• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.3
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• pp.165-170
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• 2009

A system for the production of transgenic plants has been developed for perennial ryegrass (Lolium perenne L.) via Agrobacterium-mediated transformation. Included in this study were two factors which may affect the gene transfer efficiency: concentrations of acetosyringone (AS, 0 to 300 ${\mu}M$), and co-culture period (1 to 7 days). Both factors were very important to achieve high efficiency gene transformation in the perennial ryegrass. The highest transformation efficiency was obtained when embryogenic calli were inoculated with Agrobacterium in the presence of 100 ${\mu}M$ AS with the culture medium for 5 days. Phosphinothricin resistant calli were developed with into complete plants. GUS histochemical assay, polymerase chain reaction (PCR) and Northern blot analysis of transgenic plants demonstrated that transgenes were integrated into the genome of perennial ryegrass. Using this protocol, it was possible to obtain transformants efficiently for further study.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.2
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• pp.177-182
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• 2004

This experiment was conducted to compare the agronomic characteristics and productivity inintroduced vetch cultivar at the experimental field of Grassland and Forage Crops Division, National Livestock Research Institute from 1999 to 2000. The experiment was arranged in a randomized block design with three replications. The vetch cultivar used in this study were 8 cultivar(Haymaker plus, Common, Capello, Namoi, Victa, Aneto, Popany, Korean milk vetch). Namoi, Victa and Aneto cultivar were died during winter. The cultivar having the fastest flowering date was Korean milk vetch while the flowering date of Common was the latest as 22th May. The average dry matter(DM) content was 14.7% and crude protein(CP) content was 20.9%. Common showed the highest CP content by 23.1% and Korean milk vetch showed the lowest content by 18.0%. Average acid detergent fiber(ADF) and neutral detergent fiber(NDF) content were 33.5 and 42.5% respectively. All cultivar showed the first grade of RFV(relative feed value). The productivity of vetch cultivar were severely different according to the winter hardness. Common showed the highest DM yield by 8,664kg/ha, but the DM yield of Capello and Popany were low by 3,004 and 2,114kg/ha, respectively. The yield of CP and TDN(total digestible nutrient) also was high at Common. The results of this experiments indicated that the productivity of vetch cultivar was effected greatly depending on winter hardness, therefore it was thought winter hardness is the main factor for introducing vetch cultivar.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.3
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• pp.257-265
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• 1996

To obtain the basic informations necessary to develop the production technology of vegetable soybean seeds, this study was conducted at the National Crop Experiment Station, RDA, Suwon, Korea in 1993. Seeds of three vegetable soybean varieties, 'Mikawashima', 'Hwaeomputkong', and 'Seokryangputkong' produced at Pyeongchang, a highland in Korea and introduced from Japan were planted on April 20 in the field. The emergence rate among varieties was significanlty different. Emergence rate of seeds produced at Pyeongchang was higher by 7% than that of the seeds from Japan. Rate of pod infection by Phamapsis spp., brown spot by Septaria glycines, soybean mosaic virus(SMV), and downy mildew by Peronospora manshurica in the field was not different between locations. The growth characteristics, yield components, and green pod and grain yields were not different between locations. Therefore, it was proved that the production of vegetable soybean seeds with the better quality and higher emergence is possible in highland, Korea. possible in highland, Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.2
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• pp.97-104
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• 2005

This experiment was conducted to introduce strip croppings fur decreasing the damage of the continuous com cultivation at the experimental field of Grassland and Forage Crops division, National Livestock Research Institute from 1999 to 2000. Introduced mps for strip cropping to the continuously com cultivated field were red clover (RC), alfalfa(AL), and mixture of grass md legume. Fall panicum(FP), which is one of warm season's weeds was focused on its emerged quantity in com field changed by the level of soil water content and degree of the continuous corn cultivation. The ranges of yearly gained forage fresh yields including weeds like mainly FP were 119.4 ${\~}$ 169.6 in the continuous cultivation with com and rye and 64.9 ${\~}$ 83.3 ton / ha in the corn mono-cropping. Meanwhile, the percentages of pure com in continuous com cultivation plot were only $31-38\%$. This was for the mainly emergence of FP in the continuously com cultivated field. The ranges of average fresh yield of emerging FP were 45.6${\~}$63.4 ton/ ha in the continuously com cultivated field which were over the ranges of yields of rye, 28.2 ${\~}$ 41.6 ton/ ha. The dry matter yields of red clover, alfalfa and mixture with grass and legume introduced for strip cropping were 10.2, 10.6 and 10.6 ton / ha, respectively and these were about the 1/4 average fresh com yield. After strip cropping of red clover, alfalfa and mixture with grass and legume, the yields of corn increased to 56, 82, 88 and 79 ton / ha, respectively and com yield was much better in the alfalfa strip cropping.

• Korean Journal of Poultry Science
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• v.39 no.4
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• pp.283-290
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• 2012

The objective of this study was to compare the growth performance between Korean native layer chickens and imported layer chickens at early rearing stage. Total number of chicks analyzed in this study was 276 and feeding period was conducted from July 24, 2012 for 10 weeks. Five strains including 2 Korean native strains: A=Korean Native Black (Chungcheongbuk-do) and B=Korean Native Yellowish Brown (Gyeongsangbuk-do) and 3 imported layer strains: C=White Leghorn (Gyeongsangnam-do), D=White Leghorn (Seoul), and E=Ameraucanas (Gyeongsangbuk-do) were used to analyze the following traits such as fertility, hatchability, body weight at a different growing stage, average body weight gain, and feed conversion ratio. The fertilities and hatchabilities of strains were 93.88% and 95.65% in strain A, 81.75% and 86.24% in strain B, 82.25% and 88.15% in strain C, 79.25% and 90.85% in strain D, and 71.50% and 88.11% in strain E, respectively. A viability was excellent in strains A and E to be more than 98% and was low in strain D to be 86.67% at a whole week. The strain A had greater body weight during growing stages (p<0.05) than the other strains. The shank length of strain D of $56.69{\pm}3.27mm$ was the highest value at 10 weeks of age among strains (p<0.05). The phenotypic correlation coefficients of strains A and D between an average body weight gain and a shank length were 0.63 and 0.73 during 0~2 wk, 0.70 and 0.55 during 2~4 wk, 0.55 and 0.54 during 4~6 wk, 0.50 and 0.24 during 6~8 wk, and 0.46 and 0.29 during 8~10 wk, respectively. The Korean native hens may have potential abilities to be used as an excellent seed stock for poultry industry.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.2
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• pp.237-241
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• 2010

Many policies have been implemented to mitigate the greenhouse gases in atmosphere overall of sectors. With considering the distinct characteristics of the food security, agricultural sector is no exception to this situation. To this regard, total amount of carbon which is emitted through all of the agricultural production process is calculated, and being based on this result, the demand for the introduction of agricultural production system with low carbon has been rising. Case studies on the application of life cycle assessment (LCA) technique to agricultural sector are found in many countries. For example, life cycle inventory (LCI) data bases of crop, farm infrastructure, fertilizer, farm machinery, and etc., have been constructed and provided by Ecoinvent (Swiss centre for life cycle inventories) of Swiss. In Japan, Top-down typed LCA methodology for agriculture is developed based on the inter-industry analysis, and is evaluated according to the productive method of crop. On the other hand, environmental impact assessment of agricultural system using LCA in Korea is just in the beginning stages. So it is required to assess environmental impact on agricultural fertilizer and pesticide, and to develop their flow modeling, and methodology of LCA of agricultural sector. Environmental impact assessment on agricultural materials, machinery, and infrastructure will also be carried out.

• Journal of Embryo Transfer
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• v.30 no.3
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• pp.207-211
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• 2015

Many pronuclear stage eggs were used to generate transgenic mice (Tg) by microinjection. In this study, we used in vitro fertilized mouse eggs, followed by ultrarapid freezing to establish a simple procedure for production of Tg mice. We produced in vitro fertilized mouse eggs and cryopreserved them by ultrarapid freezing method. A total of 139 cryopreserved-thawed pronuclear eggs, of which 101 (72.6%) were survived following microinjection of chicken ${\beta}-actin$ promoter-driven firefly improved luciferase cDNA (${\beta}-act/luc^+$) and were transferred into 5 recipients. All recipients became pregnant and gave birth to a total of 15 (14.8%) pups. As a control, same DNA construction (${\beta}-act/luc^+$) was also injected into 450 in vitro fertilized eggs, of which 338 (75.1%) were survived and then were transferred into 14 recipients. Eleven (78%) mice became pregnant and littered a total of 54 (19.1%) pups. Southern blotting analysis of Tg mice indicated that one (1/15, 6.6%) and three (3/54, 5.5%) transgenic mice were production from cryopreserved and in vitro fertilized eggs, respectively. All Tg mice produced from both eggs showed the expression of improved luciferase gene. These results indicated that efficiency of produced of Tg mice from cryopreserved eggs was comparable to that from in vitro fertilized eggs. Furthermore, it is suggested that microinjection of transgene into in vitro fertilized eggs cryopreserved by ultrarapid freezing is an easy and conveniently method for production of Tg mice.

• Journal of Life Science
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• v.22 no.9
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• pp.1152-1158
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• 2012

Development of transgenic plant increasing crop yield or disease resistance is good way to solve the world food shortage. However, the persistence of marker genes in crops leads to serious public concerns about the safety of transgenic crops. In the present paper, we developed marker-free transgenic rice inserted high molecular-weight glutenin subunit (HMW-GS) gene ($D{\times}5$) from the Korean wheat cultivar 'Jokyeong' using Agrobacterium-mediated co-transformation method. Two expression cassettes comprised of separate DNA fragments containing only the $D{\times}5$ and hygromycin resistance (HPTII) genes were introduced separately into Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring $D{\times}5$ or HPTII was infected into rice calli at a 3: 1 ratio of EHA105 with $D{\times}5$ gene and EHA105 with HPTII gene expressing cassette. Then, among 66 hygromycin-resistant transformants, we obtained two transgenic lines inserted with both the $D{\times}5$ and HPTII genes into the rice genome. We reconfirmed integration of the $D{\times}5$ and HPTII genes into the rice genome by Southern blot analysis. Wheat $D{\times}5$ transcripts in $T_1$ rice seeds were examined with semi-quantitative RT-PCR. Finally, the marker-free plants containing only the $D{\times}5$ gene were successfully screened at the $T_1$ generation. These results show that a co-infection system with two expression cassettes could be an efficient strategy to generate marker-free transgenic rice plants.

• The Korean Journal of Mycology
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• v.38 no.1
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• pp.48-53
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• 2010

Agrobacterium-mediated transformation was conducted in order to generate DNA insertional mutants of Flammulina velutipes. Agrobacterium tumefaciens AGL-1 harboring pBGgHg was transformed into gill tissues of Flammulina velutipes strain KACC42777. The transformants resistant on hygromycine ($30\;{\mu}g/ml$) were confirmed by PCR. The targeted insertional sites were amplified by inverse PCR and sequenced. To find the phenotype variation of all generated transformants, bottle cultivation which followed by the standard cultivation protocol were conducted. Color variation was observed on the cultivated fruiting bodies. Furthermore, the transformant pool will be used as a good genetic resources for studying gene function.

• Journal of Korean Library and Information Science Society
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• v.46 no.2
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• pp.317-336
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• 2015

This paper provides a brief overview of bibliographic relationships, a analysis of bibliographic relationships' characteristics of 2,940 bibliographic records on the Five Classics and Four Books taken from Korean National Bibliography, and a methodology for applying the FRBR model based on analysis of the samples. As results of analysis, the work type ranked high particularly in the commentary, translation with notes, and translation on the original works etc. I suggested adopting conception of supper works, that is, family of works and using uniform title as a connective device between the works, that is, an authorized access point of RDA to apply FRBR model of the Five Classics and Four Books. Also I exemplified the description of uniform title in 630 field as subject added entry.