• BMB Reports
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• v.52 no.9
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• pp.572-576
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• 2019

Bisphosphonates are the mainstay of therapy worldwide for osteoporosis. However, bisphosphonates also have limitations. The objective of this study was to determine the role of miR-101-3p/Rap1b signal pathway in osteoclast differentiation after treatment with bisphosphonates. Our results revealed that miR-101-3p was an important regulator in bisphosphonates treated-osteoclasts. When miR-101-3p was down-regulated in bone marrow-derived macrophage-like cells (BMMs), the development of mature osteoclasts was promoted, and vice versa. However, alendronate decreased multinucleated cell number regardless of whether miR-101-3p was knocked down or over-expressed. TRAP activity assay confirmed the above results. Luciferase assay indicated that miR-101-3p was a negative regulator of Rap1b. Western blot analysis revealed that protein expression level of Rap1b in BMMs transfected with OV-miR-101-3p was lower than that in BMMs transfected with an empty vector. Rap1b overexpression increased TRAP-positive multinucleated cells, while Rap1b inhibition decreased the cell numbers. In vivo data showed that miR-101-3p inhibited osteoclast differentiation in ovariectomized mice while overexpressed of Rap1b blocked the differentiation. Taken together, our data demonstrate that miR-101-3p/Rap1b signal pathway plays a key role in osteoclast differentiation after treatment with bisphosphonates.

• Korean Journal of Veterinary Service
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• v.27 no.1
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• pp.95-101
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• 2004

Babesia bovis rap-1 and B equi ema-1 intergenic(IG) nucleotides were analyzed and compared for identifying putative promoter sites using computer programs. The reason to initiate this research was to determine if IG nucleotides of Babesia genes that are predicted to be involved in erythrocyte invasion have functions regulating gene transcription and translation, which can be applied to functional gene knockout. Four IG sequences used included BbIG5(B bovis rap-1 5' IG), BblG3(B bovis rap-1 3' IG), BeIG5(B equi ema-1 5' IG) and BeIG3(B equi ema-1 3' IG). BbIG5 contained a putative promoter at nucleotide 197-246 with a predicted TATA-box and a transcription start site. BbIG3 had a putative promoter at nucleotide 270-320 with two predicted TATA-boxes and a transcription start site. BeIG3 had a putative promoter at nucleotide 155-205 with a predicted TATA-box and a transcription start site. Putative promoter sites in these three sequences mentioned above were identified with score cutoff 0.8, which means detection of about 40% recognized promoters with 0.1-0.4% false positives. In contrast, BeIG5 had a putative promoter at nucleotide 163-213 with score cutoff 0.8, but neither TATA-box nor transcription start site were recognized. However, BeIG5 had a putative promoter at nucleotide 388-438 with a predicted TATA-box and a transcription start site when score cutoff was decreased to 0.18, which means detection of about 70% recognized promoters with 2.2-5.3% false positives. These sequences with putative promoters can be tested if they have functions regulating gene transcription and translation.

• International Journal of Highway Engineering
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• v.17 no.2
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• pp.21-30
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• 2015

PURPOSES : The purpose of this study was to evaluate the performance of rapid-setting polymer-modified asphalt mixtures with a high reclaimed asphalt pavement (RAP) content. METHODS: A literature review revealed that emulsified asphalt is actively used for cold-recycled pavement. First, two types of rapid-setting polymer-modified asphalt emulsion were prepared for application to high-RAP material with no virgin material content. The quick-setting polymer-modified asphalt mixtures using two types of rapid-setting polymer-modified asphalt emulsion were subjected to the following tests: 1) Marshall stability test, 2) water immersion stability test and 3) indirect tensile strength ratio test. RESULTS AND CONCLUSIONS : Additional re-calibration of the RAP was needed for laboratory verification because the results of analyzing RAP aggregates, which were collected from different job sites, did not deviate from the normal range. The Marshall stability of each type of binder under dry conditions was good. However, the Type B mixtures with bio-additives performed better in the water immersion stability test. Moreover, the overall results of the indirect tensile strength test of RAP mixtures with Type B emulsions exceeded 0.7. Further research, consisting of lab testing and on-site application, will be performed to verify the possibility of using RAP for minimizing the closing of roadways.

• IMMUNE NETWORK
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• v.9 no.2
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• pp.35-40
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• 2009

Although Rap GTPases of the Ras family remained enigmatic for years, extensive studies in this decade have revealed diverse functions of Rap signaling in the control of cell proliferation, differentiation, survival, adhesion, and movement. With the use of gene-engineered mice, we have uncovered essential roles of endogenous Rap signaling in normal lymphocyte development of both T- and B-lineage cells. Deregulation of Rap signaling, on the other hand, results in the development of characteristic leukemia in manners highly dependent on the contexts of cell lineages. These results highlight crucial roles of Rap signaling in the physiology and pathology of lymphocyte development.

• International Journal of Highway Engineering
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• v.18 no.2
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• pp.61-71
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• 2016

PURPOSES: The objective of this study was to determine the optimum ratio of mix design, for a reclaimed asphalt pavement (RAP) content of 100%, for spray injection application. METHODS: A literature review revealed that spray injection is an efficient and cost-effective application for fixing small defective regions of an asphalt pavement. Rapid-setting polymer-modified asphalt mixtures prepared from two types of rapid-setting polymer asphalt emulsion were subjected to Marshall stability and wet track abrasion tests, in order to identify the optimum mix designs. RESULTS and CONCLUSIONS : Different mix designs of type A and type B emulsions were prepared using RAP and virgin aggregates, in order to compare the performance and determine the optimum mix design. The performance of mixtures prepared with RAP was superior to that of mixtures containing virgin aggregates. Moreover, for optimum ratio of the design, the binder content prepared from RAP was set to 1~2% lower than that consisting of virgin aggregates. Compared to their Type A counterparts, type B mixtures consisting of a reactive emulsion performed better in the Marshall stability and wet track abrasion tests. The initial results confirmed the advantages associated with using RAP for spray injection applications. Further studies will be performed to verify the difference in the optimum mix design and performance obtained in the lab-scale test and tests conducted at the job site by using the spray injection machine.

• International Journal of Highway Engineering
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• v.19 no.2
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• pp.75-85
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• 2017

PURPOSES : The purpose of this study was to determine the optimum mix design of the content of 100 % reclaimed asphalt pavement (RAP) for spray injection application with different binder types. METHODS : Literature review revealed that spray injection method is the one of the efficient and economical methods for repairing a small defective area on an asphalt pavement. The Rapid-Setting Polymer modified asphalt mixtures using two types of rapid setting polymers-asphalt emulsion and a quick setting polymer asphalt emulsion-were subjected to the following tests to determine optimum mix designs and for performance comparison: 1) Marshall stability test, 2) Retained stability test, 3) Wet track abrasion test, and 4) Dynamic stability test. RESULTS and CONCLUSIONS : Type A, B, and C emulsions were tested with different mix designs using RAP aggregates, to compare the performances and determine the optimum mix design. Performance of mixtures with Type A emulsion exceeded that of mixtures with Type B and C emulsion in all aspects. In particular, Type A binder demonstrated the highest performance for WTAT at low temperature. It demonstrated the practicality of using Type A mixture during the cold season. Furthers studies are to be performed to verify the optimum mix design for machine application. Differences in optimum mix designs for machine application and lab application will be corrected through field tests.

• Journal of Embryo Transfer
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• v.31 no.4
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• pp.323-333
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• 2016

Pro-inflammatory cytokines, interleukin-$1{\beta}$(IL1B), IL6, and tumor necrosis factor-alpha (TNF), are known to play important roles in regulating the endometrial function in the uterus during the estrous cycle and pregnancy in several species. However, the expression and function of these cytokines and their receptors in the uterine endometrium during the estrous cycle have not been studied in pigs. Thus, this study determined the expression and regulation of IL1B, IL6, TNF and their respective receptors, IL1R1, IL1RAP, IL6R, GP130, TNFRSF1A, and TNFRSF1B during the estrous cycle in pigs. To analyze levels of each gene expression in the uterine endometrium we obtained from endometrial tissues on Days 0, 3, 6, 9, 12, 15, and 18 of the estrous cycle. Real-time RT-PCR analysis showed that levels of IL1B, IL1RAP, IL6R, GP130, TNF, TNFRSF1A, and TNFRSF1B mRNAs were highest on Day 15 or 18 of the estrous cycle, which corresponds to the proestrus period. Levels of IL1R1 were highest on Day 0, while levels of IL6 were biphasic with high levels on Day 6 and Day 15. The abundance of IL1B, IL6, IL6R, and TNF mRNAs was decreased by progesterone, while levels of GP130 were increased by progesterone in endometrial tissue explants. These results showed that expression of pro-inflammatory cytokines and their receptors changed stage-specifically during the estrous cycle and regulated by progesterone in the uterine endometrium in pigs, suggesting that these pro-inflammatory cytokines may be involved in the regulation endometrial function during the estrous cycle in pigs.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.34-34
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• 2023

화본과 식물인 홍띠(Imperata cylindrica 'Rubra') 식물체의 기관분화 단계별 시료를 작성하여 이들 몇가지 기관분화에 관련된 유전자의 각 단계별 기관분화체에서 유전자존재와 발현여부를 조사하였다. 또한 식물의 기관분화에 관련된 유전자 정보를 얻기 위하여 일부 유전자의 특성을 정리하였다. 조사된 기관분화 관련 유전자중 탈분화 관련 유전자로는 FIF, RAP2-4 (WIND1) 유전자 등이, shoot 분화 관련 유전자로는 WUS, 부정근 분화관련 유전자로는 OsSCR, WOX11 등, 체세포배발생 관련 유전자로는 BBM1, SERK1, LEC1B, MEA 유전자 등이다. 이들 유전자중 RAP2-4(WID1), FIE, BBM1, SERK1, OsSCR, WOX11, WUS, LEC1B 유전자 등 8종의 기관분화 관련 유전자를 대상으로 화본과 식물의 기관분화의 각 단계별 기관분화체를 작성하여 PCR을 통하여 유전자(gDNA)의 존재여부를 확인한 결과 공시 유전자 모두 홍띠의 각단계 기관분화체에서 존재하였다. 또한 상기 유전자를 사용하여 화본과 식물의 각단계별 기관분화체에 대하여 유전자 발현을 확인한 결과 각단계 기관분화체에서 모두 발현하였다. 5종류 총 15개체의 기관분화 단계별 분화체에서 캘러스 발생 유전자인 FIE는 모식물체 1번을 제외한 14개의 식물체에서 모두 관찰되었으며, 뿌리 발생 유전자인 WOX11은 15개의 모든 식물체에서 탐색되었으며, 체세포 발생 유전자인 LEC1B는 15개 식물체에서 모두 발현하였으나 비교적 약하게 발현하였다. 이상과 같이 본 연구에서는 식물 기내발생시 기관분화관련 유전자의 동향을 파악하여 식물발생학의 기초자료를 구축하고자 하였다.

• Journal of the Korean Society of Costume
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• v.59 no.2
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• pp.154-168
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• 2009

This study aims on providing a design technique that expresses aesthetical elements by arranging the analysis of sensual beauty into detailed elements of design from music video outfits by genre of pop music, by observing music videos of female vocalists chosen from each genre of pop music focusing on their fashion. The results of this study are the following. 1. In the genre rock, the sensual beauty of female were expressed with a boyish and neutral style using texture such as leather or denim, and such style had the effect of emphasizing their feminine side even more. 2. In the genre dance music, exposure is extensive compared to other genre using sexy or lingerie look, and I found an ambivalent style of feminism with clothes in the form of drapery using textures such as chiffon and silk, and femme fatale style with textures adhering to the body such as leggings, leotard, and bodysuit. 3. In the genre of rap and hip-hop, clothes from casual and costume-play style were found using training jersey, t-shirt, and denim pants, and emphasized the sensual beauty of women by showing a silhouette with short length and fitting style using shiny textures. 4. In the genre of R&B, there were diverse outfits that suits the characteristics of characters appearing in the stories, or the situation of the story since there are many dramatic representation in the form of story Especially in case of female characters, the feminine side was emphasized staging a feminine style by wearing dresses with the texture of chiffon and silk. Exposure was restrained compared to other genre.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1994.10a
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• pp.689-694
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• 1994

In this paper, to develop a smart CAD/CAM system for systematically performing from the 3-D solid shape design of products to the CNC cutting operation of products by a machining center, a B-Rep solid modeler is realized based on the half edge data structure. Because the B-Rep solid modeler has the various capabilities related to the solid definition functions such as the creation operation of primitives and the translational and rotational sweep operation, the solid manipulation functions such as the split operation and the Boolean set operation, and the solid inversion function for effectively using the data structure, the 3-D solid shape of products can be easily designed and constructed. Also, besides the automatic generation of CNC code, the B-Rep solid modeler can be used as a powerful tool for realizing the automatic generation of finite elements, the interference check between solids, the structural design of machine tools and robots and so on.