This study was carried out to investigate relationships between maximal running time (MRT) and glycogen supercompensation in fast twitch white fibers (white vastus, WV), fast twitch red fibers (red vastus, RV) and slow twitch red fibers (soleus muscle, SM) of endurance-trained rats. Male rats of a Sprague-Dawley strain were divided into the trained groups and untrained groups. Untrained groups were acquired to run on the treadmill 10 minutes for 3 days and remained rest and maintained with mixed diet for 4 weeks. For last 10 days of resting period, the untrained rats were divided into 3 groups i.e. mixed diet (untrained control), high and low carbohydrate (CHO) diet groups. And each group was subdivided into 2 groups, one group was tested for the MRT and the other was sacrificed to measure the blood glucose, blood lactate, glycogen contents of liver and muscles. The experimental groups were trained on treadmill by a modified method of Constable et al. (1984) maintained with mixed diet for 4 weeks. After measurement of MRT of this group, they were also divided into high and low CHO groups and fed with these diet for 2 days and MRT of each group was measured again to see the effect of high or low CHO feeding on the MRT. Each group was maintained with the same diet for next 2 days during which some of the rats were sacrificed at given time intervals for the measurements of blood glucose and lactate, liver and the muscles glycogen. The results were summarized as follows; 1) In the untrained group, there were no significant differences between subgroups in MRT, glycogen conent of SM, RV and WV. But blood glucose concentration and glycogen content of liver of low CHO group were significantly lower than those of mixed diet group. 2) The MRT and glycogen content of SM, RV and WV of trained mixed diet group were significantly increased compared to those of untrained mixed diet group, but there was no significant difference in glycogen content of liver. 3) MRT of trained mixed, high CHO and low CHO groups were $137{\pm}9.8,\;176{\pm}9.8\;and\;129{\pm}7.3\;min$ respectively with the significant difference between them. 4) There were no differences in blood lactate concentrations between the trained high and low CHO groups immediately after maximal running and during recovery period. 5) Glycogen contents in RV and SM of trained high CHO group were significantly increased, and glycogen contents in RV, WV and liver of trained low CHO group were significantly decreased compared to those of trained mixed diet group. 6) Immediately after maximal running, the blood glucose concentrations of trained high CHO and low CHO groups were $73{\pm}4.0\;and\;67{\pm}6.9mg%$ respecitively. The blood glucose of the trained high CHO group was fully recovered within one hour by feeding. But blood glucose concentration of low CHO group was slowly recovered up to $114{\pm}4.1mg%$ after two hours of feeding and maintained. Those values were still significantly lower than that of trained mixed diet group. The synthetic rates of glycogen in liver and muscles during the recovery period followed the similar time course of the blood glucose recoveries in each group. These results suggest that an increase in MRT of trained high CHO group was attributed to the glycogen supercompensation in slow twitch muscle fibers. And a decrease in MRT of trained low CHO may be due to decreased glycogen contents of liver and muscles. The results also suggest that glycogen supercompensation was more evident in slow twitch red fibers of endurance-trained rats and blood glucose is one of the limiting factors of glycogen synthesis.
L-Serine dehydratase (LSD) is an iron-sulfur containing enzyme that catalyzes the conversion of L-serine to pyruvate and ammonia. Among the bacterial amino acid dehydratases, it appears that only the L-serine specific enzymes utilize an iron-sulfur cluster at their catalytic site. Moreover, bacterial LSDs are classified into four types based on structural characteristics and domain arrangement. To date, only the LSD enzymes from a few bacterial strains have been studied, but more detailed investigations are required to understand the catalytic mechanism of various bacterial LSDs. In this study, LSD type II from Mycobacterium tuberculosis (MtLSD) H37Rv was expressed and purified to elucidate the biochemical and catalytic properties using the enzyme kinetic method. The L-serine saturation curve of MtLSD exhibited a typically sigmoid character, indicating an allosteric cooperativity. The values of $K_m$ and $k_{cat}$ were estimated to be $59.35{\pm}1.23mM$ and $18.12{\pm}0.20s^{-1}$, respectively. Moreover, the plot of initial velocity versus D-serine concentration at fixed L-serine concentrations showed a non-linear hyperbola decay shape and exhibited a competitive inhibition for D-serine with an apparent $K_i$ value of $30.46{\pm}5.93mM$ and with no change in the $k_{cat}$ value. These results provide insightful biochemical information regarding the catalytic properties and the substrate specificity of MtLSD.
Background: The location of parotid gland tumors can influence the duration and the difficulty of the operation. If the information about tumor location was available preoperatively, it would allow accurate operative planning and counseling of patients in terms of the length of the operation and the potential morbidity. Methods: This study was based on a retrospective review of 100 patients with parotid gland tumors underwent parotidectomy from January 2000 to October 2005 at Yong-Dong Severance Hospital. Based on computed tomographic(CT) scan findings, 4 landmarks such as facial nerve(FN) line, Utrecht(U) line, Conn's are(CA), and retromandibular vein (RV) were drawn on the scans in same plane. The location of tumors were determined by the landmarks and confirmed by the operative findings. The accuracy of each landmarks was evaluated. To find out the accuracies according to tumor size, the tumors were divided into 2 groups; less than 2 cm and larger than 2 cm in diameter. Results: U line was the most accurate(94%), sensitive(89.3%) and specific(97.7%) in predicting tumor location of the parotid gland. However, in small tumors less than 2cm, FN line (p=0.022) and RV criteria (p=0.028) were more reliable in accuracy. Conclusion: CA, FN line, U line, and RV are all useful landmarks in preoperative prediction for the location of parotid gland tumors. However, U line was the most accurate, but we must consider that proper landmark should be used in prediction according to the size of tumor because the accuracy of landmark may change.
KSCE Journal of Civil and Environmental Engineering Research
/
v.31
no.6D
/
pp.755-764
/
2011
At the present, planning and installation of parking space sections and lane width are implemented in accordance with the installation standard and parking lot structure by the enforcement regulations of the Parking Act. While large and RV (Recreational Vehicle) vehicles have increased in number recently, parking is complex with improper installation standards. It causes serious problems, including vehicle vandalism, increased civil complaints, travel inconveniences for parking lot users, management and maintenance issues for parking lots, and so forth. The study, therefore, determined that the practical and appropriate realignment of parking unit adjustment and lane width regulations are essential. The study conducted prior review on domestic parking sections and change cases of composition by vehicle types, including the component ratio of domestic and foreign vehicles, width of vehicle by types in order to examine the parking problems that account for a increasing social issues, and provided parking plan methods by types of vehicle, as well as reform and reaction plans for parking unit adjustment and lane width considering security and smooth traffic flow. After comparing and analyzing between estimated installation of parking space and lane width and internal and external cases, the study suggests the improvement that considers expenses and convenience of a driver. Also, it shows installation of parking space that considers traffic safety, improvement of lane width, and parking way depends on vehicle types. By providing estimation for parking planning and parking planning methods that secure safe and smooth traffic flow within a parking lot, the study will contribute to the development of improved alternatives for cost-effective traffic safety and set-up structure, including installation standards of parking lots.
Background: The purpose of this study was to determine the prognostic significance of chest computed tomographic (CT) parameters in acute submassive pulmonary embolism (PE). Methods: Between January 2006 and December 2009, 268 consecutive patients with acute submassive PE that was confirmed by chest CT with pulmonary angiography in emergency room were studied. One experienced radiologist measured CT parameters and judged the presence of right ventricular dysfunction. CT parameters were analyzed to determine their ability to predict a major adverse event (MAE). Results: There were 220 patients included and 61 (27.7%) had MAE. Left ventricular and right ventricular maximum minor axis ($36.4{\pm}8.0$ vs. $41.7{\pm}7.4$, p<0.01; $45.7{\pm}9.4$ vs. $41.5{\pm}7.6$, p<0.01), superior vena cava diameter ($19.2{\pm}3.4$ vs. $18.0{\pm}3.4$, p=0.02), azygos vein diameter ($10.0{\pm}2.2$ vs. $9.2{\pm}2.3$, p=0.02), septal displacement (19 vs. 18, p<0.01) were significantly higher in MAE group than in no MAE group. Patients with MAE had high right ventricular/left ventricular dimension ratio (RV/LV ratio) compared to patients without MAE ($1.34{\pm}0.48$ vs. $1.03{\pm}0.28$, p<0.01). The most useful cut-off value of RV/LV ratio for MAE was 1.3 and the area under the curve was 0.71 (0.62~0.79). Conclusion: RV/LV ratio on chest CT was a significant predictor of submassive PE related shock, intubation, in-hospital mortality, thrombolysis, thrombectomy within 30 days.
Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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v.17
no.4
/
pp.437-445
/
2019
After the permanent shutdown of K1 in 2017, decommissioning processes have attracted great attention. According to the current decommissioning roadmap, the dismantling of the activated components of K1 may start in 2026, following the removal of its spent fuel. Since the reactor vessel (RV) and reactor vessel internal (RVI) of K1 contain massive components and are relatively highly activated, their decommissioning process should be conducted carefully in terms of radiological and industrial safety. For achieving maximum efficiency of nuclear waste management processes for K1, we present activation analysis of the segmentation process and waste classification of the RV and RVI components of K1. For RVI, the active fuel regions and some parts of the upper and lower active regions are classified as intermediate-level waste (ILW), while other components are classified as low-level waste (LLW). Due to the RVI's complex structure and high activation, we suggest various underwater segmentation techniques which are expected to reduce radiation exposure and generate approximately nine ILW and nineteen very low level waste (VLLW)/LLW packages. For RV, the active fuel region and other components are classified as LLW, VLLW, and clearance waste (CW). In this case, we suggest in-situ remote segmentation in air, which is expected to generate approximately forty-two VLLW/LLW packages.
The Sagittarius-Carina spiral arm in the Galaxy contains several massive young open clusters. We present a deep optical photometric study on the massive young open clusters in the Sagittarius-Carina arm, Westerlund 2 and the young open clusters in the ${\eta}$ Carina nebula. Westerlund 2 is a less studied starburst-type cluster in the Galaxy. An abnormal reddening law for the intracluster medium of the young starburst-type cluster Westerlund 2 is determined to be $R_{V,cl}=4.14{\pm}0.08$. The distance modulus is determined from zero-age main-sequence fitting to the reddening-corrected color-magnitude diagrams of the early-type members to be $V_0-M_V=13.9{\pm}0.14mag$. The pre-main sequence (PMS) members of Westerlund 2 are selected by identifying the optical counterparts of X-ray emission sources from the Chandra X-ray observation and mid-infrared emission sources from the Spitzer/IRAC (the Infrared Array Camera) observation. The initial mass function (IMF) shows a slightly flat slope of ${\Gamma}=-1.1{\pm}0.1$ down to $5M_{\odot}$. The age of Westerlund 2 is estimated to be. 1.5 Myr from the main-sequence turn-on luminosity and the age distribution of PMS stars. The ${\eta}$ Carina nebula is the best laboratory for the investigation of the Galactic massive stars and low-mass star formation under the influence of numerous massive stars. We have performed deep wide-field CCD photometry of stars in the ${\eta}$ Carina nebula to determine the reddening law, distance, and the IMF of the clusters in the nebula. We present VRI and $H{\alpha}$ photometry of 130,571 stars from the images obtained with the 4m telescope at Cerro Tololo Inter-American Observatory (CTIO). RV,cl in the η Carina nebula gradually decreases from the southern part (~4.5, around Trumpler 14 and Trumpler 16) to the northern part around Trumpler 15 (~3.5). Distance to the young open clusters in the ${\eta}$ Carina nebula is partly revised based on the zero-age main-sequence fitting to the reddening-corrected color-magnitude diagrams (CMDs) and the (semi-) reddening-independent CMDs. We select the PMS members and candidates by identifying the optical counterparts of X-ray sources from the Chandra Carina Complex Survey and mid-infrared excess emission stars from the Spitzer Vela-Carina survey. From the evolutionary stage of massive stars and PMS stars, we obtain that the northern young open cluster Trumpler 15 is distinctively older than the southern young open clusters, Trumpler 14 (${\leq}2.5 Myr$) and Trumpler 16 (2.5-3.5 Myr). The slopes of the IMF of Trumpler 14, Trumpler 15, and Trumpler 16 are determined to be $-1.2{\pm}0.1$, $-1.5{\pm}0.3$, and $-1.1{\pm}0.1$, respectively. Based on the RV,cl of several young open clusters determined in this work and the previous studies of our group, We suggest that higher RV,cl values are commonly found for very young open clusters with the age of < 4 Myr. We also confirm the correlation between the slope of the IMF and the surface mass density of massive stars.
Purpose: The multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) test developed recently can help detect enteric pathogens of acute gastroenteritis (AGE). This study aimed to investigate the epidemiology of pathogens in children with AGE using the multiplex RT-PCR. Methods: From May 2015 to June 2018, multiplex RT-PCR tests were performed to identify pathogens in the feces of pediatric patients diagnosed with AGE at a secondary hospital in Seoul, Korea. Results: Of the 1,366 stool samples examined for viral pathogens, 483 (35.3%) tested positive for ≥1 pathogen. Group A rotavirus (RV) was detected in 106 cases (7.8%). The positivity rate increased annually from 3.0% (8/263) to 16.7% (48/288) and surged in 2018 (P<0.001). Norovirus (NoV) GII was the most common viral pathogen (263/1,366, 19.3%), and the positivity rate did not increase during the 3 years. Of the 304 stool samples tested for bacterial pathogens, Campylobacter spp. was the most common bacterial pathogen (32/304, 10.5%), followed by Clostridium difficile (22/304, 7.2%) and Salmonella spp. (17/304, 5.6%). The positivity rate of these bacterial pathogens did not change significantly during the study period. Conclusions: NoV GII is the main pathogen in childhood AGE since the introduction of RV vaccine, yet the number of rotavirus-infected patients increased during our study, especially in 2018. Therefore, further research is needed including the possibility of emergence of novel RV strains. Campylobacter spp. is the predominant cause of bacterial AGE in children. For proper treatment, the clinical characteristics of the bacteria should be taken into consideration, and continuous monitoring is necessary.
Recent automobile manufacturing technology has improved not only the function and performance of cars, but also the audio systems in cars so as to increase their marketability. Automobile manufacturers always have the option of simply installing an expensive acoustic system to help customers enjoy a high-level sound quality car audio system. However, this also tends to increase the MSRP (Manufacturer's Suggested Retail Price) of the car. Therefore, it is desirable, where possible, to enhance the sound quality of plainer, less expensive audio devices to help customers feel as if they have a high-quality and expensive audio device in their car. In order to make this happen, the manufacturer must develop an optimal interior environment and audio system at a relatively lower cost. To this end, features of the car audio system can be enhanced by analyzing audio frequency response and using performance metrics to figure out the characteristics of the human auditory system. This study analyzed the sound field of Korean Recreation Vehicles (RVs) using the Internet of Things (IoT) sensor for the measurement of car audio system. As a result, high energy of sensitive bandwidth, one of the human auditory characteristics often makes annoying sound. This study also found that increasing the frequency response flatness is required by taking human auditory field into account when designing the car audio system for the future.
This study makes an empirical comparison of various realized volatilities (RVs) in terms of overnight returns. In financial asset markets, during overnight or holidays, no or few trading data are available causing a difficulty in computing RVs for a whole span of a day. A review will be made on several RVs reflecting overnight return variations. The comparison is made for forecast accuracies of several RVs for some financial assets: the US S&P500 index, the US NASDAQ index, the KOSPI (Korean Stock Price Index), and the foreign exchange rate of the Korea won relative to the US dollar. The RV of a day is compared with the square of the next day log-return, which is a proxy for the integrated volatility of the day. The comparison is made by investigating the Mean Absolute Error (MAE) and the Root Mean Square Error (RMSE). Statistical inference of MAE and RMSE is made by applying the model confidence set (MCS) approach and the Diebold-Mariano test. For the three index data, a specific RV emerges as the best one, which addresses overnight return variations by inflating daytime RV.
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