• 대한수의학회지
• /
• 제41권1호
• /
• pp.43-49
• /
• 2001

As compared with reaction of antibody for sonicated antigen of Brucella abortus strain RB51 and 1119-3 by Western blot analysis, Brucella field positive sera was detected strong reaction at 40~80 kDa LPS of strain 1119-3, but detected very weak reaction at strain RB51 partly. Otherwise, as we analyzed major immunogen of RB51 by antisera bled periodically during 6 months after RB51 vaccination. we detected strong immunological reaction at 17, 18 and 8 kDa antigen of RB51. Especially, reaction of 8 kDa antigen by Western blot coincided with reaction of dot-blot assay in RB51-antibody detection method. We also compared with reaction of field sera by STAT(standard tube agglutination test), dot-blot assay and Western blot (reaction of 8 kDa antigen of strain RB51). 16 sera of 4~5 months after RB51 vaccination are all negative by STAT, and 12 field brucellosis positive serum are all positive, and also 12 of 16 sera vaccinated RB51 are positive by dot-blot assay and reaction of 8kDa antigen by Western blot. but 1 of 15 Brucellosis negative sera reacted nonspecifically dot-blot assay.

• 대한수의학회지
• /
• 제40권3호
• /
• pp.543-549
• /
• 2000

The pathogenicity of Br abortus RB51 strains, producted by commercial vaccine companies in Republic of Korea and USA, were evaluated in mouse and guinea pig. BALB/c and ICR mice were intraperitoneally inoculated with RB51 vaccines or virulent field strain and the existence of RB51 including its ratio of spleen to weights and persistence in spleens were examined. Groups of guinea pigs on day 55-58 of received subcutaneously with various RB51 vaccines, RB51 field isolates (Daehungjin) or virulent field isolates(Sangju) to compare the histopathogenicity in uterus. All the mice received RB51 vaccines or RB51 field isolates survived for 10 days, but the groups of mice received virulent field isolates died 5 from 11 (45.5%) in case of BALB/c mice and 12 from 12 (100%) in ICR mice, respectively. The number of RB51 in the groups of mice given with vaccine strains and RB51 field isolates were declined rapidly were in spleens between 12 and 20 days after inoculation. In contrast the mice given with the virulent field isolates rose in number of bacteria up to 20 days after inoculation. In the groups of mice infected with virulent field isolates, the ratio of spleen weights to body weight were significantly higher than those in control or in the groups inoculated with RB51 strain, including RB51 field isolates, at 12 and 20 days after inoculation. At ten days after inoculation, placentas of both the pregnant and non-pregnant guinea pigs were conducted for histopathological examination. Although any abnormal lesions were not observed in non-pregnant guinea pigs, all the strains caused the inflammation of the placenta, implying pathogenecity of RB51 in pregnant guinea pigs.

• 대한수의학회지
• /
• 제40권3호
• /
• pp.533-541
• /
• 2000

Biochemical and genetic analysis were carried out to investigate the potential recovery of pathogenecity or related mutations of Brucella abortus RB51 vaccine strains. RB51 strains were recovered from commercial vaccines, including related seed stocks from private companies in Republic of Korea, strain from USA, a reference strain from C university and a field isolate (Daehungjin) from aborted dairy cow after RB51 vaccination were compared with two identified virulent wild strains (S2308 and a field strain isolated from dairy cow in Korea) at the same conditions. All the strains examined, except identified pathogenic strains, revealed the identical characteristics to the original RB51 in biochemical properties, antigen and bacteriophage typing. Outer membrane protein (OMP) profiles from strains of RB51 showed the same patterns with standard RB51 in SDS-PAGE. In addition, Western blotting with the brucella specific monoclonal antibody also indicated that all the vaccine strains were completely deficient in their LPS compared to the pathogenic Br abortus strains. The differences in DNA structures among strains were also possible to detect after PCR. All vaccine strains, except S19, S1119-3, S1075, S544 and Br suis, were amplified a 178bp DNA fragment of eri-gene, and 364bp of IS711 elements. In contrast, 498bp DNA product was only found with Br abortus. Overall evidences in the present study confirmed that the RB51 strains for vaccine production in Korea did not originated from the phenomena of possible recovery of pathogenicity or related to any potential mutation event at all.

• 대한수의학회지
• /
• 제41권1호
• /
• pp.51-57
• /
• 2001

A procedure for extraction and purification of 8 kDa antigen of Brucella abortus RB51 was developed. Bacteria heat inactivated at $60^{\circ}C$, 30 min was extracted by 1% sarcosine and followed by fluid pressure liquid gel filtration chromatography of 2 series, Superose 12 HR 10/30 and Sephacryl S-100. There was produced $71.46{\mu}g/g$(wet) of 8 kDa antigen, and it resisted 1% trypsin, solved 1% triton X-100 higher than distilled water and inactivated 0.1% proteinase K. These results show that 8 kDa antigen may be a lipoprotein existed cell surface of B. abortus RB51. Also, we developed ELISA using purified 8 kDa surface antigen of Brucella abortus RB51 strain, its specificity and sensitivity was 95.0%, 98.6%, respectively. As compared with dot-blot assay using whole cell and ELISA using 8 kDa antigen, its correlation was 93.5%.

• 월간낙농육우
• /
• 제18권11호통권199호
• /
• pp.77-79
• /
• 1998

지난 6월 제주도에서 소 부루세라 백신 RB51을 접종한 한우 500여두가 유산을 일으켰으며, 7월 이후 경기, 강원, 충남지역의 일부 낙농농가의 젖소가 유산 및 조산을 일으켜 IMF로 시름이 커진 축산농가, 특히 낙농가의 괴로움이 더해지고 있는 사실이 정말로 가슴아프다. 이 기고에서 나는 최근 발생되고 있는 ${\ulcorner}$젖소의 유$\cdot$조산 원인 및 부루세라 백신(RB51)의 안전성 평가를 위한

• 대한수의사회지
• /
• 제34권9호
• /
• pp.645-649
• /
• 1998

• 자원환경지질
• /
• 제25권3호
• /
• pp.245-258
• /
• 1992

The variations of certain major and trace elements of the Onjong granite mass was studied on the basis of petrological and geochemical characteristics and compared with those of the Eonyang-Yucheon granite masses in order to investigate the geochemical differences of the granitic rocks in relation to mineralization between Pb-Zn ore district and Pb-Zn-Mo-W ore district in Kyeongsang basin. The Onjong granite mass is classified into granodiorite and monzo-granite, and the Eonyang-Yucheon granite masses into monzo-granite by the Streckeisen's diagram. Between both granite masses there are clear differences in contents of certain major elements and lithophile trace elements. The former have high contents of Ca (2.94%), Mg (1.66%) and Sr (365 ppm), and low contents of K (3.52%), Na (3.51%), Rb (116 ppm), Ba (640 ppm) and Li (18.9 ppm), whereas the latter have high contents of K (4.02%), Na (4.28%), Rb (145 ppm), Ba (695 ppm) and Li (19.3 ppm), and low contents of Ca (1.42%), Mg (0.43%) and Sr (161 ppm). Except for Mo, there are not clear differences in chalcophile trace elements between two granite masses: the Onjong granite mass has higher Mo content (7.1 ppm) lnan that (1.7 ppm) of the Eonyang-Yucheon granite masses, but Pb and Zn contents are similar between the Onjong granite mass (Pb=8.7 ppm, Zn=37.1 ppm) and the Eonyang-Yucheon granite masses (Pb=7.8 ppm, Zn=39.8 ppm). Ca and Sr contents of the Onjong granite mass (Ca> 1.5%, Sr> 270 ppm) are higher than those of the Eonyang- Yucheon granite masses (Ca<1.5%, Sr<270ppm), and Rb/Sr, Rb-Rb/Sr and K-Rb/Sr ratios are clearly distinguishable between the Onjong granite mass(Rb/Sr<0.51, Rb-Rb/Sr>250 and K-Rb/Sr>5.2) and the Eonyang- Yucheon granite masses (Rb/Sr>0.51, Rb-Rb/Sr<250 and K-Rb/Sr<5.0). Thus, variations of certain major and trace elements and ratios are applicable as geochemical index to distinguish the types of mineralization of the ore districts related to the Cretaceous granitic rocks in the Kyeongsang basin.

• 대한화학회지
• /
• 제51권3호
• /
• pp.265-269
• /
• 2007

국부적인 결정구조와 에너지 띠 간격 간 상관관계를 고찰하기 위하여 Nb를 포함하는 일련의 불소 산화물에 대하여 결정구조와 자외선-가시광선 확산 반사율 스펙트럼을 비교, 연구하였다. 이 실험에서 다룬 RbSrNb2O6F와 RbCaNb2O6F, RbNb2O5F는 공통적으로, 꼭지점 공유를 하고 있는 NbO5F 팔면체로 구성되어 있 다. 구조적 뒤틀림 정도의 척도로 볼 수 있는 Nb-O(F)-Nb 평균 결합각은 RbSrNb2O6F에서 158.6°, RbCaNb2O6F 에서 149.6° 그리고 RbNb2O5F에서 139.5o이다. 확산 반사율 스펙트럼으로부터 구한 에너지 띠 간격은 Nb-O(F)-Nb 결합각이 감소할수록 증가하는 경향을 보였다. 즉 RbSrNb2O6F, RbCaNb2O6F, RbNb2O5F 각각의 화합물에 대해 서 3.48 eV, 3.75 eV, 4.03 eV 의 값을 나타내었다. 이러한 실험적 결과는 Nb를 포함하는 불소 산화물에서 국 부구조의 변화를 통해 띠 간격을 약 0.6 eV의 범위에서 조절할 수 있음을 의미한다.

• 대한핵의학회지
• /
• 제6권1호
• /
• pp.51-59
• /
• 1972

$^{86}Rb$ uptake of some organs and tissues, ego both lungs, both renal cortices. small intestine, liver and skeletal muscle were studied in the control and the rabbit subjected to pneumothorax. $^{86}Rb$ in the form of chloride mixed with physiological saline was intravenously. injected. The doses were $100{\mu}c$ for a rabbit. The rabbits were sacrificed at intervals of 10, 20, 40, and 60 seconds after the injection of $^{86}Rb$, by the injection of saturated KCI solution. After sacrification, the organ and tissue sample were quickly removed. $^{86}Rb$ uptake in gm of the organs and tissues were measured. On the basis of uptake value, administered doses and body weight, % dose/gm tissues per 200 gm body weight was calculated. Followings were the results; 1. Pneumothorax resulted in a marked elevation in $^{86}Rb$ uptake value of collapsed lung and returned to normal level lately. 2. Contralateral lung of pnemothorax also showed marked elevation in $^{86}Rb$ uptake value and recovered to normal level. 3. Initial $^{86}Rb$ uptake value of liver, small intestine of the rabbit with pneumothorax showed some elevation as compared to control, but that of late stage were similar with control. 4. Local blood flow determination by means of $^{86}Rb$ uptake were inadequate in the collapsed lung of pneumothorax. 5. It was suggested that the mechanism for the initial elevation of $^{86}Rb$ uptake value in each organs and tissue were different from each other.

• 센서학회지
• /
• 제12권1호
• /
• pp.1-9
• /
• 2003

CsI에 활성제로 Li, K, Rb를 첨가하여 CsI(Li), CsI(K) 및 CsI(Rb) 단결정을 Czochralski방법으로 육성하였다. $^{137}CS$(0.662 MeV)에 대한 CsI(Li:0.2 mole%) 섬광체의 에너지 분해능은 14.5%이었고 CsI(K:0.5 mole%) 섬광체는 15.9%이었으며 CsI(Rb:1.5 mole%) 섬광체는 17.0%이였다. 이들 CsI(Li), CsI(K) 및 CsI(Rb) 섬광체의 $\gamma$선 에너지에 대한 에너지 교정곡선은 선형적 이였다. 일정비율 시간분석법(CFT :constant-fraction timing method)으로 측정한 CsI(Li:0.2 mole%), CsI(K:0.5 mole%) 및 CsI(Rb:1.5 mole%) 성광체의 시간 분해능은 각각 9.0 ns, 14.7 ns 및 9.7 ns이였다. CsI(Li:0.2 mole%), CsI(K:0.5 mole%) 및 CsI(Rb:1.5 mole%) 섬광체의 형광감쇠시간은 각각 ${\tau}_1=41.2\;ns$, ${\tau}_2=483\;ns$, ${\tau}_1=47.2\;ns$, ${\tau}_2=417\;ns$ 및 ${\tau}_1=41.3\;ns$ 및 ${\tau}_2=553\;ns$이였다. 그리고 CsI(Li:0.2 mole%), CsI(K:0.5 mole%) 및 CsI(Rb:1.5 mole%) 단결정의 인광감쇠시간은 각각 0.51 s, 0.57 s 및 0.56 s이였다.