• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.60-60
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• 2020

To establish in vitro axillary bud culture conditions of Echinosophora koreensis Nakai, one of Korean endemic endangered species famous for beautiful flowers, we tested the influence of plant growth regulators (PGRs) in shooting and rooting stage from in vitro plants. In shoot multiplication, addition of 6-benzylaminopurine (BA) to the media induced 2.5 to 3 shoots per bud during 4 weeks of culture. And media including 0.5 mg L^-1 thidiazuron (TDZ) produced 3 to 4 shoots per bud. However, zeatin and isopentenyl adenine (2-ip) were not successful to increase shoot number, and the combination treatments of BA with other PGRs were also not effective. Shoots were smaller than 2 cm in length, in most of the treatments. In rooting, naphthalene acetic acid (NAA) treatments in the range of 0.5 to 4.0 mg L^-1 appeared to increase rooting rate by 10% to 60% approximately when compared with the control but roots developed with callus clusters. Indole butyric acid (IBA) addition had little effect on rooting (below 10%), while some roots were longer than in NAA treatments and some shoots were longer on high IBA concentrations (4.0 to 8.0 mg L^-1). It is suggested that micropropagation is a highly applicable and promising to multiplication and conservation of rare and endangered endemic species.

• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.90-109
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• 2016

This study aims to investigate the flora of Mt. Cheonma (Namyangju-si, Gyeonggi-do, Korea), including Korean endemic, rare and endangered plants. To support the biodiversity and usefulness of this investigation, we checked the red list plants, floristic regional indicator plants, climate change adaptation target plants and naturalized plants, and compared composition of plant species in Mt. Cheonma with nearby mountains. Totally, we performed 22 field investigations from April 2012 to October 2014. The number of vascular plants in this region is 433 taxa and comprised of 94 families, 266 genera, 378 species, 2 subspecies, 46 varieties, and 7 forms. Among them, there are 13 endemic plants designated by Korean Forest Service, whereas only 10 taxa are endemic species by Korean Ministry of Environment (KME). According to the IUCN criteria, there are 12 rare and endangered plants composed of 9 Least Concerned (LC), 2 Vulnerable species (VU), and one Endangered species (EN) found by Korea National Arboretum (KNA). Meanwhile, 8 taxa including 7 Least Concerned (LC) were found in Red List of KME. The floristic regional indicator plants designated by KME were 49 taxa. In addition, there are 14 taxa in The Adaptable to Climate Change Plant List designated by KNA, while only 19 taxa are naturalized plants. Generally, there are 190 common taxa in Gwangju Mountains area, and only 51 taxa are distributed in Mt. Cheonma.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.4
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• pp.25-40
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• 2011

The vascular plants in Jeokjabong, Bogil Island were listed 350 taxa composed of 89 families, 233 genera, 309 species, 1 subspecies, 36 varieties and 4 forms. Divided into woody plants were 142 taxa (40.6%) and herbaceous plants were 208 taxa (59.4%). Based on the list of rare and endangered species by Korea Forest Service and Korea National Arboretum, 3 taxa were recorded; Celtis choseniana, Asarum maculatum, Rubus sorbifolius, and endemic plants, 10 taxa were recorded; Asarum maculatum, Cephalotaxus koreana, Carpinus coreana, Celtis choseniana, Lespedeza maritima, Indigofera koreana, Dendropanax morbifera, Ligustrum quihoui var. latifolium, Weigela subsessilis, Carex okamotoi, and plant species subject to permission for taking abroad, 4 taxa were recorded; Celtis choseniana, Asarum maculatum, Vaccinium oldhami, Carex okamotoi. Based on the list of floristic regional indicator plants by Korean Ministry of Environment were total 64 taxa (18.3% of all 350 taxa of vascular plants); Ligustrum quihoui var. latifolium in class IV, 19 taxa (Stauntonia hexaphylla, Cinnamomum japonicum, Zanthoxylum ailanthoides, Daphniphyllum macropodum, Centella asiatica, Verbena officinalis, Mitchella undulata, etc.) in class III, 5 taxa (Potentilla dickinsii, Ostericum melanotilingia, Sanicula rubriflora, Caryopteris incana, Teucrium veronicoides) in class II and 39 taxa (Gleichenia dichotoma, Cyrtomium fortunei, Aphananthe aspera, Rubus hirsutus, Meliosma myriantha, Hedera rhombea, Ainsliaea apiculata, etc.) in class I. Based on the list of naturalized plants, 5 families, 17 genera, 20 taxa (Phytolacca americana, Lepidium virginicum, Robinia pseudo-acacia, Verbesina alternifolia, Lolium perenne, etc.) and naturalization rate was 5.7% of all 350 taxa of vascular plants. Hereafter natural ecosystem into disturbance on naturalized plants and legal protection species and rare and endangered species distribute in the Bogil Island, precision investigation after management counterplan of the monitoring a point of view be required.

• Journal of Environmental Science International
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• v.6 no.2
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• pp.165-171
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• 1997

The floristic characteristics and ecological conservation measures for the plant resources of the Mt. Kumjung(801m), Pusan, Korea were discussed. The field works was done for about twenty times in nine major areas from March, 1995 to Octover, 1996. The total number of vascular plants In this area were summarized as 103 families, 312 genera, 493 species and 564 kinds. The fourteen kinds of artificially planted, ten kinds of Korean endemics and twenty-nine kinds of Korean rare and endangered species in the area were revised and compiled in the list. The flora of the area were categorized into the South-coast put of the Korean Region, where the species such as Platycarya strobilacea, Quercug serrata, Eutya japonica, Camellia japonica, Trachelosperm urn asia talcum vu . in termedium, Quercus acu tissima were roles as major floristic components in the Korean Region and endemized in the south-cosat part of Korea. The natural communities such as Pinus thunbergii Community, Quercus mongolica community, Cupinus laxiflora Community, Wistria floribunda Community and swamp vegetation in Bukmun area as well as upland grassland at the summit of Kodangpong were represented for their floristic values in the area. The thirty kinds of medichinal plant, fifteen kinds of ornamental plants and thirteen kinds of edible plants are faced potentially in endangered from the outrageous collections by the local peoples. The action plan for the conservation management of the area which enables sustainable use by the local peoples must have established in the near feature.

• Journal of Plant Biotechnology
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• v.36 no.1
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• pp.13-17
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• 2009

Various plant growth regulators were tested for shoot proliferation of Tripterospermum japonicum, a rare and endangered species. Among the six different media tested, MS medium was the best for the shoot growth. Whereas BA, upto 3 mg/L, significantly increased shoot proliferation rate, it suppressed the rate at higher levels. Neither kinetin nor TDZ was so effective in proliferating shoots as BA. As for rooting, TDZ strongly inhibited it even at very low concentration though spontaneous rooting was frequently observed from the proliferated shoots during culture of lower concentration BA or kinetin. In contrast, shoot elongation was significantly promoted by $GA_3$. More than 90% of the proliferated plantlets could be transplanted via cuttings into pots containing artificial soil mixture where they rooted and resumed normal growth. Most of the plants bloomed to bear fruits in the following year.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.1
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• pp.89-105
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• 2012

This study is carried out to survey and analyse the vascular plants distributed around the Jusan Reservoir, Juwangsan national park. The results are as follows. The numbers of vascular plants were 377 taxa; 85 families, 253 genera, 332 species, 3 subspecies, 37 varieties and 5 forma. The endangered plants designated by Ministry of Environment were 2 taxa; Berchemia berchemiaefolia (Makino) Koidz. and Iris odaesanensis Y.N.Lee. The rare plants designated by Korea Forest Service were 6 taxa; Eranthis stellata Maxim., Aristolochia contorta Bunge, Aristolochia manshuriensis Kom., Berchemia berchemiaefolia (Makino) Koidz., Scopolia japonica Maxim. and Iris odaesanensis Y.N.Lee. The endemic plants were 4 taxa; Carpinus laxiflora (Siebold & Zucc.) Blume, Pseudostellaria coreana (Nakai) Ohwi, Philadelphus schrenkii Rupr. and Weigela subsessilis (Nakai) L.H.Bailey. The specific plants by floristic region were 38 taxa; Juglans mandshurica Maxim., Salix chaenomeloides Kimura, Hylomecon vernalis Maxim., Lonicera praeflorens Batalin, Erythronium japonicum (Baker) Decne., Potentilla dickinsii Franch. & Sav., Celtis aurantiaca Nakai, Anemone reflexa Steph. & Willd., Cimicifuga heracleifolia Kom. and so forth. The naturalized plants were 20 taxa; Fallopia dumetorum (L.) Holub, Chenopoidum album L., Lepidium apetalum Willd., Veronica persica Poir., Conyza canadensis (L.) Cronquist, Taraxacum officinale Weber and so forth.

• Mycobiology
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• v.50 no.2
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• pp.121-131
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• 2022

The rare edible and medicinal fungus Antrodia cinnamomea has a substantial potential for development. In this study, Illumina HiSeq 2000 was used to sequence its transcriptome. The results were assembled de novo, and 66,589 unigenes with an N50 of 4413 bp were obtained. Compared with public databases, 6,061, 3,257, and 2,807 unigenes were annotated to the Non-Redundant, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases, respectively. The genes related to terpene biosynthesis in the mycelia of A. cinnamomea were analyzed, and acetyl CoA synthase (ACS2 and ACS4), hydroxymethylglutaryl CoA reductase (HMGR), farnesyl transferase (FTase), and squalene synthase (SQS) were found to be upregulated in XZJ (twig of C. camphora) and NZJ (twig of C. kanehirae). Moreover, ACS5 and 2,3-oxidized squalene cyclase (OCS) were highly expressed in NZJ, while heme IX farnesyl transferase (IX-FIT) and ACS3 were significantly expressed in XZJ. The differential expression of ACS1, ACS2, HMGR, IX-FIT, SQS, and OCS was confirmed by real-time quantitative reverse transcription PCR. This study provides a new concept for the additional exploration of the molecular regulatory mechanism of terpenoid biosynthesis and data for the biotechnology of terpenoid production.

• Korean Journal of Plant Taxonomy
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• v.48 no.1
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• pp.48-64
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• 2018

This study was carried out to investigate the flora of Oeumsan Mountain (Hongcheon-gun and Hoengseong-gun, Gangwon-do) from March of 2016 to September of 2017. The vascular plants were summarized into 509 taxa, including 98 families, 301 genera, 436 species, 4 subspecies, 57 varieties and 12 forms. Among the 509 investigated taxa, 9 Korean endemic, 1 endangered plant, 8 rare plants and 57 floristic regional indicator plants were also included. The naturalized plants encompassed 35 taxa. The percentage of naturalized plants species and the urbanization index were estimated to be 6.9% and 10.9%, respectively.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.1
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• pp.109-130
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• 2013

This study is to offer the raw data for conservation and restoration of national park by surveying and analysing the vascular plants in the Gayasan national park. The flora were summarized as 102 families, 328 genera, 469 species, 4 subspecies, 59 varieties and 9 forms. The endangered plants designated by Ministry of Environment were 2 taxa; Pedicularis hallaisanensis Hurus. and Gymnadenia conopsea for. albiflora Y.N.Lee. The rare plants designated by Korea Forest Service were 13 taxa; Crypsinus hastatus (Thunb.) Copel., Ligusticum tachiroei (Franch. & Sav.) M. Hiroe & Constance, Primula modesta var. fauriae (Franch.) Takeda, Lilium distichum Nakai ex Kamibay and so forth. The korean endemic plants were 15 taxa; Betula ermanii Cham., Carpinus laxiflora (Siebold & Zucc.) Blume, Stewartia pseudocamellia Maxim., Galium koreanum (Nakai) Nakai, Heloniopsis koreana Fuse, N.S. Lee & M.N. Tamura and so forth. The specific plants by floristic region were 66 taxa; Betula chinensis Maxim., Spiraea fritschiana Schneid., Vaccinium hirtum var. koreanum (Nakai) Kitam., Cimicifuga heracleifolia Kom., Adoxa moschatellina L. and so forth. The plants with approval for delivering oversea were 22 taxa; Salix hallaisanensis H.L$\acute{e}$v., Sanguisorba argutidens Nakai, Viola albida Palib., Weigela subsessilis (Nakai) L.H.Bailey and so forth. The naturalized plants were 30 taxa; Chenopodium album L., Lepidium apetalum Willd., Trifolium pratense L., Bidens frondosa L., Helianthus tuberosus L. and so forth.

• Korean Journal of Plant Resources
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• v.28 no.4
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• pp.487-510
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• 2015

This study was carried out to inventory the vascular plant flora in Deokjeokdo and its adjacent regions (Mungapdo, Soyado), Ongjin-gun, South Korea, from April to October 2014. Based on the voucher specimens, vascular plants in this area consisted of 108 families, 362 genera, 578 species, 5 subspecies, 66 varieties and 4 forms, totally 653 taxa. In the flora of this area, 5 taxa of Korean endemic plants were found distributed in the sites, including Hepatica insularis Nakai and Asarum glabrata (C.S.Yook & J.G.Kim) B.U.Oh. Korean rare and endangered plants found in this area were 1 taxa of Critical Endangered Species (CR) and 5 taxa of Vulnerable Species (VU). The floristic regional indicator plants found in this area were 67 taxa comprising 3 taxa of grade V, 2 taxa of grade IV, 12 taxa of grade III, 3 taxa of grade II and 47 taxa of grade I. Naturalized plants consisted of 67 taxa, such as Chenopodium album L. and Erigeron annuus (L.) Pers. were recognized widely distributed in the area.