• BMB Reports
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• v.51 no.10
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• pp.484-485
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• 2018

Receptor-interacting protein kinase-3 (RIP3 or RIPK3) is a serine-threonine kinase largely essential for necroptotic cell death; it also plays a role in some inflammatory diseases. High levels of RIP3 are likely sufficient to activate necroptotic and inflammatory pathways downstream of RIP3 in the absence of an upstream stimulus. For example, we have previously detected high levels or RIP3 in the skin of Toxic Epidermal Necrolysis patients; this correlates with increased phosphorylation of MLKL found in these patients. We have long surmised that there are molecular mechanisms to prevent anomalous activity of the RIP3 protein, and so prevent undesirable cell death and inflammatory effects when inappropriately activated. Recent discovery that Carboxyl terminus of Hsp 70-Interacting Protein (CHIP) could mediate ubiquitylation- and lysosome-dependent RIP3 degradation provides a potential protein that has this capacity. However, while screening for RIP3-binding proteins, we discovered that pellino E3 ubiquitin protein ligase 1 (PELI1) also interacts directly with RIP3 protein; further investigation in this study revealed that PELI1 also targets RIP3 for proteasome-dependent degradation. Interestingly, unlike CHIP, which targets RIP3 more generally, PELI1 preferentially targets kinase active RIP3 that has been phosphorylated on T182, subsequently leading to RIP3 degradation.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7069-7074
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• 2013

Programmed cell death is a basic cellular process that is critical to maintaining tissue homeostasis. In contrast to apoptosis, necrosis was previously regarded as an unregulated and uncontrollable process. However, as research has progressed, necrosis, also known as necroptosis or programmed necrosis, is drawing increasing attention, not least becasu of its possible impications for cancer research. Necroptosis exhibits a unique signaling pathway that requires the involvement of receptor interaction protein kinases 1 and 3 (RIP1 and RIP3), mixed lineage kinase domain-like (MLKL), and phosphoglycerate mutase 5 (PGAM5) and can be specifically inhibited by necrostatins. Not only does necroptosis serve as a backup cell death program when apoptosis is inhibited, but it is now recognized to play a pivotal role in regulating various physiological processes and the pathogenesis of a variety of human diseases such as ischemic brain injury, immune system disorders and cancer. The control of necroptosis by various defined trigger factors and signaling pathways now offers the opportunity to target this cellular process for therapeutic purposes. The purpose of this paper is to review current findings concerning the connections between various trigger factors and the RIP1/RIP3 signaling pathway as it relates to necroptosis.

• BMB Reports
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• v.48 no.6
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• pp.303-312
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• 2015

Receptor-interacting protein kinase-3 (RIP3, or RIPK3) is an essential protein in the "programmed", or "regulated" necrosis cell death pathway that is activated in response to death receptor ligands and other types of cellular stress. Programmed necrotic cell death is distinguished from its apoptotic counterpart in that it is not characterized by the activation of caspases; unlike apoptosis, programmed necrosis results in plasma membrane rupture, thus spilling the contents of the cell and triggering the activation of the immune system and inflammation. Here we discuss findings, including our own recent data, which show that RIP3 protein expression is absent in many cancer cell lines. The recent data suggests that the lack of RIP3 expression in a majority of these deficient cell lines is due to methylation-dependent silencing, which limits the responses of these cells to pro-necrotic stimuli. Importantly, RIP3 expression may be restored in many cancer cells through the use of hypomethylating agents, such as decitabine. The potential implications of loss of RIP3 expression in cancer are explored, along with possible consequences for chemotherapeutic response. [BMB Reports 2015; 48(6): 303-312]

• Nuclear Engineering and Technology
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• v.31 no.3
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• pp.257-266
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• 1999

A simplified statistical methodology is developed in order to both reduce over-conservatism of deterministic methodologies employed for PWR fuel rod internal pressure (RIP) calculation and simplify the complicated calculation procedure of the widely used statistical methodology which employs the response surface method and Monte Carlo simulation. The simplified statistical methodology employs the system moment method with a deterministic approach in determining the maximum variance of RIP The maximum RIP variance is determined with the square sum of each maximum value of a mean RIP value times a RIP sensitivity factor for all input variables considered. This approach makes this simplified statistical methodology much more efficient in the routine reload core design analysis since it eliminates the numerous calculations required for the power history-dependent RIP variance determination. This simplified statistical methodology is shown to be more conservative in generating RIP distribution than the widely used statistical methodology. Comparison of the significances of each input variable to RIP indicates that fission gas release model is the most significant input variable.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.2277-2284
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• 2016

Ribosome-inactivating protein (RIP) from Mirabilis jalapa L. leaves has cytotoxic effects on breast cancer cell lines but is less toxic towards normal cells. However, it can easily be degraded after administration so it needs to be formulated into nanoparticles to increase its resistance to enzymatic degradation. The objectives of this study were to develop a protein extract of M. jalapa L. leaves (RIP-MJ) incorporated into nanoparticles conjugated with Anti-EpCAM antibodies, and to determine its cytotoxicity and selectivity in the T47D breast cancer cell line. RIP-MJ was extracted from red-flowered M. jalapa L. leaves. Nanoparticles were formulated based on polyelectrolyte complexation using low viscosity chitosan and alginate, then chemically conjugated with anti-EpCAM antibody using EDAC based on carbodiimide reaction. RIP-MJ nanoparticles were characterised for the particle size, polydispersity index, zeta potential, particle morphology, and entrapment efficiency. The cytotoxicity of RIP-MJ nanoparticles against T47D and Vero cells was then determined with MTT assay. The optimal formula of RIP-MJ nanoparticles was obtained at the concentration of RIP-MJ, low viscosity chitosan and alginate respectively 0.05%, 1%, and 0.4% (m/v). RIP-MJ nanoparticles are hexagonal with high entrapment efficiency of 98.6%, average size of 130.7 nm, polydispersity index of 0.380 and zeta potential +26.33 mV. The $IC_{50}$ values of both anti-EpCAM-conjugated and non-conjugated RIP-MJ nanoparticles for T47D cells (13.3 and $14.9{\mu}g/mL$) were lower than for Vero cells (27.8 and $33.6{\mu}g/mL$). The $IC_{50}$ values of conjugated and non-conjugated RIP-MJ for both cells were much lower than $IC_{50}$ values of non-formulated RIP-MJ (>$500{\mu}g/mL$).

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.15 no.3
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• pp.110-114
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• 2010

A data set of current, wind and wave height measured at the monitoring buoy and sea level at Busan harbor were analyzed to explain the physical conditions during the strong rip current events at Haeundae Beach of Suyeong Bay during 13~15 August 2009. Tidal current, with spring-neap variations, has similar average speed to the short-term non-tidal currents. The common features at the time of rip currents are the strong northeasterly wind and superposition of tidal and non-tidal currents both flowing toward the coast. However on 14 August when the rip current did not occur, tide and wave height were similar to the rip-current cases but the tidal and non-tidal current were to nearly opposite directions. While strong winds produce large waves thus the basic condition for rip current but its influence on the local circulation in the bay is relatively small. Of the three adjacent beaches, only at Haeundae the rip currents are reported. This difference may be due to the unique bottom topography featured by underwater hill in the central region off Haeundae which can decay the incoming waves, tides and currents to intensify the rip current.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.31 no.6
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• pp.485-492
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• 2019

The observation system has been developed to investigate the rip currents at Haeundae beach using X-band marine radar. X-band radar system can observe shape, size, and velocity of rip currents, which is difficult to obtain through field observation by conventional device. Algorithms which automatically detect locations, shapes, and magnitudes of rip currents were developed using time averaged X-band radar sea clutter images. X-band sea clutter images are transformed through 3D FFT into 2D wave number spectrum and frequency spectrum. Rip current velocities were estimated using differences in wave-number spectra and wave frequency spectra due to Doppler shift. The algorithm was verified by drift experiments. At Haeundae beach, the radar system exactly located the rip currents and found to be sustained for 1-2 days at fixed locations.

• Magazine of the Korean Society of Agricultural Engineers
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• v.44 no.2
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• pp.81-98
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• 2002

The condition of initial movement far the river bank protection using rip rap is a limit condition beyond which the lining is progressively destroyed as the separate elements are removed by the flow. In the case of the river bank protection using gabion mattress, however, after the initial movement the containment offered by the mesh remains. A new situation of equilibrium with a deformed river bank protection using gabion mattress is obtained, allowing it to withstand more severe conditions without compromising the resistance and without further deformation. Shield's coefficient for the river bank protection using gabion mattress is twice the value of that fur river bank protection using rip rap. This means that with the same hydraulic behavior conditions, the average dimension of the rocks to be used in the river bank protection using gabion mattress is half that of river bank protection using rip rap rock. When the same size rocks are used the allowable velocity for the river bank protection using gabion mattress is more than twice, even as much as 3 or 4 times that for river bank protection using rip rap.

• Research in Community and Public Health Nursing
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• v.30 no.4
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• pp.460-470
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• 2019

Purpose: This study was to identify the factors influencing the practice of respiratory infection prevention (RIP) for the elderly residing in rural areas. Methods: The data were collected from 188 residents aged 65 years or older residing in the jurisdictions of four public health clinics in rural areas of C city, using a structured questionnaire for the period from December 1, 2018 to February 28, 2019. The collected data were analyzed with independent t-test, one-way ANOVA, Pearson's correlation coefficients, and hierarchical multiple regression analysis using SPSS/WIN 25.0 program. Results: The score on knowledge of RIP was 8.82±1.36, while it was 4.53±0.47 for attitude, 3.78±0.66 for practice, and 3.78±0.69 for social capital. It was observed that the factors influencing the practice of RIP were attitude of RIP (β=.38, p<.001), social capital (β=.29, p<.001), family type (β=-.19, p=.002), and subjective health status (β=.15, p=.035), while the explanatory power of the model was 47%. Conclusion: Consequently, to enhance the practice of RIP for the elderly residing in rural areas, it is necessary to develop programs considering social and environmental characteristics of rural areas based on their attitude of RIP, social capital, family type and subjective health status.

• Journal of Broadcast Engineering
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• v.17 no.6
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• pp.954-963
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• 2012

As a greedy algorithm reconstructing the sparse signal from underdetermined system, orthogonal matching pursuit (OMP) algorithm has received much attention. In this paper, we multiple candidate matching pursuit (MuCaMP), which builds up candidate support set in every iteration and uses the minimum residual at last iteration. Using the restricted isometry property (RIP), we derive the sufficient condition for MuCaMP to recover the sparse signal exactly. The MuCaMP guarantees to reconstruct the K-sparse signal when the sensing matrix satisfies the RIP constant ${\delta}_{N+K}<\frac{\sqrt{N}}{\sqrt{K}+3\sqrt{N}}$. In addition, we show a recovery performance both noiseless and noisy measurements.