• Title/Summary/Keyword: RDA 2020

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Talaromyces halophytorum sp. nov. Isolated from Roots of Limonium tetragonum in Korea

  • You, Young-Hyun;Aktaruzzaman, Md.;Heo, Inbeom;Park, Jong Myong;Hong, Ji Won;Hong, Seung-Beom
    • Mycobiology
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    • v.48 no.2
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    • pp.133-138
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    • 2020
  • Talaromyces halophytorum sp. nov. was isolated from the roots of halophyte Limonium tetragonum collected from Seocheon-gun, Korea in November 2015. It showed a slow growth on yeast extract sucrose agar at 25 ℃, no growth at 4 ℃ or 37 ℃ and produced smooth-walled and globose to sub-globose conidia. T. halophytorum is phylogenetically distinct from the other reported Talaromyces species of section Trachyspermi based on multi-locus sequence typing results using partial fragments of β-tubulin, calmodulin, ITS, and RNA polymerase II genes.

Application of biodegradable metal based drug delivery carrier on Foot and Mouth Disease vaccination in pigs (돼지에서 생체 분해 금속성 전달체를 활용한 구제역 백신의 적용 연구)

  • Cho, Ara;Oh, Sang-Ik;Choe, Changyong;Jung, Young-Hun;Do, Yoon Jung;Kim, Suhee;Choo, Hyun Wook;Gu, Jong Su;Chung, Woo-Jin;Cho, Sung Youn;Yoo, Jae Gyu
    • Korean Journal of Veterinary Service
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    • v.43 no.3
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    • pp.129-137
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    • 2020
  • In this study, we applied biodegradable drug delivery carries (BDDC) for food-and-mouth (FMD) vaccination. After FMD vaccination using BDDC, we estimated the percentage inhibition (PI) of antibody, decomposed patterns, and histopathologic features of BDDC. PI of antibody was higher than 50 at two weeks after injection and sustained positive PI until 10 weeks after injection. BBDC injection group showed significantly an increased pattern of blood monocyte at two and three weeks after injection. According to the Micro CT, micro-cracks were observed at two weeks after injection and the morphology of BDDC was lost at four weeks after injection. For histopathological examination, acute inflammation with neutrophil infiltration and micro-metallic residues were observed around BDDC until four weeks after injection and inflammatory responses gradually decreased at 10 weeks. Based on our experiment, BDDC is considered as an alternative way to vaccine injection for veterinary applications. Our study can be used as basic data for the drug delivery system using biodegradable metals in the future.

Manufacturing Conditions for Rice Porridge with Optimum Properties after Microwave Range Reheating (마이크로웨이브 레인지 재가열 후 최적 특성을 갖는 쌀죽 제조조건)

  • Park, Hye-Young;Kim, Hyun-Joo;Sim, Eun-Yeong;Kwak, Jieun;Chun, Areum;Jo, Youngje;Woo, Koan Sik;Kim, Mi Jung
    • The Korean Journal of Food And Nutrition
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    • v.33 no.4
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    • pp.440-446
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    • 2020
  • The purpose of this study was to derive the conditions for manufacturing rice porridge with optimum properties after reheating. The characteristics of rice porridge according to the soaking time, water addition rate, heating temperature, heating time, and cooling conditions were compared using the 'Samkwang' cultivar. In Step I, as the heating temperature increased, the weight change decreased and the viscosity increased, and the temperature known as the main factor of the gelatinization also appeared to affect the viscosity increase. In Step II, the viscosity and the texture properties was not significantly different as the soaking time was reduced, and 10 minutes was suitable because of due to the shortening effect of the total process time. In Step III, the residual heat was lowered by cooling after the rice porridge production, so the viscosity could be greatly reduced. Also, it was confirmed that the water addition rate of 900% and the heating temperature of 15 minutes were optimal manufacturing conditions. The next study will investigate the porridge processability of rice cultivars using these results.

Physicochemical Properties and Anti-inflammatory Effects of Astragalus membranaceus (Fisch.) Bunge Fermented by Aspergillus awamori (Aspergillus awamori로 발효한 황기 열수 추출물의 이화학적 특성과 항염증 효과)

  • Lee, Eun Jung;Lee, Da Bin;Song, Bit Na;Park, Bo Ram;Lee, Sung Hyen;Choi, Ji Ho;Park, Shin Young
    • Korean Journal of Medicinal Crop Science
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    • v.28 no.5
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    • pp.347-353
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    • 2020
  • Background: Fermentation of medicinal plants increases their absorption rate and bioavailability in the body. Astragalus membranaceus has been used as a raw material, but research in its use as a food ingredient is lacking. Therefore, the purpose of this study was to identify the physicochemical characteristics and anti-inflammatory effect of fermented Astragalus membranaceus. Methods and Results: Astragalus roots were fermented using Aspergillus awamori for 4 days and their extracts prepared using hot water. The pH, total acidity (%), and reducing sugar (%) of the extracts were then investigated. The pH and total acidity decreased during fermentation. After fermentation, the pH and total acidity decreased, whereas the reducing sugar level increased. The active ingredients in fermented Astragalus were calycosin-7-O-ßd-glucoside, ononin, calycosin and formononetin. The calycosin contents was highest in the hot-water extracted samples fermented for 4 days. The other components were similar to those in control. Nitric oxide level was lower in the hot-water extracted samples fermented for 4 days than in lipopolysaccharide control group. The sample fermented for 4 days was confirmed to inhibit the production of tumor necrosis factor-α and interleukin-1β. Conclusions: Our results showed the physicochemical properties and anti-inflammatory effects of A. membranaceus after fermentation using Aspergillus awamori. These results indicated that fermented Astragalus membranaceus can be used as a functional food.

A Study on Establishment of Similar Expousre Groups(SEGs) for Chemical and Biological Risk Factors in Farm Work (농작업시 발생하는 화학적 및 생물학적 위험요인에 대한 유사노출작업군 설정 연구)

  • Lee, Minji;Sin, Sojung;Kim, Hyocher;Heo, Jinyoung;Ahn, Minji;Kim, Kyungran;Kim, Kyungsu
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.30 no.3
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    • pp.292-298
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    • 2020
  • Objectives: The aim of this research is to establish Similar Exposure Groups (SEGs) for chemical and biological risk factors that occur in farm work involving 24 tasks among 15 crops. Methods: To categorize SEGs, work type, work environment, and similar tasks for each crop were considered. After confirming the chemical risk factors (pesticides, inorganic dust-total dust and PM10, ammonia, and hydrogen sulfide) and biological factors (organic dust-total dust and PM10, and endotoxins) that occur in the crops and tasks, similar crops and tasks were selected as SEGs. Results: Among chemical risk factors, pesticides was selected for the SEGs, which was categorized by open field, greenhouse, fruit, and specialty crops. For inorganic dust, open field (plowing harrowing, seedling, planting, harvest, and sorting and packing) and specialty crops (plowing harrowing, seedling, planting, and harvest) were selected as SEGs. For ammonia and hydrogen sulfide, livestock (preparation of farm, management of nursery bed, feeding, shipment and manure treatment) were selected as SEGs. For biological risk factors such as organic dust (total dust, PM10) and endotoxins, open field (manure application), greenhouse (plowing harrowing, planting, manure application, and harvest), fruit (manure application), specialty crops (manure application, making furrows, mixing mushroom media, harvest, and sorting and packing), and livestock (preparation of farm, maintaining poultry litter, feeding, shipment and manure treatment) were selected as SEGs. Conclusions: To establish similar exposure groups in agriculture, it is important that the characteristics of each hazard factor are categorized by identifying risk factors occurring by tasks.

Antioxidant Activity of Chinese Cabbage (Brassica rapa L. ssp. pekinensis) using Different Genotypes (배추 유전자원의 항산화 효과)

  • Hwang, Byung Soon;Kim, Ji Yeong;Kwon, Su Hyun;Kim, Gi-Chang;Kang, Hae Ju;Rhee, Ju Hee;Hwang, In Guk
    • The Korean Journal of Food And Nutrition
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    • v.33 no.5
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    • pp.532-543
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    • 2020
  • This experiment was conducted to assess the high antioxidant activity varieties of Chinese cabbage (Brassica rapa L. ssp. pekinensis) from the 55 accessions. The antioxidant activity of Chinese cabbage were determined by the TPC, TFC, DPPH, ABTS, and chlorophyll, carotenoid contents. The TPC and TFC showed a range of 1.21~4.61 mg GAE/g DW, 0.18~3.09 mg CE/g DW. The DPPH and ABTS assay were in the range of 0.65~4.36 and 1.42~6.91 mg ascorbic acid equivalent (ASCE)/g DW, respectively. The UPLC analysis was performed quantitatively to identify chlorophyll and carotenoid in the Chinese cabbage extract. The levels of the total chlorophyll and total carotenoid were 86.60~1,235.91, and 75.86~490.11 ㎍/g, respectively. The comprehensive differences in the total and individual chlorophyll contents have also been observed among different varieties. These results will be valuable as basic data for the standardization of Chinese cabbage.

Comparison of Tastes-Related Components and Eating Quality between Hanwoo Steer and Cow Longissimus thoracis Muscles

  • Cho, Soohyun;Seol, Kukhwan;Kang, Sunmoon;Kim, Yunseok;Seo, Hyunwoo;Lee, Wangyeol;Kim, Jinhyoung;Ba, Hoa Van
    • Food Science of Animal Resources
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    • v.40 no.6
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    • pp.908-923
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    • 2020
  • The objective of this study was to compare tastes-related components and eating quality properties between Hanwoo steer and cow meats. Longissimus thoracis (LT) muscles with same quality grade (1+ grade) collected from left sides of the steer and cow carcasses were used for analyses of technological quality traits, free amino acids (FAAs), metabolites, nucleotides, fatty acids and sensory attributes. there were no differences occurring in the chemical composition (fat, protein, moisture and collagen) and technological quality traits (cooking loss, water holding capacity, shear force and color) between the two beef types (p>0.05). The cow meat exhibited significantly higher amounts of some FAAs associated with umami (e.g., glutamic acid and lysine), sweetness (e.g., proline and glutamic acid) and saltiness (e.g., histidine and glutamic acid) compared to the steer meat (p<0.05). Regarding the nucleotides, no differences occurred in all the identified nucleotides between the two beef types (p>0.05). A total of 27 metabolites were identified, however, only some compounds (e.g., acetate, creatine, creatinine, glucose and inosine, etc.) showed their significantly higher amounts in the cow meat compared with those in the steer meat (p<0.05). In terms of sensory aspects, the panelists found no differences in scores of all the eating quality traits between the two sex types of beef (p>0.05). Overall, excepts some variations in tastes-active compounds, there were no differences in the quality characteristics in general and eating quality in particular between the cow and steer meats when they were in the same quality grade.

Effect of Timing of Ethephon Treatment on the Formation of Female Flowers and Seeds from Male Plant of Hemp (Cannabis sativa L.)

  • Moon, Youn-Ho;Lee, Yoon Jeong;Koo, Sung Cheol;Hur, Mok;Huh, Yun Chan;Chang, Jae-Ki;Park, Woo Tae
    • Korean Journal of Plant Resources
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    • v.33 no.6
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    • pp.682-688
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    • 2020
  • Hemp (Cannabis sativa L.) is a dioecious plant, although monoecious plants are bred in some cultivars for fiber or seed production. Recently, hemp has received attention for medicinal use with some cannabinoids, including cannabidiol. Self-fertilization for breeding inbred lines is difficult because of dioeciousness and anemophily in hemp. This experiment was conducted to develop a self-fertilization method by forming female flowers and seeds from male plants of dioecious hemp. To induce the formation of female flowers on male plants, 500 mg L-1 of ethephon was sprayed on plants at soon, seven and fourteen days after primordia formation. The plant ratio of female flowers formation and the number of harvested seeds were increased by ethephon treatment. Female flowers of male plants have 5 stigmas in contrast to the dual stigma of female 1plants. Male plant seeds were lighter and smaller than those from female plants. Although the germination rate was lower than that of normal seeds from female plants, the seeds from male plants germinated to grow seedlings. Thus, we suggest that hemp plants should be treated with ethephon at soon after primordia formation to induce the formation of more female flowers on the male plants.

Identification of Major Fungi and Bacterial Species in Solid Medium Drainage for Circulating Hydroponics System (수경재배 배액 재이용을 위한 주요 곰팡이 및 박테리아 종 파악)

  • Lee, Donggwan;Son, Jinkwan;Kang, Taegyeong;Jang, Jaekyung;Park, Minjung;Lee, Taeseok;Lim, Ryugap
    • Journal of Environmental Science International
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    • v.29 no.11
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    • pp.1109-1123
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    • 2020
  • In this study, the amount of harmful fungi and bacteria contained in the drainage and culture medium from the paprika hydroponic facility is identified. In addition, by proposing the necessity of effective purification of discharged drainage, this study attempted to confirm the possibility of drainage reuse. Finally, this study provides basic data on the basis for calculating the need for purification facilities in the future, as well as improvements in horticulture facility for sustainable agriculture. As a result of the analysis, a total of 12 types of fungi were detected in paprika medium and 10 types of fungi were detected in the drainage, and their densities were 130 and 68, respectively. Among the fungi detected in the media and drainage of the paprika hydroponic facility, the fungi with the highest detection frequency are Fusarium, Phytophthora, and Pythium. In the case of bacteria, a total of 2 types of bacteria were detected in the paprika facilities, and the density was 28 and 23, respectively. Therefore, in order to reuse the drainage and settle the circulating hydroponic cultivation system, a water treatment process capable of appropriate treatment is required.