• Food Science and Biotechnology
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• 제15권4호
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• pp.599-602
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• 2006

The effects of ethanol extract from radish seeds on gastrointestinal function in rats were investigated. Rats were divided into 2 groups of 8 rats each, the basal group and the radish seed ethanol extract group. The radish seed ethanol extract group had a significantly decreased gastrointestinal transit time, but a significantly increased fecal weight compared with the basal group. The radish seed ethanol extract group also had significantly higher duodenal villus height and greater colonic mucosal thickness than the basal group. The radish seed ethanol extract group had a greater proliferation of 5-bromo-2-deoxy-uridine (BrdU) immunoreactive cells in the gastric mucosa as well as in the mucosa and submucosa of the small and large intestine than did the basal group. Thus, radish seed ethanol extract may be useful in preventing constipation based on the observation of an increase in fecal weight, a decrease in gastrointestinal transit time, and positive changes in the intestinal mucosa.

• 한국작물학회지
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• 제54권4호
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• pp.346-350
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• 2009

The cultivated radish (Raphanus sativus L.) is a major vegetable crop in the world wide and fast-growing species that grows inhabitats of six continents. It is very important to determine hybrid seed purity in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in $F_2$-hybrid radish cultivars demonstrated. One hundred eighty seeds from the F1 male and female harvest were subsequently screened for seed purity using 13 primers. The 13 primers result in 17 cultivar-specific bands and 23 variable RAPD bands scored for cultivar. RAPD analysis of hybrid seeds from the harvest revealed 128 seeds tested except underdevelopment and decayed seeds were sibs. Especially, $F_2$ hybrids of radish, OPC13, OPD20 were presented clear hybrid bands. It maintains higher than average level of genetic diversity compared with their correspondent parents. RAPD amplification of DNA extracted from germinated individuals from the female harvest reveal that 10 of 208 seeds tested were self-inbred (4.8%). RAPD analysis of hybrid seeds from the male harvest revealed 7 of the 208 seeds tested were sibs (3.4%). The RAPD may lead to a better insight in to the hybrid seed purity.

• Food Science and Biotechnology
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• 제18권2호
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• pp.390-395
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• 2009

Red radish seeds were irradiated at doses up to 8 kGy using electron beam (e-beam) and gamma ray ($\gamma$-ray). The seed viability and functional properties (carotenoid, chlorophyll, ascorbic acid, and total phenol) of sprouts grown from these irradiated seeds were evaluated. High germination percentage ($\geq$97%) was observed in seeds irradiated at $\leq$5 kGy, but the yield ratio and sprout length significantly decreased with increased irradiation dose. Irradiation at $\geq$6 kGy resulted in curling of the sprout roots. Sprouting enhanced the functional properties of red radish seeds as indicated by the increased carotenoid, chlorophyll, ascorbic acid, and total phenol contents during germination. However, radiation treatment hampered the growth of seeds resulting in underdeveloped sprouts with decreased carotenoid, chlorophyll, ascorbic acid, and total phenol contents. In general, e-beam and $\gamma$-ray irradiation of red radish seeds showed similar effects on the seed viability and functional properties of sprouts. Postharvest storage reduced the functional quality of sprouts.

• 한국식품저장유통학회지
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• 제15권5호
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• pp.754-759
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• 2008

새싹 채소 중 무순의 위생적인 재배 조건을 설정하기 위하여 종자세척 및 관수로서 이산화염소수, citric acid 및 glycerin의 적용 효과를 조사하였다. 무 종자에 인위적으로 오염시키기 위하여 Salmonella typhimurium은 실험실 조건에서 배양하였으며, 무 종자를 S. typhimurium 현탁액에 침지시켜 균수를 약 $10^6\;log\;CFU/g$로 조절하였다. 무 종자에 대한 세척 효과는 이산화염소수 및 citric acid의 단독처리보다 병행처리시 더욱 우수한 효과를 나타내어, 이산화염 소수 200 ppm과 citric acid 2%의 병행처리시 2.89 log CFU/g 의 감소효과를 나타내었다. 또한, glycerol 0.5%/citric acid 2.0% 처리의 경우 3.46 log CFU/g, glycerol 0.5%/이산화염 소수 200 ppm 처리는 2.14 log CFU/g의 S. typhimurium 균의 감소를 나타내어, glycerol 단독 처리보다 병행처리구가 더 우수한 효과를 나타내었다. 무 종자의 발아 및 성정에 따른 균수 감소는 비록 종자에 대한 처리효과가 유의적으로 있었음에도 불구하고 새싹 성장 후 균수의 차이는 미미하였다.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1630-1630
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• 2001

NIR specroscopy is widely used today as a quantitative technique for predicting the chemical composition of various agricultural product. However there exist few application for seed quality assessment, especially for seed germinability. This study is to show the possibilities of a nondestructive estimation of germinability in radish (Raphanus sativus L) seeds. The experiment carried out on one radish cultivar (Chung Su Gung Jung, Nong Woo Bio Co., Ltd.) harvested in 1993. NIR(Foss Co.) spectral measurements were carried out on the seeds surface of flat side. The seeds after spectral measurements were planted on blotter individually and observed germination. The seeds were characterized to nongermination and germination group, which in turn grouped to normal and abnormal germination and then compared with the NIR spectra. The spectra from these seed groups were compared each other, The result suggested that NIR spectra could be applicable to determine radish seeds germinability.

• 한국식생활문화학회지
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• 제35권6호
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• pp.577-581
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• 2020

This study examined the physicochemical properties of radish pickle containing different natural preservatives (grapefruit seed extract, green tea extract, rosemary, or olive) stored for 0, 1, 2, 3, and 4 weeks. The hardness and color of the radish pickles with the grapefruit seed extract was higher than the other radish pickles during storage from week 0 to week 4. A 14.52% and 13.80% decrease in hardness and color were observed in the radish pickles with grapefruit seed extract (GFE), respectively. In addition, the total phenolic content was highest in the GFE in natural preservatives. Based on the results, GFE was selected as the optimal natural preservatives, and the growth of total viable bacteria and yeast were evaluated. The total viable bacteria and yeast showed similar patterns to the control. These results are expected to be useful in producing radish pickles with optimal quality and contribute to the development of various foods in the food industry.

• 생명과학회지
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• 제18권7호
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• pp.907-911
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• 2008

무(Raphanus sativus L.)는 세계적으로 중요한 작물 중의 하나이다. 십자화과 식물 종자 생산에서 원하지 않은 내교잡에 의한 종자 결실로 오염이 발생하므로 씨의 순수성 검증은 매우 중요하다. 재배종 진주 대평 무(R. sativus cv. Daepeng)와 백자 무(R. sativus cv. Backza)의 교잡 분석을 실시하였다. 알로자임으로 상업적으로 이용되는 잡종 제1세대($F_1$) 무에 있어서 씨의 순수성을 평가하였다. 웅성과 자성 양친 360개체에 27개 대립유전자좌위를 조사하였다. 특히 Par-1 ($aa{\times}bb$), Lap-1($aa{\times}bb$), Est-1 ($aa{\times}bb$)에서 명확한 잡종 밴드를 나타내었다. Est-1 대립유전자좌위에서 자성 배우체로부터 기원된 것이 15개체(8.3%)가 발견되었고, 웅성 배우체로부터 기원된 것이 26개체(14.4%)가 발견되었다. 또한 다양도 측면에서 양친 계통에 비해 잡종 계통에서 높은 유전적 다양도를 유지하고 있었다. 샤논의 정보지수(Shannon's index)를 이용한 표현형 다양도는 교잡 계통이 가장 높았다. 알로자임에 의한 무 계통의 교잡에 의한 종자 생성에서 씨에 대한 순수성 검증이 효과적으로 탐지되어 육종 연구에 기여할 수 있을 것으로 판단된다.

• 동아시아식생활학회지
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• 제9권1호
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• pp.74-80
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• 1999

The proximate composition, pH, vitamins and minerals in sunflower seed sprout were investigated to furnish basic data for utilization in health food or processed food. The pH of sunflower seed sprout was 5.70. The contents of moisture, crude protein, crude fat, crude ash and crude fiber of sunflower seed sprout were 94.7%, 1.3%, 0.3%, 1.3% and 1.6%, respectively. The vitamin A, vitamin B$_1$, vitamin B$_2$, vitamin C and niacin contents in sunflower seed sprout were 114.411. U%, 0.06mg%, 0.05mg%. 5.90mg% and 0.80mg%, respectively. The contents of Ca, P, Fe, Na, K, Mn, Cu, Zn and Mg in sunflower seed sprout per 100g were 80.00mg, 4.85mg, 3.63mg, 8.25mg, 180.90mg, 1.35mg, 0.43mg, 1.85mgand 66.35mg, respectively. The crude ash and crude fiber content of sunflower seed sprout were 3 or 4 times higher than those in the sprout of radish seed, mung bean, soybean or alfalfa, respectively.

• Food Science and Biotechnology
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• 제17권4호
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• pp.878-882
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• 2008

The efficacy of citric acid-aqueous chlorine dioxide ($ClO_2$) treatment of radish seeds artificially contaminated with Escherichia coli was studied. Radish seeds were inoculated with E. coli. Following inoculation, samples were stored at $4^{\circ}C$ and soaked in citric acid or aqueous $ClO_2$ for 10 min. The treatment of radish seeds using 200 ppm aqueous $ClO_2$ solution caused a 1.5 log CFU/g reduction in the population of E. coli. Compared to the aqueous $ClO_2$ treatment, soaking radish seeds in 2.0% citric acid solution for 10 min was more effective in reducing E. coli populations on radish seeds. The efficacy of spray application of chlorine (100 ppm) or 0.5% citric acid to eliminate E. coli during the germination and growth of radish was investigated. Radish seed inoculated with E. coli was treated for the duration of the growth period. Although it resulted in a decrease in the E. coli population, the spray application of 100 ppm chlorine during the growth period was not significantly effective. In contrast, the combined treatment of seeds using 200 ppm aqueous $ClO_2$ and treatment of sprouts with 0.5% citric acid solution during sprout growth was hardly effective in eliminating E. coli.

• Journal of Biosystems Engineering
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• 제25권6호
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• pp.463-470
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• 2000

For maximum seeding efficiency of a nozzle type seeder, the performance of the nozzle should be considered sufficiently. This study was carried out to investigate the optimum operating conditions of a seeder attached the vacuum nozzle which was modified syringe needle acting on the plug seedling tray and the seed plate. Such operating factors as the hole diameter of the nozzle (d), the distance from the nozzle tip to the bottom plate of seed hopper(D) the absorbing air pressure of the nozzle tip(P) the bounding height of seed from the vibrated bottom plate of seed hopper and the seeding speed were selected based on the weight of a grain of seed(W). The treated materials were pepper seed as the flat type, cucumber seed as the oval type and radish seed as the spherical type. The optimum operating conditions of the experimental seeder were revealed as follows: 1. The height of the seed bounding from the bottom plate of seed hopper and the distance from nozzle tip to bounded seed were 5 mm and 0.5 mm at all seeds. The hole diameter of the nozzle and the absorbing pressure for pepper seed, cucumber seed and radish seed was 0.45 mm, 0.65 mm. 0.65mm and 39.2 kPa, 88.3 kPa, 58.8 kPa, respectively. 2. The absorbing pressure P was represented as P=η.4W/$\pi$d$^2$ where η was 100. The seeding speed using a 128 cell tray was 2.4 cm/s which was same transfer as 2.5 trays per minute. 3. The maximum seeding rate in case of the pepper seed was 97% the cucumber seed was 95% and the radish seed was 100% under the optimum operating conditions of the seeder.