• 한국정보전자통신기술학회논문지
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• 제4권4호
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• pp.286-294
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• 2011

본 논문은 ITU-T 권고안 J-38 부록 B에 명시된 전송방식의 분석 및 시뮬레이션을 토대로 성능을 분석 하였으며 FPGA 구현시 야기되는 문제점을 나타내고, 해결방안을 제시하였다. 구현상의 문제점으로는 크게 두가지로 분류되는데, 첫째로 다양한 부호화 방식과 변조방식 그리고 심볼 단위 및 비트 단위의 처리로 인해 많은 클럭수를 요구하는데 본 논문에서는 read/write 메모리를 이용하여 필요한 클럭수를 줄였다. 둘째로는 펑쳐링 부호화된 TCM 복호기에 펑처링 패턴에 정확한 동기를 얻지 못하면 프레임 동기 심볼인 UW(Unique sync-Word)를 획득하지 못한다. 따라서 본 논문에서는 펑처링 패턴과 UW 심볼의 동기를 맞추는 알고리즘을 제시하였다. 이러한 알고리즘 분석 및 구현상의 문제점 해결을 토대로 본 논문에서는 ITU-T J38 annex B의 하향 스트림 채널 부호화 시스템을 VHDL 언어를 사용하여 FPGA 칩에 직접 구현하였다.

• 한국정보통신학회논문지
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• 제21권12호
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• pp.2394-2402
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• 2017

수심이 얕은 연안에서는 다중경로에 의한 주파수 선택적인 페이딩이 수중음향통신시스템의 성능을 결정한다. 본 연구에서는 수심 약 50m이고 유효파고 0.5m, 사니질 저질인 부산인근 해역의 수중음향통신 채널의 특성과 다중반송파 음향통신시스템의 성능을 평가하였다. 송수신기 수평거리에 따른 다중경로 지연 확산과 시간영역 및 주파수 영역 페이딩 특성을 제시하고 전송률 1kbps인 5ch-4FSK 시스템의 비트 오류율을 평가하였다. 시간영역 페이딩 오류를 제거하기 위해 리드 솔로몬 코드를 적용하였다. 다중경로는 4개 이하로 구성되며 송수신기 거리가 증가하면 시간 및 주파수 영역 페이딩은 작아지며 시스템의 비트 오류율은 감소하고 600m 이상의 거리에서 비트 오류율은 약 10-4 이었다.

• IMMUNE NETWORK
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• 제5권4호
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• pp.205-214
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• 2005

Background: We examined global gene expression profiles of peripheral blood mononuclear cells (PBMCs) in patients with ulcerative colitis (DC), and tested whether the identified genes with the altered expression might be associated with susceptibility to UC. Methods: PBMCs from 8 UC and 8 normal healthy (NH) volunteers were collected, and total RNAs were subjected to the human 8.0K cDNA chip for the micro array analysis. Real time-PCR (RT-PCR) was performed to verify the results of micro array. One hundred forty UC patients and 300 NH controls were recruited for single nucleotide polymorphism (SNP) analysis. Results: Twenty-five immune function-related genes with over 2-fold expression were identified. Of these genes, two chemokines, namely, CXCL1 and CCL20, were selected because of their potential importance in the evocation of host innate and adaptive immunity. Four SNPs were identified in the promoter and coding regions of CXCL1, while there was no significant difference between all patients with UC and controls in their polymorphisms, except minor association at g.57A>G (rs2071425, p=0.02). On the other hand, among three novel and one known SNPs identified in the promoter region of CCL20, g. -1,706 G>A (p=0.000000055), g. -1,458 G>A (p=0.0048), and g. -962C>A (p=0.0006) were found to be significantly associated with the susceptibility of Uc. Conclusion: Altered gene expression in mononuclear cells may contribute to IBD pathogenesis. Although the findings need to be confirmed in other populations with larger numbers of patients, the current results demonstrated that polymorphisms in the promoter region of CCL20 are positively associated with the development of Uc.

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1529-1536
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• 2006

We cloned a gene of ompH(D:4) from pigs infected with P. multocida D:4 in Korea [16]. The gene is composed of 1,026 nucleotides coding 342 amino acids (aa) with a signal peptide of 20 aa (GenBank accession number AY603962). In this study, we analyzed the ability of the ompH(D:4) to induce protective immunity against a wild-type challenge in mice. To determine appropriate epitope(s) of the gene, one full and three different types of truncated genes of the ompH(D:4) were constructed by PCR using pET32a or pRSET B as vectors. They were named ompH(D:4)-F (1,026 bp [1-1026] encoding 342 aa), ompH(D:4)-t1 (693 bp [55-747] encoding 231 aa), ompH(D:4)-t2 (561 bp [187-747] encoding 187 aa), and ompH(D:4)-t3 (540 bp [487-1026] encoding 180 aa), respectively. The genes were successfully expressed in Escherichia coli BL21(DE3). Their gene products, polypeptides, OmpH(D:4)-F, -t1, -t2, and -t3, were purified individually using nickel-nitrilotriacetic acid (Ni-NTA) affinity column chromatography. Their $M_rs$ were determined to be 54.6, 29, 24, and 23.2 kDa, respectively, using SDS-PAGE. Antisera against the four kinds of polypeptides were generated in mice for protective immunity analyses. Some $50{\mu}g$ of the four kinds of polypeptides were individually provided intraperitoneally with mice (n=20) as immunogens. The titer of post-immunized antiserum revealed that it grew remarkably compared with pre-antiserum. The lethal dose of the wild-type pathogen was determined at $10{\mu}l$ of live P. multocida D:4 through direct intraperitoneal (IP) injection, into post-immune mice (n=5, three times). Some thirty days later, the lethal dose ($10{\mu}l$) of live pathogen was challenged into the immunized mouse groups [OmpH(D:4)-F, -t1, -t2, and -t3; n=20 each, two times] as well as positive and negative control groups. As compared within samples, the OmpH(D:4)-F-immunized groups showed lower immune ability than the OmpH(D:4)-t1, -t2, and -t3. The results show that the truncated-OmpH(D:4)-t1, -t2, and -t3 can be used for an effective vaccine candidate against swine atrophic rhinitis caused by pathogenic P. multocida (D:4) isolated in Korea.