• BMB Reports
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• v.45 no.6
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• pp.331-336
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• 2012

The retinoid-related orphan nuclear receptor gamma ($ROR{\gamma}$) plays critical roles in regulation of development, immunity and metabolism. As transcription factor usually forms a protein complex to function, thus capturing and dissecting of the $ROR{\gamma}$ protein complex will be helpful for exploring the mechanisms underlying those functions. After construction of the recombinant tandem affinity purification (TAP) plasmid, pMSCVpuro $ROR{\gamma}$-CTAP(SG), the nuclear localization of $ROR{\gamma}$-CTAP(SG) fusion protein was verified. Following isolation of $ROR{\gamma}$ protein complex by TAP strategy, seven candidate interacting proteins were identified. Finally, the heat shock protein 90 (HSP90) and receptor-interacting protein 140 (RIP140) were confirmed to interplay with $ROR{\gamma}$ by co-immunoprecipitation. Interference of HSP90 or/and RIP140 genes resulted in dramatically decreased expression of CYP2C8 gene, the $ROR{\gamma}$ target gene. Data from this study demonstrate that HSP90 and RIP140 proteins interact with $ROR{\gamma}$ protein in a complex format and function as co-activators in the $ROR{\gamma}$-mediated regulatory processes of HepG2 cells.

• Proceedings of the Korea Concrete Institute Conference
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• 2005.11a
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• pp.137-140
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• 2005

This paper focuses on the premature failure of RC beams bonded with FRP. A number of failure modes for RC beams bonded with FRP have been observed in numerous experimental studies during past decade. Particularly, Rip-off failure and Debonding failure were majority failure modes in RC beams bonded with FRP. Rip-off failure occurred at the plate end due to high interfacial shear and normal stresses however Debonding failure was caused by the yielding of reinforcing bar and the increasing of shear deformation in shear span. On the basis of premature failure mechanism in RC beams bonded with FRP, Basic strengthening length and Premature failure criteria were derived

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.04a
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• pp.140-141
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• 2005

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.30 no.3
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• pp.150-160
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• 1994

Drift gillnet fishery for neon flying squid in the North pacific was one of the major pelagic fisheries of Korea until 1992, its annual catch was 79, 000M/T as average during 1988-1992, but moratoriumed since 1993 according to the decision of UN. Therefore, for the developing of the new fishing gear for the squid, the seven types of rip hook by automatic squid jigging machine were experimented by the korean research vessel Pusan 851 (G/T 1.126, 2.600 PS) in the North Pacific (38 $^{\circ}$30'-43 $^{\circ}$N, 152 $^{\circ}$E-178 $^{\circ}$W) from July 6. 1993 to August 31. 1993. The investigation on catch rate, dropout rate, and catch condition of the rip hooks related to the fishing lamp power for aggregating the squid were carried out during the period. The results obtained are as follows: The composition of catch by automatic squid jigging machine was 83.9% for neon flying squid. 15.5% for boreopacific gonate squid. 0.6% for boreal clubhook squid, and 0.01% for luminous flying squid. The catch rate of neon flying squid was 94.6% in 13.6-18.3$^{\circ}C$ of surface water temperature and 5.4% in others. The higher catch rate of neon flying squid was made in the range 13.6-18.3$^{\circ}C$ of temperature at the surface and about 1$0^{\circ}C$ of temperature at the 100m layer. The CPUE of neon flying squid in the 13.6-18.3$^{\circ}C$ of surface water temperature was ranged 0.8-11.8kg (8.7kg as average). The mantle length and body weight of neon flying squid caught in the experiment were ranged 18.3-51.3 cm, 140-3, 980g and mean mantle length and mean body weight were 29.4cm, 972g respectively. The catch rate of neon flying squid was the highest at dawn with a value of 25.0% of the total catch. The body weight of neon flying squid caught by the D type hooks was 1.7 times more than that of the A type hooks. The dropout rate of neon flying squid caught by the seven types hooks was 7.9-57.5% (19.0% as average), and dropout rate of the D type hooks was 7.9% with 2.7 times decrease than that of the A type hooks. The catch efficiency of small sized neon flying squid in case of using on-off switch method on fishing lamp in 15 minutes intervals was 2.6 times higher than that of the on-switch method with same fishing lamp power.

• Journal of Ocean Engineering and Technology
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• v.31 no.2
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• pp.130-140
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• 2017

Typically, a submerged breakwater is one of the good scene-friendly coastal structures used to reduce wave energy and coastal erosion. However, sometimes, a submerged breakwater also has a negative aspect in that a strong rip current occurring around an open inlet due to a difference in mean water levels on the front and rear sides of the structure leads to scouring. Such scouring has a bad effect on its stability. In order to eliminate this kind of demerit, this study investigated four new types of submerged breakwaters with drainage channels. First, hydraulic experiments were performed the typical and new structures. Then, the wave height and mean water level distributions around the structures were examined using the experimental results. Finally, it was revealed that the new type of submerged breakwater could efficiently reduce the mean water level on its rear side. In particular, in the case of new-type submerged breakwater 2, an average reduction efficiency of 71.2% for the difference between the mean water levels at the front and rear sides was shown in comparison with the typical one.

• Journal of Life Science
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• v.17 no.12
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• pp.1622-1626
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• 2007

This study was conducted to identify useful single nucleotide polymorphisms (SNPs) and determine their association with economically important traits in pig population. Four candidate gene analyses have identified important chromosomal regions and major genes associcated whit economic traits of the pig. For application of the chromosomal information to the pig industry using DNA technology, SNP markers were developed by comparative re-sequencing of polymerase chain reaction (PCR) products of 4 candidate genes (CSF2, IL4, MYOD, RIP140). PCR restriction fragment length polymorphism (PCR-RFLP) assays were developed for these 4 SNPs and used to genotype Berkshire pig populations in Korea.