• Reproductive and Developmental Biology
• /
• v.35 no.3
• /
• pp.209-214
• /
• 2011

Although the function and utility of RNA transcripts derived from matured spermatozoa remains unclear, they might play important roles in the establishment of a paternal genome and subsequently embryo development. Herein, we investigated the expression of X-chromosome linked RNA transcripts in matured bovine spermatozoa. The total RNA was extracted from the matured spermatozoa, and then converted to cDNA. Autosomal genes (ACT-${\beta}$ and H-2A) and X-chromosome linked genes (ANT3, HPRT, MeCP2, RPS4X, XIAP, XIST and ZFX) were analyzed for the characterization of X-chromosome linked RNA transcripts and compared to female fibroblasts by RT-PCR. The transcripts of autosomal genes (ACT-${\beta}$ and H2A) and X-chromosome linked genes (ANT3, HPRT, MeCP2, RPS4X and ZFX) were not detected in spermatozoa. However, XIAP (X-linked inhibitor of apoptosis protein) and XIST (X-chromosome inactive-specific transcript, a kind of paternal imprinted gene) transcripts were detected in spermatozoa, and relative levels of XIAP and XIST transcripts were similar and 0.5-fold lower when compared to female fibroblasts, respectively. Based on the findings, it is summarized that the presence of RNA transcripts of XIAP and XIST in the isolated spermatozoa may imply their role in inhibition of apoptosis and induction of X-chromosome inactivation in embryo development.

• Korean Journal of Microbiology
• /
• v.30 no.2
• /
• pp.101-107
• /
• 1992

Cytochrome c oxida5e from chemotrophically grown R p , geliitinosu was purified by cytochrome c affinity chromatography and DEAE-Sephacel ion exchange chromatography. The molecular weight of the cytochrome c oxidase was approximately 110.000 Da by sephacryl s-300 gel chromatography and approximately 52, 000 Da by SDS-gel electrophoresis, respectively. Therefore. cytochrolne c oxidase of Rps. gehtinosu seems to be dimer. The cytochrome c oxidasc was very sensitive to temperature. It's Km and Vmax were 20 pM and 44 unitlmg protein for horsc heart cytochrome c as a substrate. respectively, and its optimum pH and temperature were 6.4 and 25$^{\circ}$C. respectively. The absorption peaks of the reduced cytochrome c oxidase showed at 554 nm, 523 nm. and 422 nm. The activiiy of cytochrome c oxidase was inhibited by KCN, and NaN3, but not by CO, antimycir~ A. and myxothiazol. The cytochrome c-551 was produced either in phototrophically or chemotrophically grown Rps. gelaiinosci. The rcduced cytochrome c-551 was oxidized by b-type cytochrome c oxidase from Rp.v. gc.lrtino.sc~. Km and Vmax of cytochrome c oxidase was 26 pM and 31 unitlnlg protein For cytochrome c-551 as a substrate. respectively. Thercfore. thc electron transfer chain of chemotrophically grown Rps. glatinosa seems lo be ubiquinol cytochrome bc, complex -'cytochrome c-55lMb-type cytochrome c oxidase+02.

• The Plant Pathology Journal
• /
• v.28 no.2
• /
• pp.207-211
• /
• 2012

It has been known that streptomycin resistance in bacteria can occur as a results of chromosomal mutation or through gene acquisition or both. Chromosomal mutations for resistances are point mutations in the rpsL gene, which alter ribosomal protein S12. Acquired resistance has occurred when an $Sm^R$ plasmid carrying transposon Tn5393 with tandem strA-strB gene is transferred by conjugation. A total of 686 isolates of Xanthomonas smithii subsp. citri causal agent of citrus canker disease were collected from 26 citrus orchards in Jeju Island in 2003 and 2004 seasons. Forty-nine of 111 isolates from streptomycin non-sprayed orchards in 2003 season were resistant to streptomycin. Of 107 isolates from orchards sprayed one time with streptomycin, 58 isolates were resistant, and 166 of 221 isolates from orchards sprayed two times with streptomycin were resistant. In 12 orchards sprayed three or more times with streptomycin, 219 of 247 isolates were resistant to streptomycin. Twenty-five isolates of X. smithii subsp. citri were surveyed to identify the mechanisms of streptomycin resistance in this study. Twenty-one of these 25 isolates were resistant to streptomycin, and it was proven by PCR assay that 18 of the 21 streptomycin resistant isolates have the strB gene. In sixteen of the 21 streptomycin resistant isolates, it was occurred a point mutation altered codon lysine (AAG)-41 of rpsL gene to arginine (AGG). The streptomycin-sensitive isolates easily acquired the resistance by mixed culture with resistant isolates. The strB gene was amplified from the isolates that acquired the resistance by mixed culture, and one isolate of them was also point-mutated in codon 41 of rpsL gene to be resistant. In this study, most of the streptomycin-resistant isolates of X. smithii sub sp. citri in Jeju island expressed the resistance by both chromosomal point mutation and gene acquisition, and the resistance was easily acquired through conjugation by culture mixed with streptomycin resistant and sensitive strains.

• Journal of Technology Innovation
• /
• v.27 no.2
• /
• pp.1-36
• /
• 2019

In line with the international community's movement to reduce greenhouse gas emission, Korea implemented FIT(Feed in Tariff) in 2002 as part of its renewable energy development project. Although the policy had shifted to full-scale RPS(Renewable Portfolio Standards) in 2012, policymakers are still seeking changes due to policy ineffectiveness. While previous studies explain sudden policy changes through external factors, recent research sheds light on internal factors in the process of policy transition. The purpose of this study is to investigate the factors that are responsible for rapidly changing policies in latecomer nations. In order to find this, we look at the case of transition from the FIT to the RPS in Korea's expansion of renewable energy policy. As a result of the research, it is confirmed that the Top-Down decision making system of Korea and the external regulatory change cause rapid policy transition. By looking at these variables, we propose useful implications for policymakers to minimize the policy failure in future policy design and evolution.

• Journal of the Korean Society of Propulsion Engineers
• /
• v.5 no.2
• /
• pp.65-72
• /
• 2001

In order to improve the performance of a free power turbine type gas turbine engine by injecting the atomized water into a compressor inlet., a study on Moisture Air Turbine (MAT) cycle was proposed. Compressor work by air-water mixtures in phase change was theoretically considered, and it was found that the water evaporation might reduce the compressor work. Cycle model calculations predicted that power increments of 16.2%, 14.9% and 12.6% by 1.0% water to the air flow rate at the compressor intake with rotational shaft speeds of 1000, 1210, 1350 rps were obtained, and also thermal efficiency due to the reduction of compressor work was improved.

• Journal of fish pathology
• /
• v.26 no.3
• /
• pp.231-240
• /
• 2013

Adjuvants are immune enhancers that are often used in vaccination to augment the immune response of a vaccine, thereby enhancing the protective immunity against the targeted disease. In the present study, we used the recombinant protein, such as rRbCC1, this protein was produced from rock bream CC chemokine 1. To verify the adjuvant effects of this recombinant protein, the immune responses of rock bream to Streptococcus iniae (S. iniae) FKC vaccination, which alone or in combination with recombinant protein was analyzed and then also performed experimental challenge with live S. iniae. The result of serum agglutination titres was showed relatively low levels however, the efficacy of FKC vaccine still conferred protection against S. iniae. Moreover, the adverse effects result showed that no statistically significant difference was revealed between high concentration injected and non-injected fish groups, generally. The relative percent survival (RPS) of FKC + recombinant vaccination group was significantly higher than that of vaccinated group with FKC alone. After experimental challenge to the rock bream by injection with live bacteria (S. iniae), the FKC + rRbCC1 vaccination group was showed 87.0% RPS, however, the RPS of FKC alone vaccination was 68.2%. The results indicated that the recombinant protein as an adjuvant had a clear synergism to injection vaccine of rock bream.

• Proceedings of the Zoological Society Korea Conference
• /
• 2000.10a
• /
• pp.260.2-261
• /
• 2000

No Abstract, See Full Text

• Nuclear Engineering and Technology
• /
• v.39 no.2
• /
• pp.133-142
• /
• 2007

The Electric Power Research Institute (EPRI) demonstrated a method for monitoring the performance of instrument channels in Topical Report (TR) 104965, 'On-Line Monitoring of Instrument Channel Performance.' This paper presents the results of several models originally developed by EPRI to monitor three nuclear plant sensor sets: Pressurizer Level, Reactor Protection System (RPS) Loop A, and Reactor Coolant System (RCS) Loop A Steam Generator (SG) Level. The sensor sets investigated include one redundant sensor model and two non-redundant sensor models. Each model employs an Auto-Associative Kernel Regression (AAKR) model architecture to predict correct sensor behavior. Performance of each of the developed models is evaluated using four metrics: accuracy, auto-sensitivity, cross-sensitivity, and newly developed Error Uncertainty Limit Monitoring (EULM) detectability. The uncertainty estimate for each model is also calculated through two methods: analytic formulas and Monte Carlo estimation. The uncertainty estimates are verified by calculating confidence interval coverages to assure that 95% of the measured data fall within the confidence intervals. The model performance evaluation identified the Pressurizer Level model as acceptable for on-line monitoring (OLM) implementation. The other two models, RPS Loop A and RCS Loop A SG Level, highlight two common problems that occur in model development and evaluation, namely faulty data and poor signal selection

• Proceedings of the Korean Society of Propulsion Engineers Conference
• /
• 2001.04a
• /
• pp.54-58
• /
• 2001

In order to Improve the performance of a free power turbine type gas turbine engine by injecting the atomized water into a compressor inlet., a study on Moisture Air Turbine (MAT) cycle was proposed. Compressor work by air-water mixtures in phase change was theoretically considered, and it was found that the water evaporation might reduce the compressor work. Cycle model calculations predicted that power increments of 21.7%, 20.2% and 18.4% by 1.5% water to the air flow rate at the compressor intake with rotational shaft speeds of 1000, 1210, 1350 rps were obtained, and also thermal efficiency due to the reduction of compressor work was improved.

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 1996.06c
• /
• pp.519-527
• /
• 1996

Most operation related to the application granular agrochemicals in korea are manual and labour consuming. As a multipurpose granular applicator, a pneumatic granular applicator that can be attached to conventional ride -on machines for paddy field was designed in this study. Experiments and simulations were carried out for determine in the design factors with one fertilizer (DongBu 21-17-17) and one pesticide (SamKong Ivi). The maximum terminal velocity of granular chemicals was estimated as 14.2m/s. A better distribution pattern was obtained at the diffuser having only deflectors without dividing guides. The diffuser spacing 1.0m for the fertilizer and the diffuser spacing 0.8m for the pesticide at the boom height over 0.80m were acceptable for the uniform distribution. In the case that the forward speed of ride-on machines was 0.7m/s, the groove opening and the roller speed of the selected metering device were13mm -15mm at 2 rps for the fertilizer, and 9 mm-11mm at 0.25rps or the pesticide. This study suggested a method of developing a pneumatic applicator for granular agrochemicals.