• Journal of Sericultural and Entomological Science
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• v.16 no.1
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• pp.77-84
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• 1974

In autumn season residual period of nicotine is observed in the larval duration, weight of molting larvae at the various stages, percentage of delayed molting larvae, and survival rate up to the 3rd stage from the 1st season to the 3rd season and up to the stage for four seasons, respectively. Mulberry tree is planted in pot from June 18, 1973 to August 4, 1973 and placed every other row between tobacco variety of Hicks cultivated in mulching system and control is prepared in the Sericultural Experiment Station, placing in the every ridge of mulberry field with the four seasons ; 5 day isolation (1st season), 15 day isolation (2nd season), 25 day isolation (3rd season), and 50 day isolation (4th season). 1) 1st and 3nd instar larvae fed with the tobacco stained mulberry leaf has longer larval duration for the four seasons, as compared with those of the control, increasing 12％ in index with 28 hours, 17％ in index with 37 hours, 23％ in index with 50 hours, 17％ in index with 43 hours from the 1st season to the fourth season and the 3rd to the 4th instar larvae, increasing 18％ in index with 55 hours. 2) 2nd instar molting larvae, 3rd instar molting larvae from the 1st to the 4th season and 4th instar molting larvae at the 4th season decrease their larval weight respectively, as compared with the control. 3) Percentage of delayed molting larvae from the 3rd stage to the 4th stage goes up higher than that of the control in the End larval stage at the 3rd to the 4th season, in the 3rd larval stage at the 1st, the End, and the 4th season, and in the 4th larval stage at the 4th season, respectively. 4) Survival rates in the 2nd larval stage at the 2nd season to the 4th season, in the 3rd larval stage at the 1st to the 4th season, and in the 4th larval stage at the 4th season respectively are higher than that in the control.

• Clinical and Experimental Reproductive Medicine
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• v.15 no.2
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• pp.103-117
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• 1988

Ovulation induction was done with 3 different regimens as clomid combined with HMG, HMG only, and FSH combined with HMG in 28 menstrual cycles for IVF-ET and GIFT program. The appearance of endogenous LH surge, estradiol plateau, atypical LH surge, and time from initiation to peak of LH surge in serum and urine were observed and compared in 3 groups. 1. The estradiol concentration of serum LH surge day was similar in three groups but 1st group (Clomiphene Citrate+Sequential HMG) was slightly higher at $1924.0{\pm}865.1\;pg/ml$. In regards to the existence of serum estradiol plateau, 3rd group (FSH+Sequential HMG) was highest at 60%, and 1st group and 2nd group (HMG only) were similar at 33% and 44% respectively. 2. The number of ovarian of ovarian follicle which was more than 18mm in diameter was $4.1{\pm}2.0$, $4.2{\pm}2.1$ respectibely for 2nd group and 3rd group. Although the numbers were slightly higher thean 1st group for each ovarian follicle, serum estradiol value per follicle was higher for 1st group at $583.0{\pm}261.2pg/ml$. 3. When measuring the urine LH surge according to Hi-Gonavi and according to the standard set by three different types of surge, simultameous satisfaction for 1st group, 2nd group, 3rd group was two cases, five cases, four cases respectively at 40%, and the remained cases were composed of numorous type combination which satisfied the two definition, simultaneously in this study, the LH surge starting time was determined only in the cases tow combination were satisfied simultaneously at first, but there are something to study more. In one case of the 3rd group. 4. The concentration of LH surge start in urine and serum of 2nd group was highest at306. $0{\pm}287.2IU/l$ and $34.0{\pm}9.9mIU/ml$ and 1st group was low at $116.6{\pm}66.1IU/l$ and 7.4mIU/ml. The urine and serum value of LH was highest at $1644.4{\pm}988.8IU/l$, $65.9{\pm}15.0mIU/ml$ for 2nd group, 1st group was low at urine, and 3rd group was low of serum. With pregnancy established, the LH concentration of urine was relatively high but on the contrary the LH concentration of serum was low compared to urine concentration. 5. Time from LH surge start to the maximun of urine and serum value was highest at 15. $7{\pm}9.1$ hrs and $10.8{\pm}4.9$ hrs for 1st group and 3rd group. With pregnancy established, time was shortened for urine but on the contrary serum showed an increase in time. 6. The concentration of LH which increases with time on urine was highest at 2nd group ($194.6{\pm}76.8\;IU/hour$). The lowest increase for serum was at 3rd group (2.1mIU/hour). With pregnancy established, urine showed more increase than control group ($266.5{\pm}47.4\;IU/hour$) and for serum there was similar increase ($3.4{\pm}0.8\;mIU/hour$). 7. There were two examples of non-typical surge from 1st group and 3rd group each. Among these three cases showed a reestablishment of LH surge after 10-24 hours. 8. Endogenous spontaneous Lh surge occurs once for each 2nd group and 3rd group.

• Journal of Embryo Transfer
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• v.29 no.3
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• pp.229-234
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• 2014

The aim of the present study was to compare two different serum-free media, modified synthetic oviduct fluid (mSOF) and modified potassium simplex optimization medium (mKSOM) containing 20% RD (RPMI1640 + DMEM, 1:1 v/v) (RD-mKSOM), for in vitro culture (IVC) of bovine embryos. After in vitro maturation and fertilization, the presumptive zygotes were cultured in two different serum-free conditions for 7 days and 9 days to evaluate blastocyst formation and hatching, respectively. Serum supplemented conventional CR2 medium was used as control. After 7 day of culture, there was no significant difference in cleavage and blastocyst formation rates among three groups (mSOF, 59.3 and 30.1%; RD-mKSOM, 65.0 and 41.5%; control, 51.6 and 38.0%, respectively). Hatching rate was significantly higher in control (69.0%) than other experimental groups (mSOF, 22.0%; RD-mKSOM, 39.5%) (P<0.0001 and P<0.001, respectively). Although both serum-free conditions showed lower hatching rates than serum-added control, in serum-free groups, RD-mKSOM showed significantly higher hatching rate than mSOF (P<0.001). In addition, one-step using RD-mKSOM may facilitate IVC procedure than two-step culture system. In conclusion, the results indicate that one-step RD-mKSOM is more suitable defined culture system for IVC of bovine embryos than two-step mSOF.

• The Journal of Internal Korean Medicine
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• v.27 no.2
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• pp.345-355
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• 2006

Objectives : The purposes of this study are to evaluate the effect of treatment in obesity clinic in Dae-Gu Hanny University and to improve the oriental medical methods of treatment in obesity patients. Methods : 62 patients were classified into four groups by Body Mass Index(BMI) : normal, overweight, 1st obesity and 2nd obesity groups. Weight, BMI, body fat rate and abdominal fat rate were compared in each group by t-test. Results : In distribution of sex, male were 10(16.1%), female were 52(83.9%). Mean age was $31.0{\pm}2.59$ and the patients in twenties and thirties were the most. Mean weight was $71.80{\pm}4.08kg$ and 1st obesity group(23) was the most. Mean BMI was $27.11{\pm}1.20(kg/m^2)$. BMI Changes in obesity groups treated by obesity clinic were $0.95{\pm}0.68kg/m^2$ in normal group, $1.45{\pm}0.38kg/m^2$ in overweight group, $2.67{\pm}0.48kg/m^2$ in 1st obesity group and $4.01{\pm}1.10kg/m^2$ in 2nd obesity group. The changes in all groups were significant. The more the obesity degree was. the more weight loss was. Conclusions : The weight and BMI in patients were almost decreased significantly when they treated. It resulted that the effect of oriental medical treatment in obesity was positive. This study is the effect of treatment in obesity by several medical methods and it is thought that the study of the effects of treatment in obesity by each medical methods that were used in this study is necessary.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.1
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• pp.1-10
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• 2023

This study aimed to confirm the importance ratio of climate and management variables on production of orchardgrass in Korea (1982-2014). For the climate, the mean temperature in January (MTJ, ℃), lowest temperature in January (LTJ, ℃), growing days 0 to 5 (GD 1, day), growing days 5 to 25 (GD 2, day), Summer depression days (SSD, day), rainfall days (RD, day), accumulated rainfall (AR, mm), and sunshine duration (SD, hr) were considered. For the management, the establishment period (EP, 0-6 years) and number of cutting (NC, 2nd-5th) were measured. The importance ratio on production of orchardgrass was estimated using the neural network model with the perceptron method. It was performed by SPSS 26.0 (IBM Corp., Chicago). As a result, EP was the most important variable (100%), followed by RD (82.0%), AR (79.1%), NC (69.2%), LTJ (66.2%), GD 2 (63.3%), GD 1 (61.6%), SD (58.1%), SSD (50.8%) and MTJ (41.8%). It implies that EP, RD, AR, and NC were more important than others. Since the annual rainfall in Korea is exceed the required amount for the growth and development of orchardgrass, the damage caused by heavy rainfall exceeding the appropriate level could be reduced through drainage management. It means that, when cultivating orchardgrass, factors that can be controlled were relatively important. Although it is difficult to interpret the specific effect of climates on production due to neural networking modeling, in the future, this study is expected to be useful in production prediction and damage estimation by climate change by selecting major factors.

• Journal of Microbiology and Biotechnology
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• v.23 no.12
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• pp.1802-1805
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• 2013

Ginsenosides are the most important ingredient of ginseng and are known to possess many pharmacological and biological effects. Rb1, a major protopanaxadiol ginsenoside, is the most abundant ginsenoside in Panax ginseng C.A Meyer and can be hydrolyzed into more pharmaceutically potent minor ginsenosides. To identify a microorganism that is capable of converting Rb1 into other ginsenosides, we screened 12 Microbacterium spp., and M. trichothecenolyticum was identified as a likely candidate. M. trichothecenolyticum converted Rb1 into Rd and then into Rh2 based on TLC and HPLC analyses of reaction products. This biotransformation method can be easily applied for mass production of Rd and Rh2 by using Rb1.

• MALSORI
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• no.31_32
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• pp.9-22
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• 1996

This study aims to describe the acoustic characteristics of Korean nasal sounds making use of the notion of pole and zero. In case of [m], the 1st and 4th formant almost remains as the original shape respectively, on the contrary, the 2nd and 3rd formant were observed as a variable cluster together. Alveolar [n] shows that the 3rd and 4th formant make a variable cluster with their antiformant(zero), however, the 1st and 2nd formant keep the static shape of their on. Velar [$\eta$] has 4 formants below 2900 Hz and the 3rd and 4th formant constitute a variable cluster together as does the case [n]. With respect to the energy distribution in case of [n] and [$\eta$], the energy value diminishes from Fl up to F3 continuously but augments in F4. The [m] shows that in the region of Fl-F2 does the energy fall down and rise from F3 to above.

• The Journal of Internal Korean Medicine
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• v.25 no.2
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• pp.214-223
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• 2004

The study examines the anti-cancer effects of the hot water extract of Mylabris phalerata(MP) using SNU-C5 cell lines. Microscopic analysis showed that 12 hours after MP treatment, the number of dead cells increased prominently. Significant cell death was observed 12, 24, and 48 hours after MP treatment through trypan blue exclusion testing. This suggests that MP is time-dependently cytotoxic. Mitotracker Red CMXRos staining and flowcytometry revealed that MP decreased mitochondrial membrane potentials. The absence of peaks on PI staining showed that DNA damage occurred in MP treated cells. Taken together, measurements suggest that MP has a strong anti-cancer effect on SNU-5 cell lines, and that this is likely to be due to the destruction of mitochondria and DNA damage.

• The Journal of Dong Guk Oriental Medicine
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• v.1
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• pp.237-249
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• 1992

In order to study tile effects of moxibustion on renal failure induced by $HgCl_2$ in rats aminal experiments were carry out. The moxibustion was treat into Sin-soo locus(VII23), We-soo locus(VII21) and Sin-soo + We-soo loci these loci were correspond to human body. Experimental group were divided into 4 groups : lontrol group, moxibustion groups treat on Sin-Soo and We-Soo, Sin-Soo + We-Soo group. The obtained results were as follows : 1. The concentration of serum sodium were inreased in all the groups, compared with those of the control group. Sin-Soo, We-Soo, Sin-Soo + We-Soo loci showed increased on the 1st and 3rd days. All three experimental groups showed a significant increased on 3rd day. 2. The concentration of serum potassium were decreased in all the group, compared with those of the control group. Sin-Soo, We-Soo showed a significant decrease on 3rd day. Sin-Soo + We-Soo showed a little decrease but do not significant decrease. 3. The levels of serum total protein were increased in all the group, compare with those of the control group. Sin-Soo, We-Soo show a significant increased or 3rd day. Sin-Soo + We-Soo do not showed a significant increased. 4. The levels of serum albumin were decreased in all the groups, compared with those of the control group. Sin-Soo, Sin-Soo + We-Soo showed a significant decrease on 3rd day. We-Soo does not showed a significant decrease. 5. The concentration of serum blood urea nitrogen were decreased in all the groups, compared with those of the control group. Sin-Soo, We-Soo, Sin-Soo + We-Soo showed a significant decrease on 3rd day. 6. The levels of serum alkaline phosphatase were decreased in all the group, compared with those of the control groups. Sin-Soo, We-Soo showed a significant decrease on 3rd day Sin-Soo + We-Soo not a significant decrease. In conclusion, the result of this study seemed to effect on the treatment for renal failure induced by $HgCl_2$.

• The Journal of Internal Korean Medicine
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• v.26 no.2
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• pp.360-368
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• 2005

Background & Object: The aim of this study was to investigate the effects of Traditional Korean medical therapy on gastric myoelectrical activity in patients with functional dyspepsia. Methods: 29 patients(male 10, female 19) were divided into two groups; Inpatient group(N=10, male 1, female 9) and outpatient group(N=19, male 9, female 10). 10 healthy people participated in this study as normal controls. Gastric motility was recorded and analyzed by electrogastrography(EGG) during fasting and postprandial periods. Hospital treatment included electroacupuncture on ST36, acupuncture, and herbal medicine. Outpatients were treated with herbal medicine and acupuncture. Inpatients underwent hospital treatment for two weeks, and after discharge they were treated as outpatients for six weeks. In inpatient group, EGG was recorded before and after hospital treatment and it was recorded at six weeks after discharge. In outpatient group, EGG was recorded before starting treatment and it was recorded once a every six weeks for twelve weeks. Results: There was no significant difference in EGG parameters between before and after therapy in outpatient group. In inpatient group, postprandial normal slow wave increased after hospital treatment(from $33.94{\pm}14.13$ to $62.63{\pm}20.18$, p<0.05) and postprandial tachygastria decreased(from $43.53{\pm}13.99$ to $28.30{\pm}16.63$, p<0.05) and arrhythmia decreased(from $20.35{\pm}15.91$ to $5.67{\pm}6.80$, p<0.05). Conclusions: Results suggest that hospital treatment through electroacupuncture on ST36, acupuncture, and herbal medicine is useful to improve gastric myoelectrical activity in Patients with Functional Dyspepsia.