• Sleep Medicine and Psychophysiology
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• v.23 no.1
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• pp.25-28
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• 2016

Objectives: The retinoid-related orphan receptor A (RORA) gene has been reported to have an impact on circadian rhythm regulation. In this study, we analyzed the relationship between the RORA gene polymorphism and diurnal preference in Korean young adults. Methods: A population of 504 young adults was included in the study. All subjects were given and completed a 13-item composite scale for morningness (CSM). The RORA gene rs11071547 single-nucleotide polymorphism (SNP) was genotyped by PCR-based methods. Results: CSM score was not associated with genotype or allele carrier status of the RORA rs11071547 SNP. Conclusion: This result indicates that the RORA rs11071547 SNP does not play a role in diurnal preference.

• Sleep Medicine and Psychophysiology
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• v.20 no.2
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• pp.63-68
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• 2013

Objectives: Several evidence has been suggested that the circadian gene variants contribute to the pathogenesis of seasonal affective disorder. In this study, we aimed to investigate the polymorphism in RORA (Retinoid-related orphan receptor A) gene in relation to seasonal variations among healthy young adults in Seoul, Korea. Methods: A total of 507 young healthy adult subjects were recruited by advertisement. Seasonal variations were assessed by the Seasonality Pattern Assessment Questionnaire (SPAQ). Single-nucleotide polymorphism in the RORA rs11071547 gene was genotyped by PCR in 507 individuals. Considering summer type as confounding factor, we conducted analysis 478 subjects except 29 subjects of summer type. The Chi-square test was conducted to compare differences between groups of seasonals and non-seasonals. Association between genotypes and Global Seasonality Score (GSS) were tested using ANCOVA (Analysis of covariance). Results: In this sample, the prevalence of SAD was 12.1% (winter type 9.3%, summer type 2.8%). There is no significant difference in genotyping distribution of RORA rs11071547 between groups of seasonals and non-seasonals. Global seasonality score (GSS) and scores of all subscales except body weight and appetite were not significantly different between the group with C allele homozygote and the group with T allele homozygote and heterozygote (p-value 0.138). Scores of body weight and appetite were significantly higher in group with C allele homozygotes. Conclusion: These results suggest that RORA gene polymorphism play a role in seasonal variations in appetite and body weight and is associated with susceptibility to seasonal affective disorder in some degree in the population studied.

• BMB Reports
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• v.43 no.2
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• pp.122-126
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• 2010

Homozygous staggerer ($RORa^{sg/sg}$) mice showed a severe ataxia caused by cerebellum degeneration. Decreased and dysfunctional Rora is a main cause of this neurologic phenotype. The phenotype of staggerer mice has been well known in cerebellum. However, there has been rarely reported about cerebrum even though of staggerer is expressed in merely cerebellum but hippocampus, thalamus, cortex, and olfactory bulb. The expressions of Ki67, doublecortin (DCX), and NeuN, which are cell proliferation, neuronal differentiation and mature neuron markers, respectively, were measured with immunohistechemistry in dentate gyrus in staggerer mice in order to uncover whether staggerer can affect the change in dentate gyrus. The immunoreactivities of DCX and NeuN were significantly reduced in the dentate gyrus of staggerer mice than normal control, while Ki67 were rarely unchanged in staggerer mice. These results suggest that staggerer mutation has an influence on the neuronal differentiation and development not only in cerebellum but also in dentate gyrus.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.375-375
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• 2011

유역에서 강우에 따라 유출이 발생하면 지표면을 따라 하천으로 유입되거나, 땅속으로 침투하여 깊은 대수층으로 유입되던지 기저유출 형태로 하천으로 유입된다. 이렇듯 하천을 구성하는 중요 두 가지 요소인 직접유출량과 기저유출량을 정확히 산정하는 것이 유역 수자원관리 및 비점오염원 관리에 매우 중요한 부분이라 할 수 있다. 그동안 하천에서 측정된 유출량에서 직접유출과 기저유출을 분리하기 위한 많은 연구가 진행되어 왔으며, 최근에는 주관적인 면을 배제하고 장기실측 유량자료를 이용하여 기저유출을 분리할 수 있는 BFlow, HYSEP, PART, RORA, RECESS, 디지털 필터링 모형 등 많은 프로그램들이 개발되어 활용되어 오고 있다. 또한 최근에는 인터넷을 통해 활용할 수 있는 Web 기반 WHAT 프로그램이 개발되어 전 세계적으로 널리 활용되고 있다. 이에 본 연구에서는 XML 프로그래밍 기법을 이용하여 WHAT 프로그램을 확장한 Expaned XML-based WHAT (EX-WHAT) 시스템 (http://www.EnvSys.co.kr/~exwhat)을 개발 하였다. 기존의 시스템에서는 USGS 일유량자료를 URL을 통해 WHAT 서버에 저장한 후 이를 가공하여 수문 분석을 수행하였으나, 이번 연구를 통해서 개발된 시스템은 XML/Parser를 이용하여 USGS 서버에 저장되어 있는 일유량자료를 바로 읽어서 수문분석을 수행할 수 있게 되었다. 이 EXWHAT 시스템에는 BFlow, HYSEP, PART, Digital Filters 와 같은 엔진이 사용되고 있다. 본 연구에서 개발된 EX-WHAT 분석결과는 XML 형식으로 제공되고 있기 때문에, 다른 Web/Desktop 기반의 관련 프로그램에서 바로 활용될 수 있을 것이라 판단된다. 특히 EX-WHAT 분석결과는 유역관리, 기저유출을 통한 비점오염원 관리 평가, 지속가능한 지하수 고나리 등 다양한 수문/비점오염 연구/실무에 활용될 수 있을 것이라 판단된다.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2003.10a
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• pp.159-159
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• 2003

Comet assay is a potential monitoring tool because DNA strand breakage may be produced by a wide range of agents. The comet assay, also called the single-cell gell electrophoresis (SCGE) assay, is rapid and sensitive method for the detection of DNA damage in cells. This study was performed for the identification of DNA damage in the cells from flounders and clams exposed to PAHs. As a control experiments, flounder and clam cells were exposed to $H_2O$$_2$. The cells exposed to $H_2O$$_2$ were displayed a typical nuclei movement DNA damage of cells were significantly increased when the isolated cells from the blood of flounders and the tissue of clams were in vitro exposed to the different concentrations (5, 10, 50, 100 ppb) of five kinds of PAHs (benzo[a]pyrene, pyrene, fluoranthene, anthrancene, and phenanthrene). For the in vivo test, flounders and clams were exposed to the different concentrations of BaP for 4 days. The results showed that DNA strand breakage was effected by the concentration of BaP and the duration of exposure. In high concentration of BaP, the mean tail lengths of nuclei was longer than it In low concentration, while the mean size of head DNA decreased. In this research, both in vitro and in vivo genotoxicity of PAHs could be biomonitored by the comet assay. Especially, clams and flounders seem to be useful as materials for monitoring genotoxic damage by comet assay.

• Environmental Analysis Health and Toxicology
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• v.20 no.1
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• pp.67-73
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• 2005

오염물질의 노출에 의해 발현이 변화되는 유전자의 발굴은 외부환경 자극에 대한 적응이나 반응의 메커니즘을 알아내는뎨 중요한 정보를 제공하며, 오염물질에 반응하는 유전자는 환경오염을 감지하는 분자 마커로 개발될 수 있다. DD-PCR 기법은 차등 발현 유전자들을 발굴해내기 위한 유용한 방법으로 사용되어 왔고, 본 연구는 이 방법을 이용하여 벤조피렌에 반응하는 조피볼락 유전자들의 발굴을 목적으로 진행되었다. 간조직에서 추출한 RNA로부터 벤조피렌의 노출에 의해 발현 양이 달라진 12개의 클론을 발굴하였고, 그 염기서 열을 분석하였다. 또한 벤조피렌의 노출시간을 각각 6, 12, 24시간으로 달리한 조피볼락에서 12개의 클론 중 4개의 클론에 대해 northern blot 분석이 실시되었으며, 이들 모두 노출시간에 따 라 발현양이 증가 또는 감소하는 것이 확인되었다. 본 연구결과는 오염물질의 영향에 의한 유전자들의 발현에 관한 전반적인 지식을 제공하였고, 나아가 환경오염이나 외부 스트레스를 감지해 낼 수 있는 바이오마커의 개발을 위한 첫 단계로서의 정보를 제공할 수 있을 것으로 생각된다.