• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2005년도 추계종합학술대회
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• pp.525-527
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• 2005

인터넷 서비스가 발달함에 따라 다자간 화상회의 등 다수의 송신자를 가지는 멀티캐스트 서비스에 대한 관심이 증가하고 있다. 다자간 멀티캐스트 서비스를 지원하기 위해서는 프로토콜뿐만 아니라 가입자 관리 및 세션 관리를 제공하는 세션 관리자의 기능이 요구된다. 현재 ITU-T에서 표준화를 진행 중인 RMCP-3는 다대다 멀티캐스트 프로토콜로써, 다수의 참여자들이 송신자 역할을 수행하여 데이터를 송수신하는 기능을 제공한다. 본고는 컨퍼런스 응용과 같이 다수의 호스트가 데이터를 생성하여 보낼 수 있는 다자간 멀티캐스트 통신 프로토콜에서의 세션 관리자 기능에 대한 고려사항을 살펴보고자 한다.

• IMMUNE NETWORK
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• 제10권1호
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• pp.15-25
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• 2010

Background: Rat mast cells were regarded as a good model for mast cell function in immune response. Methods: Rat bone marrow mast cells (BMMC) were prepared both by recombinant rat IL-3 (rrIL-3) and by recombinant mouse stem cell factor (rmSCF), and investigated for both proliferation and differentiation in time course. Rat BMMC was induced by culture of rat bone marrow cells (BMCs) in the presence of both rrIL-3 (5 ng/ml) and rmSCF (5 ng/ml). Culture media were changed 2 times per week with the cell number condition of $5{\times}10^4/ml$ in 6 well plate. Proliferation was analyzed by cell number and cell counting kit-8 (CCK-8) and differentiation was by rat mast cell protease (RMCP) II and histamine. Results: Cell proliferation rates reached a maximum at 8 or 11 days of culture and decreased thereafter. However, both RMCP II production and histamine synthesis peaked after 11 days of culture. By real time RT-PCR, the level of histidine decarboxylase mRNA was more than 500 times higher on culture day 11 than on culture day 5. By transmission electron microscopy, the cells were heterogeneous in size and contained cytoplasmic granules. Using gated flow cytometry, we showed that cultured BMCs expressed high levels of $Fc{\varepsilon}RI$ and the mast cell antigen, ganglioside, on culture day 11. Conclusion: These results indicate that rat BMMCs were generated by culturing BMCs in the presence of rrII-3 and rmSCF and that the BMMCs have the characteristics of mucosal mast cells.