• Korean Journal of Microbiology
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• v.54 no.4
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• pp.343-353
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• 2018

Our previous research demonstrated the essential role of the xenB gene in stress response to RDX by using Pseudomonas sp. HK-6 xenB knockout. We have extended this work to examine the cellular responses and altered proteomic profiles of the HK-6 xenA knockout mutant under RDX stress. The xenA mutant degraded RDX about 2-fold more slowly and its growth and survival rates were several-fold lower than the wild-type HK-6 strain. SEM revealed more severe morphological damages on the surface of the xenA mutant cells under RDX stress. The wild-type cells expressed proportionally-increased two stress shock proteins, DnaK and GroEL from the initial incubation time point or the relatively low RDX concentrations, but slightly less expressed at prolonged incubation period or higher RDX. However the xenA mutant did not produced DnaK and GroEL as RDX concentrations were gradually increased. The wild-type cells well maintained transcription levels of dnaA and groEL under increased RDX stress while those in the xenA mutant were decreased and eventually disappeared. The altered proteome profiles of xenA mutant cells under RDX stress also observed so that the 27 down-regulated plus the 3 up-regulated expression proteins were detected in 2-DE PAGE. These all results indicated that the intact xenA gene is necessary for maintaining cell integrity under the xenobiotic stress as well as performing an efficient RDX degradation process.

• Microbiology and Biotechnology Letters
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• v.31 no.1
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• pp.75-82
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• 2003

The cellular responses of RDX-degrading bacterium, Pseudomonas sp. HK-6 to explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) were examined. Strain HK-6 grown at different RDX concentrations was found to demonstrate the survival rate in proportional to the rate of the stress shock proteins produced in this bacterium. Analysis of total cellular fatty acid acids showed that lipids 10:0 iso and 14:1 $\omega$5c/$\omega$5t increased approx three times in strain HK-6 grown on RDX media than TSA media. SDS-PAGE and Western blot using anti-DnaK and GroEL revealed that several stress shock proteins including 70 kDa DnaK and 60 kDa CroEL were newly synthesized in strain HK-6 exposed to different RDX concentrations in exponentially growing cultures. 2-D PAGE of soluble protein fractions from the culture of HK-6 exposed to RDX demonstrated that approximately 300 spots were observed on the silver stained gel ranging from pH 3 to pH 10. As a result, 10 spots were significantly induced and expressed in response to RDX. Scanning electron microscopy fur the cells treated with 0.135 mM RDX for 12 hrs showed the presence of perforations and irregular rod shapes with wrinkled surfaces.

• Journal of the Korea Institute of Military Science and Technology
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• v.3 no.2
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• pp.219-230
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• 2000

Plastic bonded explosive(PBX) is mainly composed of the nitramine explosives, RDX, HMX, and polymer binders. When the adhesion between nitramine crystals and binder is not particularly strong and can be failed under stress, dewetting occurs rather suddenly and this leads to a significant drop in tensile strength of explosives. Mechnical property of plastic bonded explosive depends on the surface characteristics of filler and binder. In order to design for better adhesion, an understanding of the surface properties of explosive and binder is essential. In this study, 2 kinds of RDX and 4 kinds of ethylene vinyl acetate copolymers are selected, since they are widely used in many plastic bonded explosives. The technical objective of this investigation is to calculate for the surface free energy of RDX and EVA using theory of Fowkes, van Oss, Neumann approaches and Kaelble equation and to predict the interaction between filler and binder from their surface free energies.

• Journal of Soil and Groundwater Environment
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• v.20 no.6
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• pp.73-84
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• 2015

Nine plant species were selected through vegetation survey at three military shooting ranges at northern Gyeonggi Province. Plants were germinated in normal soil and three seedlings were transplanted to a bottom sealed pot containing sandy loam soils contaminated with either RDX (291 mg/kg) or TNT (207 mg/kg). Planted, blank (without plant), and control (without explosive compound) pots were grown in triplicate at a green house for 134 days. During cultivation, transplanted plants exhibited chlorosis and necrosis in flower and leaf by explosive toxicity and stress. Only three plants, Wild soybean, Amur silver grass, Reed canary grass, survived in TNT treated pot, while seven plant species except for field penny cress and jimson weed, thrived in RDX treated pot. Appreciable amount of TNT (61.6~241.2 mg/g-D.W.) was detected only in plant roots. Up to 763.3 mg/g-D.W. along with 4-amino-2,6-dinitrotoluene, an intermediate of TNT, accumulated in the root of wild soybean. In addition, azoxy compounds, abiotic intermediates of TNT, were detected in TNT treated soils. RDX absorbed average 1,839.95 mg/kg in shoot and 204.83 mg/kg in root. Most of TNT in plant was accumulated in underground part whereas RDX was localized in aerial part. Material balance calculation showed that more than 95% of the initial TNT was removed in the planted pots whereas only 60% was removed in the blank pot. The amount of RDX removed from soil was in the order of Amur Silver Grass (51%) > Chickweed (43%) > Evening primrose (38%). Based on the results of pot cultures, Amur silver grass and Reed canary grass are selected as tolerant remedial plants for explosive toxicity.

• Journal of Welding and Joining
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• v.18 no.4
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• pp.38-47
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• 2000

Characteristics of the interface between tube and tube sheet which were formed by explosive expansion and roll expansion, have been studied in the research. The results are as follows: Optimum amounts of explosives for the expansion of Alloy 600 (19.05mm and 15.88mm) were found to be RDX 3.5-8.5g/m. Because explosive expansion caused les strain hardening and increased bounding strength, characteristics of the explosively expanded were better than those of mechanically expanded. As the transition region of the explosive expansion is inactive, the resistance to the stress corrosion cracking increases by 30∼40% compared to the roll and hydraulic expansion.

• Polymer(Korea)
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• v.38 no.2
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• pp.225-231
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• 2014

The rheological properties of highly concentrated polymer bonded explosive simulant were studied by using poly(ethylene-co-vinyl acetate) with 30 and 60% vinyl acetate (VA) content as a binder, respectively. Calcium carbonate and Dechlorane, whose physical properties are similar to resarch department explosive (RDX)'s, were used as fillers. The suspensions were mixed in a batch melt mixer and it was possible to fill 75 v% at maximum. From dynamic mechanical analysis, Dechlorane showed higher interaction with binder resins than that with calcium carbonate fillers. The effects of microstructural change on the rheological properties of the suspensions were investigated by a plate-plate rheometer with constant shear rate and constant shear stress modes, respectively. The theoretical maximum packing fraction of EVA31/Dechlorane suspension obtained from Krieger-Dougherty equation was 70 v% and it was thought that 2000 Pa was proper shear stress condition for this melt processing.

• Explosives and Blasting
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• v.17 no.4
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• pp.32-50
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• 1999

This study deals with interface charactristics of tube and tubesheet of the nuclear steam generator by the explosive expansion in order to take advantage of optimum expansion ratio, pull-out strength and leakage tightness and improvement of the resisitance on the stress corrosion cracking for low residual stress. The paper also show the relationship between roll, hydraulic and explosive expansion. The results obtain are as follows (1) Because of the explosive bonding is to use the high speed pressure and energy by the explosive, workability is good, bonding region is homogenous (2) Expansion ratio is 2.7%, Pull-out strength 850kg, Leakage strength $500kg/cm^2$. Clearance gap is 10~30mm in case of explosive expansion and interface structure of the tube and tubesheet is optimum condition. (3) As the transition region of the explosive expansion is inactive, the resistance of the stress corrosion cracking is increases 30~40% compare to the roll and hydraulic expansion.

• International Journal of Aeronautical and Space Sciences
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• v.16 no.1
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• pp.50-63
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• 2015

Explosive bolts are one of pyrotechnic release devices, which are highly reliable and efficient for a built-in release. Among them, ridge-cut explosive bolts which utilize shock wave generated by detonation to separate bolt body produce minimal fragments, little swelling and clean breaks. In this study, separation phenomena of ridge-cut explosive bolts or ridge-cut mechanism are computationally analyzed using Hydrocodes. To analyze separation mechanism of ridge-cut explosive bolts, fluid-structure interactions with complex material modeling are essential. For modeling of high explosives (RDX and PETN), Euler elements with Jones-Wilkins-Lee E.O.S. are utilized. For Lagrange elements of bolt body structures, shock E.O.S., Johnson-Cook strength model, and principal stress failure criteria are used. From the computational analysis of the author's explosive bolt model, computational analysis framework is verified and perfected with tuned failure criteria. Practical design improvements are also suggested based on a parametric study. Some design parameters, such as explosive weights, ridge angle, and ridge position, are chosen that might affect the separation reliability; and analysis is carried out for several designs. The results of this study provide useful information to avoid unnecessary separation experiments related with design parameters.

• Explosives and Blasting
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• v.17 no.4
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• pp.18-31
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• 1999

The explosive forming has been used for many year to expand tubes into tubesheets. this process has demonstrated ability to direct carefully the energy of an explosive to expand tubes into tubesheet holes without damaging the tubesheet and without causing the excessive cold work at the tube I.D. that is normally associated with mechanical expansion. The success of explosive tube expansion provided the background for the development of the explosive tube plug. The main results are as follows : (1) The optimum explosives and explosive qualities are PETN, RDX, HMS and about 18~31gr/ft of explosive plugging in nuclear steam generator. (2) Explosive plugging's thickness is 0.9~1.8mm. If groove of 0.4 mm formed in plug outside, For the hydraulic leakage is go up, explosive plugging of formed groove are applicate tube and tubrplate. (3) Sheath is designed on the polyethylene of low density, In thermal impact test of the $430^\circ{C}$, hydraulic leakage is $300kg/cm^2$. (4) About 10~60mm oxide inclusions are existed on the space of explosive plug and tube protect to the leakage.