• Title/Summary/Keyword: RP4-mobilising systme

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Isolation of Nif$^{-10}$ -mutants through transposon mutagenesis in enterobacter agglomerans 339 (Enterobacter agglomerans 339에 있어서 transposon umtagenesis를 통한 Nif$^{-10}$ -mutants 분리 동정)

  • 민병환;이호자
    • Korean Journal of Microbiology
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    • v.26 no.1
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    • pp.20-26
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    • 1988

  • Three $NIf^{-}$ -mutants were isolated from Enterbacter agglomerans 339 through the transposon umtagenesis using a RP4-mobilising system for its nif-gene characterization. All mutants hadn't acetylene-reduction ability. Then we confirmed that Tn5 was inserted into all conserved nif-plasmids through the Southern Hybridization.

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