• Fisheries and Aquatic Sciences
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• v.2 no.2
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• pp.176-181
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• 1999

The variations of gene expression and pituitary contents of GTH subunits during the ovarian development of masu salmon, Oncorhynchus masou, were investigated. The pituitary GTHs contents was measured by radioimmunoassays (RIAs) using purified GTH subunits and their antibodies. Pituitary contents of GTH $I\beta$ gradually increased from April through August, and reached the maximum in October. On the other hand, pituitary contents of GTH $II\beta$ remained low until August, but they rapidly increased in October. Total RNAs were prepared from pooled pituitaries and the GTH subunits mRNA in pituitaries was quantified by Northern blot hybridization using masu salmon cDNA probes for each GTH subunit. GTH $I\beta$ mRNA level increased with the progression of ovarian maturity. However, GTH $II\beta$ mRNA was detected only at a more advanced stage, and were extremly high at ovulation. A high levels for GTH a mRNA was detected only at ovulation stage. The synchronous increase in pituitary contents and mRNA levels suggested that ovarian maturity in masu salmon was regulated by both GTH I and GTH II.

• Fisheries and Aquatic Sciences
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• v.3 no.2
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• pp.135-142
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• 2000

In order to clarify the role of gonadal sex steroids in the synthesis of gonadotropin (GTH) subunits in immature rainbow trout, we examined in vitro and in vivo effects of testosterone (T) on the pituitary mRNA levels of GTH I $\beta$, GTH II$\beta$ and a subunits by Northern blot analysis and on the pituitary content levels of GTH I$\beta$ and GTH II$\beta$by radioimmunoassay (RIA). The mRNA levels of the a subunit in T-treated fish were not changed more dramatically than those in control fish both in vivo and in vitro. Interestingly, the mRNA levels of GTH I$\beta$ in T-treated fish were shown to be slightly lower than those in the control fish under these experimental conditions, but no differences were observed in pituitary GTH I$\beta$ contents. In contrast, the mRNA levels and pituitary contents of GTH II$\beta$ subunit were strongly increased by T both in vivo and in vitro. These results demonstrate that the expressions of GTH I$\beta$ and II$\beta$ subunit genes in immatue rainbow trout pituitary are subjected to differential regulation by T.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.19 no.4
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• pp.469-475
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• 2010

The objective of this study is to integrate reliability analysis into shape optimization problem using the evolutionary structural optimization (ESO) in the application example. Reliability-based shape optimization is formulated as volume minimization problem with probabilistic stress constraint under minimization max. von Mises stress and allow stress. Young's modulus, external load and thickness are considered as uncertain variables. In order to compute reliability index, four methods, i.e., reliability index approach (RIA), performance measure approach (PMA), single-loop singlevector (SLSV) and adaptive-loop (ADL), are used. Reliability-based shape optimization design process is conducted to obtain optimal shape satisfying max. von Mises stress and reliability index constraints with the above four methods, and then each result is compared with respect to numerical stability and computing time.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.351-353
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• 2003

We present a retrieval scheme for the remote sensing of evapotranspiration (ET) over rice paddy. To perform the retrieval, high-resolution airborne imagery of multi-spectral visible and thermal infrared data, and ground-based meteorological measurements are utilized. Our ET retrieval scheme is based on the basic principal of surface energy budget, which is a result of balance in longwave and shortwave radiation, latent heat, sensible heat, and energy flux into the ground. To partition the latent and sensible heat fluxes of interest from the energy balance equation, three basic parameters are of most concern, including albedo, surface temperature, and normalized difference vegetation index (NDVI). The NDVI and albedo can be easily derived from the visible and near infrared spectral data, while the surface tem-perature can be determined through the analysis of the infrared data with the Stefan Boltzmann law. From the airborne imagery taken on 28 April 2003, we observe very good dry and wet pixels that can be easily corre-sponded to the radiation and evaporation controlled crite-ria, respectively, and, hence, for the further use in defin-ing the evaporative fraction needed to partition sensible and latent heat fluxes from the net energy flux. The de-rived ET is compared with the in situ measurements.

• Asian Journal of Innovation and Policy
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• v.9 no.3
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• pp.281-307
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• 2020

A variety of approaches are being applied to improve the existing ex-ante evaluation by expert panels in publicly funded R&D. While the objective evaluation criteria are constantly being improved to screen and select the superior projects, alternative approaches such as random prioritization and logical modeling are also underway to overcome the conservative bias of reviewers and to secure disruptive innovation. This study intends to find critical implications for ex-ante evaluation of public R&D system from the comparison of Indonesia and Korea. For the comparative analysis, literature review and expert in-depth interviews are conducted on the national R&D system and the selection evaluation process. In Korea, the selection criteria of projects are legally promulgated for establishing an objective evaluation system, and at the program level, the major considerations in the planning process are specified by Presidential Decree. On the other hand, while Indonesia conducts R&D in 47 strategic fields largely by public research institutes (PRI) based on the non-competitive government contributions. This study draws out implications of institutionalizing the planning process at the level of program, and of increasing the ratio of contract-based competitive funding at the level of project in the national R&D portfolio.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2022.10a
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• pp.620-622
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• 2022

When an optical fiber is exposed to radiation, a color center is formed in the core, which lowers the optical transmittance of the optical fiber. This is called the radiation-induced attenuation(RIA), and research on optical fibers having improved radiation resistance by changing materials and structures is being actively conducted. This is because radiation-resistant optical fibers have the advantage that they can be used in telecommunication and optical applications even in extreme environments such as space and nuclear power plants. In this paper, the effect of gamma irradiation of commercial radiation-resistant optical fibers was analyzed.

• Architectural research
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• v.25 no.4
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• pp.73-84
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• 2023

Indoor thermal conditions greatly affect the health and comfort of humans who occupy the space in it. The purpose of this research is to analyze the influence of water and vegetation elements as a microclimate modifier in buildings to obtain thermal comfort through the study of thermal environment models. This research covers two objects, namely public buildings and housing in Makassar City, South Sulawesi Prov-ince - Indonesia. Quantitative methods through field surveys and measurements based on thermal and personal variables. Data analysis based on ASHRAE 55 2020 standard. The data was processed with a parametric statistical approach and then simulated with the Computational Fluid Dynamics (CFD) simulation method to find a thermal prediction model. The model was made by increasing the ventilation area by 2.0 m2, adding 10% vegetation with shade plant characteristics, moving water features in the form of fountains and increasing the pool area by 15% to obtain PMV + 0.23, PPD + 8%, TSV-1 - +0, Ta_25.7℃, and relative humidity 63.5 - 66%. The evaluation shows that the operating temperature can analyze the visitor's comfort temperature range of >80% and comply with the ASHRAE 55-2020 standard. It is concluded that water elements and indoor vegetation can be microclimate modifiers in buildings to create desired comfort conditions and adaptive con-trols in buildings such as the arrangement of water elements and vegetation and ventilation systems to provide passive cooling effects in buildings.

• Clinical and Experimental Vaccine Research
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• v.12 no.1
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• pp.13-24
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• 2023

This systematic and meta-analysis aims to evaluate humoral and cellular responses to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine among kidney transplant recipients (KTRs). We conducted a systematic literature search across databases to evaluate seroconversion and cellular response rates in KTRs receiving SARS-CoV-2 vaccines. We extracted studies that assessed seroconversion rates described as the presence of antibody de novo positivity in KTRs following SARS-CoV-2 vaccination published up to January 23rd, 2022. We also performed meta-regression based on immunosuppression therapy used. A total of 44 studies involving 5,892 KTRs were included in this meta-analysis. The overall seroconversion rate following complete dose of vaccines was 39.2% (95% confidence interval [CI], 33.3%-45.3%) and cellular response rate was 41.6% (95% CI, 30.0%-53.6%). Meta-regression revealed that low antibody response rate was significantly associated with the high prevalence of mycophenolate mofetil/mycophenolic acid (p=0.04), belatacept (p=0.02), and antiCD25 induction therapy uses (p=0.04). Conversely, tacrolimus use was associated with higher antibody response (p=0.01). This meta-analysis suggests that postvaccination seroconversion and cellular response rates in KTRs are still low. And seroconversion rate was correlated with the type of immunosuppressive agent and induction therapy used. Additional doses of the SARS-CoV-2 vaccine for this population using a different type of vaccine are considered.

• The korean journal of orthodontics
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• v.23 no.3 s.42
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• pp.415-425
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• 1993

Fluoride is one of the most potent stimulators of bone formation in vivo. But its direct effects on osteoblast is not yet clear This study was to investigate the effects of Sodium fluoride on alkaline phosphatase(ALP) activity, cAMP formation responsive to parathormone(PTH) and type I $\alpha$ 2 collagen ribonucleic acid (mRNA) level in Murin osteoblast-like (MC3T3-E1) cells. The cells were cultured in $\alpha-Minimal$ essential medium $(\alpha-MEM)$ supplemente with $10\%$ fetal bovine serum (FBS) and then changed to $0.1\%$ FBS with various concentration of Sodium fluoride. The ALP activity was assayed by the method of Lowry with disodium phenyl phosphated as substrate. cAMP formation was measured by Radioimmuno Assay(RIA). Type I $\alpha$ 2 collagen ribonucleic acid(mRNA) expression was studied by Nothern blot analysis. The results were as follows: 1. cAMP level was increased by PTH in MC3T3-E1 cells. 2. Sodium fluoride showed the tendency of inhibitory effects on cAMP responsiveness to PTH in MC3T3-E1 cells. 3. Sodium fluoride increased ALP activity at cocentration of $2{\mu}M,\;4{\mu}M,\;and\;10{\mu}M$ significantly different from control at the 0.001 level. ALP activity revealed maximum value at $10{\mu}M$ in this study. 4. Nothern blot analysis of Sodium fluoride treated cells, using Type I $\alpha$ 2 collagen prove, revealed significant increase at $10{\mu}M$ in MC3T3-E1 cells.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.6
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• pp.506-512
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• 1991

Monoclonal antibodies (mAb) to indole-3-acetic acid (IAA) were produced and characterized. Spleen cells from mouse immunized with IAA coupled to bovine serum albumin were fused with SP2/0-Ag14 myeloma cells. Three clones secreted specific antibodies to IAA were established to hybridoma cell lines and designated WLI-G1, WLI-G3 and WLI-Ell. The antibodies produced were classified into IgG, types and revealed the high degree of specificity by cross-reaction in the IAA derivatives and its analogues. In the IAA-ELISA with mAb, the measuring range of the assay was 1-500 p mol, and Ka and binding capacity calculated from Scatchard plot were 6.7 X 10$^{-10}$ L/M and 6 x 10$^{-10}$ L/M respectively. The ELISA with mAb can be used to quantitate IAA directly in crude plant eatract. The results showed that the immunoassay was easy and sensitive method to perform and applicate for quantitative analysis of IAA in plant.