion. For detection of the viruses, RT-PCR was carried out with TuMV--specfic oligonucleotide primer. The RT-PCR products, a 1,092 bp DNA fragment, were obtained from naturally infected leaves of R. indica and A. lapathifolia. In inoculation test to seven cruciferous weeds with TuMV, infection occurred in Arabis glabra, Barbarea orthoceras, Capsella bursa-pastoris, Draba nomorosa var. hebecarpa, Rorippa cantoniensis and Thlaspi arvense.

• International journal of advanced smart convergence
• /
• 제7권3호
• /
• pp.146-154
• /
• 2018

In this study we investigated antioxidant and anti-inflammatory activities of Malus Domestica (apple), Pyrus Communis L. (pear), Daucus carota L. (carrot), Brassica oleracea var. (broccoli), Brassica oleracea var. capitata (cabbage) and Raphanus sativus L. (radish), that were obtained from local market. As these are common fruits and vegetables that are widely consumed, we aimed to investigate their beneficial properties especially the antioxidant and anti-inflammatory. The samples were processed by an indirect heating method and their properties were compared to their raw forms. Based on DPPH and ABTS assay, processed samples showed better antioxidant activity compared to raw samples, and processed pear sample had the best antioxidant activity. The anti-inflammatory activities of the samples were also investigated in LPS-treated RAW 264.7 cells. The mRNA expressions of pro-inflammatory mediators and cytokines (iNOS, COX-2, $TNF-{\alpha}$, $IL-1{\beta}$ and IL-6) were assessed by RT-PCR. Processed samples exhibited better inhibition of iNOS, compared to the raw forms. Processed broccoli and cabbage samples exhibited outstanding anti-inflammatory effects. The samples did not exhibit cytotoxicity against RAW 264.7 cells up to 1mg/ mL as shown in the cell viability assay. Taken together, processed broccoli and cabbage samples exhibited the strongest anti-inflammatory properties.

• 한국토양비료학회지
• /
• 제31권1호
• /
• pp.56-60
• /
• 1998

크롬에 의한 무와 배추 유식물의 생리학적 반응을 조사하기 위하여 수경재배 조건하에서 크롬용액의 농도에 따른 무와 배추종자의 발아율 세포신장, ${\alpha}$-amylase활성도, 엽록소, 단백질함량의 차이를 비교하였다. 크롬처리농도가 증가함에 따라 농도의존적으로 무와 배추종자의 발아율, 세포신장, ${\alpha}$-amylase 활성도가 감소하였는데 배추에서 보다 무에서 그 저해정도가 심하게 나타났다. 무와 배추에서 크롬처리시 약간의 chlorophyll 함량 감소가 있었으며 chlorophyll a가 b보다 더 저해를 받았다. 크롬농도 증가에 따른 ${\alpha}$-amylase 활성도, chlorophyll a와 b, 단백질함량과의 관계를 다중회귀분석한 결과 단백질> ${\alpha}$-amylase>chlorophyll a>chlorophyll b의 순서로 저해를 받았다.

• 식물병연구
• /
• 제29권1호
• /
• pp.64-71
• /
• 2023

In October 2021, soft rot disease seriously affected radish crop in Dangjin, Chungcheongnam-do, Korea. The infected radishes were stunted and turned dark green, with yellowish leaf foliage. A slimy, wet, and decayed pith region was observed in the infected roots. The bacterial strain KNUB-03-21 was isolated from infected roots. The biochemical and morphological characteristics of the isolate were similar to those of Pectobacterium brasiliense. Phylogenetic analysis based on the sequences of the 16S rRNA region and the concatenated DNA polymerase III subunit tau (dnaX), leucine-tRNA ligase (leuS), and recombinase subunit A (recA) genes confirmed that the isolate is a novel strain of P. brasiliense. Artificial inoculation of radish with P. brasiliense KNUB-03-21 resulted in soft rot symptoms similar to those observed in infected radish in the field; subsequently, P. brasiliense KNUB-03-21 was reisolated and reidentified. To our knowledge, this is the first report of P. brasiliense as a causal pathogen of radish soft rot in Korea.

• Preventive Nutrition and Food Science
• /
• 제6권3호
• /
• pp.192-196
• /
• 2001

Ethanol extracts from 36 vegetable samples were assayed for their growth-promoting effects on Bifidobacterium bifidum, B. longum, and Lactobacillus casei The growth-promoting effects varied according to bacterial strain and vegetable species. In modified Gy rgy broth, extracts of Lactuca sativa, Lycopersicon esculentum and L escutentum var. cerasiforme exhibited strong growth-promoting responses toward B. longum, and significant and strong growth- promoting response toward B. bifidum was observed in extracts of Actinidia arguta, Allium cepa, A. sativum, Brassica campestris subsp. napus vats. pekinensis, Capsicum frutescens, Daucus carota var. sativa, L sativa, 1. esculentum and L. esculentum var. cerasforme, Nelumbo nucifera, Cucurbita moschata, Lackca sativa var. capitata, and Rubus coreanus. For L casei, extracts of A. fshlosum, A. hberosum, Cichorium intbus, Cucurbita moschat\ulcorner Ipomoea batatas, 1. sativa var. capitata, L. esculentum, P. brachycarpa, Raphanus sativus, R. coreanus, and S. melongena strongly enhanced the growth of this bacteria. In modified Gy rgy broth, the promoting effect was most pronounced with B. bifidum and L. casei among lactic acid bacteria used. In MRS broth, A. arguta, A. cepa, A. sativum, B. campestris subsp. napus var. pekinensis, C. frutescens, and D. carota var. sativa L. satiw var. capitata, and R. coreanus strongly enhanced the growth of B. bifidum, Growth of B. longum was strongly affected by the addition of extracts from L. sativa var. capitata. For L casei, moderate growth-promoting responses were observed in 9 vegetable extracts. The promoting effect in MRS broth was most pronounced with B. bifidum among lactic acid bacteria used.

• 한국식품조리과학회지
• /
• 제15권1호
• /
• pp.61-67
• /
• 1999

The effects of salting methods on Kakdugi (cubed radish kimchi) fermentation were evaluated. Kakdugi was prepared with various salting methods, salt concentrations, and settling times, and fermented at 10$^{\circ}C$ for up to 52 days. Radish (Raphanus sativus L.) cubes (2 cm size) were salted by using the following methods salt concentration of about 1.5% which was known appropriate for the organoleptic quality of Kakdugi: 1) Treatment S-1: applying dry salt uniformly onto the radish cubes, with a salt concentration of about 1.5% (w/w) and cured for 1 hr, 2) Treatment S-5: applying dry salt uniformly onto the radish cubes, with a salt concentration of about 1.2% (w/w) and cured for 5 hr, 3) Treatment B-1: brining radish cubes in a 8.5% (w/v) salt solution for 1 hr, 4) Treatment B-5: brining radish cubes in a 4.0% (w/v) salt solution for 5 hr. As the fermentation continued, the initial high decrease in pH has been retarded in all the treatments, of which the delaying extent was more significantly noted from B-1 and B-5 than S-1 and S-5. The pH of the Kakdugi which showed a good eating quality dropped to 4.3∼4.8 with the accumulation of total acids. Total vitamin C increased sharply at the palatable period of Kakdugi during the initial fermentation and then decreased gradually following a sigmoidal changing pattern. The reducing sugar levels were also influenced by salting methods and fermentation as sugars are converted into acids. High initial contents of reducing sugars and their subsequent rapid decrease were observed in “S” group than “B” group during fermentation. For nonvolatile organic acids, lactic acid increased consistently throughout the fermentation while malic acid, which was high at the initiation of fermentation, decreased rapidly afterwards at the palatable period of Kakdugi.

• 식물병연구
• /
• 제16권3호
• /
• pp.232-237
• /
• 2010

Tomato spotted wilt virus(TSWV)의 상습 발생지인 안양지역에서 가지, 알타리무, 슈가로프에서 TSWV가 발생하였다. 알타리무에서는 잎에 괴저반점과 뿌리에 괴저 병징이 나타났다. 가지에서는 잎에 전형적인 다중 원형반점을 나타냈으며 열매에 심한 괴저를 나타냈다. 슈가로프에서는 잎에 전형적인 원형반점과 심한 위축 병징이 나타났다. 가지, 알타리 무, 슈가로프에서 분리한 TSWV의 생물적 특성은 흰 명아주, 붉은 명아주, 담배(N. devney)에서는 국부 감염이었으며, 담배(N. glutinosa, N. benthamiana)와 독말풀(D. stramonium)에서는 전심감염이었다. 가지와 슈가로프에서 분리한 TSWV는 병원성이 유사하였으나, 알타리 무에서 분리한 TSWV는 N. tabacum 'Xanthi NC' 등 5종의 담배에서 국부 감염되어 병원성이 매우 상이하였다.

• 환경생물
• /
• 제19권3호
• /
• pp.205-210
• /
• 2001

묵은 배추와 무 종자에 저선량 ${\gamma}$선을 조사하여 종자발아율과 발아유식물의 효소활성 변화를 조사하였다. 발아율은 대조구에 비해 저선량 조사구에서 증가하는 경향을 보였는데 특히 발아초기단계에 효과가 뚜렷하게 나타났으며 배추는 2 Gy와 8 Gy조사구에서 4-ll % 정도, 무는 2 Gy, 6 Gy, 10 Gy 조사구에서 25-41% 정도 증가하였다. 저선량 ${\gamma}$선이 조사된 배추와 무의 유묘초장도 저선량 조사구에서 증가하였는데 특히 배추의 경우는 4 Gy와 l0 Gy 조사구에서, 무는 6 Gy 조사구에서 뚜렷한 증가효과를 보였다. 저선량 조사구의 단백질 함량은 발아초기단계에 대조구에 비해 증가하였으며 POD와 CAT 활성은 4 Gy와 l0 Gy 조사구에서 증가하였다. 이에 저선량 $\gamma$선에 의해 묵은 종자의 발아와 효소활성이 촉진됨을 확인할 수 있었다.